Abstract:In 2001，a locus for congenital generalized lipodystrophy（CGL） was identified on chromosome 11q13 by positional cloning，and mutations in a novel gene were named Seipin. Human Seipin is an ER-resident protein. Expressions of seipin protein can be de－tected in adipose tissues and motor neurons. Seipin gene was originally identified as a loss-of-function gene for CGL type Ⅱ，a condi－tion characterized by severe lipodystrophy of both metabolically active and mechanical adipocytes. This paper reviewed the structure and distribution of Seipin gene as well as the features and clinical manifestations of CGL genetically defective Seipin gene. Mean－while，it disscussed the role of Seipin gene in the pathogenesis of CGL typeⅡ from the aspects of morphology and differentiation of lipid droplets.

Abstract:Objective：To obtain the mimic peptide highly binding to cholerae O1 serotype Ogawa by using purified IXiao3G6 McAb to biospan in phage display random 7 peptides library and to establish the basement for further research of the mimic peptide vaccine of vibrio cholerae. Methods：Purified IXiao3G6 McAb was used to biospan in phage display random 7 peptides library and mimic epitopes were acquired after 3 rounds of biospanning. ‘Double-antibodysandwich’ ELISA and specific inhibition test were used to analyze the combining ability and specificity of the obtained phage short peptide and the IXiao3G6 McAb. DNA sequence analysis was used to an－alyze the sequence of the positive clones and the related bioinformatic tools were used to analyze the alignments. Results：After three rounds of biospanning，the positive phages were enriched effectively. Twenty-two phage clones were positive of the 25 phage clones se－lected randomly by ‘double-antibodysandwich’ ELISA. DNA sequence analysis showed that the amino acid sequences of 20 positive clones were identical completely，which was identified as APAIPAS. Alignment analysis showed no homologous known sequence. Spe－cific inhibition test showed that the lipopolysaccharide（LPS） of cholerae O1 serotype Ogawa could inhibit the binding of the positive clones with IXiao3G6 McAb and the inhibitory intensity was enhanced by increment of the content of LPS，while the LPS of control bacteria did not show the corresponding inhibitory effect. These results further demonstrated phage display peptide could be mimetic to the antigen epitope of the LPS of cholerae O1 serotype Ogawa. Conclusions：Mimic peptide mimesis to the antigen epitope of the LPS of cholerae O1 serotype Ogawa serves as the basement for development of the mimic peptide vaccine of vibrio cholerae.

Abstract:Objective：To study influences of LIM mineralization protein（ＬＭＰ－１） on the osteogenic potential of cultured bone marrow mesenchymal stem cells（BMSCs） by constructing eukaryotic expression plasmid carrying LMP-1 and having it expressed in rabbit BMSCs. Methods：Reverse transcription- polymerase chain reation（RT-PCR） technique was used to clone hLMP-1 and hBMP-2 from human placenta tissue and plasmid with hBMP-2. Digestion and ligations with eukaryotic expression plasmid pIRES2-EGFP，pIRES2-EGFP-LMP-1 and pIRES2-EGFP-BMP-2 recombinants were constructed. BMSCs were isolated and culture-expanded from rabbit bone marrow. The third culture was chosen for study. With the help of lipofectamine，plasmids pIRES2-EGFP-LMP-1，pIRES2-EGFP-BMP-2 and pIRES2-EGFP were transfected into BMSCs. Transfective rate was estimated under fluorescence microscope and cells were tested seven days later. Expressions of hLMP-1，hBMP-2 and collagen type Ⅰ were tested with RT-PCR. Alkaline phosphatase（ALP） testing kit was used to measure ALP activity and immunohistochemical staining was used to test the expression of osteocalcin. Results：Restriction and sequencing analyses confirmed that eukaryotic expression plasmid pIRES2-EGFP-LMP-1 and pIRES2-EGFP-BMP-2 was successfully constructed. 15% transfection rate was estimated by fluorescence microscope. Groups transfected with pIRES2-EGFP-LMP-1 and pIRES2-EGFP-BMP-2 expressed hLMP-1 and hBMP-2. Both groups enhanced the activity of ALP and induced the expression of collagen type Ⅰ and osteocalcin. Conclusion：LMP-1 can promote the osteogenic potential of cultured BM－SCs，which lays a foundation for gene therapy with LMP-1.

