Abstract:Objective：To study the histopathological and ultrastructural changes in the hippocampus of rats with vascular dementia（VD） by light and electron microscopy. Methods：Totally 60 SD rats were randomly divided into sham-operated group（n=10） and model group（n=50）. VD models were constructed by permanent bilateral common carotid artery ligation and 10 rats were killed at each time point（1，7，14，21，28 d） after operation（1，7，14，21，28 d model groups）. Rats in sham-operated group were only separat－ed，not ligated bilateral common carotid arteries. Histopathological and ultrastructural changes in the hippocampus were observed with microscopy and electron microscopy respectively at each time point with 10 killed VD rats. Results：Numbers of neurons in CA1 re－gion of rats’ hippocampus in model group were decreased significantly compared with those in sham-operated group，being the fewest in 14 d model group（P=0.000）. Ultrastructural changes demonstrated the pyknosis and degeneration of neurons，however，with the re－covery of cerebral blood flow supply，numbers of neurons were gradually increased and less neuron degeneration was observed with electron microscopy. Conclusions：At early stage of chronic cerebral hypoperfusion，degereration of neurons in the hippocampus is re－versible，which provides theoretic basis for early clinical treatment of chronic cerebral hypoperfusion in prevention of VD.

Abstract:Objective：To explore if genetic variation in chromosome 8q24（rs987525） and MAFB（rs13041247） gene contributes to nonsyndromic cleft lip with or without cleft palate（NSCL/P）. Methods：Study group consisted of 369 NSCL/P patients，their parents （303 fathers，311 mothers and 158 complete core families） and 433 controls. These 2 single nucleotide polymorphisms（SNPs） were genotyped by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. Genotypic and allelic distributions and comparisons of rs987525 and rs13041247 in patients，their parents and controls were analyzed by X2 test and transmission dise－quilibrium test（TDT） and their association with NSCL/P was evaluated. Results：Case-control analysis found that there were signifi－cant differences in the frequency distributions of both genotypes and alleles in cleft lip，cleft lip with or without cleft palate groups of rs13041247 in MAFB gene（P=0.02，P=0.04，P=0.01，P=0.04），however，there was no statistical difference in the frequency distribu－tions of both genotypes and alleles in cleft lip group（P=0.25，P=0.51）. There was neither allelic nor genotypic association between rs987525 in chromosome 8q24 and NSCL/P（P=0.07，P=0.20，P=0.58，P=0.33，P=0.35，P=0.59）. TDT tests demonstrated that rs13041247 C allele in the locus of cleft lip patients had transmission（P=0.03）. rs987525 A allele in cleft lip，cleft palate and cleft lip with or without cleft palate patients had transmission（P=0.00，P=0.04，P=0.00）. Conclusions：MAFB gene rs13041247 SNP is associated with NSCL/P in Ningxia population.

Abstract:Objective：To culture in vitro and identify rat bone marrow mesenchymal stem cells（BMSCs），to study their biological char－acteristics and to tentatively explore the feasibility of differentiation of BMSCs into neural stem cells（NSCs） induced by acidic fibrob－last growth factor（aFGF）. Methods：Rat BMSCs were isolated and cultured by the whole bone marrow adherent culture method. Mor－phological characteristics of BMSCs were observed under the inverted phase contrast microscope and cell growth curves were drawn by MTT method. Cell cycle and surface markers were detected with flow cytometry（FCM）. Rat BMSCs were induced to differentiate into NSCs by 80 ng/ml aFGF of DMEM/F12 medium. Cell morphological changes were continuously observed after using aFGF. Im－munofluorescence staining was used to detect the expressions of Nestin and neuron specific enolase（NSE）. Results：Rat BMSCs showed adherent growth and was spindle shaped or in polygonal appearance. Growth curves of the 1st，3rd，5th-generation BMSCs were in S shape and their activities were not significantly different. FCM showed that 94.34% BMSCs were in G0/G1 phase of the cell cycle，having strong proliferation ability. FCM detected that CD29，CD54 and CD90 were positively expressed，but CD45 was negative－ly expressed. At 6 h after the induction，cell bodies became to shrink into oval or sphere，and stretched out long and thin protrusions. Immunofluorescence staining showed that Nestin was positively expressed. Bipolar and multipolar cells were constantly increased after continuous induction. At 6 d after the induction，cells interconnected into a mesh and became typical neuron-like cells. NSE was posi－tively expressed. Conclusions：Whole bone marrow adherent culture method can cultivate highly purified rat BMSCs with stem cells’ characteristics：growing stably，proliferating rapidly and be multiple-passaged. By the induction of aFGF，BMSCs have the potentiality of differentiating into NSCs，which can further differentiate into neuron-like cells.

