RelationshipbetweenILC2sandmetabolicsyndromeinhemodialysispatientswithdiabeticnephropathy

Abstract:

Objective：Toinvestigatetherelationshipbetweentype2innatelymphoidcells（ILC2s）andmetabolicsyndrome（MS）inhemodialysispatientswithdiabeticnephropathy（DN）.Methods：Atotalof105hemodialysispatientsinourhospitalwereenrolledinthisstudy，including40DNpatients（hemodialysis+DNgroup）and65patientswithnon-diabeticnephropathy（hemodialysis+NDNgroup）.Theclinicaldatawerecollected.Anthropometricparameters，bloodglucose，andbloodlipidsweremeasured.And35patientswithtype2diabetesalone（diabetesgroup）and30outpatientsreceivingphysicalexamination（healthycontrolgroup）wereselectedascontrol.ThemRNAexpressionlevelsofILC2srelatedfactorsinperipheralbloodmononuclearcellsweredeterminedbyquantitativereal-timePCR.ILC2s%wasmeasuredbyflowcytometry.TheproteinexpressionlevelsofILC2srelatedcytokines（interleukin[IL]-5andIL-13）inplasmaweremeasuredbyenzyme-linkedimmunosorbentassay.Results：Thehemodialysis+DNgrouphadasignificant－lyhigherincidencerateofMScomparedwiththehealthycon－trolgroup，thediabetesgroup，andthehemodialysis+NDNgroup（87.5%vs.0%，57.10%，and50.80%，?字2=53.084，P=0.000）.Com－paredwiththehealthycontrolgroup，thehemodialysis+DNgrouphadsignificantlyhighermRNAexpressionlevelsofILC2schar－acteristictranscriptionfactorROR-alpha，membranereceptorT1/ST2，IL-17B，IL-5，andIL-13（P=0.000，P=0.000，P=0.000，P=0.000，P=0.004）andhadsignificantlyhigherproteinexpressionlev－elsofIL-5andIL-13（P=0.012，P=0.076）.Theflowcytometryresultsshowedthatthehemodialysis+DNgrouphadasignificantlyhigherILC2s%thantheotherthreegroups（F=185.287，P=0.000）.LogisticregressionanalysisshowedthatwiththeincreasingILC2sactivity，thehemodialysis+DNgrouphadahigherprobabilityofMSthantheotherthreegroups.CorrelationanalysisindicatedthatILC2swaspositivelycorrelatedwithwaistcircumferenceandtriglyceridelevelinhemodialysispatientswithDN（r=0.356，P=0.024；r=0.462，P=0.003）.Conclusion：HemodialysispatientswithDNhaveahigherprevalenceofMS.ILC2sappearstobethedominantactivationstateinhemodialysispatientswithDN.ILC2srelatedfactorsmaybeinvolvedintheformationofMSinhemodialysispatientswithDNthroughtheeffectsonabdominalobesityandlipidmetabolism.