Abstract:Objective:To investigate the correlation between the distribution of three single nucleotide polymorphisms (SNPs) of Klotho(KL) G-395A,F352V and C370S in the Klotho(KL) gene and metabolic syndrome(MS) in the elderly Han population. Methods：Totally 329 patients (aged≥60) with MS and 321 healthy people were enrolled. The single allele specific prime PCR (SASP-PCR) technique was used to detect three sites of SNPs and the distribution frequencies of allelic genotypes of the KL gene in order to analyze their relationship with MS in the aged. 〖WTB4X〗Results〖WTBZ〗: The frequency of allele AA genotype of KL G-395A in MS group was higher than that in the control group but without any significant difference (P＞0.05). According to sex stratification analysis, no significant difference in the frequency of allele AA was found either in the female subgroup or the male subgroup between the MS group and the control subgroup(P＞0.05).The frequency of allele VV genotype of KL F352V in MS group was higher than that in the control group (P＝0.053), but no significant difference of the frequency of allele VV of KL F352V was found either in the female or male subgroup between MS group and the control group according to sex stratification analysis. The frequency of allele CS genotype of KL C370S SNP in MS group was lower than that in the control group(P＜0.05). According to sex stratification analysis, the frequency of allele CS and SS in the MS male subgroup was obviously lower than that in the control subgroup(P＜0.05). Conclusion：Correlation between VV mutant genotype of KL F352V SNP and metabolic syndrome in the elderly people may be existed and the males who carried mutant genotypes of KL C370S SNP have a low risk in suffering from MS.

Abstract:Objective:To study the cancer testis (CT) gene expression in medulloblastoma of the Chinese population.Methods:Totally 25 medulloblastoma cases were collected. RT-PCR and immunohistochemical methods were used to detect medulloblastoma CT gene and protein expression respectively, combined with genotyping analysis. Results:Tumor types were statistically different between each other in non X-CT antigens(P<0.05). CT gene was expressed in at least 1 case among 25 cases. GAGE was the most commonly expressed CT gene,existing in 17 cases (68%). CT-X gene co expression was found in 19/20 cases (95%) while non X-CT gene co expression in 1/18 cases (5.6%). Among all cases, monoclonal antibody CT 7-33 (MAGEC1) was the most positive protein staining. The CT gene mRNA expression and clinical parameters (such as age and clinical outcome) were statistically analyzed, displaying no significant correlation.Conclusion：At the molecular level, CT antigen exists in at least one CT gene. Many CT genes have mRNA expression,but few have protein expression.

Abstract:Objective:To evaluate the proliferative and apoptosis effects of Paclitaxel loaded microbubbles plus ultrasound on ovarian cancer A2780/DDP cell strain. Methods:A2780/DDP cell strain was randomly divided into five groups:control group, Paclitaxel group,Paclitaxel plus ultrasound irradiation group,microbubbles loaded with Paclitaxel group and microbubbles loaded with Paclitaxel plus ultrasound irradiation group. The proliferative activity of human ovarian cacinoma cell line A2780/DDP was measured by MTT assay at 24, 48 h and 72 h after the treatment and the apoptosis was analyzed by Hochst 33258 and TUNEL staining methods.Results:Resutls from MTT showed that the inhibitory rate of Paclitaxel loaded microbubbles plus ultrasound irradition group were (37.20±2.01)%,(51.60±2.10)% and(57.47±2.85)% respectively at 24, 48 h and 72 h after the treatment, significantly higher than those in the other groups (P<0.05). Results from Hochst 33258 and TUNEL staining indicated that the apoptosis rate was (46.10±4.15)% in microbubbles loaded with Paclitaxel plus ultrasound irradiation group at 24 h after the treatment,obviously higher than those in the other groups (P<0.05). Conclusion:Paclitaxel loaded microbubbles plus ultrasound irradiation can suppress the growth and induce the apoptosis of A2780/DDP cell.

