Abstract:microRNAs（miRNAs） were a group of single strand non-coding RNAs wildly spread in animals，plants and virus. Accumu－lating evidence revealed the function of miRNA in cell differentiation，cell cross talking，development，immune defense，especially in the prospect of disease diagnosis and therapy. In this article，we reviewed the research progress of miRNA，including the structure，the relationship between tuberculosis（TB） and application prospects.

Abstract:Staphylococcus aureus（S.aureus） is a common pathogen and its surface proteins play an important role in the process of infected host. The SdrE protein，the expression of which is almost free from environmental factors（temperature，metal ions），is one of the S.aureus surface proteins and may associate with S.aureus resistance to methicillin. Recently，researchers have found that phosphorylation of SdrE directly affects the toxicity of S.aureus，and SdrE can evade the host immune response by adhesion complement regulatory factor H，as well as induce platelet aggregation independently. In addition，SdrE protein has been found to play a vital role in bone infections，joint infections and osteomyelitis. This review analyzed the biological functions of SdrE，and provided a theoretical basis for the development of new specific antibacterial agents.

Abstract:Objective：To study the effects of Streptococcus pneumoniae（S.pn） capsular polysaccharide（CPS） on acute otitis media of C57BL/6 mice. Methods：TIGR4Δcps strain was built by homologous replacement construction. Wild TIGR4（5 ?滋l/ear） or TIGR4Δcps was respectively inoculated into bilateral ear cavity by puncturing the tympanic membrane；an equal amount of sterile phosphate buffered saline（PBS） was injected into the control group. The status of mice was detected every day；inflammation of middle ear ep－ithelia that deal with wild TIGR4 or TIGR4Δcps was observed through HE staining on 1，3，5 d after inoculation. The role of CPS in cell recruitment and bacterial clearance was observed by cell counts and clonal formation unit counts，and the inflammatory cytokines in the middle ear lavage fluid（MELF） were measured to compare the level of inflammation. Results：TIGR4Δcps mutant strain was con－structed successfully. Compared with those of TIGR4 group，mice inoculated with TIGR4Δcps showed lighter damage of middle ear epithelial，lower level of TNF-α and IL-6 in MELF（1 d：P=0.076，P=0.000；3 d：P=0.002，P=0.003；5 d：P=0.000，P=0.000），fewer recruited neutrophils（1 d：P=0.004；3 d：P=0.000；5 d：P=0.000），and faster bacterial clearance（1 d：t=2.659，P=0.029；3 d：t=3.717，P=0.006）. Conclusion：In the mice model of S.pn induced acute otitis media，CPS exacerbates inflammation and tissue injury，and delays clearance of bacteria.

Abstract:Objective：To investigate the proliferation of oligodendrocyte（OL） cells induced by miR-134 in Borna disease virus（BDV） infection. Methods：The expression levels of miR-134 in OL and OL/BDV were detected by real-time PCR. BDV phosphoprotein （P24） or nucleoprotein（P40） gene expression vector was used to transfect OL cells respectively；the effects of BDV P24 and P40 pro－teins on the expression of miR-134 were detected by real-time PCR. The OL cells proliferation ability was investigated by flow cytom－etry（FCM），along with miR-134 transfection and BDV infection. Results：There were significant differences in expression of miR-134 between OL and OL/BDV cells by real-time RT-PCR（P=0.000）. The expression of miR-134 was significantly increased by BDV P24 overexpression（P=0.000），but not by BDV P40（P=0.139）. Both overexpression of miR-134（P=0.016） and BDV infection（P=0.001） down-regulated the proliferation of OL cells. Conclusion：BDV P24 protein down-regulates the proliferation of OL cells by up-regu－lating miR-134.

