Therapeutic effect of low intensity ultrasound-mediated nanobubbles loaded with double suicide gene on bladder cancer in mice

Abstract:

Objective：To prepare nanobubbles loaded with colicytocinedeaminase-thymidinekinase（CD-TK） for treating bladder cancer in mice. Methods：Nanobubbles loaded with CD-TK double suicide gene were prepared by thin layer method and cationic nanobub－bles loading CD-TK double suicide gene were synthesized. Nanobubbles’ shape was observed through light microscope and the par－ticle diameter was measured via Malvin laser particle size analyzer. The mice model with bladder cancer was established，ultrasound was used to irradiate the tumor site and the double suicide gene was transfected. Intraperitoneal injection of atoxic prodrug was con－tinuously given to each group for one week. The longest and shortest diameters of each nude mouse’s tumor were recorded，and the tumor’s volume changes were calculated and recorded. Tumor specimens were taken at the fifth week. Apoptosis and proliferation of tumor cells were evaluated by immunofluorescence. Results：The nanobubbles loading CD-TK double suicide gene were successfully prepared in this study. Ultrasound-mediated nanobubbles loaded with CD-TK can successfully transfect suicide gene，inhibiting the proliferation and apoptosis of tumors. Conclusion：Nanobubbles loaded with CD-TK may be a new therapy for bladder cancer.