Abstract:Objective:To investigate the effect of siRNA-mediated nuclear factor with BRCT domains protein 1(NFBD1)depletion on chemosensitivity in nasopharyngeal carcinoma cells. Methods：Using siRNA to inhibit expression of NFBD1 in nasopharyngeal carcinoma cell lines HNE-1(poorly differentiated squamous-cell carcinoma) and CNE-1(goodly differentiated squamous-cell carcinoma), RT-PCR and Western bolt analysis were used to verify the inhibitory effect of siRNA against NFBD1. MTT assay was used to determine the effect of NFBD1 depletion on the chemosensitity of nasopharyngeal carcinoma cells. Results：siRNA could specifically and efficiently deplete NFBD1 in nasopharyngeal carcinoma cells. Survival rate in the NFBD1 siRNA group was significantly degraded,compared with that in blank group and the control siRNA group. Conclusion： SiRNA-mediated NFBD1 depletion can significantly enhance the chemosensitivity of nasopharyngeal carcinoma cells to chemotherapeutic agents such as cisdiamminedichloroplatinum(CDDP).

Abstract:Objective:To examine the changes of the renal tissue structure and the expression changes of hepatocyte growth factor(HGF) in the renal tissue of neonatal rats exposed to hyperoxia. Methods: Neonatal rat lung injury model was induced by exposing to hyperoxia.Seventy-two neonatal sprague-dawley(SD)rats were randomly divided into groupⅠ(n=36) and groupⅡ(n=36) within 12 h after birth. The rats in group Ⅰwere exposed to normal air while those in groupⅡ to hyperoxia. Eight rats in each group were killed on 3th,7th,14th d after birth to make the samples. The changes of the renal tissue structure were examined by hematoxylin-eosin (HE) staining method;the protein expressions of HGF in the renal tissue were detected by immunohistochemical method;the mRNA expression intensities of HGF were measured by reverse transcription polymerase chain reaction (RT-PCR). Results: ①Mesangial cell proliferation,renal tubular epithelial cells swelling and inflammatory cells infiltration were observed in group Ⅱcompared with those in groupⅠ.The pathological changes became more and more obvious with the passage of time.②The protein expression levels of HGF were enhanced on 3th d in groupⅡ compared with those in groupⅠ (P<0.05),with more enhanced effect on 7th and on 14th d(P<0.05). However,the enhanced tendency on 14th d was weaker than that on 7th d. ③HGF mRNA expression intensities were similar to the changing tendency of their protein expression levels. Conclusion:The expression levels of HGF are enhanced in hyperoxia induced renal tissue injury.

Abstract:Objective:To study the correlation among circulating estrone (E1), 2-hydroxyestrone(2-OHE1), 16α-hydroxyestrone(16α-OHE1), the ratio of 2-hydroxyestrone to 16α hydroxyestrone(2-OHE1∶16α-OHE1) and breast cancer in postmenopausal women. Methods:Serum levels of E1,2-OHE1,16α-OHE1 were measured by ELISA method in 40 cases of breast cancer,21 cases of breast fibroadenoma and 19 cases of mastopathy in postmenopausal women; the ratio of 2-OHE1 to 16α-OHE1 was calculated. Results:There were no significant difference in circulating levels of E1,16α-OHE1 and the ratio of 2-OHE1 to 16α-OHE1 in three groups (P＞0.05). The levels of 2-OHE1 were significantly different in three groups（P<0.05）; it’s level in breast cancer group was significantly lower than that in mastopathy group（P<0.05）. Circulating levels of E1,2-OHE1,16α-OHE1 and the ratios of 2-OHE1 to 16α-OHE1 were not associated with ER, HER2, axillary lymph node status or tumor size (P＞0.05). Breast cancer risk was not associated with the ratio of 2-OHE1 to 16α-OHE1(Odds ratio was 0.828, 95% confidence interval was 0.425~1.611, P＞0.05)，but it was negatively associated with the level of 2-OHE1(Odds ratio was 0.418, 95% confidence interval was 0.180~0.971, P<0.05).Conclusion：The results suggest that the level of 2-OHE1 is negatively associated with breast cancer risk, and do not support that the ratio of 2-OHE1 to 16α-OHE1 predicts breast cancer risk.