Abstract:Objective：To investigate the effects of different concentrations of sevoflurane anesthesia on cognitive function in aged rats. Methods：Eighty SD rats were randomy divided into 5 groups：0% sevoflurane group（C，n=16），1% sevoflurane group（S1，n=16），2% sevoflurane group（S2，n=16），3% sevoflurane group（S3，n=16） and 4% sevoflurane group（S4，n=16）. All rats received anesthesia for 6 h. Cognitive function was tested with Morris watermaze. Amyloid precursor protein（APP），β-amyloid peptide（Aβ） and human beta-site APP-cleaving enzyme-1（BACE-1） expressions in the hippocampus were determined with ELISA. Results：（1）Morris watermaze test and spatial probe test demonstrated that both latency and the number of seeking for hidden-platform were significantly decreased in S3 and S4 groups than in control group（P<0.05），but no significant difference between S1 and S2 groups was found（P >0.05）. （2）Ex－pressions of APP and Aβ in the hippocampus were increased with increase of concentrations（P<0.05）. （3）Expressions of BACE-1 in hippocampal were significant increased in S3 and S4 groups than in control group（P<0.05）. Conclusions：Sevoflurane inhibits cognitive function of aged rats in dose-dependent manner. These results suggest high concentration sevoflurane could induce APP and Aβ and result in the up-regulation of BACE-1 expressions.

Abstract:Objective：To investigate the inhibition effects of methylase inhibitor 5-aza-2-deoxycytidine on human keloid fibroblasts，and the effects of 5-aza-2-deoxycytidine on related cytokines of human keloid fibroblasts. Methods：Human keloid and surrounding normal skin samples were collected for primary cultured fibroblasts. Cells were divided into keloid fibroblast experimental group，normal skin fibroblast experimental group，keloid fibroblast control group and normal skin fibroblast control group. 5-aza-2-deoxycytidine was used to intervene the experimental groups. Expressions of TGF-β1mRNA and Smad7mRNA in each group were detected with RT-PCR. Effects of 5-Aza-2-deoxycytidine on the cell cycle and apoptosis of fibroblasts were analyzed with flow cytometry（FCM）. Results：Compared with those of normal skin fibroblast，expressions of TGF-β1mRNA was increased（P=0.001） but Smad7mRNA were decreased（P=0.001） in keloid fibroblast，meanwhile the proportion of cells in G0/G1 stage（P=0.035） and apoptosis cells were decreased（P=0.006）. Expressions of TGF-β1mRNA were decreased（P=0.003） and those of Smad7mRNA were elevated（P=0.000） in keloid fibroblasts intervened by 5-aza-2-deoxycytidine. FCM demonstrated that the proportions of cells in G0/G1 stage and apoptosis cells were increased（P=0.000，P=0.047） in keloid fibroblasts intervened by 5-aza-2-deoxycytidine. But changes in normal skin fi－broblast group were insignificant（P >0.05）. Conclusions：Methylaze inhibitors，5-aza-2-deoxycytidine may inhibit the proliferation and promote the apoptosis of keloid fibroblast as well as influence expressions of its related cytokines，however，the effects on normal skin fibroblasts are insignificant. Pathogenesis of keloid may relate with the methylation of certain genes. 5-aza-2-deoxycytidine may be a new choice in the treatment of pathological scar.

Abstract:Objective：To investigate levels of asymmetric dimethylarginines（ADMA） in serum of women with and without polycystic ovary syndrome（PCOS），PCOS model rats and control rats，to explore expressions of protein aiginine methyltransferase 4（PRMT4） in ovarian tissues of PCOS model rats and to explore relationship between levels of ADMA and PRMT4 and PCOS. Methods：ELISA was used to investigate levels of ADMA in serum of 49 PCOS patients，20 normal patients，20 PCOS model rats and 20 normal rats. Ex－pressions of PRMT4 in ovarian tissues were detected by SP immunohistochemical method（including 20 PCOS model rats and 20 con－trol rats）. Results：（１）In human serum，ADMA concentrations were （0.49±0.35）umol/L and （0.26±0.09） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. （２）In rat serum，ADMA concentrations were （0.55±0.14） umol/L and （0.23±0.08） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. （３）In rat ovarian tissues，PRMT4 integral optical density values were （44.96±9.40） umol/L and （18.27±3.83） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. Conclusions：In ovar－ian tissues，increased expressions of PRMT4 may bring about elevated ADMA level in serum，which causes endothelial dysfunction of PCOS and affects PCOS development.

