GLP-1improvestheapoptosisofisletβcellsintype2diabetesviathePar-4/NF-κBpathway

Abstract:

Objective：Toobservetheeffectofglucagon-likepeptide-1（GLP-1）ontheexpressionofPar-4/NF-κBandtheapoptosisofisletβcellsindiabeticmice.Methods：Experimentalmicewererandomlydividedintonormalcontrolgroup（Cgroup），Par-4knockoutgroup（CPgroup），diabetesgroup（Dgroup），diabetesandPar-4knockoutgroup（DPgroup），GLP-1+diabetesgroup（GDgroup），andGLP-1+diabetesandPar-4knockoutgroup（GDPgroup），with8miceineachgroup.Foreachgroup，theapoptosisrateofisletβcellswasdeterminedbyTUNELassay，theproteinexpressionlevelsofPar-4andNF-κBweredeterminedbyWesternblot，andinsulinsecretionwasmeasuredbyELISA.Results：TherewerenosignificantdifferencesbetweentheCPgroupandtheCgroupintheapoptosisrateofisletβcells（P=0.965），NF-κBexpression（P=0.754），andinsulinsecretion（P=0.797）.ComparedwiththeCgroupandCPgroup，theDgrouphadsignificantlyincreasedapoptosisrate（P=0.000）andexpressionofPar-4（P=0.000）andNF-κB（P=0.000），butsignificantlyreducedinsulinsecretion（P=0.000）.ComparedwiththeDgroup，theDPgroupandGDgrouphadsignifi－cantlydecreasedapoptosisrate（P=0.000，P=0.000）andexpressionofPar-4（P=0.000，P=0.000）andNF-?资B（P=0.000，P=0.002），butsignificantlyimprovedinsulinsecretion（P=0.000，P=0.026）.ComparedwiththeGDgroup，theGDPgrouphadsignificantlydecreasedapoptosisrate（P=0.000）andexpressionofPar-4（P=0.000）andNF-κB（P=0.015），butsignificantlyimprovedinsulinsecretion（P=0.026）.TherewerenosignificantdifferencesbetweentheDPgroupandtheGDPgroupintheapoptosisrateofisletβcells（P=0.930），theexpressionofPar-4（P=0.965）andNF-κB（P=0.875），andinsulinsecretion（P=0.797）.Conclusion：GLP-1improvestheapoptosisofisletβcellsandinsulinsecretionintype2diabetesbyinhibitingtheexpressionofPar-4/NF-κB.