Abstract:Objective：Chemokine receptor CXCL12 is a small protein that mediates cell chemotaxis. Previous studies confirmed that chemokine receptor CXCL12 was a principal regulator of homing，migration and mobilization of haematopoietic stem cells and endothelial pro－genitor cells（EPCs） during steady-state homeostasis and injury. Once previously thought to interact exclusively with CXCR4，CXCL12 also binds to CXCR7（recently renamed ACK3R），which belongs to an atypical chemokine receptor family whose members fail to ac－tivate G proteins but interact with β-arrestins，control the CXCL12 bioavailability，enhance or dampen CXCR4- mediated signaling and activity.Here we discuss the role of CXCR7 in cell proliferation，angiogenesis，and metastasis，with a focus on cardiovascular dis－ease.

Abstract:coronary heart disease；coronary flow reserve；transthoracic echocardiography

Abstract:Objective：To evaluate the effect of simvastatin combined with ezetimibe on testosterone values in male patients with stable coronary artery disease（SCAD） and without diabetes；to observe the blood glucose level after the combined therapy. Methods：A sin－gle-center，randomized，open-label trial involving 90 patients was conducted. The patients were randomized into two groups：the trial group received 40 mg per day of simvastatin plus 10 mg per day of ezetimibe and the control group received simvastatin 40 mg per day alone；the treatment in both groups lasted for 12 weeks. Testosterone，fasting blood glucose（FBG），glycosylated hemoglobin A1c（HbA1c），lipid profiles were determined at the enrollment and at the end of the treatment. Results：Both treatments were equally effective in increasing total cholesterol（TC） and low-density lipoprotein-cholesterol（LDL-C）（P＜0.05）. TC and LDL-C levels were lowered in trial group than in control group（P＜0.05）. At the end of study，testosterone levels remained unchanged in control group and slightly decreased in trial group（P=0.085）. No explicit elevation in FBG or HbA1c was observed in both groups after the treatment. Safety and tolerability profiles were similar in the two groups. Conclusion：Simvastatin 40 mg combined with ezetimibe 10 mg can reduce LDL-C without affecting testosterone levels. No side effect in glycometabolism is observed in the short term.

Abstract:Objective：To investigate the effects of ghrelin on myocardial remodeling matrix metalloproteinases expression in post-my－ocardial infarction diabetic rats. Methods：Adult male SD rats were divided into four groups：myocardiac infarction（ＭＩ），diabetes mellitus+myocardiac infarction（DM+MI），DM+MI+ghrelin，DM+MI+ghrelin+GHSR1a inhibitor[D-Lys3]-GHRP-6. Diabetes was in－duced by injection of streptozotocin（STZ，60 mg/kg）. After three months，left anterior descending artery（LAD） ligation was performed in all groups. DM+MI+ghrelin group received ghrelin，200 ?滋g/（kg·d）. DM+MI+ghrelin+[D-Lys3]-GHRP-6 group received ghrelin， 200 ?滋g/（kg·d） and [D-Lys3]-GHRP-6，50 mg/（kg·d）. The other two groups received the same amount sterile normal saline only. Four weeks later，Sirius red staining was used to detect myocardial fibrosis. Myocardial infarct size was detected by Masson staining. The distribution and expressions of matrix metalloptoteinase-2（MMP-2），matrix metalloptoteinase-9（MMP-9） and tissue inhibitor of metalloprotroteinase-1（TIMP-1） were detected by immunohis－tochemistry and Western blot，separately. Results：Myocardial collagen volume fraction（P=0.003），myocardial infarct size（P=0.001），the expression of MMP-2（P=0.000） and MMP-9（P=0.000） were significantly increased in DM+MI group than in MI group. After ghrelin administration，myocardial collagen volume fraction（P=0.001），myocardial infarct size（P=0.005），the expres－sion of MMP-2（P=0.001） and MMP-9（P=0.001） were significantly decreased in diabetic rats complicated with MI. TIMP-1 was sig－nificantly higher in diabetic groups than in MI group（P=0.000），without significant difference among these groups（P ＞0.05）. However，[D-Lys3]-GHRP-6 blocked the above effects of ghrelin. Conclusion：Ghrelin can inhibit myocardial extracellular matrix remodeling and reduce myocardial infarct size by suppressing the expressions of MMP-2 and MMP-9.

Abstract:Objective：To establish hypoxia drug resistance model（HDRM） of human ovarian cancer cell lines A2780 and SKOV3 by chemical induction method of Cobalt Chloride（CoCl2） and to compare the drug resistant characteristics of those two cell lines. Methods：CoCl2 with different concentrations（0，50，100，150，200，300 μmol/L） was added to A2780 and SKOV3 cells for 12，24，48，72 h. Cell proliferation activity（CPA） was detected by methyl thiazolyl tetrazolium（MTT）. MTT was used to detect the drug resistance multiple（DRM） of paclitaxel under the different concentrations of CoCl2. The same cell lines without CoCl2 were taken as normoxic control. CoCl2 with concentration of 150 μmol/L was added to A2780 and SKOV3 cells medium for 24 h respectively and the expression of HIF-1α mRNA was detected by the real time polymerase chain reaction. The same cell lines without CoCl2 were taken as normoxic control. Results：The proliferation activity of A2780 and SKOV3 cells was correlated with CoCl2 in a dose and time dependent manner（A2780 group：F=1 165.416，P＝0.000；SKOV3 group：F=2 802.394，P＝0.000）. Paclitaxel resistance fold of those two kinds of cells was increased with the increase of CoCl2 concentration（F=7 842.711，P＝0.000）. With the same concentration of CoCl2，DRM of SKOV3 cells was significantly higher than that of A2780 cells（50 μmol/L group：t=-48.287，P＝0.000；100 μmol/L group：t=-263.205，P＝0.000；150 μmol/L group：t=-143.305，P＝0.000）. With 150 μmol/L CoCl2 culturing for 24 h，DRM of A2780 and SKOV3 cells reached to 9.84±0.11 and 21.08±0.08，respectively. The increase of HIF-1α mRNA expression in SKOV3 cells was significantly higher than that of A2780 cells（t=-5.573，P＝0.000）. Conclusion：Ovarian cancer HDRM can be successfully established by CoCl2 induction method and DRM is related with cell characteristics. Under the same condition，SKOV3 cell line is easier to establish HDRM than A2780 cell line，and SKOV3 is an ideal cell line for hypoxia resistance reversal study.

