Abstract:Objective: To explore the function of RNA-binding protein ABCF1 in the development of human acute myeloid leukemia (AML). Methods: The clinically diagnosed AML patients were enrolled in the experimental group, and normal people in the control group. The expression level of ABCF1 mRNA was respectively detected by RT-qPCR in peripheral blood mononuclear cells (PBMCs) of normal people and bone marrow nucleated cells of AML patients.Human monocytic leukemia cell line THP-1 was infected with two shRNA lentivirus particles targeting ABCF1.The proliferation of THP-1 cells was detected by CCK-8 analysis, and apoptosis and cell cycle were detected by PI and Annexin V staining combined with flow cytometry.RNA pull-down test was carried out by using ss-m6A and ss-A probes, and the binding ability of ABCF1 and m6A was analyzed. Results: The expression of ABCF1 in AML patients in experimental group was significantly higher than that in control group (P<0.01). ABCF1 knock-down significantly promoted apoptosis (P<0.05), and suppressed cell proliferation (P<0.001), as well as affected the cell cycle of THP-1 cells. Conclusion: ABCF1 plays an important role of proto-oncogene in THP-1 cells derived from AML cells and regulates the physiological functions of THP-1 cells.
