The expression of MTAP in prostate cancer and its effect on immune cells

Abstract:

Objective To explore the expression of Methylthioadenosine phosphorylase（MTAP） in different clinical stages of prostate cancer and its correlation with immune cells.Methods The expression profile of MTAP was obtained from TCGA and GTEx databases，and pancarcinoma was analyzed by bioinformatics method. Immunohistochemistry verified the conclusions above and explored the correlation between MTAP and clinicopathological features. The expression levels of MTAP in different prostate cancer cell lines were detected by RT-qPCR and Western blot，and the correlation between MTAP and the degree of malignancy was analyzed. The correlatoin between MTAP and immune cells in prostate cancer was analyzed by bioinformatics and verified by immunohistochemistry.Results In prostate cancer，the expression of MTAP increased at early stage and then decreased gradually，and was negatively correlated with T stage and Gleason score（r_s=-0.576，P=0.000，r_s=-0.284，P=0.020）. MTAP was correlated with a variety of immune cells，among which MTAP was positively correlated with CD4⁺T cells（r=0.643，P=0.000），was significantly negatively correlated with NK CD56^bright cells（r=-0.570，P=0.000）.Conclusion In prostate cancer，the expression of MTAP increased at the early stage and then decreased gradually，which was negatively correlated with T stage and Gleason score. MTAP can regulate the infiltration and function of immune cells，and is a potential biomarker and immunotherapy target.