Abstract:Objective：To explore the ERK1/2 expressions and significances in hypoxia/reoxygenation cardiomyocytes post-processed by H2S. Methods：H9C2 cardiomyocytes were exposed to hypoxia/reoxygenation and then post－processed by H2S. The cardiomyocytes were divided into five groups：hypoxia/reoxygenation group（H/R），hydrogen sulfide post-treatment group（H2S），hydrogen sulfide post-treatment with ERK inhibitor group（H2S+PD），ERK inhibitor group（PD） and control group（control）. The expressions of ERK1/2 mRNA were detected by qPCR，and ERK1/2 protein expressions were detected by Western blot. In each group the proliferation rates of cardiomyocytes were analyzed by CCK-8，and the cell apoptosis rates were analyzed by flow cytometry. Results：There were signifi－cantly statistical differences in expression of ERK1/2 mRNA gene among H2S group，H/R group，H2S+PD group and PD group（F=23.000，P=0.000）. ERK1/2 mRNA was over expressed in rat H9C2 cells in H2S group（P=0.005），more evident in H2S+PD group（0.895±0.106） than in H/R group（P=0.027）. There were significantly statistical differences in expression of ERK1/2 protein among H2S group，H/R group，H2S+PD group and PD group（F1=5.15，P1=0.016，F2=5.44，P2=0.045）. CCK-8 assay showed that of myocardial cell proliferation was significantly increased in H2S group than in the other groups. Flow cytometry results suggested that myocardial cell apoptosis rate was significantly lower in H2S group than in the other groups. Conclusion：ERK1/2 mRNA and protein expressions of hypoxia/reoxygenation cardiomyocytes are significantly increased，with higher proliferation rate and lower apoptosis rate after H2S treatment. Exogenous donor H2S could activate ERK signal pathway，further benefiting the recovery of hypoxia/reoxygena－tion cardiomyocytes and leading to myocardial protection.

Abstract:Objective：To investigate the effect of different doses of statins on blood lipid levels and safety of young and middle-aged coronary heart disease patients after coronary stent implantation. Methods：Retrospective analysis on the young and middle-aged pa－tients who had coronary stent implantation in our department from December 2013 to June 2014 was conducted. Totally 81 patients received rosuvastatin at the dose of 10 mg/d and 87 received rosuvastatin at the dose of 20 mg/d. After the treatment of 6 to 8 weeks，the changes of levels of total cholesterol（TC），triglyceride（TG），low density lipoprotein cholesterol（LDL-C），high density lipoprotein cholesterol（HDL-C），alanine transaminase（ALT），aspartate transaminase（AST），hypersensitive3 C-reactive protein（hs-CRP），Crea－tine Kinase（CK）and adverse reaction rate were compared between the two groups before and after treatment. Results：After the treat－ment of 6 to 8 weeks，significant declines were found in the levels of TC，TG，LDL-C，hs-CRP both in the two groups. HDL-C was significantly higher after the treatment. The changes in the levels of TC LDL-C and hs-CRP in high-intensity rosuvastatin group were significantly higher than those of moderate-intensity rosuvastatin group，with statistical significances. No patient experienced the ab－normal transaminase and abnormal muscle enzymes. Conclusion：For the young and middle-aged patients after coronary stent im－plantation，high-intensity statin（20 mg rosuvastatin） is more effective and safe than moderate-intensity statin（10 mg rosuvastatin），and has good safety.

Abstract:Objective：To evaluate the clinical efficacy of percutaneous balloon pulmonary valvuloplasty（PBPV） for pulmonary stenosis（PS） complicated with subvalvular pulmonary stenosis（mild） and supravalvular pulmonary stenosis. Methods：A total of 45 patients of pulmonary stenosis complicated with subvalvular pulmonary stenosis（mild） and supravalvular pulmonary stenosis，who encountered in author’s hospital during past 10 years，were enrolled in this study. The diagnosis was conformed by clinical manifestations，electro－cardiograph（ECG），X-ray，echocardiography. The valve ring size，valvular area，pulmonary valve differential pressure were confirmed before the PBPV. The balloon with the diameter（20％-40％） bigger than the valve ring was confirmed. After the PBPV，the PA-RV （pulmonary artery-right ventricle） pressure was measured immediately. The pulmonary valve open，ECG，pulmonary valve and tricus－pid valve regurgitation were checked. Results：Among 45 cases of pulmonary stenosis complicated with subvalvular pulmonary stenosis （mild） and supravalvular pulmonary stenosis，42 cases had successful intervention，with the success rate of 93.333％. In the pul－monary stenosis complicated with subvalvular pulmonary stenosis（mild） and supravalvular pulmonary stenosis group，preoperative and postoperative pulmonary transvalvular pressure gradient was statistically significant（P＜0.05）. In pulmonary stenosis complicated with subvalvular pulmonary stenosis（mild） and supravalvular pulmonary stenosis，preoperative and postoperative pulmonary transvalvular pressure gradient was statistically significant（P＜0.05）. Conclusion：When the blood flow velocity of right ventricular outflow tract is less than 3.810 m/s，PBPV is the effective treatment for subvalvular pulmonary stenosis（mild）. PBPV is safe and effective for pulmonary stenosis complicated with supravalvular pulmonary stenosis（the diameter of supravalvular pulmonary stenosis is larger than 5 mm）. In pulmonary stenosis complicated with subvalvular pulmonary stenosis （mild） and supravalvular pulmonary stenosis，PBPV is effective when the pulmonary valve ring is well developed or mild-to-moderate maldeveloped.

