Abstract:Objective：To use 5 methods of summary receiver operating characteristic（SROC） to evaluate the diagnostic value of the total bile acid（TBA） in intrahepatic cholestasis of pregnancy（ＩＣＰ） based on the Ｍeta-analysis. Methods：Articles were identified from searches of PubMed，HighWire，Ovid，the Cochrane Library，Google scholar research，the Chinese National Knowledge Infras－tructure，WanFang Dissertation Database and Chinese Biological Medical. The retrieval time was conducted at May 2015． Bivariate random effect models：η on ξ，ξ on η，D on S，R&G（Rutter’s and Gatsonis），major axis，were used based on the information of sensitivity，specificity，etc. SAS MIXED and NLMIXED procedure was applied to build 5 kinds of model to evaluate the diagnostic value of total bile acid of intrahepatic cholestasis of pregnancy. Results：The results area under curve（AUC） of diagnostic evaluation showed that η on ξ：0.711，ξ on η：0.448，D on S：0.730，Rutter and Gatsonis（R&G）：0.801，major axis：0.703，which indicated the di－agnostic accuracy of TBA was moderate，and 5 technical methods results of AUC and SROC curve showed that the effect of R&G was superior to other methods in evaluating the accuracy of TBA than. Conclusion：TBA is accurate when diagnosing intrahepatic choles-tasis of pregnancy，but the efficacy of R&G needs further consideration.

Abstract:Objective：To investigate and analyze the current situation of global appendicitis clinical practice guidelines and to provide references for the development and formulation of our native appendicitis clinical guidelines normatively. Methods：Chinese and Eng－lish databases were searched，including MEDLINE，Embase，National Guideline Clearinghouse，Guidelines International Network，Na－tional Institute for Health and Clinical Excellence，WANFANG database，CNKI and CPGN（Chinese practice guideline network），and relative websites（from established to December 31，2015）. Then literature was selected according to the inclusion and exclusion cri－teria. Names of guidelines，years of publication，institutions，guideline develop methods and number of each references were descrip－tively analyzed. The internationally recognized guideline evaluation tool，appraisal of guidelines for research and evaluation（AGREE） was applied to assess the methodogical quality of guidelines. Results：①A total of 7 appendicitis guidelines were included. Publica－tion dated from 2007 to 2014. The topics of these guidelines cover screening，diagnosis，drug therapy and surgical therapy；guidelines included are all evidence-based guidelines. ②The average scores of six domains（scope and purpose，stakeholders involvement，rigor of development，clarity of presentation，applicability and editorial independence） involved in the included guidelines were 65.3%，38.63%，48.89%，78.3%，0.0%，14.29%，respectively. Conclusion：There is a growing trend that clinical practice guidelines are devel－oped based on evidence. However，the total quality of reporting and the methodological rigor of guidelines need further improvement.

Abstract:Objective：To evaluate the value of two interferon-γ releasing assays（IGRA） in differential diagnosis of intestinal tubercu－losis（ITB） and Crohn’s disease（CD）. Methods：Datebases of Embase，PubMed，EBM Reviews，CNKI，WanFang were searched for evaluating the value of IGRA in differential diagnosis of ITB and CD. The Meta-Disc was used to calculate the pooled sensitivity，specificity and 95% confidence interval（95%CI）. Summary receiver operating characteristic curve（SROC），area under curve（AUC） and Q* were used to evaluate the efficiency of the two methods. Results：Totally 12 articles were included，including 854 patients（ITB 367，CD 487）. The pooled sensitivity and specificity for T-SPOT TB were 0.89（95%CI=0.84 to 0.93） and 0.85（95%CI＝0.81 to 0.89）. The pooled sensitivity and specificity for Quantiferon-TB were 0.72（95%CI=0.64 to 0.79） and 0.92（95%CI=0.86 to 0.96）. The AUC of SROC for the two methods were 0.9450 and 0.9110，and the Q* were 0.883 9 and 0.843 2. There was no statistically difference between the two methods（P=0.164）. Conclusion：T-SPOT.TB and Quantiferon-TB are both effective ways for the differ－ential diagnosis of ITB and CD. But there was no statistically difference between the two methods.