Abstract:Objective：To construct human aquaglyceroporin 9（AQP9） recombinant plasmid and to detect its expressions in COS-7 cells in order to elucidate the role of AQP9 in pathogenesis of nonalcoholic fatty liver disease（NAFLD）. Methods：AQP9 gene was ob－tained by RT-PCR from human hepatic tissue and was cloned into pEGFP-N1 plasmid. pEGFP-N1-AQP9 recombinant plasmid was constructed，identificated and transfected into COS-7 cells. Transfection was detected by fluorescence microscopy；expressions of AQP9 were detected by RT-PCR and Western blot. Results：PCR and enzyme digestion analysis showed that AQP9 size was right. E－quencing analysis found a nonsense mutation，45 th：A→G. RT-PCR and Western blot demonstrated that AQP9 was expressed in COS-7 cells，suggesting that pEGFP-N1-AQP9 was expressed in COS-7 cells and had immunogenicity. Conclusions：pEGFP-N1-AQP9 recombinant plasmid is constructed and expressed successfully in COS-7 cells，which would lay a foundation for further study of AQP9 in the pathogenesis of NAFLD.

Abstract:Objective：To prepare a high molecular polymer microbubble MBQDs/PLGA/Her marked by quantum dots（QDs） and modified by single antibody Herceptin and to study its application in targeted experiment of human breast cancer cells MCF-7. Methods：MBQDs/PLGA/Her targeted microbubble was produced by the double emulsion method（W1/O/W2） and covalent coupling technique（EDC/NHS）. Its physi－cal properties（loading mark of QDs，cytotoxicity，connection of MBQDs/PLGA /Her to antibody，fluorescent marking performance of QDs） were determined by methods of TEM，MTT，Fourier transform infrared spectrum（FT-IR） and fluorescence microscope respectively. Results：MBQDs/PLGA/Her average diameter was （584.7±20.2） nm. TEM imaging showed that QDs was loaded in recombinant human tissue-type plasminogen activator poly lactic-glycolic acid（PLGA） particles and FT-IR spectrogram confirmed covalent coupling between Her－ceptin’s amino and PLGA’s carboxyl. Cell toxicity of MBQDs/PLGA/Her was less than that of QDs reagents（P<0.05）. Red microbubble MBQDs/PLGA/Her marked by QDs was connected round human breast cancer cells MCF-7. Conclusions：MBQDs/PLGA/Her microbubble is prepared successfully. Targeted microbubble cell toxicity is improved and QDs fluorescent marking performance is kept by loading QDs into polymer microbubble.

Abstract:Objective：To investigate efficacy of bone marrow mesenchymal stem cells（MSCs） modified by plasmid pIRESneo-EGFP-BDNF in treatment of rats with Parkinson’s disease（PD）. Methods：pIRESneo-EGFP-BDNF plasmid was established by reconstructing pEGFP（N1）-BDNF plasmid and bone marrow MSCs was transfected by electroproation. PD rat models were set up by 6-OHDA and then divided into four groups randonly：sham group，PD group，MSCs group and brain-derived neurotrophic factor（BDNF） group. Rotating behaviors of rats were induced by intraperitoneal injection of Apomorphine at 2，4，8 weeks after bone marrow MSCs transplantation or MSCs modification of plasmid pIRESneo-EGFP-BDNF through cerebral lateral ventricle. BDNF protein and tyrosine hydroxylase（TH） protein in mesencephalic nigra were measured by Western blot. Results：Plasmids pIRESneo-EGFP-BDNF were constructed successfully，which was identified by double digestion. Rotation numbers were significantly decreased in MSCs group and BDNF group than in PD group at 2，4，8 weeks after the transplantation（P<0.05），with more evident improvement in BDNF group than in MSCs group（P<0.05）. Levels of BDNF protein and TH protein in nigra at 8 weeks after PD model being intervened by cell transplantation were increased significantly in BDNF group than in MSCs group and PD group. Conclusions：Transplantation of pIRESneo-EGFP-BDNF modified MSCs through cerebral lateral ventricle can improve PD rats’ behavior and increase BDNF and TH protein levels.