Abstract:Objective：To determine whether the key glycolytic enzyme enolase（Eno） bind heat shock protein DnaJ directly and to analyze the binding sites between DnaJ and Eno in Streptococcus pneumoniae（S.pneumoniae）. Methods：Recombinant Eno protein was obtained through prokaryotic expression system，and its α-enolase activity was analyzed. Kunming mice were immunized with the recombinant protein to prepare the polyclonal antibody to Eno protein and the titer of the antibody was determined by ELISA. The expression levels of Eno protein in S.pneumoniae with different serotypes were determined by Western blot. The direct interaction between DnaJ and Eno was determined by biolayer interferometrvy（BLI） technology and direct binding assay. Truncated Eno protein was expressed and purified，and the binding sites of DnaJ on Eno were determined by direct binding assay. Results：The recombinant protein Eno with high purity（about 90%） was obtained after purification of Ni affinity chromatography and exhibited α-enolase activity. The antibody titer of anti-Eno in the immunized mice serum reached to 7.68×106. Western blot showed the same band with Mr 47 000 presented in the supernatant of all the 7 serotypes of S.pneumoniae. The direct binding assay demonstrated that DnaJ bound Eno in a dose-dependent manner（r=0.920，P=0.000） and a concentration dependent binding of Eno to immobilized DnaJ was detected（KD=926 nmol/L） by BLI. Binding experiments demonstrated that DnaJ bound more truncated Eno（aa1-aa100）（2.25±0.38） than the other truncated Eno proteins（0.94±0.25），（1.08±0.09），（0.75±0.12）（P=0.000，P=0.001，P=0.000）. Conclusion：DnaJ interacts with Eno directly in S.pneumoniae and primarily via the binding sites，which are localized within 100 aa of the N-terminal domain of Eno.

Abstract:Objective：To investigate the relationship between pilA and hylEfm genes and the pathogenic mechanism of enterococcus fae－cium. Methods：A multiplex PCR was applied to detect the distribution of hylEfm and pilA. The transfer mode between strains of ente－rococcus faecium was confirmed via filter matings. S1 nuclease and I-CeuI assay was performed to detect the position of pilA and hylEfm genes. Results：Totally 137 strains of enterococcus faecium were isolated，88% clinical isolates（120/137 isolates）carrying pilA genes and 15% clinical isolates（21/137 isolates）carrying hylEfm gene. Fifty strains of inpatients and 50 strains of health controls were isolated respectively；70% isolates（35/50 isolates） carrying pilA genes and 52% isolates（26/50 isolates） carriying pilA genes were respectively observed in inpatients and health controls. Highly significant differences in isolates carrying pilA genes among clinical group，inpatients group and health controls group were observed（P<0.000 1，P=0.008 0）. Filter mating confirmed that pilA and hylEfm genes can transfer between different strains through plasmid conjugation. Southern hybridization found both pilA and hylEfm genes located at a large plasmid with size running between 145.5 kb and 291 kb. Conclusion：Positive rate of Pilus-like structures protein genes pilA of enterococcus faecium isolated from our hospital is consistently high. pilA and hylEfm genes simultaneously encode on a large plasmid containing variable antibiotic resistance genes in different isolates of nosocomial enterococcus faecium strains.

Abstract:Objective：To isolate single chain variable fragments（scFv） antibAies against medullary thyroid carcinoma（MTC） from a large human phage display library and to identify the biological activity of positive clones. Methods：Thyroid epithelial cell line（TEC cell） and MTC cell line（TT cell） were used as negative and positive antigen respectively and then five rounds of ‘adsorption elution-amplification’from the large human phage display library against MTC was conducted. Specific antibAy was tested by ELISA. E.coli HB2151 was infected to induce the expression of soluble scFv. Soluble scFv was purified by HiTrapTM Anti E-tag column. The ex－pression and relative molecular weight of the soluble scFv were detected by SDS-PAGE and Western blot. Immune activity of specific soluble scFv was detected by cell ELISA. They were divided into four groups：TT cells group，SW 480 cells group，TEC cells group and control group with PBS；A450 nm was detected by microplate reader respectively. The effects of proliferation of TT cell at different concen－trations were explored by methyl thiazolyl tetrazolium（MTT） assay. They were divided into：TT cells group，SW 480 cells group and control group with PBS，A450 nm absorbance was detected by microplate reader and inhibition rates were calculated. Results：After five panning，antibAies obtained obvious enrichment. Phage ELISA and scFv ELISA results showed that positive reactions to TT cells were 65% and 50% respectively. SDS-PAGE and Western blot showed that the relative molecular weight of the soluble scFv was about 28 kD. Cell ELISA results showed that the absorbance of TT cell group（0.41±0.12） was significantly increased compared with that of SW 480 cells group（0.20±0.03），TEC cell group（0.13±0.01），PBS control group（0.07±0.01）（P= 0.000）. MTT assay showed that when the concentration of scFv was 0.1，1，10 μmol/L，the inhibition rate between the two cell lines was statis－tically significant different（t=2.881，P=0.02；t=5.073，P=0.006；t=7.324，P=0.000）. When the concentration of scFv was 10 μmol/L，the inhibition rate of TT cells reached maximum（0.59±0.15）%. Conclusion：Human anti-MTC is successfully obtained with large phage antibAy library technique.

