Danggui Shaoyao Powder improves inflammatory response in rats with nonalcoholic fatty liver disease induced by high-fat diet by regulating the Sirt1/NF-κB signaling pathway

Abstract:

Objective To investigate the mechanism of action of Danggui Shaoyao Powder in the treatment of inflammatory response in rats with nonalcoholic fatty liver disease（NAFLD） induced by high-fat diet（HFD）.Methods Rats were randomly divided into normal group，HFD group， avastatin group，Danggui Shaoyao Powder low-dose group，and Danggui Shaoyao Powder high-dose group. The rats were given HFD（containing 59.3% fat） for 6 weeks，while the rats in the normal group were given normal diet. Since week 5，rats in the avastatin group were given 25 mg/kg of Avastatin by gavage for 2 weeks， while rats in the low-dose Danggui Shaoyao powder group were given 12.9 g/kg of Danggui Shaoyao powder by gavage for 2 weeks， and rats in the high-dose Danggui Shaoyao powder group were given 25.8 g/kg of Danggui Shaoyao powder by gavage for 2 weeks，and the serum levels of alanine aminotransferase（ALT），aspartate aminotransferase（AST），triglyceride（TG），cholesterol（TC），and cytokines were measured. Meanwhile，the expression levels of the sirtuin 1（Sirt1）/nuclear factor kappa-B（NF-κB） signaling pathway proteins in rat liver were also measured.Results Compared with the model group，Danggui Shaoyao Powder significantly reduced the serum levels of ALT，AST，TG，TC，and cytokines and improved the pathological changes of the liver. In addition，it significantly increased the expression of Sirt1 and inhibited the expression of NF-κB in rat liver.Conclusion Danggui Shaoyao Powder exerts a therapeutic effect on rats with NAFLD induced by HFD through the Sirt1/NF-κB pathway.