Expression of miR-30-5p in esophageal cancer and its effect on proliferation and migration of esophageal cancer cells

Abstract:

Objective：To investigate the effect of miR-30-5p on the proliferation and migration of esophageal cancer cells and its molecular mechanism. Methods：A total of 120 samples of esophageal cancer and 120 samples of adjacent tissues were collected in department of cardiothoracic vascular surgery of Kaifeng Central Hospital and thoracic surgery of Henan People’s Hospital from June 2014 to June 2019，and fluorescence quantitative PCR was used to detect the expression of miR-30-5p. Lentivirus was used to estab－lish miR-30-5p overexpression cell lines（the miR-30-5p group） and control cell lines（the miR-control group） in esophageal cancer cell line EC109. The EdU staining was used to analyze the proliferation in two groups. Transwell was used to analyze migration in two groups. Bioinformatics and double luciferase reporter gene were used to analyze target genes of miR-30-5p. Influence of overexpres－sion gene on cell proliferation and migration in the miR-30-5p group and the miR-control group was analyzed. Results：Compared with adjacent tissues，the expression of miR-30-5p of esophageal cancer tissues was significantly increased，with statistically signifi－cant difference（P<0.05）. Compared with the miR-control group，the proliferation and migration ability in the miR-30-5p group signifi－cantly decreased，with statistically significant difference（P<0.05）. Bioinformatics and double luciferase reporter gene showed that BCL-9 was the target gene of miR-30-5p. Overexpression of BCL-9 in the miR-control group was able to significantly increase cell proliferation and migration，with statistically sig－nificant difference（P<0.05）. Over-expression of BCL-9 in the miR-30-5p group had no statistical significance on cell proliferation and migration（P<0.05）. Conclusion：The expression of miR-30-5p is low in esophageal cancer，which can up-regulate the expression of its target gene BCL-9 and promote proliferation and mi－gration of esophageal cancer cells.