Abstract:Objective: To explore the key techniques and molecular mechanism of the treatment of allergic rhinitis (AR) by nasal mound and inferior turbinate cutting therapy. Methods: From March 2, 2019 to May 30, 2020, the clinical data of 85 patients with AR in Hospital of Chengdu University of Traditional Chinese Medicine were collected, including 32 males and 53 females, aged 35.74±10.24. A visual analogue scale (VAS) score was used to compare the efficacy of different cutting sites, cutting directions and cutting depth at 1 week, 4, 8 and 12 weeks after treatment. The levels of interferon-gamma (IFN-γ), immunoglobulin E (IgE) and inter-leukin-4 (IL-4) in serum of patients before and after treatment were further detected by ELISA. Eosinophils and mastocyte of pa-tients before and after treatment were detected by HE staining and toluidine blue staining. The expression levels of Calcitonin gene related peptide (CGRP) and substance P (SP) were detected by Western blot and qPCR. Statistical analysis was performed with SPSS 22.0. Results: The curative effect of nasal mound combined with inferior turbinate was significantly better than that of simple nasal mound turbinate and simple inferior turbinate (P<0.05). There was no significant difference in efficacy among the three directions of nasal mound turbinate. The results of ELISA showed that the levels of IFN-γ (t=6.069, P<0.001) in AR patients after treatment were significantly increased, IgE (t=6.954, P<0.001) and IL-4 (t=12.060, P<0.001) were evidently decreased. HE staining and toluidine blue staining indicated a significant decrease in eosinophil infiltration and mastocyte activity in the treated patients. Western blot experiment showed that CGRP (t=11.130, P=0.008) and SP (t=11.930, P=0.007) decreased significantly after treatment in AR patients compared with those before treatment. The qPCR experiment showed that CGRP (t=11.640, P=0.007) and SP (t=7.104, P=0.002) decreased significantly after treatment in AR patients compared with those before treatment. Conclusion: The site and depth of the cutting are the key technical parameters for the therapy of nasal mound and inferior turbinate cutting, which can reduce the inflammation of nasal mucosa, and its molecular mechanism maybe reduced expression of CGRP and SP.