Abstract:Objective：To investigate expressions of KAI1/CD82 in colorectal carcinoma and their correlation with the metastasis of col－orectal carcinoma. Methods：Tissue microarray and immunohistochemistry were used to detect expressions of KAI1/CD82 in 233 cases of colorectal carcinoma. Results：Positive rate of KAI1/CD82 expression in colorectal carcinoma was 51.5%. Expressions of KAI1/CD82 were not correlated with patients’ sex，age，depth of infiltration and lymphnode metastasis，but were correlated with differentia－tion degrees and Dukes stages（P<0.05）. Forward stepwise binary Logistic regression revealed that differentiation was the most signifi－cant influencing factor of KAI1/CD82 expression，Exp（b）=0.431，P=0.038，followed by Dukes stage，Exp（b）=0.363，P=0.043. Expres－sions of KAI1/CD82 were decreased in colorectal carcinoma with poor differentiation and at Dukes D stage. Conclusions：Expressions of KAI1/CD82 are closely correlated with differentiation degrees and stages，which are expected to become a new target for tumor prognosis.

Abstract:Objective：To study the protective effects of acetyl-L-carnitine（ALC） on ischemic brain injury in rats and oxygen-glucose deprivation（OGD） induced cells as well as the possible mechanisms. Methods：Using the table of random number，SD rats were di－vided into three groups：sham group，model group and ALC group. ALC and an equal volume of phosphate buffer were injected intraperitoneally for ALC，model and sham groups respectively at 24 h before cerebral ischemia rat model was made. TTC（2，3，5-Triphenyltetrazolium chloride） staining was used to assess the effects. In vitro，PC12 cells were divided into normal group，model group and ALC group. ALC was given at 24 h before OGD model being made. Cell viability，apoptosis and necrosis were analyzed using MTT assay，TUNEL，SYTOX staining respectively. Activities of adenosine triphosphatase（ATPase） and superoxide dismutase（SOD） as well as maleic dialdehyde（MDA） content were also detected. Results：After the pre-treatment of ALC，the infarction size was obviousely reduced（P<0.05）；in vitro，the cell viability，activities of SOD and ATPaes were increased，while the content of MDA，percentage of apoptotic and necrotic cells were decreased compared with those in OGD group（P<0.05）. Conclusions：These results suggested that ALC has protective effects on ischemic injury rats and OGD induced PC12 cells. The effects may be associated with the improvment of cell antioxidant capacity and energy metabolism as well as the supression of necrosis and apoptosis.

Abstract:Objective：To compare the quantities and functions of CD4+CD25+ regulatory T（Treg）cells between food allergy mice model and normal mice model. Methods：SPF Balb/c female mice of 4-6 weeks on egg-free diet were randomly divided into ovalbumin（OVA） group（n=8） and normal control group（n=8）. Quantitative changes of CD4+CD25+ Treg cells in the spleen mononuclear cell suspension were analyzed by flow cytometry. Levels of serum interleukin-10（IL-10） and transforming growth factor-β1（TGF-β1） in OVA mice and normal control mice were detected by enzyme-linked immunosorbent assay（ELISA）. Results：Percentage of CD4+CD25+ Treg cells in OVA group was lower than that in normal control group（P<0.05），and percentage of forkhead transcription factor3+（Foxp3+）CD4+CD25+ Treg cells in OVA group was significantly lower in OVA group than in normal control group（P＜0.01）. Levels of IL-10 and TGF-β1 were lower in OVA group than in normal control group（P＜0.05）（P＜0.01）. Conclusions：There are differences in quantities and functions of Treg cells between food allergy and normal mice.