Abstract:Objective:To detect the expressions,distribution and the significances of aquaporin 1（AQP1）,aquaporin 2（AQP2）and aquaporin 3（AQP3）in normal bladder tissues and bladder tumor tissues. Methods：The expression and distribution of AQP1、AQP2 and AQP3 were detected by S-P immunohistochemistry in 25 cases of bladder tumor tissues and normal bladder tissues of 25 cases. Results：The AQP1was sporadically expressed in the vascular endothelial cells of normal bladder tissues. The majority expression of AQP1 could be detected in the vascular endothelial cells of tumor cells. The expression of AQP1 was remarkably higher in tumor tissues than in normal bladder tissues. Sporadic expression of AQP2 could be detected in intercellular substance in both bladder tumor tissues and normal bladder tissues. The expression of AQP2 in both tumor tissues and normal bladder tissues had no significant difference.the expression of AQP3 was found mainly in the celluar membrane and periplasm of the mucous membrane in both bladder tumor tissues and normal bladder tissues. The expression of AQP3 was significantly higher in tumor tissues than in normal bladder tissues. Conclusion：The expression of AQP1 and AQP3 were highter in bladder tumor tissues than those in normal bladder tissues. It suggested that AQP1 and AQP3 might related with the formation and development of bladder tumor. But the mechanism need to be further studied.

Abstract:Objective:To investigate the correlations of high mobility group protein box-1 (HMGB1) expression with vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in hepatocellular carcinoma (HCC) and its clinical significance. Methods:Expressions of HMGB1, VEGF and CD34 were detected with immunohistochemistry in 37 specimens of HCC tissues and the corresponding adjacent tumor tissues, and 10 cases of normal hepatic tissues. The MVD was calculated using CD34 antibody as an endothelial marker. Western blot was performed in 10 HCC tissues and the corresponding adjacent tumor tissues, and 5 normal hepatic tissues. Results:Expression of HMGB1 appeared at a high level compared to adjacent tumor tissues and normal hepatic tissues. HMGB1 expression was significantly correlated with the tumor size and the portal venous invasion. HMGB1 expression was positively correlated with VEGF expression and MVD in HCC（r=0.544, P=0.001；r=0.672，P＜0.05）. Conclusion:Upregulation of HMGB1 and VEGF are involved in the angiogenesis of HCC, and HMGB1 may be a therapeutic target for angiogenesis in HCC.

Abstract:Objective:To investigate the radiosensitive effect of all trans retinoic acid(ATRA) on lung cancer cell line A549 cultured in vitro and its possible mechanism. Methods: The A549 cells in the logarithmic growth phase were divided into four groups: control group, ATRA group, radiation group, combination group(ATRA combined with radiation). MTT assay was used to detect the growth inhibition rate and to further filter out the best experimental concentration of ATRA. The radiosensitive effect was detected by clone formation test. The cell cycle and apoptosis was measured by flow cytometry. The expression of VEGF mRNA was detected by RT-PCR. The VEGF values in the cell supernatant were detected by ELISA. Results: The optimal dose of ATRA was 10 μmol/L. ATRA can enhance the radiosensitivity of A549 cells. The proportion of G0/G1 phase in ATRA group was significantly increased compared with that in control group（P<0.05）;statistic differences in apoptosis rate were observed between ATRA group and control group（P<0.05）. The expressions of VEGF mRNA were significantly reduced in combination group compared with those in radiation group（P<0.05）. The VEGF levels in the supernatant of combination group were significantly decreased compared with those in radiation group（P<0.05）. Conclusion: All-trans retinoic acid can increase the radiosensitivity of A549 cells. The mechanism may be related with the direct inhibition of ATRA on A549 cells, redistribution of the inducing cell cycle, inducement of the apoptosis and down regulation of the expression of VEGF in tumor cells.

Abstract:Objective: To observe the growth inhibition effect of the single clone antibody of epidermal growth factor receptor (EGFR) Cetuximab combined with Paclitaxel (PTX) on human breast cancer cell line MCF-7.Methods: The experiment involved four groups: control group, Cetuximab group,PTX group and combination group. The inhibitory rates of Cetuximab,PTX and different combinations of Cetuximab and PTX on MCF-7 cells were examined by MTT assay and the apoptosis rate and cell cycle distribution were analyzed by flow cytometry. The immunofluorescence and RT-PCR were used to evaluate the EGFR protein and mRNA expression in control group and PTX group. Results:PTX can exert inhibitory effect on MCF-7 cells in a dose dependent manner based on the results from MTT assay. MTT assay showed that different combination schemes exerted different inhibitory effects; the synergistic effect was seen in the scheme of MCF-7 cells first treated with PTX and then Cetuximab. The apoptosis rate of the other three groups was increased compared with that of control group and the effect was more evident in combination group than in single agent groups (P<0.05).The EGFR protein and mRNA expressions reduced in PTX group compared with those in control group (P<0.05).Conclusion：Human breast cancer cell line MCF-7 was sensitive to PTX and PTX can exert inhibitory effect on MCF-7 cells in a dose dependent manner. The combination of Cetuximab and PTX can increase the apoptosis and decrease the proliferation of breast cancer cell line MCF-7 with a synergistic action.