Abstract:Objective：To evaluate the effect of angiotensin enzyme inhibitors（ACEI） and angiotensin receptor blockers（ARB） therapy on contrast-induced nephropathy（CIN） in patients undergoing coronary angiogram（CAG） or percutaneous coronary intervention （PCI）. Methods：Search was conducted in PubMed，The Cochrane library，VIP，CNKI and Wanfang data for literatures about ACEI or ARB and CIN up to August 2014. According to the inclusion and exclusion criteria，articles were screened and data were extracted and quality of included studies was evaluated. Mata analysis was conducted by using Stata 11.0 software. Moreover，Begg’s and Egger’s regression were used to assess the publication bias of included articles. Results：Eleven studies were included. Among the patients un－dergoing PCI or CAG，the usage of ACEI/ARB before those operations did not increase the risk of CIN compared with that of control group，and there is no significant difference（RR=0.78；95%CI＝0.53 to 1.15，P=0.22）. In subgroup analysis，subgroups underwent ACEI/ARB treatment for at least 2 weeks（RR=0.78，95%CI＝0.37 to 1.62，P=0.50） and less than 2 weeks（RR=0.79，95%CI=0.50 to 1.24，P=0.30） had no influence on the incidence of CIN. The risk of CIN at 72 h after coronary intervention therapy was decreased（RR=0.23，95%CI=0.23 to 0.87，P=0.02） and the subgroup with contrast agent≤150ml showed a decreasing incidence of CIN（RR=0.57，95%CI=0.33 to 0.99，P=0.046）. Conclusion：There is no evi－dence to suggest that use of ACEI/ARB will increase the risk of CIN in patients undergoing PCI/CAG at present.

Abstract:Objective：To evaluate the effectiveness and safety of point application therapy in treating table angina pectoris（SAP）. Methods：An extensive search of 4 English medical databases（PubMed，CochraneLibrary，Clinicaltrials，MEDLINE），and 4 Chinese medical databases（WanFang，CBM，CNKI，VIP） was conducted. Randomized controlled trials（RCTs） in accordance with inclusion and exclusion criteria were included. And quality of enrolled studies was assessed. A Meta-analysis was performed by Review Manager 5.2 software. Results：After researching，a total of 9 RCTs were included. Only one of the included RCTs had a clear random method. The allocation concealment and blinding of all included studies were not sure. There were statistical differences in relieving angina symptoms between point application therapy group and drug therapy group（RR=1.13，95％CI=1.07-1.20，P=0.000）. There were statis－tical differences in ECG effect between point application group and western medicine group（RR=1.12，95％CI=1.04-1.20，P=0.002）. There were statistical differences in traditional Chinese medicine（TCM） syndrome between two groups（RR=1.14，95％CI=1.05-1.25，P=0.004）. In addition，3 trials reported adverse effects such as skin rash，gastrointestinal adverse reactions. Conclusion：The point application therapy appears to have beneficial effects on relieving angina symptoms and TCM syndromes in participants with SAP. However，the findings should be interpreted with caution due to the poor methodological quality and small sample of the included trials.

Abstract:Objective：To evaluate the association between SLCO1B1 gene 521 T>C polymorphism and Statins effectiveness among Chinese population. Methods：Databases including PubMed，Web of Science，Embase，Cochrance Library，CNKI，WanFang Data and VIP were searched to retrieve the relevant references from the start to June 2014. A quality assessment was performed using the Methodological Index for Non-Randomized Studies（MINORS） criteria. Meta-analysis，sensitivity analysis（Revman 5.2），publication bias measuring were all done（Stata 12.0）. Results：A total of 5 studies were included for final meta-analysis，involving 812 partici－pants. Overall，there was no statistically significant association for the four genetic models of hypolipidemic effect. The dominant ge－netic model TC + CC vs. TT（MD=-0.38，95%CI=-1.91 to 1.14，P=0.62），recessive model CC vs. TT + TC（MD=0.09，95%CI=-1.77 to 1.96，P=0.92），the additive genetic model CC vs. TT（MD=-0.03，95%CI=-2.34 to 2.28，P=0.98） and TC vs. TT（MD=0.00，95%CI= -1.95 to 1.95，P=1.00）. Conclusion：There is no association between SLCO1B1 521 T>C polymorphism and Statins effectiveness among Chinese population.

Abstract:Objective：To investigate the cytogenetic abnormalities of patients with plasma cell dyscrasias by fluorescence in situ hy－bridization（FISH） in Chongqing region and its clinical significance. Methods：Panel probes of D13S319/RB1，CCND1/IgH，p53 and CKS1B/CDKN2C were used to detect the chromosomal abnormalities in 50 patients with plasma cell dyscrasias. At same time the con－ventional chromosome check was done. The correlation of cytogenetic abnormalities with clinical feature was studied. Results：Molec－ular genetics change was found in 34 cases（68%） out of 50 cases. One kind of abnormity was found in 24 cases（48%） and two or more than two kinds of abnormity was found in 10 cases（20%）. The ratio of abnormity form high to low was as followed：IgH gene aber－rance（36%），13q deletion（24%） and P53 gene deletion（20%）. Only 6 cases（12%） were found chromosome structural abnormalities by conventional chromosome check. The genetics abnormality was not correlated with age，gender，subgroup and stage. Conclusion：FISH method can more easily find abnormity than conventional chromosome check. High incidence of IgH gene aberrance，13q and P53 gene deletion is find among patients with plasma cell dyscrasias in Chongqing region，nevertheless，having no association with recent prognosis.

