Current status and progress of nucleic acid point-of-care testing technology based on CRISPR/Cas system

Abstract:

In the context of the global epidemic， nucleic acid detection under on-site conditions plays a more and more important role in epidemic prevention and control， which makes the development of nucleic acid point-of-care testing（POCT） tools facing unprecedented demand. The application of clustered regularly interspaced palindromic repeats-associated nuclease（CRISPR/Cas） to nucleic acid POCT has been developed rapidly and achieved good results in recent years. In the epidemic control areas，customs and disaster areas and other special sites，combining isothermal amplification techniques with CRISPR and being integrated into small handheld devices，these technologies are easier to use and non-professionals can quickly and accurately detect the nucleic acid source of infection. This review first briefly introduces the CRISPR/Cas system and different effector proteins， reviews the different application strategies in nucleic acid POCT based on the specific recognition and cleavage characteristics of CRISPR/Cas system， and finally discusses the challenges and future research prospects of CRISPR/Cas in nucleic acid POCT.