Relationshipbetweenthechangesinirisinlevelandatherosclerosisindicesinpatientswithtype2diabetesmellitustreatedwithmetformin

Abstract:

Objective：Toexploretheeffectofmetformininterventiononserumirisinlevelsinpatientswithnewlydiagnosedtype2diabetesmellitus（T2DM）anditsroleindelayingatherosclerosis（AS）.Methods：Onehundredandtwentypatientswithnewlydiag－nosedT2DMwererandomlydividedintotwogroups，namelylow-dosegroupandhigh-dosegroup，with60casesineachgroup.Boththelow-dosegroup（250mg，tid）andthehigh-dosegroup（500mg，tid）weretreatedwithmetforminfor6months.Ｃarotidin－tima-mediathickness（CIMT）wasmeasuredbyultrasound.Thelevelsofmatrixmetalloprotein9（MMP9）andotherbiochemi－calindexessuchasfastfoodglucose（FBG），triacylglycerol（TAG）andbodymassindex（BMI）werecomparedbeforeandaftertreatment.Results：Beforetreatment，therewasnosignifi－cantdifferenceinbaselinedatabetweenthetwogroups（P>0.05）.Aftertreatment，thelow-dosegrouphadasignificantlyincreasedlevelofserumirisin（215.17±89.96vs.356.66±102.32，P<0.05），whilethehigh-dosegroupshowedasignificantimprovementinCIMT（1.43±0.89vs.1.10±1.21，P<0.05）inadditiontoasignificantincreaseinserumIrisinlevel（238.17±67.96vs.389.43±93.43，P<0.05）.Meanwhile，thereweresignificantreductionsinthelevelsofMMP-9andoxidativestressinbothgroups（P<0.05）.Comparedwiththelow-dosegroup，thehigh-dosegrouphadsignificantlygreaterreductionsinMMP9andmalondialdehydeandasignificantlygreaterincreaseinserumIrisin（P<0.05）.Correlationanalysisshowedthatafter6months，ΔCIMTinthehighdosegroupwasnegativelycorrelatedwithserumΔirisin，andpositivelycorrelatedwithΔBMI，ΔFBG，ΔTGandchangeofoxidativestresslevel.ΔMMP9wasnegativelycorrelatedwithserumΔirisinandpositivelycorrelat－edwithΔFPGandchangeinoxidativestresslevel.ΔIrisinwasnegativelycorrelatedwiththechangeinoxidativestresslevel.Fur－thermore，multivariatelinearstepwiseregressionanalysiswasperformedwithΔCIMTandΔMMP9inthehigh-dosegroupasdepen－dentvariables，andotherrelatedfactorsasindependentvariables.TheresultsshowedthatΔirisinandchangeinoxidativestresslevelwereindependentinfluencingfactorsforΔCIMTandΔMMP9.Conclusion：Metformincandelaytheprogressionofcarotidatheroscle－rosisinpatientsnewlydiagnosedwithT2DM，partlyduetotheelevatedirisinlevelandimprovedoxidativestress.