Abstract:Objective：To observe the effects of sevoflurane inhalation in advance on pulmonary function and oxidative stress responses during one-lung ventilation（OLV）. Methods：Sixty patients preparing to undergo radical esophageal cancer resection were randomly divided into 3 groups equally（n=20）：sevoflurane inhalation in advance group（group SP）；propofol group（group P）；sevoflurane group（group S）. Anesthesia was induced with midazolan 0.04 mg/kg，fentanyl 3-4 μg/kg and propofol was administered by target con－trolled infusion with target blood concertration of 3 μg/ml. Tracheal intubation was facilitated with rocuronium of 0.6-0.8 mg/kg. Anesthesia was maintained with propofol at a target blood concertration of 3 μg/ml in group P or inhalation of sevoflurane（1.0%-3.0%） in group S. Anesthesia was maintained with the inhalation of sevoflurane（1.0%-2.0%） before OLV and with propofol after OLV in group SP. Remifentanil was infused by target controlled and atracurium was given intravenously and intermittently during op－eration in each groups. Bispectral index was maintained at 40-60. Blood samples were taken from radial artery and right atrial blood at 6 time points：after the induction of anesthesia（baseline，T1），before OLV（T2），at 30 min（T3），60 min（T4） after OLV，before the end of OLV（T5） and after operation（T6） to measure blood gases and to calculate Qs/Qt. Serum malondialdehyde（MDA） concen－trations and superoxide dismutase（SOD） activity were measured at T1，T3-T5. Results：Qs/Qt and MDA concentrations were significantly higher while PaO2、SOD activity were significantly lower during operation（from T3 to T5） compared with those at T1 in three groups（P<0.01）. Qs/Qt at T4 and MDA at T5 were significantly lower while SOD was significantly higher in SP group than in group P and group S（P<0.05）. Qs/Qt was significantly lower at T3 while was significantly higher at T5 in group S than in group P. There was no differ－ence in PaO2 among the three groups. Conclusions：Sevoflurane inhalation in advance before OLV could reduce intrapulmonary shunt and oxidative stress during OLV in patients undergoing esoph-ageal cancer resection.

Abstract:Objective：To understand promoter methylation status of thyroid hormone receptor β1（TRβ1） gene in intraductal prolifera－tive lesions and invasive ductal carcinoma of breast and to discuss its relationship with breast cancer. Methods：By methylation-spe－cific polymerase chain reaction（MSP），we detected promoter methylation status of TRβ1 gene in mammary tissues of 40 cases of in－filtrating ductal carcinoma（IDC），12 cases of usual ductal hyperplasia（UDH），8 cases of atpical ductal hyperplasia（ADH），24 cases of duetal carcinoma in situ（DCIS） and 10 cases of healthy adult women. Results：Methylation rates of TRβ1 gene were 0.0%（0/10），16.7%（2/12），37.5%（3/8），62.5%（15/24） and 80.0%（32/40） in healthy breast，UDH，ADH，DCIS，and IDC respectively. There were significant differences in methylation rate of TRβ1 gene between healthy breast and DCIS，IDC（P=0.001，P=0.000）. There was no sta－tistical difference in methylation rate of TRβ1 gene between UDH and DCIS（P=0.009）. There were statistical differences in methyla－tion rate of TRβ1 gene between UDH and IDC（P=0.000）. Conclusions：Promoter methylation of TRβ1 gene is an early event in the development of breast carcinoma and may play an important biological role in breast carcinogenesis.

Abstract:Objective：To explore the effects of celecoxib，ibuprofen and indomethcin on expressions of interleukin-1β（IL-1β），tumor necrosis factor-α（TNF-α） and interleukin-1α（IL-1α） in osteoarthritic chondrocytes of Wistar rats. Methods：One hundred and thirty Wistar rats were randomly divided into 4 groups：celecoxib（CE） group，ibuprofen（IBP） group，indomethacin group（IN） and normal saline（NS） group. Osteoarthritis（OA） was induced by the excision of the left achilles tendon. In the 3rd，6th and 9th month of the treatment after surgically induced OA，the articular cartilage was observed with immunohischemical method to estimate the expres－sions of IL-1β，TNF-α and IL-1α in osteoarthritic chondrocytes of 4 groups. Results：①In the 3rd month，CE exerted no remarkable effects on IL-1β and TNF-α but suppressed expressions of IL-1α；IBP promoted expressions of IL-1β and TNF-α but exerted no re－markable effects on IL-1α；IN mildly promoted expressions of IL-1β，enhanced expressions of TNF-α but exerted no remarkable ef－fects on IL-1α. ②In the 6th month，CE exerted no remarkable effects on IL-1β and mildly promoted TNF-α and IL-1α expressions；IBP promoted expressions of IL-1β（mildly） and TNF-α but mildly suppressed expressions of IL-1α；IN suppressed expressions of IL-1β（mildly），IL-1α and TNF-α（mildly）. ③In the 9th month，CE suppressed expressions of IL-1β（significantly），TNF-α and IL-1α；IBP suppressed expressions of IL-1β，TNF-α and IL-1α（mildly）. Conclusions：During the long-term use of three kinds of nons－teroidal anti-inflammatory drugs in OA，CE can inhibit the expression of IL-1β，TNF-α and IL-1α in osteoarthritic chondrocytes and consequently mitigate the development of OA. Though，IBP and IN exert different influences on expressions of IL-1β，TNF-α and IL-1α during different periods，the overall suppression effects are not obvious.

