Exploration on plasma biomarkers of Acinetobacter baumannii infection based on proteomics techniques

Abstract:

Objective：To find the plasma biomarkers of Acinetobacter baumannii（A. baumannii） infection based on proteomics tech－niques. Methods：Plasma of patients with blood stream infection was collected，and was divided into A.baumannii infection group（n=16），other G- bacteriainfection group（8 cases of K. pneumoniae infection and 8 cases of E. coli infection），and plasma of other 16 healthy people（control group） was collected. The plasma proteins of the above subjects were analyzed by mass spectrometry and bioinformatics to find the specific proteins related to A. baumannii infection. Results：There were 13 proteins in A. baumannii infection which were different from other G- bacteria infections，52 proteins were different from health controls，and 3 differential proteins in the same time（CNDP1，IGLL5，PEDF，all P<0.05）. Data from the GO analysis showed that most differential proteins were in the extracellular region，which were mostly related to coagulation，inflammation and immune response，while KEGG analysis showed that most proteins were involved in the complement and clotting cascade pathway. Conclusion：Changes in plasma proteome of A. baumannii infection were closely related to inflammation，coagulation and immune response. CNDP1，IGLL5 and PEDF may be candidate plasma biomarkers for A. baumannii infection.