Abstract:Objective：To research and identify the specific sequence position of the key negative regulatory element in intron7 of SLC22A3 gene on chromosome 6q26-q27. Methods：Wild type truncated plasmids of intron7 were constructed by gene recombination to stepwise locate the specific sequence position of this negative regulatory element. Specific truncated gene fragments were amplified the by four stages using bacterial artificial chromosome（including human SLC22A3 gene） as templates，and were inserted into luci-ferase report vector to construct recombinant plasmids respectively. Recombinant plasmids and internal reference plasmids were co-transfected into HEK293T cells with ratio of 50∶1，and the luciferase activity was detected after 24 h to determine which inserted frag－ment showed regulatory effect on gene expression；transcription factor binding sites were predicted in element. Results：The luciferase activities of recombinant plasmids of pGL3-pro-SLCi7-NO6，pGL3-pro-SLCi7-NO10，pGL3-pro-SLCi7-NO6.2，pGL3-pro-SLCi7-NO6.3，pGL3-pro-SLCi7-NO10.2，pGL3-pro-SLCi7-NO6.21，pGL3-pro-SLCi7-NO10.22 were decreased compared with those of pGL3-Promoter（P<0.05）；but there was no significant difference in luciferase activity between pGL3-pro-SLCi7-NO6.21-300 bp（P >0.05），pGL3-pro-SLCi7-NO10.22-300 bp（P>0.05）and pGL3-Promoter. Conclusion：The results demonstrated that intron7 of SLC22A3 gene on chromosome 6q26-q27 contains two negative regulatory elements，which might provide theoretical basis for further studies at protein level.

Abstract:Objective：To establish liver hematogenous metastasis model in nude mice through injection of C6 glioma cells to hepatic portal vein and to investigate the feasibility and accuracy of 3.0T magnetic resonance imaging（MRI） in detection of the liver metas－tasis model. Methods：Hepatic portal veins of 36 nude mice were exposed by surgical operation. Then cultured C6 glioma cells were injected into hepatic portal veins of the 36 nude mice. Every time，12 nude mice were randomly sampled for magnetic resonance scanning on 7th，14th，21st d. After scanning，these mice were sacrificed. All livers of these mice were collected for pathological exam－ination，the ability for detection of tumor metastasis and the diameter of metastatic nodules detected by the three methods were com－pared，and immunohistochemical staining was used to assay the expression of S100beta. Results：The time metastatic nodules could be detected by MRI was latter than that by HE staining（Wald χ2=5.01，P=0.025），with no obvious difference when comparing with the time morphological changes revealed（Wald χ2=0.03，P=0.853）. And there were significant differences among the diameter of metastatic nodules detected by the three methods on 14 th d and on 21st d（F14 d=14.28，P=0.000；F21 d=1 123.23，P=0.000）. Immunohistochemical staining of S100beta confirmed metastastic nodules were C6 glioma. Metastases manifested high signal in MRI T2WI characteristics. 3.0T MRI obtained more details about metastatic nodules and also showed edema around tumors. Conclusion：The liver metastasis model can be successfully established by portal vein injection of C6 cells. A clinical 3.0T MRI scanner can be used to detect formation and transform of liver metastases in this animal model.