Abstract:Objective：To investigate the protective effects of propofol derivative cqmu-039 on cerebral ischemia-reperfusion injury in SD rats and its mechanisms. Methods：A total of 40 SD rats were randomly divided into 5 groups：sham surgery group，cerebral is－chemia-reperfusion（IR） model group，low（6 mg/kg），medium（12 mg/kg） and high（24 mg/kg） doses of cqmu-039 groups. IR model was generated via the surgery of middle cerebral artery occlusion（MCAO） for 2 h and then reperfusion of blood for 24 h. Sham surgery was performed in the same way as IR model without implanting the embolus. Three different doses of cqmu-039 were injected imme－diately after the IR surgery was performed. Protective effects of cqmu-039 were evaluated through the neurological behavioral score，2，3，5-triphenyltetrazolium chloride（TTC） staining and expressions of glial cell derived neurotrophic factor（GDNF） and glial fibril－lary acidic protein（GFAP） in the hippocampus. Results：Neuronal injury was significantly improved in cqmu-039 groups than in model group. In addition，lower neurological behavioral scores，smaller cerebral infarct size and higher expressions of GDNF in CA1，CA3 and cortex regions were observed in cqmu-039 groups than in model group（P<0.05，P<0.01，P<0.01）. Expressions of GFAP in CA1，CA3 and dentate gyrus regions in cqmu-039 groups were decreasent in a dose-dependent manner. Conclusions：Propofol deriva－tive cqmu-039 can effectively improve cerebral ischemia-reperfusion injury of rats through increasing expressions of GDNF and de－creasing expressions of GFAP.

Abstract:Objective：To investigate the correlation between inducible nitric oxide synthase（iNOS） gene polymorphism and the devel－opment of coronary artery lesion（CAL） in Kawasaki disease（KD）. Methods：Totally 146 patients with KD in the Children’s Hospital in Chongqing Medical University were included as KD group and 119 healthy children at the same period as control group. There were 79 KD patients with CAL as CAL group and 67 patients without CAL as NCAL group. SNPs of iNOS gene loci -1026C/A and +2087G/A were genotyped by using MassARRAY-IPLEX technology and matrix-assisted laser desorption ionization time of flight inass spec－trometry platform（MALDI-TOF-MS）. Relation between gene polymorphisms and the development of CAL in KD were analyzed. Plas－ma iNOS level was determined by ELISA，plasma NO level was measured by nitric acid reductase and expression levels were com－pared between groups. Results：Plasma iNOS level was significantly lower in control group（48.02±31.46） U/L than that in CAL group（90.29±47.68） U/L and NCAL group（81.46±41.32） U/L（P=0.000，0.000）. Plasma NO level was not significantly different be－tween groups（F=3.003，P=0.052）. Frequencies of the A minor allele and GA+AA genotypes of +2087G/A were higher in CAL group than in NCAL group（P=0.010，0.013）；both can significantly reduce the risk of CAL compared with G allele and GG genotype[OR（95%CI）=0.41（0.21-0.82），0.42（0.19-0.92），P=0.010，0.029]. Frequencies of haplotype -1026C/+2087A were different between CAL group and NCAL group（P=0.010）. -1026C/+2087A haplotype was associated with a significantly decreased risk of CAL[OR（95%CI）=0.45（0.22-0.91），P=0.030]. Conclusions：Results suggested that plasma iNOS is important in the development of vasculitis and CAL in KD. iNOS gene +2087G/A polymorphism and its -1026C/+2087A haplotype are associated with the development of CAL in KD. +2087A allele may significantly reduce the risk of CAL by lowering plasma iNOS level and reducing NO production.

Abstract:Objective：To discuss the effects of treadmill exercises on scores of neurological function，contents of 5-hydroxytryptamine（5-HT） and expressions of 5-HT1A receptor and synaptophysin in the hippocampus of rats after cerebral infarction. Methods：A to－tal of 60 rats were randomly divided into three groups：permanent middle cerebral artery occlusion（pMCAO） group（n=20），pMCAO and treadmill exercise（pMCAO+Ex） group（n=20） and sham-operated group（n=20）. Middle cerebral artery occlusion（MCAO） method was used to establish focal cerebral infarction model. Rats in pMCAO+Ex group underwent treadmill exercise on the 3rd d after model establishment for 16 d. Neurological function was evaluated using modified neurological severity scores（mNSS）. HE stain－ing and nissel staining were used to observe neuron damages in CA2 region. High performance liquid chromatography-electrochemi－cal detection system was used to determine levels of 5-HT in ischemia hippocampus tissues. Protein levels of 5-HT1A receptor and synaptophysin were measured by Western blot and mRNA levels of 5-HT1A receptor were determined by reverse transcription- poly－merase chain reaction. Results：mNSS was lower in pMCAO+Ex group than in pMCAO group on the 19th d post MCAO（P<0.001）. Both stainings showed that the morphologies of neurons in CA2 region were normal in sham-operated group and irregular in pMCAO group with parts of neurons being obviously damaged；after treadmill exercise，the damage was alleviated. Contents of 5-HT was higher in pMCAO+Ex group（14.5±1.71） ng/ml than in pMCAO group（7.13±0.71） ng/ml（P<0.001）. Protein and mRNA expres－sions of 5-HT1A receptor and protein expressions of synaptophysin were upregulated after treadmill exercise compared with those in pMCAO group（P<0.05）. Conclusions：Treadmill exercises could improve neurological function and increase contents of 5-HT and levels of 5-HT1A receptor in the hippocampus to improve synaptic plasticity.