Abstract:Objective：To observe the inhibitory effect of LKB1 gene on Hela cell proliferation and to investigate the underlying mech－anism. Methods：Hela cell lines lacking LKB1 expression were cultured in vitro and were transfected with liposome mediated LKB1 plasmid. Stable Hela cell lines constitutively expressing LKB1 protein were established by using G418 antibiotic selection pressure. Cell cycle profile and phase distribution were analyzed by flow cytometry. Protein levels of phosphorylated Rb，proliferating cell nu－clear antigen（PCNA），adenosine monophosphate activated protein kinase α（AMPK α） and phosphorylated AMPKα were analyzed by Ｗestern blot. AMPK inhibitor Compound C was also used to examine the effect of AMPK inhibition on cell proliferation. Results：Hela cell lines expressing LKB1 protein were successfully established. Constitutive expression of LKB1 in Hela stable cells led to a delayed G1/S transition，accompanied by a decrease in Rb phosphorylation. Reduced expressions of PCNA and elevated phosphoryla－tion of AMPKα were also observed in Hela stable cells. Besides，the expression of PCNA was greatly increased in Hela stable cells treated with AMPK inhibitor Compound C. Conclusion：Expression of LKB1 protein in Hela cells inhibited cell proliferation，which is mediated by LKB1 induced AMPK activation.

Abstract:Objective：To explore the role of negative regulators of toll-like receptor（TLRs） signal pathways in immunological patho-genesis of severe hepatitis B virus infection（CHB）. Methods：mRNA expressions of myeloid-differentiation-88-short（MyD88s），interleukin-1R-associated-kinase-M（IRAK-M），single-immunoglobulin-interleukin-1R-related-molecule（SIGIRR），zinc finger protein A20 and TLR4 in peripheral blood mononuclear cells（PBMCs） in 20 cases of chronic CHB，17 cases of acute-on-chronic liver failure at early stage（ACLF-E），9 cases of acute-on-chronic liver failure at late stage（ACLF-L） and 18 healthy controls were detected by real time fluorescence quantitative PCR. Serum levels of tumor necrosis factor-α（TNF-α） and interleukin-10（IL-10） were detected by ELISA assay. Results：Compared with those in healthy controls，mRNA expression levels of MyD88s，IRAK-M and A-20 as well as serum levels of TNF-α and IL-10 were unregulated with the progression of diseases in CHB patients，ACLF-E patients and ACLF-L patients（P<0.05）（P<0.01）. There was no significant difference in serum levels of IL-10 between CHB and ACLF-E（P >0.05）. mRNA expressions of TLR4 were higher in CHB patients，ACLF-E patients and ACLF-L patients than in healthy controls. mRNA expressions of TLR4 were lower in ACLF-L patients than in ACLF-E patients（P<0.05）. Conclusions：Negative regulators of TLRs signaling pathway participate in the pathogenesis of chronic hepatitis and liver failure and upregulation of negative regulators may lead to immunosuppressive in patients with liver failure.

Abstract:Objective：To investigate the expressions and significances of cyclin kinase subunit1（CKS1） and CyclinD1 in breast car－cinoma. Methods：Expressions of CKS1 and CyclinD1 in 105 cases of breast carcinoma were assessed by immunohistochemical technique（SP method） and those in 100 cases of breast fibroadenoma（control group） were simultaneously detected. Expressions and significances of CKS1 and CyclinD1 in breast carcinoma tissues were analyzed. Results：Positive rates of CKS1 and CyclinD1 in breast carcinoma were 80.9%（85/105） and 85.7%（90/105）. Positive rates of CKS1 and CyclinD1 in breast fibroadenoma were 22%（22/100） and 19%（19/100）. Positive expressions of two genes were in correlation with lymphatic vessel invasion and lymph node metas－tasis in breast carcinoma. Positive rates of CKS1 and CyclinD1 in breast carcinoma were higher than those in breast fibroadenoma（P<0.05）. Positive expressions of CKS1 and CyclinD1 in breast carcinoma were in positive correlation（r=0.677，P<0.05）. Conclusions：Positive expressions of CKS1 and CyclinD1 are significantly higher in breast carcinoma than in breast fibroadenoma. Positive expres－sions of CKS1 and CyclinD1 in breast carcinoma are positively related with lymphatic vessel invasion and metastasis of lymph node. Expressions of CKS1 and CyclinD1 may play a role in evaluating the prognosis and guiding the clinical treatment for breast carcinoma.