Abstract:Myelodysplastic syndromes（MDS） is originated from pluripotent hematopoietic stem/ progenitor cell with heterogeneity ma－lignancies and the pathogenesis is still obscure. Interstitial deletion of chromosome 5 is the most common cytogenetic abnormality in MDS and it is related with the pathogenesis of MDS. Furthermore，haploinsufficiency in RPS14，SPARC and the absence of mutations in miRNA，P53 were found in del（5q）MDS patients and RPS14 was proved to play the anti-oncogene role in the 5q-syndrome. This article summarized the newly released investigation of molecular pathogenesis associated with the development of MDS with del（5q）.

Abstract:Objective：To observe the changes of glceraldehyde-3-phosphate dehydrogenase（GAPDH） and β-actin gene expression during bone marrow mesenchymal stem cells（MSCs） differentiation to the osteogenic lineage induced by bone morphogenetic proteins 9 in order to select more suitable reference genes. Methods：Alkaline phosphatase（ALP） was used as a marker to identify BMP9-in－duced MSCs’ osteogenic differentiation；and mRNA and protein of GAPDH and β-actin were detected by real-time PCR and West－ern blot respectively on 0，1，3，5，7 d during MSCs’ osteogenic differentiation. Results：（1）mRNA expression of β-actin was signifi－cantly increased during osteogenic differentiation；there were significant differences in mRNA expression of β-actin between d0 and d5；no apparent change was observed in GAPDH. Results showed that the protein level of β-actin was increased gradually espe－cially on d5 and d7，while no apparent change was observed in GAPDH. Conclusion：During BMP9 induced MSCs’ osteogenic dif－ferentiation，GAPDH is more suitable than β-actin as internal reference gene.

Abstract:Objective：To investigate the effects of intracellular or extracellular gelsolin level on N-formylmethionyl-leucyl phenylala－nine（fMLP） induced rat polymorphonuclear neutrophil（PMN） chemotaxis. Methods：PMN neutrophils were isolated from rat venous blood. Freshly prepared PMN suspension was seeded to the upper chamber of Transwell culture system and recombinant gelsolin was added to the upper or lower chamber to investigate the effect of gelsolin on PMN chemotaxis. Recombinant gelsolin or anti-gelsolin antibodies were transfected into PMN with electroporation method and the chemotaxis function was observed respectively. Cell counts in lower chamber were recorded in real time. Results：There was no obvious difference in PMN chemotaxis，regardless of gelsolin was added in the upper or lower chamber or transfected into cytoplasm. However，the rate of chemotaxis and chemotaxis index of PMN electrotransfected with anti-gelsolin antibodies was decreased significantly. Conclusion：Changing intracellular or extracellular gelsolin level exerts on effect on fMLP induced PMN chemotaxis；but electrotransfection with anti-gelsolin antibodies intracellular inhibits fMLP-induced rat PMN chemotaxis.

Abstract:Objective：To explore the effect of the hypoxia-induced human retinal pigment epithelial cells（RPE） which transfected by adenovirus-mediated Slit2 and adenovirus-mediated Slit2 ShRNA on the proliferation of human choroidal microvascular endothelial cells（HCMEC），to explore the possible role of Slit2 in choroidal neovascularization（CNV） and to provide new ideas for the treat－ment of choroidal neovascularization. Methods：RPE cells and HCMEC cells were identified and cultured in vitro. Hypoxic conditions were achieved by adding 200 ?滋mol/l cobalt chloride to medium to mimic hypoxia. RPE cells and HCMEC cells were cultured in a contact co-culture system by Transwell chamber. The hypoxia RPE cells were randomly divided into Slit2 treated group（adding Slit2RNA），Slit2 shRNA treated group（adding Slit2 shRNA），empty adenovirus group（adding empty adenovirus），hypoxia group. CCK8 assay was carried out to determine the proliferation of HCMEC cells after 12，24，48 h. Results：There were statistically signifi－cant differences among different groups（F=98.122，P=0.000） and different time points（F=3388.913，P=0.000）. The interaction be－tween different groups and time points were also different（F=82.863，P=0.000）. The absorbance（A） value was higher in Slit2 treated group than in other groups（hypoxia group：P=0.001；other groups：P=0.000）. The A value was lower in Slit2 shRNA treated group than in other groups at 24 h and 48 h（at 48 h：hypoxia group，P=0.003；empty adenovirus group，P=0.008；other groups，P=0.000）. Conclusion：High-expression of Slit2 can significantly promote the proliferation of HCMEC cells. Silencing Slit2 shRNA expression in RPE cell can significantly inhibit the proliferation of HCMEC cells.

Abstract:Objective：To investigate the relation between nitrite solutions with different concentrations and methemoglobin formation rates，to invent a rapid，safe and economical detection card for blood methemoglobin concentration in human and to provide references for forensic science and clinical work. Methods：According to the dose-effect equation between nitrite solutions with different concen－trations and methemoglobin formation rate，nitrite solutions with different concentrations were obtained for generating methemoglobin blood samples with different concentrations（10%-80%）. Then，blood samples from healthy donors and methemoglobin blood samples with different concentrations were dropped on the blood collection cards. The values of red，green，blue（RGB） were acquired，and different methemoglobin color-pieces were generated. Finally，the rapid detection card was successfully fabricated. Results：Nitrite solution with different concentrations and methemoglobin formation rates was in linear correlation.With the increasing of concentration of methemoglobin in the blood sample，the color of the detection card became more and more dark. Conclusion：This detection card is rapid，safe and economical for methemoglobin concentration detection. Therefore，it might be widely applied in the future.

