Alleviation of hypoxia/reoxygenation injury of cardiomyocytes cultured in vitro with paeonol by inhibiting miR-155-5p

Abstract:

Objective：To investigate the effect of paeonol on H9C2 cells damaged by hypoxia/reoxygenation and its underlying mech－anism. Methods：H9C2 cells were pre-treated with paeonol or not，and the hypoxia/reoxygenation injury model of H9C2 cells in vitro was established. Expression level of miR-155-5p was measured by qRT-PCR. Proliferation activity and apoptosis level of H9C2 cells were validated using MTT assay and flow cytometry. ELISA kits were used to detect myocardial injury markers creatine kinase（CK）and lactate dehydrogenase（LDH）. Results：Paeonol significantly enhanced the activity of H9C2 cells and decreased the level of apop－tosis and the release of CK and LDH after hypoxia/reoxygenation treatment. Paeonol notably inhibited the expression of miR-155-5p in H9C2 cells. It was observed that inhibition of miR-155-5p alone had similar effects on H9C2 cells with paeonol. After paeonol pre-treatment and miR-155-5p mimics transfection at the same time，effects of paeonol on H9C2 cells were reversed by over-ex－pressed miR-155-5p. Conclusion：Paeonol can alleviate injuries in H9C2 cells induced by hypoxia/reoxygenation by down-regulating the level of miR-155-5p.