Typhonium giganteum extract inhibits human gastric carcinoma cell proliferation and metastasis

Abstract:

Objective：To examine the effects of Typhonium giganteum extract（TGE） on the proliferation and metastasis of human gastric carcinoma cell line GC9811 and to elucidate its possible mechanism of action. Methods：TGE was isolated and purified by phyto－chemistry，and the inhibitory effect of different concentrations of TGE on GC9811 cell proliferation at various time points was mea－sured by MTT colorimetric assay. The effect of TGE on cell migration was determine by cell scratch assay and Transwell migration assay. The mRNA and protein expression of matrix metalloproteinase-9（MMP-9），matrix metalloproteinase-2（MMP-2），and E-cad－herin was measured by real-time quantitative PCR and Western blot，respectively. Results：The MTT assay showed that TGE significantly inhibited the growth of GC9811 cells in a dose-dependent manner. The cell scratch and Transwell migration assays demonstrated that the migratory ability of GC9811 cells decreased as TGE concentration increased. Furthermore，MMP-2 and MMP-9 expression was significantly downregulated and E-cadherin expression was significantly upregulated in the 30 g/L TGE group than in the 15 g/L TGE group. Conclusion：TGE can significantly inhibit the proliferation and metastasis of GC9811 cells，and its mechanism of action may be related to the upregulation of E-cadherin expression and downregulation of MMP-9 and MMP-2 expression. TGE is a highly potent and minimally toxic antitumor lead compound worthy of further investigation.