Abstract:Objective: To investigate the effect of microRNA-132-5p (miR-132-5p) targeting S100 calcium-binding protein A9 (S100A9) on the apoptosis and inflammatory of mesangial cells induced by high glucose. Methods: Human mesangial cells were cultured in DMEM normal glucose medium as control group, and cultured in DMEM high glucose medium as high glucose group.miR-NC, miR-132-5p, anti-miR-NC, anti-miR-132-5p were transfected into normal cultured mesangial cells and recorded as miR-NC group, miR-132-5p group, anti-miR-NC group, anti-miR-132-5p group; miR-NC, miR-132-5p, si-NC, si-S100A9 were transfected into mesangial cells and cultured in a high glucose medium, and recorded as high glucose+miR-NC group, high glucose+miR-132-5p group, high glucose+si-NC group, high glucose+si-S100A9 group; miR-132-5p was transfected with pcDNA and pcDNA-S100A9 into mesangial cells and cultured with high glucose medium, and recorded as high glucose+miR-132-5p pcDNA group, high glucose+miR-132-5p+pcDNA-S100A9 group. Real-time quantitative PCR (RT-qPCR) was used to detect miR-132-5p and S100A9 mRNA expressions. Western blot was used to detect S100A9, Bcl-2 associated X protein (Bax), and B cells lymphoma/leu-kemia-2 protein (Bcl-2) expression; dual luciferase assay was used to verify the targeting relationship between miR-132-5p and S100A9; flow cytometry was used to detect apoptosis; enzyme-linked immu-nosorbent assay (ELISA) detected the levels of tumor necrosis factor-α (TNF-α), interleukin (IL) -8 and IL-6. Results: The expression of miR-132-5p in mesangial cells induced by high glucose was decreased, and the expression of S100A9 was increased (P<0.05).miR-132-5p targetedS100A9, overexpressedmiR-132-5porinhibited S100A9 expression. Apoptosis was reduced in high glucose-induced mesangial cells, Bax expression was reduced, Bcl-2 expression was increased, and TNF-α, IL-8, IL-6 levels were decreased (P<0.05). S100A9 overexpression reversed the inhibitory effect of miR-132-5p overexpression on apoptosis and inflammatory of mesangial cells induced by high glucose. Conclusion: Overexpression of miR-132-5p can inhibit apoptosis and inflammatory of high glucose-induced mesangial cells by down-regulating S100A9.