Abstract:Objective：To investigate the diagnosis of acute leukemia by applying FISH combined with G-banding chromosome analysis. Methods：Bone marrow specimens were collected from 410 children with acute leukemia（AL） from 2011 January to 2012 December. The cells were cultured and chromosome analysis was made by using G-banding method. Among them，57 AL cases were simultane－ously examined by FISH with their corresponding alteration genes. Results：The chromosomal abnormal rate was 41%（168/410） in 410 AL cases. Among the 57 AL cases simultaneously examined with FISH and chromosome analysis，the chromosome abnormal rate of G-banding method was 52.6%（30/57）. However，the abnormal rate of FISH was 68.4%（39/57）. The consistency of G-banding chromo－some analysis and FISH was 82.5%. Conclusion：FISH is superior to G-banding chromosome analysis when analyzing chromosome aberration in acute leukemia. Applying FISH combined with G-banding could improve the accurate and reliable results for clini－cal diagnosis of acute leukemia.

Abstract:Objective: To comprehensively evaluate the influence of thalassaemia pregnancy outcomes on pregnant woman, and to provide basis for making clinical decision. Methods: Mediterranean anemia and pregnancy outcomes of pregnant women cohort study from January 1980 to October 2014 were collected. The databases of Chinese Biomedical Literature Database, CNKI database, WanFang, VIP, Medline, ISI Web of knowledge, Elsevier, EMBase and the Cochrane library were retrieved. RevMan5.2 software was used to improve the comprehensive quantitative analysis; subgroup analysis methods were adopted to analyze the source of the heterogeneity, and the funnel chart analysis publication bias was used. Results: The Mediterranean anemia was a risk factor for pregnant women with cesarean section and premature birth(RR=1.35, 95%CI[1.19,1.54]，P=0.00; RR=2.06，95%CI[1.29,3.28]，P=0.00)， not a risk factor for neonatal low weight（RR=1.56，95%CI[0.98,2.49]，P=0.06）. Based on subgroup analysis, alpha thalassaemia was not a risk factor for pregnant women with cesarean section, premature birth and low weight（RR=1.32，95%CI[0.92,1.91]，P=0.14;RR=1.21,95%CI[0.51,2.86],P=0.67；RR=1.27,95%CI[0.52,3.10]，P=0.59）. Beta thalassaemia was not a risk factor for preterm delivery of pregnant women（RR=2.53，95%CI[0.78,8.18]，P=0.12）. Beta thalassaemia was a risk factor for low cesarean section and neonatal weight, and risk are respectively 1.54 times and 2.31 times higher than normal pregnant women（RR=1.54，95%CI[1.20,1.97]，P=0.00；RR=2.31，95%CI[1.04,5.14]，P=0.04）.Conclusion: Thalassaemia is a risk factor for pregnant with cutting palace and preterm delivery, and beta thalassaemia is a risk factor for low cesarean section and neonatal weight.

Abstract:Objective：To explore the effect of lentivirus-mediated shRNA silencing Yes-associated protein（YAP） gene on prolifera－tion，apoptosis，and androgen receptor of hormone dependent human prostate cancer LNCap cells. Methods：Three shRNA for YAP gene lentiviral interference vectors were infected into LNCaP cells，then the LNCaP cells stably knockdown YAP gene expression were screened by puromycin. Real-time quantitative PCR（real-time PCR） and Western blot were used to detect mRNA and protein ex－pression of YAP and androgen receptor（AR） after lentiviral interference vectors being infected respectively. shRNA lentiviral vector with the best silence efficiency was acted as experiment group. CCK-8（cell counting kit-8） assay and colony formation were applied to evaluate the cell proliferation in LNCaP cells. Apoptosis of LNCaP cells was examined by flow cytometry（FCM）. Results：YAP-shRNA lentiviral interference vectors were suc－cessfully infected into LNCaP cells. The infection efficiency of lentiviral interference vectors was more than 95% after puro-mycin selection and the stable YAP knockdown in LNCaP cells was established. Compared with those in control group and negative control group，expressions of YAP mRNA and protein were significantly reduced after YAP-shRNA lentiviral interference vectors being infected（P=0.00），meanwhile expressions of AR mRNA and protein were also significantly down-regulated（P=0.000）；in YAP-shRNA lentiviral interference vectors group，cell proliferation was inhibited，and apoptosis was increased significantly（P=0.000）. Conclusions：lentivirus-mediated shRNA silencing YAP gene can down-regulate the expression of YAP gene，inhibit the proliferation and promote apoptosis in LNCaP. YAP may act as a ligand of AR and regulate androgen receptor signaling pathway.

Abstract:Objective：To explore the effects of Rolipram on cognitive impairments induced by epilepticus status in the immature brain. Methods：Ninety six P21 SD male rats were randomly divided into four groups：N+Vehicle（N+Veh），N+Rolipram（N+Ro），SE+Vehicle（SE+Veh） and SE+Rolipram（SE+Ro）. Licl-Pilo was used to build the SE model. The animals received vehicle（5% DMSO） or Rolipram（0.03 mg/kg） once a day（ip） for two week. The cognitive functions were tested by Morris water maze，step-through test and hippocampus long-term potentiation；the phosphorylated cAMP response element binding protein（pCREB） levels were evaluated by Western blot. Results：Compared with those of （N+Vehicle） group，in the Morris water maze test，the（SE+vehicle） group’s escaping la－tency period was significantly longer（the third day and fourth day：P<0.01 and P<0.05）；the time in the target quadrant was longer in probe trail（P<0.01），cross-platform times were decreased（P<0.05）；in the step-through test，the（SE+vehicle） group’s escape latency was shorter（P<0.001）；the slop of fEPSP in hippocampus long-term potentiation was reduced at 30 min after HFS（P<0.05）；in Western blot test，pCREB levels were decreased（P<0.001）；and all of these were rescued with Rolipram treatment. Compared with those of （SE+Vehicle） group，in the Morris water maze test，the（SE+Rolipram） group’s escaping latency period was significantly shorter（the fourth day：P<0.05）；the time in the target quad－rant was shorter in probe trail（P<0.05），cross-platform times were increase（P<0.05）；in the step-through test，the（SE+Rolipram） group’s escape latency was longer（P<0.01）；the slop of fEPSP in hippocampus long-term potentiation was increased at 30min after HFS（P<0.01）；in Western blot，pCREB levels were in－creased（P<0.01）. Conclusion：These results indicate that the PDE4 inhibitor rolipram rescues cognitive impairments after SE，and this may be mediated through increased pCREB levels.