Abstract:Objective：To observe the therapeutic effects of Radix Astragali injection combined with Acitretin on psoriasis mice model. Methods：Effects of Radix Astragali injection combined with Acitretin on proliferation of vaginal epithelium，proliferating cell nu－clear antigen（PCNA） expression，differentiation of tail scale epidermis and plasma endothelin-1（ET-1） were assessed in mice model. Results：Radix Astragali injection combined with Acitretin had effects on all 4 indices mentioned above. Radix Astragali injection com－bined with Acitretin can significant inhibit vaginal epithelium proliferation，PCNA expression and plasma ET-1 as well as promote the differentiation of tail scale epidermis with statistical differences compared with those in control group（P<0.001）. Conclusions：Radix Astragali injection combined with Acitretin can improve and reverse psoriatic pathology model in mice，the mechanism of which may relate with the inhibition of epidermal cell over-proliferation and the promotion of granular layer formation. Its therapeutic effects may be explained by bloclking multiple pathogenetic linkages.

Abstract:Objective：To explore the feasibility of high-intensity focused ultrasound（HIFＵ） treatment for breast fibroma accompanied with hyperplasia of mammary glands. Methods：Nine patients diagnosed as breast fibroma accompanied with hyperplasia of mammary glands accepted HIFU treatment and then breast ultrasonography or mammography to follow up changes of pain and nodules’ size in the breast. Results：Pain in nine patients was relieved to varying degrees within three months. Curative effect was sustained for 12 to 24 months（average of 16 months）. Conclusions：It is safe and effective to ablate breast fibroma with HIFU，which is a new approach to treating hyperplasia of mammary glands and relieving breast pain obviously.

Abstract:Objective：To evaluate the safety and reliability of applying Perclose ProGlide suture homeostasis device（Abbott，USA） after percutaneous femoral artery puncture and endovascular graft exclusion（EVGE） for aortic dissection. Methods：Totally 200 patients with Stanford type B aortic dissection from 2004 to 2012 in our hospital were enrolled and were randomized into control group（n=100） and observation group（n=100） according to EVGE for aortic dissection under local anesthesia or general anesthesia. Skin in in－guinal region of patients in control group was longitudinally incised 4.0-6.0 cm and was exposed 2.0-3.0 cm；femoral artery puncture was performed soon to establish femoral artery path. Patients in observation group underwent femoral artery puncture to establish femoral artery path and two sets of Perclose ProGlide devices were conventionally used after the operation. Results：Success rate of two sets of Perclose ProGlide suture devices in observation group was 95.0%（95/100）. Operation time in control group and observation group was （113.0±16.0） min and （90.0±12.1） min respectively，with significant statistical differences（P<0.01）. Femoral artery path establishment time was （14.0±1.3） min and （4.3±0.8） min respectively，with significant statistical differences（P<0.01）. Vascu－lar processing time after exiting the sheath was （8.9±1.3） min and （3.0±0.7） min respectively，with significant statistical differ－ences（P<0.01）. Postoperative braking time was （18.5±1.9） h and （7.7±2.3） h respectively，with significant statistical differences（P<0.01）. Postoperative average hospitalization time was （5.2±1.0） d and （3.3 ± 0.8） d respectively，with significant statisti－cal differences（P<0.01）. Complications incidences were 5% and 4% respectively，without statistically differences（P >0.05）. Wound discomfort was respectively observed in 36 cases（36.0%） and 17 cases（17.0%），with significant statistically differences（P<0.01）. Conclusions：Clinical application of the Perclose ProGlide suture hemostasis device after EVGE for aortic dissection is a safe and reliable method，which can reduce operation time，femoral artery path establishment time，vascular processing time after exiting the sheath，brake time，postoperative hospitalization time with low complication incidences.