Abstract:Objective：To evaluate the clinical effectiveness of ulinastation combined with Xuebijing for treating sepsis. Method：The database such as Cochrane library，PubMed，CBM，CNKI，VIP and WanFang Data were searched to collect randomized controlled trials（RCTs），and the relevant references of included studies were also retrieved. Studies were screened，data were extracted，and the methodological quality was assessed by two reviewers independently. Meta-analysis was conducted by using RevMan 5.2 software. Results：A total of 15 studies involving 1 222 participants（experimental group：509，control group：713） were included. The results showed that compared with the group of routine therapies and group of single administration of either Ulinastatin or Xuebijing，experi－mental group of Ulinastatin combined with Xuebijing was superior in the following aspects with significant differences：morality：RR=0.45，95%CI=0.35 to 0.59，7d APACHEⅡ：MD=-4.91，95%CI=-6.83 to -2.98；duration of mechanical ventilation：MD=-7.33，95%CI=-10.16 to -4.51，the average length of ICU stay：MD=-8.63，95%CI=-11.50 to -5.76. Conclusion：According to the domestic evidence，ulinastatin combined with Xuebijing for treating sepsis is superior to both the routine therapies and the single administration of either Ulinastatin or Xuebijing. It provides a new and prospective therapeutic method for sepsis. However，this con－clusion has to be further verified by large scale and double blinded RCTs.

Abstract:Objective：To assess the impact of nesiritide on renal function of patients with heart failure. Methods：All the relevant liter－atures were searched from PubMed，Embase，Cochrane Library，OVID，Wangfang，CNKI，VIP，CBM，etc. Papers of related journals and conferences were also manually searched. Then the quality of included studies and extracted data were assessed and Meta-analysis was conducted with the evaluation method recommended by the guide of Cochrane Systematic Review 5.1.0. Results：A total of 642 articles were retrieved，but only 28 randomized controlled trials were finally included. The Meta-analysis showed that there was no significant difference in rising serum creatinine（SCr），blood urea nitrogen（BUN），creatinine clearance rate（Clcr），Cystatin C between the nesiritide group and the contrast（the therapeutic dose of nitroglycerin，sodium nitrate，dobutamine，diuretics and blank）（SCr：WMD=1.97，95％CI=-1.41 to 5.34，P=0.250；BUN：WMD=-0.21，95％CI=-1.01 to 0.60，P=0.610；Clcr：WMD=4.22，95％CI=-2.67 to 11.11，P=0.230；Cystatin C：WMD=0.06，95％CI=-0.24 to 0.36，P=0.690）. Meanwhile nesiritide was better in improving glomerular filtration rate（GFR） than the contrast（WMD=12.40，95％CI=8.87 to 15.93，P=0.000）. Conclusion：Compared with the other commonly used drugs of heart failure treatment，nesiritide doesn’t significantly affect the renal function of patients with heart failure，instead of improving GFR. So it is one of the safe drugs in the treatment of heart failure.

Abstract:Objective：To systemically review the effects of allopurinol on heart systolic function in patients with chronic heart failure. Methods：Randomized controlled trials（RCTs） in electronic databases（PubMed，The Cochrane library，ISI，CBM，CNKI，VIP and WanFang Data） and article reference lists（until August 2014） were searched，The therapeutic effects of allopurinol on patients with chronic heart failure were reviewed using Ｍeta-analytical techniques. Mean difference（MD） or standard MD（SMD） and relevant 95% confidence interval（CI） for continuous variables，relative risk（RR） and relevant 95%CI for dichotomous data were calculated. The RevMan 5.3 software package was used for the Ｍeta-analysis. Results：A total of 21 RCTs with 1 384 chronic heart failure patients were included in the Ｍeta-analysis. The present Ｍeta-analysis showed that there was a significantly higher left ventricular ejection fraction（LVEF） in allopurinol arm than that in control arm（MD=5.83，95%CI=4.65 to 7.01，P=0.000）. Subgroup analysis consistently suggested that allopurinol can significantly improve the LVEF regardless of whether included patients suffered from hyperuricemia（MD=6.09，95%CI=4.73 to 7.45，P=0.000），or not（MD=4.49，95%CI=2.99 to 5.99，P=0.000） at baseline，respectively. Similarly，allop－urinol can also significantly decrease the plasma B-type natriuretic peptide（BNP）/N terminal pro-BNP（NT-proBNP） level than control group（SMD=-0.62，95%CI=-1.00 to -0.24，P=0.001）. Three RCTs reported the side effects of allopurinol，and there was no significant difference in incidence of the side effects between the two arms. Conclusion：The present Meta-analysis suggested that conventional treatment combined with allopurinol therapy for chronic heart failure is effective and safe. Due to the limitation of the quality of included studies，this conclusion should be further confirmed with more high quality and large sample studies.