Abstract:Objectives：To study the effects of candesartan cilexetil（CAN） on vascular endothelial function in rats with acute myocar－dial infarction. Methods：Rats were randomized into acute myocardial infarction（AMI） group，sham-operation group and CAN group. Rats were sacrificed at two weeks after the intervention and blood pressure（BP），levels of blood nitric oxide（NO） and angiotension Ⅱ（AngⅡ），activities of nitric oxide synthase（NOS），endothelial vasomotor function of isolated thoracic aorta strips were explored. Re－sults：Compared with those in sham-operation group，levels of serum NO and activities of NOS were lower，function of endothelium-dependent diastoles（EDDs） was decreased seriously while levels of plasma AngⅡ were distinctly higher in AMI group，with significant statistical differences（P=0.000 4）. After CAN treatment，levels of serum NO and activities of NOS were obviously higher，almost reaching the similar levels in sham-operation group；meanwhile function of EDDs in isolated aortic strips was improved greatly（P=0.000 9） and levels of plasma AngⅡ were increased significantly，however，BP in all groups was not affected. Conclusions：CAN can greatly improve the disordered endothelial function of rats with acute myocardial infarction without affecting BP.

Abstract:Objective：To explore the risk factors of congenital heart disease（CHD） and to provide references for research in the etiol－ogy of CHD and its scientific precautions. Methods：A hospital-based CHD 1∶1 matched case-control study was conducted. Patients were diagnosed by echocardiogram，cardiac catheterization or surgery and controls with no congenital disease were selected at the corresponding period. Children’s parents were interviewed with a unified face-to-face questionnaire. All factors were analyzed by univariate and multivariate conditional Logistic regression；risk factors associated with CHD were screened out. Results：Information of 260 pairs of children with CHD and controls were analyzed. All factors were analyzed by univariate Logistic regression at a=0.05 level and 21 suspected risk factors were initially screened out then multivariate conditional Logistic regression was used to do multi－variate comprehensive analysis. Results indicated that maternal age（>35 years：OR=3.075，P=0.010；<20 years：OR=5.084，P=0.012），passive smoking（small amount：OR=1.728，P=0.040；large amount：OR=3.903，P=0.000） and catching a cold in early pregnancy（OR=1.787，P=0.023） were the risk factors of CHD；taking folic acid regularly（OR=0.447，P=0.004） and eating meat（≥4 times/week：OR=0.293，P=0.001；1-3 times/week：OR=0.372，P=0.008） were significantly associated with a reduced risk for CHD. Conclusions：The first trimester is a critical period of fetal heart development. Mother old or young，exposuring to tobacco and catching a cold in early pregnancy are at increased risk for giving birth to a child with CHD；taking folic acid regularly and eating more meat are associated with a reduced risk for CHD.

Abstract:Objective：To evaluate the efficacy between deep hypothermic circulatory arrest（DHCA） and selective antegrade cerebral perfusion（SACP） used in pediatric aortic arch surgery. Methods：Sixty cases undergoing repair of coarctation of aorta or interrupted aortic arch complicated with cardiac monstrosity from January 2008 to March 2012 in our hospital were retrospectively reviewed. They were divided into two groups according to the method of cerebral protection：DHCA group（n=23） and SACP group（n=37）. Periopera－tive variables between the two groups were compared. Results：The lowest nasopharyngeal and rectal temperatures were significantly higher，postoperative intubation time was longer and thorax output during postoperative 24 h was larger in SACP group than in DHCA group（P<0.05）. There was no significant difference between two groups in terms of hospital mortality，occurrence of postoperative neurological complication，acute renal failure and low cardiac output syndrome（P >0.05）. Conclusions：Cerebral protective effect of DHCA and SACP in pediatric aortic arch surgery is comparable. SACP has the advantages of better recovery of respiratory function and less postoperative blooding.