Abstract:Objective：To test that if Beta-Arrestin1 could bind with enhancer of Zeste homolog 2（EZH2） in acute lymphoblastic leukemia（ALL） CCRF-CEM and Raji cells. Methods：The protein levels of Beta-Arrestin1 and EZH2 in the leukemia cells were determined by Western blot. The location of Beta-Arrestin1 and EZH2 in the leukemia cells was measured by confocal microscopy. Co-immunoprecipitation（CO-IP） was examined for the binding of Beta-Arrestin1 with EZH2 in leukemia cells. Results：Western blot results showed that b/Beta-Arrestin1 and EZH2 expressed in those three leukemia cells. Confocal data showed the colocaliza－tion of Beta-Arrestin1 and EZH2 in K562，CCRF-CEM and Raji cells. CO-IP assay illustrated that Beta-Arrestin1 bind with EZH2 in three leukemia cells. Conclusion：Beta-Arrestin1 could bind to EZH2 in ALL CCRF-CEM and Raji cells.

Abstract:Objective：To investigate the protective effect of Zinc on oxidative stress in vascular endothelial cells induced by uremic serum and its possible mechanism. Methods：Serum of uremic patients and healthy controls were collected and the endothelial cells were treated with serum concentration of 15%. The pas－saged endothelial cells were divided into four groups：normal serum group（N），uremia serum group（U），uremia serum with 10 ?滋mol/L zinc sulfate（U+10），uremia serum with 30 ?滋mol/L Ｚinc sulfate（U+30）. DCFH-DA as fluorescence probe was used to detect reactive oxygen species level by fluorescence microplate reader. Total antioxidant capacity was examined by assay kit with a rapid ABTS method. The expression of NQO-1 and HO-1 mRNA was measured by real-time quantitative RT-PCR. The total protein expression of HO-1，NQO-1 and the Nrf-2 was measured by Ｗestern blot. Results：Compared with those in N group，the total antioxidant capacity of endothelial cells in U group was decreased（P＜0.001） and the ROS was significantly increased（P＜0.001）；while the Ｚinc pretreat－ment（the U+10 and U+30 two group） increased the total antioxidant capacity of cells and decreased the production of reactive oxygen species（all P＜0.001）. Both mRNA and protein expressions of HO-1 and NQO-1 were significantly increased in U and Ｚinc pretreat－ment groups（P＜0.05），more prominent in Ｚinc pretreatment group（P<0.05）. There was no significant difference between U and N groups in terms of the protein expression of Nrf-2，while the Nrf-2 nucleus expression was significantly increased in Ｚinc pretreat－ment group compared with the N and U（P<0.001）. Conclusion：Zinc can improve the total antioxidant capacity and attenuate the ox－idative stress induced by uremia serum in endothelial cells through promoting the Nrf-2 nuclear translocations and increasing the expression of HO-1，NQO-1.