Abstract:Objective:To observe the inhibitory effect of Curcumin on human alveolar rhabdomyosarcoma cell line PLA-802 in vitro, to detect the expressions of TGF-beta1 and Smad 4, and to explore its potential mechanisms. Methods：Human alveolar rhabdomyosarcoma cell line PLA-802 was cultured and treated with Curcumin at different concentration and at different time points. The effect of Curcumin on PLA-802 cell proliferation was studied by means of MTT; the cell cycle was detected with flow cytometry; RT-PCR and western blot were used to detect the expressions of TGF-β1 and Smad 4 at mRNA and protein levels. Results：The viability of PLA-802 cells treated with Curcumin was obviously decreased (P<0.05). Analysis of the cell cycle revealed that Curcumin induced a significant decrease in cells in the S phase and an increase in cells in the G1 phase (P<0.05). Furthermore, the expressions of TGF-β1 and its downstream factor Smad 4 at mRNA and protein levels were inhibited by Curcumin in a concentration and time dependent manner (P<0.05). Conclusion:Curcumin perhaps plays its inhibitory effects on PLA-802 cells through inhibiting the the activity of TGF-β1 signaling pathway, which will provide an important therapeutic potential for treating human aleovar rhabdomyosarcoma.

Abstract:Objective:To evaluate the effects of down regulating hepatoma-derived growth factor (HDGF) of glioma on the proliferation and apoptosis of U373 cells.Methods:siRNA targeting human HDGF gene was designed and transfected transiently into U373 cells by lipofectamine. RT-PCR and Western blot were used to detect the changes in expressions of HDGF gene and protein as well as to evaluate the efficacy of RNA interference. Flow cytometric detection and MTT method were used to detect cell apoptosis and proliferation. Results:RT-PCR and Western blot results showed that the expressions of HDGF gene and protein declined obviously in U373 cells at 48 hours after the transfection. Meanwhile,the MTT results indicated that the proliferation in vitro was remarkably decreased and the apoptosis rate was significantly increased in U373 cells compared with those in blank and negative control group after down regulating HDGF expressions (P<0.01 andP<0.05). Conclusion:HDGF plays a conductive role in the development of glioma,which may become a new molecular marker in tumor diagnosis and treatment.

Abstract:Objective:To discuss the possible mechanism of lipopolysaccharide(LPS) pretreatment in alleviating acute pancreatitis (AP) in rats by detecting the changes in expressions of interleukin-1 receptor associated kinase 4(IRAK-4) in the pancreas. Methods: Sixty three male Sprague-Dawley rats were divided randomly into three groups:sham group, acute pancreatitis group (AP group) and LPS pretreatment group(LPS group). LPS was injected intraperitoneally to the rats in LPS group while equal amount of normal saline was injected to the rats in the other two groups. Samples were achieved at 3 h after the model was built in sham group while at 3,6,12 h and 24 h after modeling in the other two groups. The morphology of pancreatic tissue was analyzed by light microscopy respectively. The expressions of IRAK-4 gene and protein level of pancreas were determined by reverse RT-PCR and western blot, the activities of NF-κB were detected by immunohistochemical method and the serum TNF-α level was estimated by ELISA.Results: The pathological damage was most serious in AP group, followed by LPS group and sham group. The expressions of IRAK-4 mRNA and protein in AP group reached the peak at 12 h then decreased. The expressions of IRAK-4 mRNA and protein were obviously lower in LPS group and were maintained a lower level (P>0.05) compared with those in AP group after the pretreatment (P<0.01).The activities of NF-κB and the level of serum TNF-α were obviously lower in LPS group compared with those in AP group at all time periods (P<0.01),both group reaching the peak simultaneously at 12 h(P<0.01). Conclusion: Down-regulating the expression of IRAK-4, NF-κB and TNF-α may be one of the protective mechanisms of LPS pretreatment for AP.