Abstract:Objective：To investigate the relationship between coronary heart disease（CHD） risk and serum angiotensinⅡ（AngⅡ） con－centration in male patients with nonalcoholic fatty liver disease（NAFLD）. Methods：All participants were non-smoking males，includ－ing 35 normal controls and 85 NAFLD patients. Fasting serum Ang Ⅱ concentration was measured，insulin resistance（IR） was esti－mated by homeostasis model assessment of insulin resistance（HOMA-IR） and 10-year CHD risk was assessed by Framingham risk score（FRS）. According to the international diabetes federation criteria for metabolic syndrome（MS），NAFLD patients were subdivided into MS（+） and MS（-） groups. According to FRS，NAFLD patients were divided into FRS<0 and FRS≥0 groups. Results：Serum AngⅡ concentration was significantly higher in MS（-） and FRS≥0 groups than in control group（P<0.001） and FRS<0 group（P=0.017），respectively. There was no significant difference in Ang Ⅱ concentration between MS（+） and MS（-） groups. FRS was sig－nificantly higher in MS（-） than in control group（P=0.035），significantly higher in MS（+） group than in MS（-） group（P=0.042）. Ten-year CHD incidence risk ratio was positively associated with fasting plasma insulin，HOMA-IR and serum AngⅡ concentration （r’=0.248，0.335，0.222，P’=0.009，<0.001，0.02）. Conclusions：Increased 10-year CHD risk in male NAFLD patients is positively associated with IR and serum AngⅡ concentration. Inhibition of AngⅡ may decrease CHD risk in male NAFLD patients.

Abstract:Objective：To investigate the efficacy of different incising and dressing styles of minimally invasive surgery for axillary osmidrosis and their effects on complications. Methods：Totally 124 patients with axillary osmidmsis（245 sides） undergoing different minimally invasive surgeries were analyzed and were randomly divided into three groups. Three incising styles of and three dressing styles were performed on patients in the three groups respectively and clinical efficacy as well as short and long term complication incidences were compared. Incising styles included bilateral ‘W’ shape incision，unilaterally ‘W’ shape incision on the inferior border and vertical incision through the axilla center. The dressing styles included ‘six points’ suturing dressing，multi-poles rubber drainage dressing and ‘rivet-type’ fixing and compressing dressing. Results：Axillary sweating and hair growth were reduced in all patients. No statistical significance in clinical efficacy was observed among different styles of incising and dressing（P ＞0.05）. Postoperative com－plication incidences were 17.14% in short term and 14.29% in long term. Postoperative complication incidences in short and long term were lower in bilateral ‘W’ shape incision than in the other two incising styles（P<0.05）. Postoperative complication incidence in short term was lower in multi-poles rubber drainage dressing than in the other two dressing styles（P<0.05），but no difference was observed in long term among the three styles（P >0.05）. Interactive effects of different incising and dressing styles on short and long term complications were not verified. Conclusions：There was no significant difference in clinical efficacy among three incising and dressing styles. Bilateral ‘W’ shape incision can reduce the short and long term complications and multi-poles rubber drainage dress－ing can reduce short term complication but has no effect on long term complication.