Abstract:Objective：To investigate the effect of SDF-1/HGF fusion protein-mediated BMSCs on proliferation and chemotaxis of HSCs，then to rebuild liver tissue by combined transplantation. Methods：BMSCs and HSCs derived from SD rat at the age of 3-4 weeks were cultured and identified. After stably serial subcultivation，recombinated adenovirus pAD-SDF-1-（GlySer）3-HGF-IRES-GFP infected BMSCs，and the efficiency of infection was detected after infection for 1，3，7，14 d by fluorescence microscope. Meanwhile，the expressions of SDF-1 and HGF were assayed by ELISA. HSCs were planted in 24 well plates containing BMSCs cytotrophoblast. They were classified as HSCs plus cell culture fluid（group A1），HSCs plus BMSCs（group B1），HSCs plus SDF-1/HGF fusion protein-mediated BMSCs（group C1）. The proliferation index of HSCs after coculture for 1，3，7，14 d were investigated. In order to exam the immigration index of HSCs influenced by SDF-1/HGF fusion protein-mediated BMSCs，three experimental groups including HSCs plus cell culture fluid（group A2），HSCs plus BMSCs（group B2），HSCs plus SDF-1/HGF fusion protein-me－diated BMSCs（group C2） through transwell system were set. Results：After BMSCs being infected by recombinated adenovirus pAD-SDF-1-（GlySer）3-HGF-IRES-GFP for1，3，7，14 d，the efficiencies of infection were （25.28±3.72）%，82.22±3.58）%，（64.85±4.48）%，（28.35±2.73）%，respectively. And the efficiency of infection was the highest after infection for 3 d（com－pared with infection for 1，7，14 d，F=718.05，P＝0.000；F=66.80，P＝0.000；F=642.78，P＝0.000）. At the same time，after infection for 1，3，7，14 d，the expressions of SDF-1 were （1.02±0.15） μg/ml，（4.51±0.52） μg/ml，（2.13±0.19） μg/ml，（1.22±0.21） μg/ml，and the expressions of HGF were （1.38±0.32） μg/ml，（5.01±0.43） μg/ml，（3.10±0.26） μg/ml，（1.62±0.27） μg/ml. According to our data，after infection for 3 d，the expressions of SDF-1 and HGF were significantly increased compared with those after infection for 1，7，14 d（P＝0.000）. After coculture for 3，7，14 d，the proliferation of HSCs were significantly increased in group B1 and group C1（P＝0.000）compared with those of group A1. And the amplification of HSCs was obviously stronger in group C1 than in group B1（P＝0.000）. In transwell test，the immigration indexes of HSCs were prominently increased in group B2 and group C2 than in group A2. Meanwhile，the immigration index of HSCs in group C2 was remarkably higher than that in group B2. Conclusion：SDF-1/HGF fusion protein-mediated BMSCs might have an key role in promoting proliferation and chemotaxis of HSCs.

Abstract:Objective：To verify the relationship between the polymorphism of rs312691，rs623011 and thyrotoxic periodic paralysis（TPP）. Methods：Totally 112 male TPP patients were recruited and taken as experimental group，127 male patients with Graves’ dis－ease but without periodic paralysis were taken as GD group and 94 normal male individuals were taken as control（NC group）. Whole genome was extracted from each participant’s peripheral blood and the target region was amplified and sequenced. Results：The risk alley frequency of rs312691 in TPP group was 0.674，significantly higher than that in GD group and NC group. The odds ratio of geno－type CC in TPP group was 5.390 times higher than that in GD group and 2.481 times higher than that in NC group. The risk alley fre－quency of rs623011 in TPP group was also 0.674，significantly higher than that in GD group and NC group. The odds ratio of genotype AA in TPP group was 5.292 times higher than that in GD group and 3.226 times higher than that in NC group. Conclusion：The KCNJ2 related rs312691 and rs623011 are significantly related to Chinese TPP.

Abstract:Objective：To investigate antiatherosclerosis effect of atorvastatin in atherosclerosis model of SD rats，and to explore effect of nitric oxide in this course. Methods：Totally 60 male SD rats were divided into 4 groups randomly：control group，high-fat（HF） group，atorvastatin（ATV） group，and ATV+ N’-nitro-L-arginine-methyl ester hydrochloride（L-NAME） group. After being fed a high-fat diet，the rats were treated with ATV and ATV combined with L-NAME from 13 to 15 weeks，respectively. Pathologic changes of aortic arch were observed. Serum lipid level was determined. Furthermore，nitric oxide（NO），total nitric oxide synthase（T-NOS），endothelial nitric oxide synthase（eNOS） and eNOSmRNA were also determined. Results：The classical atherosclerotic lesions were fund in HF group and ATV+ L-NAME group，not in control group；serum lipid in the HF group was significantly altered（P<0.05）. Levels of serum NO，eNOS，vitality of serous T-NOS and mRNA levels of eNOS in the HF group were decreased（P<0.05）. Compared with those of HF group，atherosclerotic lesions were ameliorated in the ATV group. Serum lipid in the ATV group were significantly decreased（P<0.05）. Serum NO，eNOS，vitality of serous T-NOS and mRNA levels of eNOS in the ATV group were elevated（P<0.05）. Compared with those in ATV group，serum lipid levels in the ATV+ L-NAME group were significantly elevated（P<0.05）；serum NO，eNOS，and vitality of serous T-NOS in the ATV+L-NAME group were decreased（P<0.05）. Conclusion：Atorvastatin significantly enhances the activity of serum nitric oxide system. The blockage of nitric oxide pathway significantly bates antiatherosclerosis of atorvastatin.

