Carvacrol alleviates oxidative stress damage in rats with diabetic nephropathy via the SIRT1/FOXO1 pathway

Abstract:

Objective To investigate the mechanism of carvacrol alleviating oxidative stress damage in rats with diabetic nephropathy （DN） via the SIRT1/FOXO1 pathway. Methods A rat model of DN was established by high-fat diet and intraperitoneal injection of streptozotocin，and then DN rats were divided into control group，model group，L-XQF group（5 mg/kg），M-XQF group（10 mg/kg），and L-XQF group（20 mg/kg）. Fasting blood glucose（FBG），blood urea nitrogen（BUN），serum creatinine（SCr），and urinary microprotein （U-mAlb） were measured. After the rats were sacrificed and renal tissue samples were collected，HE staining and Masson staining were used to observe the pathological changes and fibrosis of renal tissue；ELISA was used to measure the levels of malondialdehyde （MDA），superoxide dismutase（SOD），and glutathione peroxidase（GSH-Px） in renal tissue； Western blot was used to measure the expression levels of SIRT1/FOXO1 pathway-related proteins. Results Carvacrol reduced the levels of FBG，BUN，SCr，U-mAlb，and MDA，improved the pathological injury and fibrosis of renal tissue，and increased the content of SOD and GSH-Px and the levels of SIRT1 and FOXO1 proteins. Conclusion Carvacrol may inhibit oxidative stress in renal tissue of DN rats via the SIRT1/FOXO1 pathway，thereby alleviating renal injury and improving renal fibrosis.