Abstract:#$NLObjective：To detect the expression of microRNA-496(miR-496) in ovarian serous carcinoma, analyze its clinical significance, analyze its correlation with homologous heteroprotein SIX1(SIX1). Methods：75 cases of ovarian serous carcinoma were selected as the observation group, 75 cases of ovarian serous cystic adenoma were selected as the control group. Expression of miR-496 was detected by real-time fluorescence quantitative PCR in two groups. Expression of PCNA and BAX was detected by immunohistochemical method in the observation group. Expression of SIX1 was detected by Western Blot method in the observation group. Results：Expression of miR-496 was lower in the observation group than that in the control group(P<0.05). The expression of miR-496 was statistically significant in different tumor maximum diameter, histological grade, vascular recidivism, bilateral occurrence, lymph node metastasis and different TNM stages in the observation group(P<0.05). Expression of miR-496 is related to survival time(P<0.05). Negative correlation were found between miR-496 and PCNA(r=-0.54,P=0.018), miR-496 and SIX1(r=-0.58, P=0.013), positive correlation were found between miR-496 and BAX(r=0.52,P=0.011). Conclusion：Expression of miR-496 of ovarian serous carcinoma is decreased, which has a certain regulatory effect on the formation and development of the tumor. Expression of miR-496 has a certain significance for judging the prognosis. MiR-496 not only regulates cell proliferation and apoptosis, but also may play a role in the regulation of SIX1.