Abstract:Objective：To track homing of magnetically-laden dendritic cells（DCs） into the draining lymph nodes using magnetic reso－nance（MR） imaging. Methods：Mature DCs generated from murine bone marrow cells were labeled with a laboratorially-synthesized alkylated-polyethyleneimine（PEI2k） coated superparamagnetic ironoxide（SPIO） nanoparticle. Perl’s staining，confocal laser scanning microscopy（CLSM），and fluorescence activated cell sorting（FACS） were followed to analyze labeling results and expression of mature phenotype，respectively. For in vivo MR imaging，gradient amount of DCs were subcutaneously injected into the footpads of three mice. MR scanning was performed at different time points. At 72 h after injection，mice were sacrificed to extract the popliteal lymph nodes for pathological examination. Results：A final purity of about 90% DC was achieved by this culture method. Probes located in cyto－plasm after overnight labeling，as evidenced by Perl’s staining and CLSM. Labeling process did not impact obviously on the maturation phenotypes of DCs. Both MRI and pathological studies documented the successful migration of labeled DCs into popliteal nodes. Conclusion：It is feasible to utilize MR imaging to track the in vivo migration of DCs labeled with N-alkyl-PEI2k/SPIO nanoprobe.

Abstract:Objective：To study the effect of cytotoxic chemotherapy drug epirubicin on hepatitis B virus（HBV） replication in vitro. Methods：The cell viability after epirubicin treatment was detected by MTT assay. The epirubicin was added into the culture of two stable HBV-expression cell lines（HepG2.2.15 cells and HepG2-HBV1.1 cells）. The HBV replication intermediates were extracted from the cytoplasma and supernatant，and then HBV DNA copies were detected by quantitative fluorescent PCR and Southern blot. Results：HepG2.2.15 cells were treated by 0，0.1，0.2，0.5 μmol/L epirubicin respectively，and the intracellular HBV DNA copies（×106） were 3.53±0.26，5.39±0.23，9.40±0.92，19.07±1.47；there were significant differences among these groups（F=10.984，P=0.005）. Similarly，HepG2-HBV1.1 cells were treated by 0，0.1，0.2，0.5 μmol/L epirubicin respectively，and the intracellular HBV DNA copies（×106） were 2.80±0.37，5.14 ± 0.49，14.24±1.29，26.59±1.98；there were significant differences among these groups（F=13.122，P=0.003）. The Southern blot showed that the relative volume of HBV DNA replicative intermediate in HepG2.2.15 cells were 1.00±0.01 and 1.53±0.05 in control group and drug group respectively；there were significant differences between two groups（t=19.202，P=0.002）. Similarly，the relative volume of HBV DNA replicative intermediate in HepG2-HBV1.1 cells were 1.01±0.02 and 3.12±0.20，there were significant differences between two groups（t=18.034，P=0.003）. These results suggested that epirubicin promoted intracellular replication of HBV. Meanwhile，HepG2.2.15 cells were treated by 0，0.1，0.2，0.5 μmol/L epirubicin respectively，and the HBV DNA copies（×106） in the supernatant were 2.37±0.22，3.50±0.27，6.02±0.56，17.74±0.96，there were significant differences among these groups（F=20.726，P=0.001）. Similarly，HepG2-HBV1.1 cells were treated by 0，0.1，0.2，0.5 μmol/L epirubicin respectively，and the HBV DNA copies（× 106） in the supernatant were 3.75±0.35，6.05±0.48，10.28±1.01，20.86±1.81，there were significant differences among these groups（F=14.761，P=0.002）. The Southern blot showed that the relative amount of HBV DNA replicative intermediate in the supernatant of HepG2.2.15 cells were 1.08±0.02 and 2.69±0.05 in control group and drug group respectively；there were significant differences between two groups（t=58.33，P=0.000）. Similarly，the relative amount of HBV DNA replicative intermediate in the supernatant of HepG2-HBV1.1 cells were 1.03±0.01 and 3.52±0.05 in control group and drug group respectively，there were significant differences between two groups（t=85.801，P=0.000）. The results indicated that epirubicin promoted cells secreting or releasing HBV particles. Conclusion：Epirubicin has a direct role in promoting HBV replication，which may be a new mechanism of HBV reactivation after chemotherapy.

