Expression and significance of CX3CL1 in brain tissue in mutant P301L transgenic mice

Abstract:

Objective：To investigate the expression and function of CX3CL1，an inflammatory chemokine，in the brain tissue of mutant P301L transgenic mice. Methods：A total of 10 mutant P301L transgenic mice aged 9 months and 10 wild-type mice aged 9 months were selected. Western blot was used to measure the expression of CX3CL1 in brain tissue. Brain tissue samples were collected to pre－pare frozen sections，and immunofluorescence assay was performed with anti-p-tau and anti-CX3CL1 antibodies. Results：Compared with the wild-type mice，the transgenic mice had a significant increase in the expression of CX3CL1 in brain tissue. The immunofluo－rescence assay showed the co-expression of CX3CL1 and p-tau. Conclusion：CX3CL1 is significantly up-regulated in the brain tissue of mutant P301L transgenic mice and may play a role in the inflammatory mechanism of nerve injury induced by tau.