Abstract:Objective：To compare the clinical effectiveness of coaxial microincision cataract surgery（C-MICS） versus conventional coaxial small-incision cataract surgery（C-SICS） in the treatment of cataract. Methods：Randomized controlled trials comparing C-MICS with C-SICS were identified by searching CNKI，MEDLINE，Embase（2004 to July，2014） and the Cochrane Library（Issue 7，2014）. Data were synthesized using RevMan software（release 5.3） provided by the Cochrane Collaboration. Results：Through strictly including and excluding standard，a total of 14 trials（1 283 eyes） were included for Meta analysis. There was no significant difference between two groups in effective phacoemulsification time（P=0.36），mean phacoemulsification power（P=0.19），and central corneal thickness change（the first day，P=0.30；the first week，P=0.36；the first month，P=0.50）. There was no significant difference in corneal endothelial cell count at the first month between two groups（P=0.07）. The naked vision was statistically significantly better in C-MICS group than in C-SICS group on the first day，first month and the third month after the operation（the first day，P=0.003；the first month，P=0.000；the third month，P=0.002）. The surgical induced astigmatism was significantly less in C-MICS group than in C-SICS group on the first day，the first week，the first month and the third month after the operation（the first day，P=0.000；the first week，P=0.000；the first month，P=0.000；the third month，P=0.000）. Conclusion:C-MICS is superior to C-SICS in the treatment of cataract.

Abstract:Objective：To investigate the association between ATP binding casette transporter A1（ABCA1） gene R1587K polymorphism and coronary heart disease susceptibility. Methods：Literature was retrieved in electronic databases covering PubMed，Web of Science，CNKI，WanFang and VIP up to Jun，2014 to collect the case-control studies on ABCA1 gene R1587K polymorphism and coronary heart disease susceptibility. The odds ratios（ORs） and 95% conidence intervals（CIs） were taken as the effect indexes，either the fixed or random effect model was applied to conduct Meta-analysis by Stata 11.0 software in heterozygote，homozygote，dominant，and recessive genetic models，respectively. Results：A total of 5 case-control studies were identified，involving 2 032 cases and 11 492 controls. The current Meta-analysis showed that the AB－CA1 gene R1587K polymorphism was significantly associated with coronary heart disease under homozygote and dominant genetic model（homozygote：OR=1.140，95%CI=1.028~1.266，POR=0.014；dominant：OR=1.124，95%CI=1.017~1.243，POR=0.022）. Subgroup analysis results by ethnicity were significantly associated with Caucasian. Conclusion：The current Meta-analysis suggests that ABCA1 gene R1587K polymorphism might be associated with coronary heart disease susceptibility in Asian. Further studies are needed to val－idate the above conclusion.