Abstract:Objective：To explore the characteristics of cardiopulmonary function in bronchopulmonary dysplasia（BPD） complicated with pneumonia. Methods：Seventy-eight infants from Shenzhen Chil－dren’s Hospital were recruited from January 2012 to June 2013 and were divided into observation group，control group 1（BPD without pneumonia），control group 2（health infants without BPD and pneumonia）. Cardio-pulmonary function of three groups was detected. Color Doppler ultrasonography was used to detect cardiac function and ultrasonic flow meter was used to detect lung function. The diagnosis of pulmonary hypertension（PH） was made based on the velocity of tricuspid valve regurgitation（TR）≥3 m/s，left-deviated interventricular septum and right ventricular hypertrophy with chamber dilation. Results：①cardiac function：twelve cases of PH were observed in observation group while no case in control groups. Systolic pulmonary artery pressure（sPAP） was higher in observation group than in control groups（P<0.001）. Pulmonary vascular resistance（PVR）was higher in observation group than in control groups（P<0.001）. PVR of control group 1 was higher than that of control group 2（P<0.001）. The acceleration time and right ventricular ejection time（RVET） were lower in observation group than in control groups（P<0.001）. The sPAP and PVR of PH group were higher than those of non-PH group（P<0.001）. The AT and RVET of PH group were lower than those of non-PH group（P<0.001）. ②lung function：The respiratory rate（RR） was higher in observation group than in control groups（P<0.001）. The ratio of time to peak tidal expiratory time and expiratory time（Tpef/Te），the ratio of time peak expiratory volume and expiratory volume（Vpef/Ve），expira－tory flow at 75%，25% of lung volume（TEF75，TEF25），expiratory flow at 25% of lung volume to peak expiratory flow（25/PF） of observa－tion group were lower than those of control groups（P<0.05）. There was no difference between PH group and non-PH group in RR（P=0.69）. The Tpef/Te，Vpef/Ve，TEF75，TEF25，25/PF of PH group were lower than those of non-PH group（P<0.05）. Conclusion：Moni－toring cardiopulmonary function is beneficial for BPD patients complicated with pneumonia，through which patients can know cardiopul－monary injury degree，detect PH at early stage and reduce mortality to some extent.

Abstract:Objective：To evaluate the efficacy and safety of valsartan and amlodipine SPC for treating hypertensive patients with stable angina pectoris. Methods：Totally 122 patients were randomly divided into group A（60 cases） and group B（62 cases）. Group A was given valsartan and amlodipine tablets treatment while Group B was given valsartan and hydrochlorothiazide tablets treatment. Both treatments lasted for 6 weeks. The blood pressures，exercise treadmill test results，resting ECG，stable angina pectoris of two groups，before and after the treatment，were monitored and the adverse drug reactions and compliance were observed. Results：The treatment of group A showed better antihypertensive effect than that of group B（group A vs. group B，P=0.000，P=0.035），and the difference was statistically significant. There were statistical significant differences in exercise treadmill test results（P=0.001，P=0.034，P=0.000，P=0.000，P=0.029，P=0.000），resting ECG（P=0.000），coronary artery events（P=0.000）between group A and group B. There was no significant difference in adverse reactions（P=0.585） and compliance evaluation（P=0.768） of both groups. Conclusion：The treatment based on valsartan and amlodipine tablets is a better treatment in smoothly lowering blood pressure and effectively reducing the stable angina pectoris，sudden coronary events，and there is no significant difference in drug safety and compliance compared with that of the other first-line antihypertensive drugs.