Abstract:Objective：To observe the ability of cell proliferation，anti-apoptosis，and angiogenesis of rat artery endothelial cells （RAECs） after survivin（SVV） gene transfection. Methods：The SVV was transfected into rat artery endothelial cells（RAECs） by adenovirus. The proliferation rates of RAECs with or without SVV transfection were determined by CCK8 assay .The levels of birc5，caspase-3，bcl-2 and cyclin D before or after transfection were detected by real-time PCR and Western blot. The apoptosis of RAECs was induced by H2O2 and the apoptosis rate in each group was detected by flow cytometry. The angiogenesis was detected by immuno－histochemistry with the RAECs specific marker CD31. Results：The birc5 gene and survivin protein levels were both elevated in SVV over-expressed RAECs determined by RT-qPCR and Western-blot assay（birc5：P=0.006，SVV protein：P=0.000）. Meanwhile，the Caspase-3 protein levels were decreased and the bcl-2，cyclinD levels were increased（Caspase-3：P=0.000；bcl-2：P=0.000；cyclinD：P=0.000）. RAECs transfected with SVV exhibited a significant higher proliferation rate. The microvessel density（MVD） was much higher in RAECs transfected with SVV than in control group. Conclusion：The ability of proliferation，anti-apoptosis and angiogen－esis is significantly elevated in RAECs after SVV transfection.

Abstract:Objective：To investigate the relationship between age and the methylation of CpG loci，which is in the neuronal pentraxin Ⅱ（NPTX2） gene’s promoter region. Methods：Genomic DNA was extracted from 170 cases of peripheral blood samples of living，and then modified by methylation modification kit. The NPTX2 gene promoter methylation status was detected by the methylation spe－cific-PCR（MSP） method，and its relationship with gender，age was analyzed. Results：Age and sex of each sample were taken as inde－pendent variables while whether having methylation was taken as dependent variable. Binary logistic regression analysis showed that statistical significance was not found between whether having methylation and gender factor（P=0.843），suggesting that gender does not affect NPTX2 gene methylation；and statistical significances were found between whether having methylation and age factor（P=0.000），suggesting that age factors significantly influences the gene methylation. Taking five-year-old as an age group，linear re－gression analysis revealed that there were linear relationship among male（P=0.001），female（P=0.000） and the overall（P=0.000），indi－cating that methylation rate is linearly increased with the increase of age. Conclusion：The methylation rate of NPTX2 gene promot－er increased with age. Further research may establish a regression model for age estimation.

Abstract:Objective：To investigate the relationship between ATG4A gene rs807185（T/A），ATG16L1 gene rs2241880（A/G） polymor－phism and the susceptibility to pulmonary tuberculosis（PTB） in the Southwestern Chinese population. Methods：The single nucleotide polymorphism（SNP） of the ATG4A gene rs807185 and ATG16L1 gene rs2241880 were genotyped by polymerase chain reaction-re－striction fragment length polymorphism（PCR-RFLP） in 218 cases versus 248 controls. Logistic regression was used to analyze the correlation between the site and the susceptibility to PTB. Results：Frequencies of A allele（OR=0.69，95%CI=0.51 to 0.92，P=0.012），AT genotype（OR=0.53，95%CI=0.30 to 0.92，P=0.025），and AA+AT vs. TT（OR=0.62，95%CI=0.42 to 0.92，P=0.017） were reduced significantly in cases than in controls. In female respondents，aforementioned indices did the same. The frequencies distribution of geno－type，allele and dominant/recessive model in rs2241880 site had no significant difference（P >0.05） in cases and controls. Conclusion：Genetic variant in ATG4A gene（rs807185） are associated with pulmonary tuberculosis susceptibility in a Southwestern Chinese pop－ulation. However，the SNP of ATG16L1 rs2241880 might not be associated with the susceptibility to PTB.

Abstract:Objective：To explore the correlation between hypoxia induced factor-1α（HIF-1α） and MicroRNA210（miR-210） in cer－vical cancer． Methods：The expression of HIF-1α protein was detected by immunohistochemistry. Poly（A）-RT-qPCR was used to de－tect the expression of miR-210 in cervical cancer and normal cervical tissues. The correlation between the expression of miR-210 and HIF-1α was analyzed in cervical cancer. Results：The expression of HIF-1α in cervical cancer was 73.1% （38/52），but HIF-1α was not detected in normal cervical tissues；the expression of miR-210 was significantly increased in cervical cancer tissues than in normal cervical tissues（P=0.000）. The expression of miR-210 in normal cervical tissues and cervical cancer was 1.16±0.76 and 5.04±1.28，respectively. The expression of HIF-1α and miR-210 were positively correlated in 52 cases of cervical cancer（r=0.43，P=0.001）. The expressions of HIF-1α and miR-210 were not correlated in normal cervical tissues. The expressions of HIF-1α and miR-210 were positively correlated in 72 cases（r=0.67，P=0.000）. Conclusion：The expression of HIF-1α and miR-210 are highly expressed in SCC tissues. HIF-1α and miR-210 are positively correlated in cervical cancer. It is predicted that HIF-1α and miR-210 are re－lated with the occurrence and development of cervical cancers，which is conductive to revealing the pathogenesis of cervical cancer.