Abstract:Objective：To systematically evaluate the efficacy of pseudomonas aeruginosa-mannose sensitive（PA-MSHA） for tumor. Methods：Randomized controlled trials（RCTs） about the efficacy of PA-MSHA on tumor were retrieved in Pubmed，Sciverse，Cochrone Library，CNKI，CBM，VIP and WanFang databases up to November，2014. According to the inclusion and exclusion criteria，two reviewers independently screened the trials，extracted the data，assessed the quality，conducted the Meta-analysis with Revman 5.3 finally. Results：A total of 10 RCTs were eligible for the study including 611 cases. The pooled results showed that complete re－mission（CR）（RR=1.95，95%CI=1.37 to 2.79，P=0.000 2） and overall response rate（RR）（RR=1.95，95%CI=1.37 to 2.79，P=0.000 2）were increased and progression rate of disease（PD） RR=0.42，95%CI=0.24 to 0.71，P=0.001）was decreased in experiment groups，however，partial response rate（PR） and stability rate of disease（SD） were not different in both two groups. Meanwhile，the count of CD3（SMD=0.93，95%CI=0.16 to 1.70），P=0.02），NK cell（SMD=1.40，95%CI=0.26 to 2.53，P=0.02），IFN-γ（MD=16.92，95%CI=12.88 to 20.96，P=0.000）was higher in experiment group than in control group. There was no statically difference in the number of CD4，CD8 and between two groups. Karnofsky scores were also higher in experiment group than in control group. The pooled results of subgroup analysis indicated that CR（P=0.006），PD（P=0.04） and NK cell（P=0.000） were increased in experiment group in lung cancer patients. RR（P=0.01） and Karnofsky scores（P=0.000） were also increased in experiment group in breast cancer patients. The results also showed that neo-adjuvant chemother－apy with PA-MSHA could improve Karnofsky scores（P=0.000 2）. Chemotherapy with PA-MSHA could improve CR（P=0.000 2），PD（P=0.003） and Karnofsky scores（P=0.000），oppositely，there was no difference in PR（P=0.73） and SD（P=0.39） between the two groups. Conclusion：PA-MSHA can effectively increase treat the tumor and enhance host anti-tumor function. PA-MSHA should be widely used in tumor administration in clinical application.

Abstract:Objective：To compare the safety and efficacy of robot-assisted laparoscopic hysterectomy and traditional laparoscopic hys－terectomy． Methods：Databases including Pubmed，Cochrane Library，EMBASE，Medline，Wanfang，CNKI and VIP were searched． Randomized controlled trials（RCTs） and non-randomized controlled trials（NRCTs） focusing the comparison between robot-assisted laparoscopic hysterectomy and traditional laparoscopic hysterectomy were collected． All the literatures retrieved were screened accord－ing to preset standards，and the patients were divided into robotic group and laparoscopic group． A Meta analysis on safety and efficacy of two kinds of hysterectomy was carried out using the RevMan 5.3 software． Categorical variables were presented by odds ratio（OR） and 95％confidence interval（95％CI），continuous variables were presented by weighted mean difference（WMD） and 95％ confidence interval（95％CI）． Results：5 RCTs and 26 NRCTs including 4 235 patients undergo hysterectomy were screened out and there were 1870 patients in the robotic group and 2 365 patients in the laparoscopic group. Meta analysis suggest that compared with conventional laparoscopic surgery，lower rate of perioperative period complication（OR=0．71，95%CI=0.57 to 0.89，Z=2.97，P=0.003），shorter length of hospital stay（WMD=-0．42，95%CI=-0.65 to -0.20，Z=3.67，P=0.000 2），less operative blood loss（WMD=-81.04，95%CI=-104.50 to -57.58，Z=6.77，P=0.000），lower rate of conver－sion to laparotomy（OR=0.38，95%CI=0.25 to 0.58，Z=4.60，P=0.000） were observed in the robotic group．There were no significant dif－ferences in operation time（WMD=15.38，95%CI=-4.48 to 35.23，Z=1.52，P=0.130） and the number of lymph node dissected[pelvic lymph node group（WMD=-1.09，95%CI=-2.61 to 0.43，Z=1.40，P=0.160）；perivascular lymph node group（WMD=-0．19，95%CI=-2.93 to 2.56，Z=0.13，P=0.890）] between two groups． Subgroup analysis suggest that the robotic group has less operative blood loss（WMD= -88.78，95%CI=-98.03 to -79.52，Z=18.80，P=0.000），there were significant differences between two groups. Conclusion：compared with conventional laparoscopic surgery，the robotic group has lower rate of perioperative period complication，shorter length of hospital stay，less operative blood loss（especially suitable for patients with small uterus），lower rate of conversion to laparotomy，However，there were no significant differences in operation time and the number of lymph node dissected. Whereas，considering the quality of the original literatures with obvious heterogeneity is not high，the present conclusions demonstrate strength is limited.