Abstract:Objective：To investigate the correlation between intraoperative complications during interventional therapy in children with congenital heart diseases and anesthesia management safety and to put forward corresponding preventive measures. Methods：A retro－spective analysis was made on type，incidence，cause and treatment of adverse events after undergoing different anesthesia treatments （intravenous anesthesia，laryngeal mask combined with intravenous inhalation anesthesia and tracheal intubation） in 1 792 cases of interventional therapy. Results：Incidence of adverse event related with anesthesia was 3.63%（65/1 792）. After timely treatment，all recovered well. Incidence of adverse event related with operation was 2.40%（43/1 792），including 4 death cases died with mortality of 0.22%（4/1792）. The intravenous anesthesia group had the highest incidence of adverse events（12.6%，P=0.000）. Conclusion：Choosing appropriate anesthesia methods based on patients’ different physical condition can strengthen management of high risk fac－tors and formulate appropriate urgent treatment plan. Effective peri-operative monitoring and effective cooperation can reduce the in－cidence of serious complications in children’s congenital heart disease intervention.

Abstract:Objective：To evaluate the clinical safety and efficacy of high -intensity focused ultrasound（HIFU） in the treatment of pla－centa accreta． Methods：Fifteen patients with placenta accreta were treated with the HIFU. The blood-perfused area of placenta acc－reta was observed through sulphur hexafluoride microbubbles；the placenta accreta volume and the blood β-human chorionic go－nadotropin（HCG）as well as the time of return of normal menses were evaluated by color Doppler ultrasound. Results：After the treat－ment of HIFU，the blood-perfused area of fourteen cases was disappeared. One case still had blood perfusion，the effective rate of im－mediate treatment was 93.3% and the effective rate of one-week treatment was 100%. The bloodβ-HCG of fourteen cases were sig－nificantly reduced after treatment. In addition，nine cases resumed normal menstruation. Conclusion：HIFU as a new treatment method for placenta accreta is safe and effective．

Abstract:Objectives：To investigate the prevalence and risk factors of chronic obstructive pulmonary disease（COPD） of health exam－ination population in Chongqing，and to propose scientific suggestions for the prevention and control of COPD. Methods：The spirome－try，physical examination，chest X-ray，and cardiogram were performed on 3 000 health examination people over the age of 30 in our hospital；questionnaires about risk factors of COPD were also filled out. Some suspected COPD cases obtained definite diagnosis and treatment in the respiratory outpatient department. Results：The prevalence rate of COPD in health examination people over the age of 40 was 10.10% in Chongqing，the prevalence rate in people under the age of 40（30-39 years old ） was 2.88%，and the difference was statistically significant（ χ2=39.104，P=0.000）. Multivariate Logistic regression analysis showed that elderly age，smoking，family histo－ry，special exposure history of occupation were independent risk factors of COPD（odd ratio>1），and the difference was statistically sig－nificant（P<0.01）. Conclusion：The prevalence rate of COPD in health examination population over the age of 40 is relatively high in Chongqing and the awareness of the importance of early screening of COPD through spirometry should be strengthened. The spirome－try should be performed on people who with risk factors of COPD as early as possible. The health education and prevention of risk factors should be strengthened，which would help to improve the early diagnosis and treatment of COPD，as well as reduce the inci－dence of disability and mortality.

Abstract:Objective：To explore the effects of cluster based care intervention on depression tendency of empty-nesters in nursing center. Methods：Totally 54 cases of empty-nesters were employed to do cluster based care. GDS was used before and after the inter－vention. Results：The empty-nesters depression detection rate was 22.22% before the intervention and was 7.41% after the intervention（P=0.021）. There were significant differences in GDS scores before and after intervention（P=0.000）. High and medium sections of the GDS scores significantly decreased after the intervention（P=0.000），while low section of GDS scores had no significant difference before and after the intervention（P=0.061）. Conclusion：Depression is common among empty-nesters. Cluster based care can signif－icantly reduce its incidence and tendency，especially effective for those empty-nesters who had high and medium GDS scores.

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