Abstract:Objective：To establish a joint non-invasive clinical method and system to assess and evaluate the severity of hepatic fibro－sis of patiants with non-alcoholic fatty liver disease（NAFLD）. Methods：Totally 104 patients with non-alcoholic fatty hepatic fibrosis were divided according to different histopathological stages.Hepatic fibrosis relation indexes，tissue inhibitors of matrix metallopro－teinase-1（TIMP-1），hepatic B ultrasond and liver diffusion weighted imaging（DWI）were detected. Results：There were consider－able correlations between hepatic ultrasond and liver fibrosis grading，with a correlation coefficient of 0.56. Joint non-invasive method combing DWI，liver fibrosis markers and TIMP-1 corrected 84.69% of all trials. Conclusions：This joint non-invasive assessment method is practical and repeatable and is recommended to be a clinical method for diagnosing liver fibrosis.

Abstract:Objective：To discuss the short-term efficacy of loop electrosurgical excision procedure（LEEP） in the treatment of human papillomavirus（HPV）16，18 persistent infection after the operation for high-grade cervical intraepithelial neoplasia（CIN） and to ex－plore the influcing factor of HPV16，18 persistent infection. Methods：Totally 167 cases of CINⅡ/Ⅲ were confirmed by coloposcopy cervical biopsy and received treatment in the department of gynecology of Liuzhou People’s Hospital from January 2007 to June 2009 were enrolled. HPV16，18 DNA was detected in all patients and cervical LEEP was performed on all of them. Thinprep cytologic test（TCT），HPV16，18 DNA detection and follow-up were applied at six months after the operation. Results：HPV infection rate was 77.95l% preoperatively and 7.87% at six months after the operation in the follow-up visit. Cervix biopsy was performed in 43 pa－tients with persistent positive HPV-DNA，abnormal TCT or positive surgical margin. Rate of residual lesions was 9.3%. Conclusions：①HPV16，18 infection in female genital tract can be effectively cleared by LEEP. ②Influencing factors of persistent HPV16，18 in－fection after the treatment of high-grade CIN by LEEP include age，age at first sexual activity，multiple delivery and positive margin involvement. ③Positive margin involvement is the risk factor for the residual lesion of high-grade CIN after treating by LEEP.

Abstract:Objective：To observe the effects of rhBMP-2-coated Kirschner wire on the recovery of long tubal bone fracture. Methods：Totally 36 new southwest big white rabbits were randomly divided into two groups. Kirschner wire coated with rhBMP-2/poly-D，L-lactide（PDLLA） was inserted into the medullary cavity of fractured tibia in experimental group while Kirschner wire coated with only PDLLA was inserted into the medullary cavity in control group. Bone morphology and bending strength were analyzed and measured at 4，8，12 weeks postoperatively. Results：Osteogenesis was better in experimental group compared with that in control group；fracture stress and maximum stress were higher in experimental group compared with those in control group（P<0.05）. Conclusions：Kirschn－er wire coated with rhBMP-2 and PDLLA can promote the bone fracture recovery and shorten the fracture recovery time with the dis－solution of PDLLA and release of rhBMP-2.

Abstract:Objective：To observe the efficacy and value of applying autologous free skin graft in the treatment of refractory wound. Methods：Totally 352 patients with refractory wound were selected. Wound treatment，basic disease treatment and systemic support were performed until the granulation became fresh. Autologous free skin graft（the surface layer and middle thick layer） was provided according to parts of the wound. Pressure dress was followed soon by using burn dressing. Results：Among the 352 cases，322 under－went stageⅠdebridement and skin graft，30 underwent stageⅠdebridement and stageⅡskin graft. Among 340 cases of skin graft，90% flaps were survived postoperatively. Twelve cases had less than 1/3-1/2 flaps died，however，the wound was healed and the patients were discharged by dressing or replanting. Average hospital duration was （16 ± 2） d，and average postoperative recovery time was （19 ± 3） d. Conclusion：Autologous free skin graft can promote the recovery of refractory wound and improve the survival rate.

