Value of preoperative expression levels of serum miR-148a-3p and mR-139-5p on prediction of recurrence and metastasis in prostate cancer patients

Abstract:

Objective：To investigate the value of expression levels of serum miR-148a-3p and miR-139-5p on postoperative recur－rence and metastasis in prostate cancer（PCa） patients. Methods：A total of 142 patients with PCa admitted to our hospital from January 1st，2015 to December 31st，2017 were selected and divided into the non-recurrence and metastasis group（n=84） and the recurrence and metastasis group（n=58） according to their postoperative condition. The expression level of preoperative miR-148a-3p，miR-139-5p and prostate specific antigen（PSA） in two groups were detected，and their relationship with clinicopathological characteristics was analyzed. ROC curve was used to analyze the value of miR-148a-3p，miR-139-5p and PSA in predicting postoperative recurrence and metastasis of PCa. Pearson correlation analysis was used to analyze the correlation among miR-148a-3p，miR-139-5p and PSA. Results：Levels of serum miR-148a-3p，miR-139-5p and PSA in the recurrence and metastasis group were （4.80±0.75） ng/mL，（7.64±2.38） ng/mL，（63.75±14.92） ng/mL，respectively，which were significantly higher than those in the non-recurrence and metas－tasis group of （2.15±0.36） ng/mL，（3.50±0.62） ng/mL，（18.72±8.60） ng/mL，with statistically significant differences（t=10.648，14.715，and 9.804，P=0.000）. Expressions levels of serum miR-148a-3p and miR-139-5p in patients with recurrence and metastasis were correlated with tumor stage（t=5.117，P=0.024；t=5.304，P=0.018），Gleason score（t=5.512，P=0.006；t=6.218，P=0.000） and PSA level（t=10.208，P=0.000；t=13.620，P=0.000）. ROC curve analysis showed that the best cut-off values of serum miR-148a-3p，miR-139-5p and PSA for predicting postoperative recurrence and metastasis of PCa were 3.40，5.38 and 36.80 ng/mL，respectively. The area under curve（AUC）（0.914，95%CI=0.858 to 0.972） of postoperative recurrence and metastasis predicted by the three combinations was the highest，with the sensitivity of 92.0% and the specificity of 83.6%. Correlation analysis showed that expres－sion levels of serum miR-148a-3p and miR-139-5p were positively correlated with PSA in patients with recurrence and metastasis（r=0.774，P<0.001；r=0.806，P<0.001）. Conclusion：The up-regulated expression of serum miR-148a-3p and miR-139-5p before surgery is related to the recurrence and metastasis after PCa surgery，and the test combined with PSA has a high value in predicting the recurrence and metastasis after PCa surgery.