Abstract:Objective：To observe the efficacy of entecavir combined with matrine in the treatment of HBeAg-positive related chronic hepatitis B. Methods：Totally 109 patients with HBeAg-positive related chronic hepatitis B were randomly divided into two groups.Patients in treatment group（59 cases） were treated by entecavir combined with matrine while those in control group（50 cases） were treated by entecavir singly. The treatment lasted for 48 weeks in every group. Hepatitis B two pairs of semi，serum HBV DNA repli－cation level and alanine transaminase（ALT） were detected respectively before treatment and at the 24th，48th week after treatment. Quantitative data were analyzed by t-test and rank sum test while qualitative data by chi-square test. Results：HBeAg clearance rate，HBeAg/anti-HBe seroconversion，HBV DNA recovery rate were significantly higher in treatment group than in control group at the 24th week after treatment，with significant differences between two groups（P values were 0.021，0.044，0.042 respectively）. HBeAg clearance rate，HBeAg/anti-HBe seroconversion，HBV DNA recovery rate were significantly higher in treatment group than in control group at the 48th week after treatment，with significant differences between two groups（P values were 0.039，0.012，0.011 respective－ly）. There was no statistical difference in ALT normalization rate between two groups at the 24th，48th week treatment（P >0.05）. Conclusions：Entecavir combined with matrine is effective in the treatment of chronic hepatitis B and can significantly improve the biochemical indicators of patients.

Abstract:Objective：To assess the influences of thrombolytic therapy on heart rate turbulence（HRT） and heart rate variability（HRV） in patients with acute myocardial infarction（AMI）. Methods：Sixty-two patients with AMI underwent thrombolytic therapy were di－vided into recanalization group（n=38） and non-recanalization group（n=24） and another 20 AMI patients without recanalization were selected as control group. The 24 h ambulatory electrocardiogram recordings were taken within 1-2 weeks after onset of AMI. HRT pa－rameters，turbulence onset（TO） and turbulence slope（TS） were calculated and HRV analysis was performed using time domain pa－rameters standard diviation of NN intervals（SDNN），root mean square of sussessive differences（RMSSD） and percentage of differ－ences exceeding 50 ms between adjacent normal number of intervals（PNN50）（6.89±2.14） ms/RR vs. （2.46±1.90） ms/RR，（2.55±2.38） ms/RR，q=3.64，P=0.008；（118.62±27.13） ms vs. （82.56±30.47） ms，（85.62±27.79） ms，q=4.27，P=0.002；（27.23±9.72） ms vs. （17.03±8.47） ms，（16.72±8.68） ms，q=3.95，P=0.005；（8.64±2.36）% vs.（3.14±1.87）%，（2.96±1.70）%，q=4.73，P=0.001 respectively）. Results：HRV was markedly reduced while HRT was markedly blunted in non-recanalization group and control group than in recanalization group（P<0.01 for all）. There were correlations between vascular recanalization after recanalization and HRV，HRT parameters（TO：rs=-0.257；TS：rs=-0.490；SDNN：rs=0.273；RMSSD：rs=0.466；PNN50：rs=0.392；P≤0.001 for all）. Conclusions：Successful thrombolytic therapy can improve the parameters of HRV and HRT and protect heart autonomic nervous sys－tem function in patients with AMI so as to improve their prognosis.

Abstract:Objective：To monitor the levels and changes of serum soluble interleukin-6 receptor（sIL-6R） in renal allograft recipients and to clarify its clinical significances. Methods：Clinical and baseline data of all enrolled patients were recorded in detail and an－other 20 healthy volunteers were chosen as normal controls. Sequential monitoring of serum sIL-6R was conducted by ELISA tech－nique in 60 patients before and after renal allograft. Results：There was no difference in serum sIL-6R level between normal controls and patients before renal allograft. After renal allograft，levels of serum sIL-6R were firstly increased，reaching the peak on the 3rd d then were decreased after targeted treatment and were reduced to levels before renal allograft after two weeks（F=15.23，P=0.003）. Fur－ther study demonstrated that levels of serum sIL-6R were significantly increased in renal allograft recipients complicated with infection（LSD-t=7.32，P=0.004）. Conclusions：Sequential monitoring of immunologic marker，serum sIL-6R in renal allograft recipients can be used to judge the acute rejection reactions at early stage and the combined complications.

Abstract:Objective：To discuss the multislice CT（MSCT） findings and pathologic characteristics of pulmonary sclerosing hemangioma（PSH） in order to improve the diagnosis of this disease. Methods：Totally 19 cases of PSH confirmed by operation and histopathology were collected. All cases underwent plain and enhanced CT scan. Retrospective analysis of MSCT features of PSH was done，moreover，the rare but specific CT findings and correlated pathologic basis were discussed. Results：（1）Eighteen cases had isolated lesion and 1 case had 2 lesions，20 lesions in total；among the 20 lesions，11（57.9%） were located in peripheral and 9 in central lung. （2）All lesions were round or oval in shape with well-defined boundary. Lesions of four cases（21.1%） had slight lobulation and lesions of 3 cases（15.8%） had calcification like sand. （3）All lesions were enhanced progressively and persistently at different extents；14 cases（73.7%） showed ‘blood vessel marginating sign’，4 cases（21.1%） showed ‘air meniscus sign’ and 5 cases（26.3%） with round glass opacity around lesions，namely ‘halo sign’. （4）All cases received enhanced scan，5 cases showed marked homogeneous enhancement whereas 14 cases（73.7%） showed intense and patchy heterogeneous enhancement，which was associated with different tissue compo－nents and proportions in hemorrhagic area，papillary area，solid area，sclerotic area of lesions. Conclusions：PSH usually occurs in the middle-aged female patients and locates near lung hilar or lung periphery with isolated lesions round or oval in shape；intense and patchy heterogeneous enhancement of lesions can be seen after enhanced CT scan. ‘Blood vessel marinating sign’，‘halo sign’ and ‘air meniscus sign’ around lesions are highly indicative of PSH，however，pathological check is still needed.

