The miR-206 inhibits proliferation，migration and invasion of prostate cancer cells by targeting PDCD10

Abstract:

Objective：To investigate the effect of miR-206 on proliferation，invasion and migration of prostate cells by regulating pro－grammed cell death 10（PDCD10）. Methods：Prostate cancer specimens of 61 cases in Sinopharm Hanjiang Hospital were selected and 40 benign prostatic hyperplasia tissues were selected as controls. Expression levels of miR-206 and PDCD10 were detected by in situ hybridization，and the relationship between expressions of miR-206 or PDCD10 and clinicopathological parameters of patients with prostate cancer was analyzed. The interaction between miR-206 a and PDCD10 were verified by dual luciferase reporter gene assay. Prostate cancer PC3 cells were transfected into miR-206 mimics+PDCD10 pcDNA overexpression vector，miR-206 mimics，PDCD10 pcDNA overexpression vector，the mimics NC group and the mimics NC+PDCD10 pcDNA group，respectively. The expres－sion of PDCD10 mRNA was detected by RT-qPCR and the expression of PDCD10 protein was detected by Western blot. The proliferation，migration and invasion of prostate cancer cells were detected by MTT and Transwell. Results：In situ hy－bridization showed that the positive expression rate of miR-206 in prostate cancer tissues（24.59%，15/61） was significantly lower than that in benign prostatic hyperplasia tissues（87.5%，35/40）（χ2=38.248，P=0.000）. The positive expression rate of PDCD10 in prostate cancer tissues（73.77%，45/61） was significantly higher than that in benign prostatic hyperplasia tissues（27.5%，11/40）（χ2=20.397，P=0.000）. Both miR-206 and PDCD10 were associated with TNM clinical stage and Gleason score（P<0.05），and were not associated with age and preoperative PSA levels（P>0.05）. Linear regression and correlation analysis showed that miR-206 expression had a significant negative correlation with PDCD10 expression（r=-0.959，P<0.001）. After co-transfection of miR-206 mimic with the PDCD10 wild-type reporter vector，the luciferase activity in cells was decreased（P<0.01）. The expression level of PDCD10 mRNA and protein was the lowest in the miR-206 mimics group. Overexpression of miR-206 significantly inhibited the proliferation，migration and invasion of PC3 cells. Overexpression of PDCD10 was able to significantly increase the proliferation，migration and invasion of PC3 cells，and overexpression of PDCD10 was able to reverse the inhibitory effect of miR-206 on the cell proliferation，migration and invasion abilities of PC3 cells. Conclusion：The miR-206 can inhibit the proliferation，migration and invasion of prostate cancer cells，whose mechanism is related with the targeted negative regulation of PDCD10 expression.