Inhibitory effect of resveratrol on proliferation of ovarian cancer cells by down-regulating ILK/β-catenin signaling pathway

Abstract:

Objective：To investigate the effect of resveratrol on the proliferation of ovarian cancer A2780 cells，and to explore its possible mechanism. Methods：Human ovarian adenocarcinoma A2780 cells were cultured in vitro and treated with different concen－trations of resveratrol（50，100，200，400 μmol/L） for 24，48 and 72 h. MTT assay was used to detect cell proliferation and cell cycle changes was determined by flow cytometry. Western blot was used to detect the expression of integrin-linked kinase（ILK），β-catenin and Cyclin D1 in cells. Results：At 200 μmol/L for 24 h，resveratrol effectively inhibited the proliferation of A2780 cells，and the cells were blocked in G0/G1 phase，and they were in a significant dose-time dependence（P<0.05）. Western blot results showed that resver－atrol significantly lowered the expression of ILK，β-catenin and Cyclin D1 at protein levels in A2780 cells（P<0.05），with a concentra－tion-time dependence（P<0.05）. Conclusion：Resvertrol could inhibit the proliferation of ovarian cancer cells by inhibiting ILK/β-catenin signaling pathway and reducing the expression of downstream target protein Cyclin D1 in A2780 cells.