Progress in rapid detection of nucleic acid molecules by integrated paper-based microfluidics

Abstract:

Nucleic acid is of great importance to disease diagnosis because of its rich genetic information. Traditional nucleic acid detection technology is limited by the limitation of time consuming and large equipment， which seriously restricts its application in large-scale and sudden disease detection. Paper-based microfluidic technology has the advantages of portability and self-actuation， providing the possibility for rapid and accurate detection of nucleic acid molecules after integrating isothermal amplification technology. Based on the design of paper-based microfluidic technology，this review has summarized the research progress of paper-based microfluidic technology in the detection of nucleic acid molecules，and analyzed the interference factors that affect the sensitivity and stability of the detection， providing an important reference for promoting the clinical transformation of paper-based microfluidic technology.