Role of serum interleukin-27 concentration in early diagnosis of type 2 diabetic nephropathy

Abstract:

Objective：To assess the level and significance of interleukin-27（IL-27） in patients with type 2 diabetes mellitus （T2D） and diabetic nephropathy（DN）. Methods：A total of 116 patients with T2D were divided into diabetic normal urinary albumin group （DN0 group），early diabetic nephropathy group（DN1 group） and clinical diabetic nephropathy group（DN2 group） in accordance with urinary protein；healthy people were selected as the control group（H group）. Level of IL-27 was detected by enzyme-linked im－munosorbent assay（ELISA）. Hypersensitive C-reactive protein（hs-CRP），urinary albumin/creatinine，serum creatinine，triglyceride and total cholesterol were detected by automatic biochemical analyzer；glycosylated hemoglobin level was detected by high perfor－mance liquid chromatography；C-peptide level was detected by chemiluminescence；correlation between serum IL-27 concentration and those above indicators was analyzed. Results：IL-27 serum concentration of patients in the DN0 group[788.200（594.500，1010.950） pg/mL] was significantly higher than that in the H group[546.200（411.600，639.250） pg/mL]，with statistically significant difference（P=0.003）. IL-27 concentration in the DN1 group[990.780（811.480，1237.830） pg/mL] was significantly higher than that in the DN0 group，with statistically significant difference（P=0.028）. IL-27concentration in DN2 group[1213.300（996.450，1 546.200） pg/mL] was significantly higher than that in the DN1 group，with statistically significant difference（P=0.037）. IL-27 serum concentration had a positive relationship with urine protein（r=0.580，P=0.000），serum creatinine（r=0.257，P=0.002），glycosy－lated hemo-globin（r=0.343，P=0.000），serum hs-CRP（r=0.431，P=0.000） and fasting blood glucose concentration（r=0.334，P=0.000）；had no significant correlation with triglyceride，total cholesterol and serum C-peptide（P>0.05）. IL-27，as a serum marker of DN patients，had a higher potency than hs-CRP；combination of two markers was able to increase the diagnostic efficiency. Conclusion：Serum concentration of IL-27 is associated with the development of DN in patients with T2D. IL-27 may provide references to early diagnosis of DN patients.