Effect of phospholipase PLCD1 on epithelial-mesenchymal transition of pancreatic cancer cells CAPAN-1 and BXPC-3

Abstract:

Objective：To investigate the effects of the phospholipase Cδ1（PLCD1），a potential tumour gene，in pancreatic cancer cells，on the proliferation，migration and invasion of pancreatic cancer cells. Methods：Liposome method was used to transfer the pcDNA3.1-PLCD1 to pancreatic cancer cells and the Vector group with empty vector was set. The proliferation,migratory and invasive abilities of pancreatic cancer cells were detected with CCK-8 assay，clone formation assay，Transwell assay and wound healing assay. In additional，Western blot was used to demonstrate the potential mechanism. Results：PLCD1 was significant up-regulated in CAPAN-1 and BX－PC-3 cells after being transiently transfected with the PLCD1 over-expressing plasmid（P＜0.05）. The ability of proliferation showed significant inhibition with CCK-8 and clone formation assay（P＜0.05）. The motility，invasion and migration were remarkable restrained with Transwell assay and wound healing assay（P＜0.05）. Additionally，Western blot showed PLCD1 induced MAPK/ERK pathway molecule p-ERK1/2（P＜0.05），as well as the mesenchymal markers N-cadherin and Vimentin were decreased（P＜0.05）. And the ex－pression of the epithelial marker E-cadherin was increased（P＜0.05）. Conclusion：PLCD1 may inhibit the proliferation，migration and invasion of pancreatic cancer cells through inhibiting the MAPK/EKR pathway and suppressing the EMT，which lay the foundation for the further study of PLCD1 in pancreatic cancer.