Abstract:Objective:To investigate the antiangiogenic ability and mechanism of Vinorelbine in vitro and in vivo. Methods: In vitro, MTT colorimetric assay was used to observe the effect of Vinorelbine on the proliferation of human umbilical vein endothelial cell (HUVEC) and human pulmonary adenocarcinoma A549 cells. Transwell cabin test and out body canaliculization test were used to observe the impact of Vinorelbine on cell migration and capillary like tube formation ability, and the apoptosis rate of HUVEC was calculated by flow cytometry (FCM). In vivo, the chicken chorioallantoic membrane (CAM) model was used to check whether the neovascularization of CAM could be suppressed. Results: Low dose Vinorelbine (0.1～1.0 ng/ml) could inhibit the proliferation of A549 and HUVEC after 48 h treatment, and the inhibition had significant differences (P=0.000). The number of migrating HUVEC was decreased as compared with that in the control group (P<0.05,F=72.979,P=0.000), and the inhibition rate of migration was between 20/56%~40.40% when treated with 0.1~0.8 ng/ml Vinorelbine for 12 h. The number of tubules was reduced and the lumen lost its integrity as compared with that in the control group (P<0.05,F=75.794,P=0.000), and the inhibition rate of tube formation was between 29.07%~56.83% when treated with 0.1~0.8 ng/ml Vinorelbine for 24 h. When HUVEC was incubated with 0.1~0.8 ng/ml Vinorelbine for 48 h, the apoptosis rate was between 22.30%~37.05%. At the same time, the apoptosis rate was higher than that of 15.60% in the control group (P<0.05); Note: compared with that of the control, P value for 0.1,0.4 ng/ml, and 0.8 ng/ml Vinorelbine was P=0.036, P=0.013, and P=0.012 respectively). We also observed that Vinorelbine, at concentrations of 0.1, 0.4 ng/ml, and 0.8ng/ml, suppressed the neovascularization of CAM in vivo, and the inhibition rate was between 21.05%~42.11%. Moreover, the inhibition rate was enhanced as the drug concentration increased. Conclusion: ① In vitro, low dose Vinorelbine can inhibit the cell proliferation, migration, and tube formation of HUVEC, and has the ability of inducing apoptosis of HUVEC. ② In vivo, low dose Vinorelbine demonstrates antiangiogenic ability of CAM.

Abstract:Objective:To explore the influence of early maternal isolation stress on the epilepsy susceptibility of li-pilocapine and amygdala kindling induced seizures models in infantile rats. Methods:Totally 66 newborn SD rat pups were enrolled and randomly divided into three groups (n=22)：the normal control group, the maternal separating 15 minutes group and the maternal separating 3 hours group. All rats were performed with li-pilocapine and amygdala kindling induced-seizures experiment at 16/18 days after the birth. Results: The latency period of li-pilocapine induced-seizures was obviously shortened and the convulsive threshold of amygdala kindling as well as the stimulating number needed for full kindling were markedly decreased in the maternal separating 3 hours group compared with those in the normal control group at 2～15/17 days after the separation (P<0.05). However, there was no significant difference in the above indicators between the normal control group and the maternal separating 15 minutes group at 2～15/17 days after the separation(P>0.05). Conclusion:The stress of early maternal separating for 3 hours can shorten the latency period of li-pilocapine induced-seizures, decrease the threshold of amygdala kindling and the stimulating number needed for full kindling thus enhance the susceptibility of the two seizure models in infantile rats.

Abstract:Objective:To select the best prescription for Dextromethorphan (DM) chewing gum preparation and to evaluate its quality. Methods: The DM chewing gum preparation was prepared by tablet press method; the formulation was optimized by orthogonal experiments.Results: The best prescription based on the results of orthogonal experiments was that the proportion of gum base,aspartame,menthol and essence was 90%,3%,0.5% and 0.2%,respectively. Conclusion: DM chewing gum preparation can be successfully formulated and its quality can be guaranteed.

