Screening of potential molecular markers in cervical cancer cells by ultra-high-speed cell selection platform combined with cDNA microarray technology

Abstract:

Objective: To screen potential molecular markers of cervical cancer cells by ultra-high speed cell sorting platform combined with cDNA microarray technology. Methods: In this paper, the MoFlo XDP-type ultra-high-speed cell selection platform was used to purify cervical cancer cells expressing CD38 and not expressing CD38 on the cell membrane surface. RNAlater technology was used to obtain the RNA required for cDNA microarray experiments, and then by gene chip analysis was made. Results: The MoFlo XDP-type ultra-high-speed cell selection platform can be used to obtain cervical cancer cells with a CD38-positive expression of 99.0% purity. Conclusion: Through the analysis of cDNA microarray, potential molecular markers of cervical cancer cells such as RORA, PLIN4, AUTS2, and IFITM1 have been found, which provides a new technical method for the research of cervical cancer.