Abstract:Objective：To investigate the effects of recombinant human lactoferrin（rhLF） on the expression of toll-like receptors 4 （TLR4） signaling pathway-associated membrane molecule（TLR4，CD14 and MD2） in lipopolysaccharide（LPS）-activated U937 macrophages. Methods：U937 was induced to maturation by phorbol 12-myristate 13-acetate（PMA） and then divided into six groups：control group，LPS group（100 ng/ml LPS），LPS+50 μg/ml rhLF group，LPS+100 μg/ml rhLFgroup，LPS+200 μg/ml rhLF group and LPS+500 μg/ml rhLF group. The mRNA and protein expressions of TLR4，CD14 and MD2 were determined by RT-PCR and Western blot. The concentration of tumor necrosis factor-α（TNF-α） was detected by ELISA. Results：The mRNA and protein expres－sion of TLR4，CD14 and MD2 increased significantly in LPS group than in control group（P<0.05）. The mRNA and protein expression of TLR4，CD14 and MD2 had no significant change in LPS+50 μg/ml rhLF group（P >0.05），but reduced significantly in LPS+100 μg/ml rhLF group，LPS+200 μg/ml rhLF group and LPS+500 μg/ml rhLF group than in LPS group（P<0.05）. The concentration of TNF-α increased significantly in the LPS group than in the control group（P<0.05）. The levels of TNF-α reduced significantly in LPS+50 μg/ml rhLF group，LPS+100 μg/ml rhLF group，LPS+200 μg/ml rhLF group and LPS+500 μg/ml rhLF group than in LPS group（P<0.05）. Conclusion：rhLF might have a potential protective effect on LPS induced sepsis through suppressing TLR4 transmembrane signal transduction pathway and reducing the secretion of TNF-α.

Abstract:Objective：To investigate the effects of interleukin-31（IL-31） or tacrolimus on expression of neurotrophin-4（NT4） in human Hacat cells. Methods：Human keratinocyte cell line（Hacat cells） was cultured and treated with different concentrations of tacrolimus and cell proliferation was determined by the MTT assay. Cultured HaCaT cells were treated with IL-31 alone or in combi－nation with tacrolimus for 24 h. Real-time PCR was used to detect the mRNA of NT4. Levels of NT4 in the culture supernant were measured with ELISA. Results：Tacrolimus with low concentration（10，100） ng/ml had mild effect on cell proliferation（P >0.05），while that with high concentration（1 000 ng/ml） inhibited cell proliferation（P=0.002）. Levels of NT4 in the culture supernant and NT4 mRNA expression were significantly increased in the presence of IL-31. Levels of NT4 in the culture supernant and NT4mRNA expression were significantly decreased with IL-31 and 100 ng/ml tacrolimus acting alone or in combination for 24 h. Conclusion：The expression of NT4 of keratinocyte is up-regulated by IL-31. Tacrolimus might achieve its anti-pruritus effect in atopic dermatitis by decreasing the NT4 expression of keratinocytes.

Abstract:Objective：To study the effects of using frozen tranexamic acid for intra-articular injection on blood loss after total knee arthroplasty（TKA）. Methods：Totally 45 New Zealand white rabbits unsexed were randomly divided into 3 groups to build the TKA operation model. After operation，a drainage tube was placed in the articular cavity，then the incision was sutured. Different solutions were injected in articular cavity in 3 groups by drainage tubes：in group A，normal ice normal saline 3 ml（0 ℃）；in group B，normal temperature tranexamic acid 3 ml（concentration of 30 mg/ml）；in group C：frozen tranexamic acid 3 ml（concentration of 30 mg/ml，0 ℃）. The drainage tube was clamped for 4 h，then opened to keep continuous vacuum aspiration. The volumes of drained blood at the 24 h and the decreasing of Hb level at the third day after operation were recorded and compared. Results：At the 24 h after operation，the volumes of drained blood in 3 groups were group A（9.61 ± 1.31） ml，group B（6.37±1.25） ml，group C（4.13±0.88） ml. The volumes of drained blood in the group C was fewer than that of in the group A（P=0.000） and group B（P=0.000）. The decreasing ranges of Hb in 3 groups were group A（17.73±4.16） g/L，group B（22.80±3.23） g/L，group C（13.80± 3.82） g/L. The decreasing range of Hb in group C was fewer than that in group A（P=0.000） and group B（P=0.006）. Conclusion：The frozen tranexamic acid can reduce the volumes of drained blood and the decrease Hb more effective than the normal temperature tranexamic acid when it is injected in the rabbit knee joint cavity after TKA.

Abstract:Objective：To examine whether Akt inhibitor treatment during the phase of oxygen-induced retinopathy helps to prevent retinal neovascularization. Methods：Seven-day-old C56BL/6J mice were exposed to（75±2）% oxygen for 5 d，then returned to the room air to induce rentinal neovascularization. Fourteen mice were equally randomized into control group and experiment group. On P12，1.5 μl Akt inhibitor was injected into vitreous cavity of mice in experiment group and PBS was injected into mice in control group. Retinal neovascularization was analyzed with FITC-Dextran and histological analysis on P17. Results：Mice treated with Akt inhibitor had a reduced pathologic neovascular tufts and fluorescence leakage compared with those of PBS controls. For the area of capillary-free，no difference was seen between experiment group and control group. The histological analysis showed that neovascular nuclei protruding into viteous cavity were decreased in experiment group compared with those in control group. Conclusion：Akt inhibitor treatment protects the retina from vessel pathological proliferation，but cannot protect the retina from vessel loss.

