A novel strategy based on proximity binding-induced entropy-driven amplification circuits for high-sensitivity detection of Helicobacter pylori DNA

Abstract:

Objective：To establish a novel strategy based on proximity binding-induced entropy-driven amplification circuits for high-sensitivity detection of DNA. Methods：Target DNA was used to trigger proximity binding to form a sandwich complex with two affinity probes and then a proximity trigger，which lit up the entropy-driven amplification circuits by mediating branch migration for DNA detection. Results：Under the optimal experimental conditions，the DNA sensing strategy established in this study showed high sensi－tivity and selectivity，with a wide dynamic range of 0.01-100 nmol/L and a limit of detection as low as 6.7 pmol/L. Conclusion：This rapid，cost-effective，and highly efficient signal amplification strategy provides a simple and sensitive platform for DNA detection.