Abstract:Objective：To compare the efficacy and safety of clevudine（CLV） and adefovir（ADV） treatment for chronic hepatitis B patients（CHB）. Methods：Totally 18 CHB patients enrolled from our hospital between April and May 2010 were treated with daily dose of CLV 30 mg（n=9，experiment group） or ADV 10 mg（n=9，control group） randomly for at least 48 weeks. Liver and renal functions were tested；serum HBV DNA and HBV markers were detected for all patients before treatment at the 4th，8th，12th，24th，36th and the 48th week during treatment，respectively. Adverse drug reactions were observed. Results：At the 48th week during treatment，me－dian log10 HBV DNA in two groups were 2.081±0.228 and 3.017±1.578，respectively（P=0.115）. Partial virological response rates were similar（88.89%） in the two groups（P=1.000）. Complete virological response rates were 77.78 and 66.67% in experimental and con－trol groups（P=1.000）. Normalization rates of alanine aminotransferase were 66.67 and 77.78% in experimental and control groups（P=1.000）. HBeAg seroconversion rates were 33.33% and 0.00% in experimental and control groups（P >0.05）. One patient in experi－ment group showed virologic breakthrough with rtL180M and rtM204I mutation at the 47th week during follow-up. Conclusions：CLV showed potent antiviral activity for CHB patients；its safety is similar to ADV.

Abstract:Objective：To compare the clinical effect of herpes zoster（HZ） treated by routine dose and high-dose valacyclovir（VCV） classical antiviral drugs. Methods：Research source comes from the patients with herpes zoster admitted to our department from June 2011 to February 2012. Based on the principle of voluntary，patients with onset time <5 d were treated by high-dose antiviral therapy（observation group） and those with onset time <5 d were treated by normal-dose antiviral therapy（control group A） and those with onset time ＞5 d were treated by high-dose antiviral therapy（control group B）. Clinical effects and incidences of postherpetic neuralgia（PHN） of herpes zoster in the three groups at the end of treatment were compared and analyzed. Results：Pain relieving time，cessa－tion time of new lesion，beginning time of skin lesion crusting，50% lesion crusting time and subsiding time of skin lesions were sig－nificantly shorter in observation group than in control groups（A and B） during the treatment of herpes zoster，with statistical signifi－cances（P<0.05）. There was no significant difference in incidences and pain degrees of PHN of herpes zoster within the first six months after treatment between observation group and control groups. Above factors were not compared between two control groups because of no comparability. Conclusions：In the treatment of herpes zoster，high-dose antiviral therapy at early stage should be advocated in the clinical treatment as it has significant treatment effects. But how to make PHN of herpes zoster occur less and lighter needs further clinical study.

Abstract:Objective：To observe the morphology of left ventricle and changes in myocardial perfusion of peripartum cardiomyopathy （PPCM） before and after treatment by 99mTc-MIBI SPE MPI. Methods：Twenty-seven female patients with PPCM averaged 19-38 year-old were enrolled. Treatment preventing cardiac failure and ventricular remodeling were performed on them. 99mTc-MIBI SPE MPI was applied at one month（（29.41±2.95） d） after the treatment. Eleven patients were followed-up for six months（（6.31±0.84）months）. Left ventricle was divided into 17 segments and semi quantitative assessment was performed to evaluate the morphology of left ventri－cle，perfusion defect range and changes in left ventricular segmental score before and after the treatment. Results：Left ventricular size was decreased in 23 patients（85%），perfusion defect range was decreased（P=0.001） and myocardial perfusion was increased in all left ventricular segments except basal segment of anterior wall and basal segment of posterior wall（Pmax=0.024） at early stage of treat－ment compared with those before treatment. Left ventricular size was normal in 7 patients（64%），perfusion defect range was further decreased in 11 patients（P=0.009） and myocardial perfusion was further increased（Pmax=0.048） after 6 months’ treatment compared with those before treatment. Conclusion：99mTc-MIBI SPE MPI can be used to assess treatment in patients with peripartum cardiomy－opathy.