Abstract:Objective：To investigate the effect of preterm combined with premature rupture of membranes（PPROM） on maternal and preterm infants by observing the pregnancy outcome of pregnant women with PPROM. Method：Totally 91 pregnant women with PPROM in our department of obstetrics and gyne－cology from Jul，2013 to Jun，2014 were enrolled as study group. Meanwhile，89 women of premature delivery without rupture of membranes were selected as control. The pregnancy outcome of the two groups were compared. Results：In study group，cesarean section rate was 68.1%（62/91），and the rate of postpartum hemor－rhage was 8.8% （8/91）. In control group，rates of cesarean section and postpartum hemorrhage were 21.3%（19/89） and 1.1%（1/89）. For the outcome of newborn，rates of fetal distress，neonatal pneumonia，neonatal asphyxia and low-birth weight were 50.5%（46/91），7.7%（7/91），9.9%（9/91） and 14.3%（13/91） in study group，while were 9.0%（8/89），1.1%（1/89），2.2%（2/89） and 5.0%（4/89） in control group. The adverse event rate was significantly higher in study group than in control group，including cesarean section，post－partum hemorrhage，fetal distress，and the incidence of low body weight（P<0.05）. Conclusion：PPROM is obviously harmful for preg－nancy woman and newborn，which we need take positive strategy to prevent and handle with.

Abstract:Objective：To investigate the expression and clinical significance of CD34，COLⅠ and CXCR4 in bronchopulmonary dys－plasia（BPD）. Methods：Expressions of CD34，COLⅠ and CXCR4 in peripheral blood from 44 premature infants with BPD on the 1st，3rd，5th，7th and 28th d after birth were measured by flow cytometry（FCM） and the expression differences of CD34，COL I，CXCR4 between BPD group and the non- BPD group were compared. Results：On the 1st d after birth，the expression of CXCR4 increased greatly in BPD group compared with that of non-BPD group. With the increase of days after birth，COLⅠlevel peaked on 28th d in BPD group，while CD34 and CXCR4 levels reduced to the lowest. Data of ANOVA for repeated measurement showed that time and the changes in CD34，COL I and CXCR4 levels caused an interactive effect on the occurrence of BPD（F=77.66，P=0.000）. ROC curve displayed that area under the curve of COL I was 0.867，higher than those of CD34 and CXCR4. When COL I was 1.3%，the sensitivity was 69.7% and specificity was 86%. There were statistically significances in BPD group among different time points（P=0.000）. Conclusion：Circulating fibrocytes with increased level of COLⅠas well as decreased level of CD34 and CXCR4 are closely related with the occurrence of BPD.

Abstract:Objective：To evaluate the clinical effects of bronchial artery embolization（BAE） on refractory hemoptysis. Methods：Forty-four patients with hemoptysis were treated with BAE using one or more of polyvinyl alcohol（PAV），metallic colis and Onyx. Results：Totally 48 times of BAE were successfully performed on all pa－tients，who stopped hemoptysis with the overall effective rate of 90.9%. Hemoptysis recurred in 4 cases with the recurrent rate of 9.1%. After BAE，6 patients developed chest pain and 1 patient developed abdomen pain，but all relieved without any treatment. No case of ectopic embolization，spinal cord injury and other serious complication was observed. Conclusion：Bronchial artery embolization is a fast，safe and effective approach in the treatment of refractory hemoptysis.

Abstract:Objective：To improve the level of clinical diagnosis and treatment by studying the common symptom，treatment and prog－nosis of mushroom poisoning in children. Methods：The primary symptoms，treatment and prognosis of 49 cases of mushroom poisoning in children were retrospectively reviewed. Results：The primary symptoms of mushroom poisoning were diverse；the most common pri－mary symptom was vomiting（93.9%），followed by diarrhea（55.1%），abdominal pain（44.9%），depression（20.4%），convulsion （18.4%），dizziness（16.3%），bloody stools（14.3%），jaundice（12.2%），coma（12.2%），anuria（10.2%），headache（10.2%），delirium （8.1%），edema（6.1%），fever（6.1%），cough（4.1%），etc. There were 36 children had liver cell damage；28 children with alanine amino－transferase（ALT） higher than 1 000 U/L. On the base of symptomatic and supportive treatment，35 children were given blood purifi－cation therapy（hemofiltration，hemodialysis，hemoperfusion，plasmapheresis and combined with blood purification）. Blood purification used for mushroom poisoning in children could significantly reduce ALT. Fourteen cases（28.6%） were cured，16 cases（32.7%） were turned better，19 cases（38.8%） were died. Conclusion：The symptoms are diverse in children with mushroom poisoning，which might lead to multiple organs dysfunction and serious consequences. Children with mushroom poisoning can cause severe liver cell damage and ALT increase significantly（the majority ALT is higher than 1 000 U/L）. In addition，the ALT monitoring may be used for the assessment of prognosis. Blood purification used in the treatment of mushroom poisoning in children can significantly reduce ALT and improve the prognosis.

Abstract:Objective：To explore the effectiveness of applying FAST HUGS BID in ICU patients with mechanical ventilation. Methods：Endotracheal intubation patients with successful planned extubation were divided into control group and experimental group according to the time of entering unit. Intensive care routine was used in control group while FAST HUGS BID concept was introduced in ex－perimental group. Unplanned extubation rate and average indwelling time between the two groups were compared. Results：Unplanned extubation rate was 1.73% and the average indwelling time was （7.59±6.06） d in control group，while unplanned extubation rate was 0.93% and the average indwelling time was （5.04±4.03） d in experiment group. Conclusion：FAST HUGS BID concept is scientific and effective，reducing unplanned extubation rate，significantly shortening the average indwelling time and improving the quality of critical care.