Abstract:Objective：To assess the therapeutic effects of muscarinic antagonists combined with non drug therapy or overactive bladder（OAB） in adult female. Methods：Published randomized controlled trials（RCTs）on muscarinic antagonists combined with non drug therapy and muscarinic antagonists therapy in the treatment of overactive bladder（OAB） in adult female were searched from PubMed，Embase，Web of Science，CNKI，CBWMDisc，Wanfang，VIP and other databases before January 2015 according to pre-specified stan－dard. Studies were qualified according to Jadad scale，and were analyzed using Revman5.3. Results：Totally 7 articles（610 patients），including 4 articles about muscarinic antagonists combined with electrical stimulation，2 articles about muscarinic antagonists com－bined with behavior training，1 article about muscarinic antagonists combined with both above. The meta-analysis showed that：① There was no difference in the average daily frequency of urination（WMD=-0.60，95%CI＝-1.23 to 0.03，P=0.06） and frequency of urinary incontinence（SMD=-4.45，95%CI＝-12.77 to 3.86，P=0.29）. Frequency of urgency（WMD=-1.09，95%CI＝-2.15 to -0.03，P=0.04） significantly decreased and total clinical efficiency（RR=1.24，95%CI＝1.06 to 1.44，P=0.007） improved obviously in the mus－carinic antagonists combined with electrical stimulation group compared with those of control group. ②Efficacy of muscarinic antago－nists combined with behavior training group was better than control group in reducing the average daily frequency of urination（WMD=-0.67，95%CI=-1.14 to -0.20，P=0.005）. Conclusion：Compared with single muscarinic antagonists therapy，the muscarinic antagonists combined with non drug therapy can better improve the partial urinary symptoms in adult female patients with OAB，and improve the total clinical efficiency.

Abstract:Objective：To construct the adenovirus vector containing human activating transcription factor 6（ATF6） gene and to study the effects on the proliferation and apoptosis of SW1353 cells in the stress condition. Methods：The ATF6 gene and the siRNA se－quence targeting ATF6 gene were respectively cloned into the shuttle plasmid pAdTrack-cmv and pSES-HUS，and then were ho－mogenously recombined with the adenovirus backbone plasmid pAdEasy-l in Ecoli BJ5183 to generate the adenovirus vector pAd-ATF6 and pAd-ATF6 siRNA.The recombinant adenovirus vector was transfected into the 293 packing cells by lipofectamine-mediated transfection to amplify the recombinant adenovirus Ad-ATF6 and Ad-ATF6 siRNA. The SW1353 cells were infected with the aden－ovirus and the expression levels of ATF6 mRNA and protein were detected by RT-PCR and Western blot. The effects of the recombi－nant adenovirus on the proliferation and apoptosis of SW1353 in the stress condition were detected by FCM，MTT and Western blot. Results：The recombinant adenovirus Ad-ATF6 and Ad-ATF6 siRNA with high infection were successfully obtained. FCM analysis showed that under stress condition，the S phase of SW1353 cells in the Ad-ATF6 group was increased significantly（P=0.000），and the apoptosis rate was decreased significantly（P=0.000） as compared with those of control group. Meanwhile the results of siATF6 groups were on the control. The MTT results and Western blot results were in accordance with that of FCM. Conclusion：Under stress condition，ATF6 may promote the proliferation of SW1353 cells and suppress its apoptosis.