Abstract:Objective：To explore the relationship between Glasgow coma scale（ＧCS） and CT image score in predicting clinical results and prognosis of craniocerebral injury. Methods：Consecutive cranial CT scan was carried out in 129 patients with craniocerebral injury. All patients were divided into three groups according to CT image scores：mild injury（≤5 points），moderate injury（6~10 points） and severe injury（≥11 points）. CT image scores were compared with GCS scores and clinical prognosis. Results：Differences between the two kinds of scores were not statistically significant（?X2=0.44，P=0.80）. All types of craniocerebral injury according to CT image scores were statistically related with clinical prognosis（ X2=4.69，Ｐ＝０.03）. Conclusions：Correlation between CT image scores and GCS scores in craniocerebral injury classification exists，which provides an important objective method to evaluate the clinical prognosis of craniocerebral injury.

Abstract:Objective：To investigate the correlative factors and pathological types of fungal rhinosinusitis（FRS）. Methods：Clinical data of 163 patients with FRS enrolled between June 2005 and March 2010 in our hospital were retrospectively analyzed. Correlative factors and clinical features of FRS were investigated by using the multiple factor Logistic regression analysis. Pathological types of 163 cases of FRS were classified by using Gomori methenamine silvers taining，which was special for fungi. Results：Main risk factors for FRS were：over 50-year-old，female，hemato nasal discharge，headache，calcified plaque in CT scan，diabetes respectively. Among163 cases of FRS，61 cases were chronic invasive and 102 cases were non-invasive. Positive rate of fungal culture in patients with FRS was 93.86% and in normal controls was 22.38%，with statistical differences between them（P<0.05）. Conclusions：FRS has lots of cor－relative factors. We should take different treatment schemes for different patients based on their pathological types and correlative fac－tors of FRS in order to improve the therapeutic effects for FRS.

Abstract:Objective：To investigate the clinical efficacy of three-dimensional conformal radiotherapy combined with XELOX（oxali－platin+capecitabine） program chemotherapy for local recurrence after esophagectomy of esophageal cancer. Methods：A total of 69 cas－es with local recurrence after esophagectomy were randomly divided into treatment and control groups. In treatment group，35 patients received concurrent three-dimensional conformal radiotherapy and XELOX program chemotherapy. In control group，34 patients re－ceived XELOX program chemotherapy. Results：There was no statistically significant difference in general information between treat－ment group and control group. Response rates（RR） of treatment group and control group were 85.7% and 61.8%，with statistically sig－nificant differences（c2=5.130，P=0.024）. One-year survival rates of treatment group and control group were 65.7% vs. 41.2%（c2=4176，P=0.041），and 2-year survival rates of two groups were 45.7% vs. 20.6 %（c2=4.900，P=0.027），with statistically significant differences between two groups. There was no statistically significant difference in incidences of nausea，vomiting and leukopenia，anemia and thrombocytopenia between treatment group and control group（c2=0.206，P=0.650；c2=0.008，P=0.930；c2=0.174，P=0.676；c2=1.052，P=0.305）. Conclusions：Three-dimensional conformal radiotherapy concurrent with XELOX program chemotherapy can im－prove recent efficacy and long-term survival of patients with local recurrence after esophagectomy of esophageal cancer and reduce the side reactions.

Abstract:Objective：To evaluate the efficacy and safety of combined use of solifenacin and tamsulosin in the treatment of benign prostatic hyperplasia（BPH） accompanied by overactive bladder（OAB）. Methods：Totally 105 cases of clinically diagnosed BPH with－out serious obstruction of urinary tract were selected and were randomly assigned to Ⅰ group（n=50） to receive 0.2 mg of tamsulosin once a day and Ⅱ group（n=55） to be treated with 0.2 mg of tamsulosin once a day plus 5 mg of solifenacin once a day at night，both for 12 weeks. Before and after the treatment，international prostate symptom score（IPSS），quality of life（QOL） score，overactive bladder symptom score（OABSS），maximum urinary flow rate（Qmax），24 h micturition frequency，24 h urinary urgency frequency，24 h uroclep－sia frequency and urinary retention times of patients were recorded and used to do evaluation. Results：After 12 weeks medication，the assessable cases were 97；vales of IPSS，QOL，Qmax and 24 h micturition frequency were improved significantly in both groups（P <0.05）. Besides，OABSS，frequent micturition，urinary frequency and uroclepsia in groupⅡ were respectively better than those in groupⅠ（（4.82±1.15 vs. 9.27±2.10），（3.31±0.18 vs. 6.82±2.15），（8.02±2.15 vs. 10.13±2.07），（0.50±0.13 vs. 2.03±0.87），P<0.05）. No significant difference was found between two groups in Qmax（15.81±2.56 vs. 16.04±3.26，P ＞0.05）. No case of acute uri－nary retention occurred in either group. Incidences of adverse reactions in both groups were 4.3% and 8.0% respectively without sig－nificant differences. Conclusions：Combination therapy with tamsulosin and solifenacin is effective and safe in relieving the symptoms of patients with BPH and OAB and improving patient’s quality of life greatly.