Abstract:Objective: To construct the expression vector of human α1-microglobulin (α1M) gene, express α1M protein in Escherichia coli (E.coli), purify recombinant α1M protein and to verify its bioactivity. Methods: The α1M gene sequence was amplified by PCR and α1M protein was expressed in E.coli B834. The recombinant protein was purified by nickel column chromatography, ion-exchange column chromatography and size exclusion chromatography and its bioactivity was verified by ABTS method. Results: The expression vector of the α1M was successfully constructed by PCR and α1M protein was expressed by E.coli B834 in a soluble supernatant way. The α1M protein with 95% purity was acquired successfully by purification. The result of the size exclusion chromatography indicated that α1M protein exhibited as both monomer and dimer in the solution. Additionally, the recombinant protein exerted anti-oxidant activity by ABTS method. Conclusion: α1M could be highly expressed in supernatant solution in E.coli B834 strain and it can be easily purified. The recombinant protein exhibits anti-oxidant activity, laying a solid basis for further structural and functional study of the human protein.

Abstract:Objective:To design a more accurate regression equation by grouping different heights (short,medium,tall).Methods: Totally 206 adult females aged between 18.1 and 35.1 years were enrolled. The body height, tibia length and ulna length were measured by standard anthropometric techniques. The subjects were randomly divided into group 1 (n=103) and group 2 (n=103). General regression equation and grouping regression equation were set up to estimate the height.The differences between the actual and estimated heights were evaluated using the two independent samples t-test.Results:The grouping regression equation using ulna length as the independent variable yielded more accurate results while that using tibia length,ulna length and tibia and ulna length as the independent variable respectively did not have obvious advantages. Conclusion: Results shows that the grouping regression equation using ulna length as the independent variable give more accurate height estimates for those who are too short or too tall.

Abstract:Objective:To investigate the efficacy of mechanical and pharmacological prophylaxis against deep venous thrombosis(DVT) and pulmonary embolism(PE) following blunt trauma. Low-molecular-weight-heparin（LMWH）,intermittent pneumatic compression（IPC）, and LMWH combined with IPC were used.Methods:Totally 329 patients with blunt fractures and joint injuries from April 2008 to March 2010 were divided into 3 groups randomly. In group 1,98 patients received LMWH injection in 24～48 h after the blunt trauma, once a day.In group 2,107 patients received IPC since hospitalization for 6 h every day.In group 3,102 patients received IPC after hospitalization and retarded LMWH as well. All the patients were evaluated for the clinical symptoms of DVT and PE, examined by colour duplex ultrasonography, and the patients with suspected PE completed the spiral CT pulmonary angiography in 24 h.Results:In group 1,11 cases of DVT(11.22%), 3 cases of PE(3.1%), and 8 cases of big or blocking coagulum(8.2%) were found. In group 2, 27 cases of DVT(25.2%), 14 cases of PE(13.1%), and 11 cases of big or blocking coagulum(10.3%) were found. In group 3,8 cases of DVT(7.8%), 2 cases of PE(2.0%), and 1 case of big or the blocking coagulum(1%) were found. The incidences of DVT and PE in group 1 and 3 were lower than those in group 2 (P <0.05).The incidence of big or blocking coagulum in group 3 was lower than those in group 1 and 2 (P<0.05).Conclusion: After the serious blunt fractures and joint injuries, the early use of IPC and retarded use of LMWH for prophylaxis against deep venous thrombosis following blunt trauma is effective, which also can remarkably reduce the incidence of big or blocking coagulum.

Abstract:Objective:To evaluate the efficacy of trans pedicular and trans-vertebral disc osteotomy in the treatment of kyphosis and to discuss its indications.Methods：Twenty-five patients with kyphosis deformity were surgically treated with trans pedicular and trans vertebral disc osteotomy including 15 males and 10 females with the average age of 48.6 years old (from 8 to 69 years old).There were 19 cases of post traumatic kyphosis and 6 cases of congenital kyphosis with scoliosis. The preoperative mean kyphotic Cobb angle was (40.56±11.05)°.According to the Frankel grading system, 1 case was classified as grade C, 1 case as grade D and 23 cases as grade E preoperatively. All the patients had severe thoracolumbar dorsum pain with difficulty of lying flat. There were 2 cases complicated with bladder sphincter dysfunction and 4 cases of congenital kyphosis underwent progressive deformities.Results：All surgeries were finished successfully. The operation time was 3.0～6.0 h (averaged 4.5 h) and the perioperative bleeding was 800～2 500 ml (averaged 1 550 ml). All incisions were healed by the first intention with no infection or deep venous thrombosis being observed. All cases were followed up from 12～28 months (averaged 18 months). No pseudoarthrosis and implant failure were occurred. Preoperative back pain was allievated in all cases. Neurologic improvement was occurred in 1 case from Frankel grade C to grade D after the surgery. The bladder sphincter function were also improved in 2 cases postoperatively.The Cobb angles of kyphosis at one week after the operation and the last follow-up were obviously improved when compared with the preoperative ones, showing significant differences (P<0.05).No obvious correction loss was observed either in coronal or sagittal plane. Conclusion：Trans-pedicular and trans-vertebral disc osteotomy is an effective and safe surgical technique for kyphosis.