Abstract:Objective：To investigate the effect of colchicine on proliferation and cell cycle（G2）of human KFB. Methods：The primary human keloid-derived fibroblast were cultured by tissue. The proliferative behavior of KFB was studied after pre-treating with various concentrations of colchicines（1，2，4，8，16，32 μg/ml） by 4 methyl thiazolyl tetrazolium method and the cell cycle was assessed by flow cytometry. Results：MTT analysis showed that 1 μg/ml group（12.56±0.79）%，2 μg/ml group（27.35±0.50）%，4 μg/ml group（34.36±0.25）%，8 μg/ml group（39.38±0.57）%，16 μg/ml group（40.38±0.23）%，32 μg/ml group（44.65±0.60）%，F=2440.178，P=0.000；flow cytometry analysis showed that blank control group（0.67±0.46）%，1 μg/ml group（4.26±1.51）%，2 μg/ml group（24.51±4.69）%，4 μg/ml group（45.12±2.75）%，8 μg/ml group（55.81±5.04）%，16 μg/ml group（69.90±3.61）%，32 μg/ml group（85.53±2.06）%，F=491.609，P=0.000. Not only was the proliferation of KFB was inhibited，but also the prophase of cell division（G2） was af－fected depending on the time and drug concentration. Conclusion：The primary human keloid-derived fibroblast should be cultured by tissue adherent culture method and colchicine may regulate the proliferation and prophase of cell division of KFB at 1-32 μg/ml in 24 hour.

Abstract:Objective：To investigate the effect of bifidobacterium infantis（BI）-mediated herpes simplex virus thymidine kinase（HSV-TK）/ganciclovir（GCV） suicide gene therapy system on cell apoptosis of prostate cancer PC-3 cell xenografts in nude mice. Methods：The prostate cancer model was established by subcutaneous injection of human prostate cancer cell line PC-3 into nude mice. The model mice were randomly divided into 4 groups，which were treated by intraperitoneal injection of GCV in combination with tail vein injection of BI carrying recombinant plasmid BI-pGEX-TK（recombinant plasmid group），BI carrying empty plasmid BI-pGEX-5x-1（empty plasmid group），BI（BI group） and normal saline（normal saline group）. After 4 weeks of treatment，tumor weights and nude mice weights were recorded. The apoptosis of tumor was detected by TUNEL assay. The expression of Fas，FAP-1，casepase3 and casepase8 were determined by real-time qPCR and Western blot，respectively. Results：After treatment，comparing with those of empty plasmid group，BI group and normal saline group，the nude mice weights in recombinant plasmid group increased（F=12.952，P=0.000），the tumor weights decreased（F=7.795，P=0.000）. The apoptosis index of recombinant plasmid group was obviously higher than that of the other three groups（F=45.895，P=0.000）. Comparing with the other three groups，the mRNA expression of Fas（F=70.077，P=0.000），casepase3（F=70.611，P=0.000） and casepase8（F=73.781，P=0.000） in recombinant plasmid group increased，but the mRNA expression of FAP-1（F=46.880，P=0.000） decreased. Protein expression of Fas（F=664.116，P=0.000），casepase3 F=203.090，P=0.000） and casepase8（F=654.354，P=0.000） in recombinant plasmid group increased，but the expression of FAP-1 protein（F=318.989，P=0.000） decreased. Conclusion：The BI-mediated HSV-TK/GCV suicide gene therapy system can inhibit nude mice prostate cancer progression effectively，probably through affecting Fas mediated apoptosis signaling pathways，inducing to nude mice prostate cancer cells apoptosis and playing a role in tumor suppression.

Abstract:Objective：To observe the protective effect of Yulangsan polysaecharide（YLSPS） on diclofenac-induced liver injury in Kunming mice. Methods：The models of diclofenac-induced liver injury in mice with method of injection were used to study the pro－tective effect of YLSPS. Kunming mice were randomly divided into liver injured DICF group，NC group，YLSPS high-，middle-，low- group（600，300，150 mg/kg） and dimethyl diphenyl bicarboxylate（200 mg/kg DDB） group. The treatment groups were orally adminis－tered once per day whereas the normal and model groups were orally administered with saline for 14 d. Except normal group，all the other mice were injected intraperitoneally with DICF（50 mg/kg body weight）. After fasting for 20 h，animals were sacrificed and blood，liver samples were obtained. The levels of alanine aminotransferase（ALT），aspartate aminotransferase（AST），alkaline phos－phatase（ALP），total bilirubin（TBIL），tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） in serum and levels of superoxide dismutase（SOD），glutathione peroxidase（GSH-Px），catalase（CAT），malondialdehyde（MDA） in liver tissue were measured. Hema－toxylineosin（HE） stain was used to examine the degree of hepatic injury. Results：Compared with model group，YLSPS could reduce the levels of ALT，AST，ALP，TBIL，MDA，TNF-α and IL-1β. The activities of GSH-Px，SOD，and CAT could be increased （P<0.05），and the degree of hepatic injury could be lessened. Conclusion：YLSPS has protective effect on diclofenac-induced liver injury in mice. The mechanism may be related to attenuat－ing free radical and inhibiting the effect on lipid peroxidation.

