Expression changes and its significance of SMYD2 in cervical cancer

Abstract:

Objective To explore the role of SET and MYND domain-containing protein 2（SMYD2） in the glucose metabolism of cervical cancer cell，and further study whether it is related to cervical cancer proliferation.Methods Gene expression profiling interactive analysis（GEPIA） was used to analyze the expression profile and prognosis of SMYD family members in cervical cancer，and survival differences were compared by log-rank test. The role of SMYD2 in glucose metabolism in cervical cancer was studied by shRNA knockdown or overexpression of SMYD2.Results The expression of SMYD2 in cervical cancer tissues was not related to age，gender and the degree of tissue differentiation，but was related to clinical stage（F=4.520， P=0.004）. The high expression of SMYD2 predicted poor overall survival. Bioinformatics analysis revealed that SMYD2 played a regulatory role in regulating the Warburg effect in cervical cancer. The deletion of SMYD2 inhibited aerobic glycolysis of cervical cancer cells by functional loss experiments，which showed decreased glucose uptake，lactic acid production and extracellular acidification. SMYD2 overexpression promotes glycolysis metabolism of cervical cancer cells. In addition， SMYD2 was essential for the growth of cervical cancer cells， and this carcinogenic activity was mainly dependent on glycolysis.Conclusion SMYD2 is overexpressed in cervical cancer cells and plays an important role in tumor metabolism， which can promote the proliferation of cervical cancer cells by regulating aerobic glycolysis.