Proteasome activator subunit 1/2 promotes the proliferation and invasion of endometrial carcinoma cells by inhibiting the expression of cyclin-dependent kinase 15

Abstract:

Objective To investigate the expression level of proteasome activator subunit 1/2（PSME1/2） in endometrial carcinoma and its effect on the proliferation and invasion of endometrial carcinoma cells.Methods The expression levels of PSME1/2 and cyclin-dependent kinase 15（CDK15） were measured in endometrial cell lines and primary cultured cells，and knock-down strains were used to analyze the correlation between PSME1/2 and CDK15 and the effect of PSME1/2 on tumor proliferation and invasion. The expression levels of PSME1/2 and CDK15 were compared between endometrial carcinoma tissue and paracancerous tissue，and the influence of PSME1/2 and CDK15 on the prognosis of patients with endometrial carcinoma was analyzed.Results Compared with normal human endometrial cell lines，human endometrial adenocarcinoma HEC1B cells and primary cultured cells had high mRNA and protein expression levels of PMSE1/2 and a reduction in the protein expression of CDK15. Compared with the control group and the double knock-down group，there was an increase in the protein expression level of CDK15 in the HEC1B line with PSME1/2 knock-down，suggesting that CDK15 might be inhibited by PSME1/2. There was a high expression level of PMSE1/2 and a low expression level of CDK15 in endometrial carcinoma. There were no significant differences in age，postoperative diagnosis and typing，degree of tumor differentiation，and postoperative stage between the high PSME1/2 expression group and the low PSME1/2 expression group（P>0.05）. There was no significant correlation between the expression of PSME1/2 and CDK15 and the poor prognosis of patients with endometrial carcinoma（P>0.05）.Conclusion PSME1/2 can inhibit the expression of CDK15 and promote the proliferation and invasion of endometrial carcinoma cells，and PSME1/2 can also promote endometrial carcinoma.