Abstract:Objective：To explore the effect of cytokine induced killer（CIK） on the growth and proliferation of liver cancer stem cells （LCSCs）. Methods：LCSCs were prepared from liver cancer cell line SMMC7721 induced by serum-free medium（SFM） containing several cytokines（LIF，EGF，bFGF and B27），and their CD90 and CD133 were identified by flow cytometry（FCM）. Meanwhile，CIK cells were produced from suspended peripheral blood mononuclear cells（PBMCs） of patients with hepatocellular carcinoma（HCC） in－duced by IFN-γ，human CD3 monoclonal antibody（hCD3mAb） and IL-2. LCSCs and CIK were cultured in a same circumstance with a Transwell culturing cabin，but the LCSCs were cultured at one side of the membrane of the Transwell，and the CIK cells were cul－tured at another side of the same membrane for 24 h and 48 h respectively. The effects of CIK cells on the growth and proliferation of LCSCs were detected by cell count kit-8（CCK-8）. The expression of gene and encoding protein of proliferating cell nuclear antigen （PCNA） were assayed via RT-PCR and Western blot respectively. Results：The high expressions of CD90 and CD133 in LCSCs were tested by FCM. The results of CCK-8 indicated that the growth and proliferation of LCSCs co-cultured with the CIK cells were slower than those of control cells，which become more visible at 48 h after co-culturing（P<0.01）. The results of RT-PCR and Western blot revealed that CIK cells can significantly down-regulate the ex－pression of gene and encode protein of PCNA in LCSCs. Con－clusion：LCSCs are induced，cultured and identified successfully. The CIK cells induced from patients with HCC can significantly prevent LCSCs from growth and proliferation and down-regulate the gene and encode protein of PCNA in them.

Abstract:Objectives：To study the expression and distribution of SGK1 in preeclampsia placenta tissues，and to explore the effects of hypoxia on the expression of SGK1 in placental trophoblast cells. Methods：Twenty-five cases of normal pregnancies，mild pre-eclampsia and severe preeclampsia were chosen，and all of these cases were delivered by cesarean section in The Second Affiliated Hospital of Chongqing Medical University from 2012 December to 2013 November. The expression level of SGK1 in placenta was detected by immunohistochemistry and Western blot. HTR-8/SVneo cells were cultured in normoxia conditions（21% O2、5% CO2） and hypoxic conditions（1% O2、5% CO2）. The expression level of SGK1 in human trophoblast cells was detected by Western blot. Results：lmmunohistochemistry revealed that the expression level of SGK1 in mild preeclampsia group and severe preeclampsia group was higher than that in control group（P=0.000）. The expression level of SGK1 in severe preeclampsia group was higher than that in mild pre-eclampsia group（P=0.028）. Western blot revealed that the protein expression level of SGK1 in mild pre-eclampsia group and severe preeclampsia group was higher than that in control group（P=0.000）. The protein expression level of SGK1 in severe preeclamp－sia was higher than that in mild pre-eclampsia group（P=0.000）. Masson staining revealed that normal placenta tissue existed fibrosis，and the extent of fibrosis was increased in mild pre-eclampsia group and severe preeclampsia group（P=0.000）. The extent of fibrosis in severe preeclampsia was higher than mild pre-eclamp－sia group（P=0.000）. In vitro，Western blot revealed that hypoxia increased SGK1 and CTCF transcription in HTR-8/SVneo cells（P=0.000；P=0.001），and masson staining revealed that hypoxia increased the production of collagen fibers（P=0.000）. Conclusion：Expression of SGK1 in preeclampsia placenta tissue is significantly increased，suggesting that SGK1 may play an important role in the pathogenesis of preeclampsia，hypoxia increased by stimulating the expression of SGK1，involved in preeclampsia.

