Correlation between microRNA expression and early recurrence of hepatocellular carcinoma

Abstract:

Objective：To investigate the correlation between expression of microRNA（mRNA） and early recurrence of hepatocellular carcinoma（HCC）. Methods：The clinical information，follow-up results and the expression of miRNAs in TCGA were downloaded from the public database. According to the recurrence time，patients were divided into the early-stage recurrence group（≤2 years recur－rence） and the late-stage recurrence group（>2 years recurrence）. Independent sample t test was used to assess the difference of con－tinuous variable data in two groups，and chi-square test was used to evaluate the difference of categorical variable data in two groups. Factors（P<0.01） was included in logistic multivariate regression analysis. Results：T-test analysis showed that the miRNA（P≤0.01） was hsa-mir-550a-1，hsa-mir-550a-2，hsa-mir-550a-3，hsa-mir-331，hsa-mir-514a-1，hsa-mir-210，hsa-mir-155，hsa-mir-mir-3170，hsa-mir-4677，hsa-mir-4677，hsa-mir-508，hsa-mir-514a-3，hsa-mir-514a-2 and hsa-mir-624. The chi-square test showed that the indicator（P≤0.01） was tumor T stage. Logistic multivariate regression analysis showed that hsa-mir-155（OR=0.660，95%CI=0.519-0.841），hsa-mir-331（OR=1.475，95%CI=1.004-2.168），hsa-mir-514a-1（OR=0.603，95%CI=0.413-0.879），hsa-mir-550a-1（OR=1.680，95%CI=1.034-2.703） and tumor T stage（OR=1.640，95%CI=1.126-2.389） were independent influence factors of early recurrence，with statistically significant differences（P<0.05）. Conclusion：Expression levels of hsa-mir-155，hsa-mir-331，hsa-mir-514a-1 and hsa-mir-550a-1 in HCC tissues and tumor T stage are independent factors influencing early recurrence of postop－erative HCC.