Abstract:Objective：To investigate the effects of estrogen receptor（ER） and progesterone receptor（PR） on long term prognosis of young patients with breast cancinoma. Methods：ER and PR statuses in 370 patients with breast cancinoma（younger than 35 year） were immunohistochemical examined and followed up. Results：Totally 366 out of 370 patients were followed up for 24 to 288 months，with an average of 132.64 months. ER and PR positive rates were 44.86%（166/370）and 38.11%（141/370）respectively. ER and PR statuses were not associated with 2-year survival rate and 5-year survival rate. There were differences in 8-，10-，15-year survival rate between ER（+） and ER（-）（ χ2=5.992，P=0.014； χ2=7.025，P=0.008； χ2=6.221，P=0.013）. Eight-year survival rate in PR（+） was higher than that in PR（-）（ χ2=9.552，P=0.002）；2-year survival rate in ER（+）PR（+） was not different from that in ER（-）PR（-）（ χ2=1.963，P=0.161）；but 5-year and 8-year survival rates in ER（+）PR（+） were higher than those in ER（-）PR（-）（ χ2=7.365，P=0.007； χ2=5.236，P=0.022）. Conclusions：ER and PR are important long term prognostic factors in young patients with breast canci－noma. Simultaneous exam of ER and PR is of higher prognostic value. ER（-）PR（-） and ER（-）PR（-） indicate poor prognosis，therefore auxiliary treatment including chemotherapy should actively be put in force and followe up should be strengthen in order to improve the prognosis.

Abstract:Objective：To observe and compare the state of marginal microleakage between fluoride-containing resin sealant and glass ionomer sealant in vitro. Methods：Totally 30 newly extracted noncarious human the third molars were randomly divided into three groups with 10 samples in each group. The occlusional surfaces of the specimens were sealed with ClinproTM Sealant，Fuji Ⅸ GP and Concise，respectively. After 125 cycles of thermo-cycling，all specimens were immersed in 1% solution of methylene blue dye for 48 h then the microleakage of each specimen was measured under stereomicroscope. Results：Results of dye penetration revealed that there was no significant difference in microleakage among two test groups and control group（P >0.05） and there was no significant difference between two test groups（P >0.05），either. Conclusions：All three types of pit and fissure sealants showed lower microleakage. The tooth surface fitness of glass ionomer cement（Fuji Ⅸ GP） is comparatively similar to resin sealants，with satisfactory sealing ability. It’s worth promoting glass ionomer material as pit and fissure sealant in clinics.

Abstract:Objective：To investigate clinical features of pulmonary complications following liver transplantation in order to guide clini－cal intervention and to increase patients’ survival rate. Methods：Clinical data of 68 liver transplant recipients in our hospital from November 2006 to November 2011 were studied retrospectively. Clinical features of postoperative pulmonary complications including incidences，time of onset，risk factors or causes of morbidity，treatment and outcome were studied and summarized. Results：Sixty（88.2%）patients developed at least one pulmonary complication following liver transplantation. The most frequent complication was pleural ef－fusion（59，86.8%），followed by pneumonia（15，22.1%），pulmonary edema（13，19.1%），respiratory tract bleeding（2，2.9%）and at－electasis（1，1.5%）. Conclusions：Pleural effusion，pneumonia and pulmonary edema are common pulmonary complications following liver transplantation. Patients who develop pneumonia have poor prognosis.

Abstract:Objectives：To explore psychological and behavior patterns of children with malignant tumor at various ages as well as their influencing factors in order to provide references for clinic treatment. Methods：Totally 120 children with malignant tumor were in－vestigated with Rutter behavior questionnaire. Together with clinic examination and intensive talks，the major influencing factors were clarified. Results：Among the 120 children，detection rate of behavior problem was significantly higher in 54.6% aged under three，61.5% aged between four to seven，24.3 aged between eight to fourteen than in control group（10.7%），with statistical significances（P＜0.01）. Conclusions：Major influencing factors for behavior problems of children with malignant tumor include education methods of parents，family intimacy，responses of both children and parents to tumors，selection of care-taker. Besides，individual psychological and physical treatment should be provided to enhance their responding ability and cultivate their characteristics，ultimately promote their sound development.