Expression of eukaryotic translation initiation factor 2c and its relationship with apoptosis in colorectal adenocarcinoma

Abstract:

Objective：To detect the expression of eukaryotic translation initiation factor 2c（eIF2c） in colorectal adenocarcinoma and to analyze its relationship with apoptosis. Methods：A total of 69 patients with colorectal adenocarcinoma were taken as the study ob－ject. Tumor tissue was taken as the observation group and normal colonic mucosa tissue was taken as the control group. The expres－sions of eIF2c and Caspase-3 were detected by immunohistochemistry method. Expression of Bcl-2 associated X protein（BAX） was detected by Western blot. Colon cancer SW480 cell line of overexpression eIF2c was constructed and expression of BAX was detected by Western blot. Results：Differences of positive rate in two groups was statistically significant（56.5% vs. 13.0%， χ2=28.750，P=0.000）. Expression of eIF2c was significantly different in maximum diameter（68.1% vs. 31.8%， χ2=8.021，P=0.005），invasion depth（81.2% vs. 35.1%， χ2=14.849，P=0.001），necrosis（48.0% vs. 78.9%， χ2=5.366，P=0.021），lymph node metastasis（83.3% vs. 42.2%， χ2=10.765，P=0.001） and tumor thrombus（87.5% vs. 47.2%， χ2=8.134，P=0.004）. Negative correlation was found between eIF2c and Caspase-3（r=-0.69，P=0.000），and eIF2c and Bax（r=-0.66，P=0.000）. According to survival analysis，eIF2c was related with survival time（ χ2=14.00，P=0.000）. Expression of BAX was decreased in colon cancer cell line of overexpressed eIF2c. Conclusion：Higher expression of eIF2c is related with clinicopathological features and prognosis in colorectal adenocarcinoma. Abnormal expression of eIF2c is re－lated with apoptosis factors.