Correlation analysis between lymph node metastasis and microRNA expression in gastric cancer

Abstract:

Objective：To investigate the correlation between lymph node metastasis and microRNA expression in gastric cancer，so as to find the potential target of gastric cancer treatment. Methods：The clinical pathological information and miRNA expression of gastric cancer patients from the public database TCGA were downloaded. Patients were divided into the lymph node metastasis positive group and the lymph node metastasis negative group in accordance with the lymph node metastasis. Independent sample t-test was used to evaluate the continuous variable data of the two groups，chi square test was used to evaluate the difference between the two groups，and factors with single factor analysis P≤0.01 were included into the logistic multiple factor regression analysis. Results：Single factor analysis indicated that the expression level of 71 miRNAs had statistically significant difference between the two groups，and tumor T stage and tumor differentiation had significant difference between the two groups. Logistic regression analysis showed that：hsa-mir-3940（OR=0.713，95%CI=0.524-0.970），hsa-mir-495（OR=1.479，95%CI=1.115-1.963），hsa-mir-580（OR=0.646，95%CI=0.441-0.946），hsa-mir-6720（OR=1.366，95%CI=1.018-1.834），hsa-mir-935（OR=0.862，95%CI=0.747-0.994），T stage（OR=2.831，95%CI=1.728-4.639）. Conclusion：The expression of hsa-mir-3940，hsa-mir-495，hsa-mir-580，hsa-mir-6720，hsa-mir-935 and T stage in the gastric cancer tissue are independent influencing factors of lymph node metastasis.