Abstract:Objective:To study the X-ray, CT and MRI features of synovial sarcoma and improve the understanding of synovial sarcoma imaging features. Methods: The X-ray, CT and MRI findings were retrospectively reviewed in 17 patients with synovial sarcoma proved by operation and pathology. Results: 13 cases occurred in lower or upper extremities, 3 cases in body, 1 case in left lung. On X and CT imaging, the density of lesion was heterogeneous, with 4 cases demonstrating peripheral mass calcification, 3 cases showing bone destruction, and 4 cases showing bone resorption. On T1WI, three cases were usually homogeneous with signal intensity similar to or slightly higher than that of muscles and on T2WI they presented prominent homogeneous with high signal intensity. On T1WI and T2WI/STIR, 8 cases presented slightly hyperintense “cobble” nodules, with hypointense septa among the nodules. After contrast, the nodules were unenhanced or enhanced slightly, and the septa were markedly enhanced. Conclusion: Synovial sarcoma have some characteristic findings. The characteristics of synovial sarcoma can be better displayed by combing the X-ray, CT and MRI, and the qualitative and differential diagnosis can thus be improved.

Abstract:Objective:To investigate the correlations among serum adiponectin,interleukin-6(IL-6) and intimal-medial thickness(IMT) of the carotid artery in patients with cerebral infarction(CI) and to explore their significances in the development of cerebral infarction.Methods:Ninty-six patients with CI were enrolled as CI group and 50 healthy volunteers as control group.Serum adiponectin and serum IL-6 levels were detected respectively using enzyme-linked immunosorbent assay (ELISA).Carotid artery IMT was measured in both groups and the patients were subdivided into two groups based on the IMT value (greater or smaller than 1.0 mm).Results: There were significant differences in serum adiponectin and IL-6 levels between CI group and control group (P<0.05).The serum adiponectin and IL-6 levels were also significantly different between CI patients with carotid IMT equal to or greater than 1.0 mm and those with IMT smaller than 1.0 mm (P<0.05).In CI group,IMT was positively correlated with serum IL-6 level (R=0.52,P<0.01) but it was negatively correlated with serum adiponectin level (R=-0.848,P<0.01).Conclusion: IMT are increased and the level of serum adiponectin is reduced in patients with CI.The levels of IL-6,serum adiponectin and IMT in patients with CI are obviously correlated.

Abstract:Objective:To investigate the changes of blood plasma endothelin-1(ET-1), thromboxane A2(TXA2), prostacyclinI2(PGI2) and TXA2/PGI2(T/P) as well as their relationship with vascular lesions in patients with ateriosclerosis obliterans(ASO) before and after interventional therapy. Methods:Totally 42 cases with ASO were divideed into group A, B and C based on transatlantic inter society consensus(TASC). The levels of ET-1, TXA2 ,PGI2in plasma in all cases were measured by method of enzyme-linked immunosorbent assay (ELISA) before and after interventional treatment, then the value of T/P were calculated. Results: The levels of ET-1, T/P were increased in all groups after interventional therapy compared with those before the operation with statistical differences(F=44.34, P<0.01; F=109.09, P<0.01). The level of ET-1 in group B and C before and after the operation was significant higher than in group A with statistical differences(P<0.05). Statistical differences was observed in the value of T/P between group A, B and C(P<0.05). The value of T/P was increased as the vascular lesion become more and more severe. Negative correlation was obseved between postoperative T/P value and postoperative PGI2 value (r=-0.638, P<0.01) while no correlation was found between postoperative T/P value and postoperative TXA2 value(r=0.099，P>0.05). Conclusion: The levels of ET-1 and T/P in blood plasma are incresed in patients with ASO after all interventional therapy, the cause of which is the damage of endothelial cell. The levels of ET-1,T/P and the changes of T/P value are incresed as the vascular lesion become more and more severe. The formation of postoperative thrombus is positively associated with the severity of vascular lesion.