Abstract:Objective：To investigate the association of single nucleotide polymorphism（SNP rs325400 and rs34851361） of MEF2A gene on chromosome 15q26 with coronary artery disease（CAD）. Methods：A case-control association study of 90 unrelated CAD patients and 130 normal controls from a Chinese Han population was performed. Genotype and allele were determined by polymerase chain reaction（PCR） and nucleotide sequencing for SNP rs325400 and rs34851361 in MEF2A gene. Results：The frequencies of the geno－type and alleles of rs325400 in MEF2A had statistical significance between two groups（P＜0.05），but there was only the AA in both groups of rs34851361. Conclusion：The present findings suggest that the genetic polymorphism in rs325400 may be associated with the development of the CAD in Chinese Han population and the attack rate of the CAD in the crowd with allele A may be higher.

Abstract:Objective：To investigate the distribution of the subtype of Treponema pallidum（TP） in Chongqing，and to explore the clini－cal correlation of genetype. Methods：Four hundred twenty one specimens were collected from patients with seropositive syphilis from June 2010 to October 2013，and were screened by nested PCR targeted TP DNA polymerase I gene（polA）. The acid repeat pro－tein（arp），treponema pallidum repeat（tpr） and TP0548 genes were amplified with the positive specimens above. Then the strains were subtyped according to Pillay’s criteria and subtyping method of targeted three genes. Analysis was made concerning the correla－tion of different genetypes with sero-resistance，syphilis recurrence and Jarisch-Herxheimer reaction by Fisher’s exact test，respec－tively. Results：Totally 163 specimens were positive by PCR targeted polA gene. Of 116 TP positive specimens subtyped by arp，sub－type 14 was the predominate genotype，while of 114 specimens subtyped by tpr gene，d was the most common genotype. Of 117 specimens subtyped by TP0548 gene，subtype f was predominate. At 12 months after standard treatment，32 patients occurred sero-re－sistance including 11 subtype d，2 subtype b，2 subtype a and 1 subtype f. Three patients experienced syphilis recurrence including 2 TP0548 subtype c and 1 subtype f. Eight patients developed Jarisch-Herxheimer reaction，including 4 genotype 14f/f，3 geno－type 14d/f and 1 patient failing to subtype. There were statistical differences in the correlation of molecular subtypes with sero-resis－tance and Jarisch-Herxheimer reaction（P=0.000，P=0.029），while no statistical difference of the correlation of genetypes with syphilis recurrence was found（P=0.333）. Conclusion：The predominate subtypes of TP in Chongqing is subtype 14，d and f. It is suggested that the sero-resistance be related with TP tpr subtype b and the second satge syphilis with 14f/f is more likely to develop a Jarisch-Herx－heimer reaction following benzathine penicillin treatment.

Abstract:Objective：To investigate the effect of thiostrepton（TST） on the apoptosis of nasopharyngeal carcinoma CNE-2 cell line. Methods：CNE-2 cells were delivered into a control group and a group treated with 4 μmol/L or 8 μmol/L of TST for 48 h. Then CNE-2 cell morphology was observed by inverted phase contrast microscope. The apoptosis rate was analyzed by Hoechst33342 and flow cytometry. Expressions of FoxM1 protein and apoptosis protein were detected by Western blot. Results：CNE-2 cells underwent dra－matic changes in morphology after being treated with different concentrations of TST. Hoechst33342 assay showed that exposure to TST resulted in dose-dependent increase of CNE-2 cell apoptosis. Apoptosis cell numbers of control group，4 μmol/L TST group，8 μmol/L TST group were 0.60±0.55，3.60±0.89，7.00±1.58. Flow cytometry assay indicated that the apoptosis rate was increased with the increase of TST concentration；apotosis pencentages of control group，4 μmol/L TST group，8 μmol/L TST group were （1.87±0.74）%，（10.55±0.75）%，（19.35±2.49）%. Western blot showed that the expression levels of FoxM1 protein and apoptosis associated proteins decreased with the increase of TST concentration. Conclusion：TST can effectively inhibit CNE-2 cell proliferation and induce apoptosis in nasopharyngeal carcinoma.