Long-chain non-coding PANDAR regulates proliferation and metastasis of thyroid carcinoma by targeting miR-637

Abstract:

Objective：To explore the molecular mechanism of long-chain non-coding RNA PANDAR（LncRNA PANDAR） to promote the growth and metastasis of thyroid cancer. Methods：Firstly，real-time quantitative PCR was used to detect the expression of PANDAR in serum and cancer tissues of patients with thyroid cancer；measures like MTT，flow cytometry and Transwell were used to detect the effect of PANDAR on growth，apoptosis and migration of thyroid cancer cells. The dual luciferase reporter gene assay was used to determine that miR-637 was the target of PANDAR and to determine the role of miR-637 in PANDAR on growth，apoptosis and mi－gration of thyroid cancer cells. Results：Expression of PANDAR was significantly increased in serum and cancer tissues of patients with thyroid cancer，and PANDAR was able to significantly promote cell growth and migration，and inhibit apoptosis. The target of PANDAR was miR-637 and miR-637 was able to reverse PANDAR-induced cell proliferation，metastasis and apoptosis. Conclusion：PANDAR regulates proliferation and metastasis of thyroid cancer by targeting miR-637.