Ovarian functional reconstruction in rats with premature ovarian failure through hyperbaric oxygen combined with bone marrow mesenchymal stem cell transplantation

Abstract:

Objective：To study the ovarian functional reconstruction in rats with premature ovarian failure（POF） through hyperbaric oxygen（HBO） combined with bone marrow mesenchymal stem cells（BMSCs） transplantation. Methods：Female sprague dawley（SD） rats were subjected to intraperitoneal injection of cyclopho sphamide（CTX） to induce POF. Successful modeling（36 rats） were ran－domly divided into the model group，the BMSCs transplantation group，and HBO+ BMSCs transplantation group，12 in each group. Another 12 rats were taken from the blank group. After the BMSCs transplantation group and the HBO+BMSCs transplantation group were given individual intervention，rats estrus cycle，serum E2，FSH hormone level，the amount of follicles at all levels were recorded respectively，and ovarian microstructure，distribution of transplanted cells in ovarian tissue and reproductive ability were observed so as to evaluate the ovarian function. Results：The recovery of estrus cycle in the HBO+BMSCs transplantation group was better than that in the BMSCs transplantation group. E2 in the HBO+BMSCs transplantation group（82.463±4.108） was higher than that in the BMSCs transplantation group（77.233±3.714）（P=0.047） and FSH in the HBO+BMSCs transplantation group（21.292±2.458） was lower than that in the BMSCs transplantation group（24.574±2.389）（P=0.043）. Compared with the BMSCs transplantation group （13.50±1.43），（5.40±0.70），the number of GF（11.50±1.27） MF（4.50±0.53） in the HBO+BMSCs transplantation group increased at varying degrees（P=0.024，0.028）. Compared with the BMSCs transplantation group（41.30±3.20，13.30±1.89），the number of PF（46.00±3.74），AF（16.10±2.18） in the HBO+BMSCs transplantation group decreased at varying degrees（P=0.044，0.040）. The ex－pression of green fluorescence in the HBO+BMSCs transplantation group was more than that in transplantation group after 30 days of transplantation. SD rats in the HBO+BMSCs transplantation group had more offspring than that in the BMSCs transplantation group. Conclusion：The HBO+BMSCs transplantation has a synergistic effect on functional reconstruction of POF，and it may play a safe and effective role in the treatment of POF．