Effects of circular non-coding RNA hsa_circ_001842 on proliferation，apoptosis，invasion and tumorigenicity of renal cancer cells

Abstract:

Objective：To investigate the expression of circular non-coding RNA hsa_circ_001842 as an oncogene in renal cancer tissues and renal cells of patients with renal cell carcinoma（RCC），and to investigate its effects on the proliferation，apoptosis，invasion and tumorigenicity. Methods：A total of 64 cases of renal cancer specimens and adjacent tissue specimens resected from September 2018 to October 2019 in Mianyang Central Hospital were collected. The qRT-PCR was used to detect the expression of all RCC tissues and adjacent normal tissues. Caki-1 kidney cancer cells were divided into ox-NC group（transfected with over-express－ing empty plasmid），ox-circ_001842 group（transfected with circ_001842 over-expression plasmid），sh-NC group（transfected with silent empty plasmid），and sh-circ_001842 group（trans－fected with circ_001842 silencing plasmid）. Cell clone formation experiment，flow cytometry，cell scratch test and cell invasion experiment were used to detect the effect of hsa_circ_001842 on the proliferation，apoptosis，migration and invasion of RCCs. Western blot was used to detect the expression of epithelial mesenchymal transition（EMT）-related protein and MMP-9 protein in RCCs. Caki-1 cells transfected with sh-NC and sh-circ_001842 were inoculated subcutaneously in nude mice，and tumor volume and weight were measured every 7 days. Mice in each group were executed after 4 weeks and immunohistochemical techniques and qRT-PCR were used to detect the expression of EMT-related proteins in tumor tissues. Results：The expression of hsa_circ_001842 in renal cancer tissues of renal cell carcinoma patients was significantly higher than that in normal adjacent tissues（P<0.05）. Compared with the ox-NC group and the sh-circ_001842 group，the Caki-1 renal cell’s colony formation rate，wound healing ratio，cell invasion ability and E-cadherin protein in the ox-circ_001842 group and the sh-NC group were significantly increased（P<0.05），while the apoptosis rate，N-cadherin and MMP-9 protein expression were significantly reduced（P<0.05）. Nude tumor formation experiments showed that the tumor volume and weight in the sh-circ_001842 group were smaller than those in the sh-NC group（P<0.05），and the expression level of E-cadherin protein increased significantly，while expression level of N-cadherin protein was decreased significantly（P<0.05）. Conclusion：Expression of hsa_circ_001842 in renal cancer tissues and cells is up-regulated，inhibiting the proliferation，migration，invasion and tumorigenicity by inhibiting the expression of hsa_circ_001842.