Screening and analyzing related genes and miRNA in heart failure caused by idiopathic dilated cardiomyopathy

Abstract:

Objective：To screen potential hub genes and miRNA in the process of heart failure caused by idiopathic dilated cardiomyopathy（IDCM）. Methods：Two gene expression profiling chips from GEO database were included. After eliminating batch effects and normalizing the data，common differentially expressed genes（DEGs） were screened out. Then，gene ontology（GO） analysis and KEGG pathway enrichment analysis were performed. Protein-protein interaction（PPI） network was constructed by STRING online tool. miRNet was used to obtain miRNA targeting DEGs，and miRNA-DEGs network was constructed. And Cytoscape was used to analyze hub genes and miRNA. Results：According to ｜logFC｜ > 0.4 and P < 0.05，a total of 210 DEGs were screened out. These DEGs were mainly enriched in PI3K signaling pathway，MAPK signaling pathway and tumor-related pathways. Hub genes including STAT3，MYC and CCND1 as well as key miRNAs such as miR-34a-5p，miR-17-5p and miR-20a-5p were selected. Conclusion：Based on bioinformatics methods，the hub genes and miRNA in the process of heart failure caused by IDCM were screened and analyzed，a total of 210 differentially expressed genes and 7 functional modules were screened，and 3 key miRNAs were analyzed，which provided ideas for further research on the mechanism and therapeutic targets of IDCM heart failure.