Association of KDM5C with SAC on mitosis of osteosarcoma

Abstract:

Objective：To investigate the alteration of Lysine-specific demethylase 5C（KDM5C） protein level and the regulatory effect of KDM5C on spindle assembly checkpoint（SAC），related mitotic progression of osteosarcoma cell lines. Methods：The various osteosar－coma cells in prometaphase were collected by synchronization. The alteration of KDM5C protein level and its potential posttransla－tional modification in mitosis were determined by Western blot. The effect of inhibitor CPI-455 on mitosis process was detected using Western blot. Results：In mitosis，the alterations of SAC related markers，cell division cycle protein 27（cdc27） and Cyclin B1，were similar to previous literatures，but KDM5C protein level had no obvious change. The shift and ubiquitination of KDM5C truncations were not observed by Western bolt. When demethylase activity of KDM5C was inhibited by CPI-455，SAC related markers，cdc27 and Cyclin B1，had no obvious change. Conclusion：Mitotic process of osteosarcoma cell and the SAC is unaffected by KDM5C protein level and its demethylase activity.