Correlation between bone morphogenetic protein 2 gene polymorphism and coronary artery calcification

Abstract:

Objective To investigate the distribution of bone metabolic-related gene polymorphism，such as bone morphogenetic protein 2（BMP2），in the Chongqing Han population and its correlation with coronary artery calcification（CAC）.Methods Clinical data and blood samples were collected from patients with coronary angiography in the Department of Cardiology，The First Affiliated Hospital of Chongqing Medical University from March 2019 to May 2019. The detection of BMP2 rs961253，rs235768，rs1049007；OPG rs2073617，rs2073618 and S100A12 rs2916191 gene were taken into direct sequencing. Serum levels of BMP2，OPG and S100A12 were detected by ELISA.Results There were 167 cases in CAC group，with age of （67.13±9.64） years，and 93 cases in control group，with age of （60.46±9.89） years. Patients with hypertension，diabetes and obesity were more likely to develop CAC. The male morbidity，apolipoprotein A1，C-reactive protein，white blood cell count，serum BMP2，S100A12 and OPG levels in CAC group were significantly higher than those in control group（P<0.05）. The genotype frequency and allele frequency distribution of BMP2 rs961253 were significantly different between CAC group and control group（P<0.05）. In CAC group，the serum BMP2 level of BMP2 TT genotype was significantly higher than that of non TT genotype（P<0.05）. After adjusting gender，age，body mass index，hypertension and diabetes，multivariate stepwise logistic regression analysis found that people with BMP2 rs961253 TT genotype had higher risk of CAC.Conclusion The serum level of BMP2 can be regarded as one of the prediction indicators for CAC. BMP2 rs961253 gene polymorphism is significantly associated with CAC，and BMP2 rs961253 T allele or TT genotype is a high risk factor for CAC.