Abstract:Cyclin B1（CCNB1） plays a key role in regulating the G2/M phase of the cell cycle and studies gradually have shown that CCNB1 is expressed in many malignant tumors and participates in the growth，differentiation，apoptosis and metastasis of tumor cells.CCNB1 is differentially expressed in different types of tumors，and its expression changes affect the development，treatment and prog－nosis of tumors. In this paper，the research progress of CCNB1 in tumor at home and abroad was reviewed.

Abstract:WW domain-containing oxidaseredurase（WWOX） is a tumor suppressor gene located in the fragile site of human chro－mosome 16 q23.3-24.1，involving in the regulation of biological behaviors like tumor cell proliferation，apoptosis，invasion and metas－tasis. In recent years，it has been found that WWOX gene has low-or-non-expression in various tumors，such as breast cancer，liver cancer，pancreatic cancer，nasopharyngeal carcinoma，lung cancer，gastric cancer and bladder cancer，which may be related with the covalent modification of DNA methylation that inhibits its transcription. Demethylation of DNA methyltransferase inhibitors can endogenously restore the expression of WWOX and inhibit tumors’ occurrence and development. The occurrence of tumors is co-reg－ulated by genetics and epigenetics. The methylation of tumor suppressor genes is a frequent event in the early stage of tumor devel－opment，which is expected to become a potential marker for early diagnosing the tumor and evaluating its prognosis，and a target for anti-tumor therapy. This article focused on the relationship between tumors and methylation of WWOX which acts as a tumor sup－pressor gene.

Abstract:Objective：To investigate the expression of four transmembrane protein 1（TM4SF1） in non-small cell lung cancer（NSCLC） and its relationship with clinicopathological features in NSCLC patients，to construct TM4SF1 lentiviral expression vector for stably transfecting lung cancer A549 cells，and to investigate the mechanism of interaction between TM4SF1 and JAK2-STAT3 signaling pathway in lung cancer cells. Methods：Resected cancer tissue and corresponding adjacent paracarcinoma of 61 patients with NSCLC and resected benign lung tissue of 10 patients were collected. Real-time immunofluorescence quantitative PCR（qRT-PCR） and Western blot were used to detect the expression of TM4SF1 in NSCLC tissues，paracancerous tissues and normal lung tissues. Lentiviral transfection was used to detect highly expressed TM4SF1 gene in lung cancer A549 cells. The qRT-PCR and Western blot were used to analyze the expression level of TM4SF1 gene and Sox2 gene. Transwell was used to examine the effect of high-expression TM4SF1 on A549 migration. Results：The expression of TM4SF1 mRNA and its protein in cancer tissues was significantly higher than that in adjacent tissues（P<0.05） and in normal lung tissues（P<0.05）；TM4SF1 expression was not associated with age and gender，but was associated with tumor size，degree of differentiation，lymph node metastasis，and clinical stage（P<0.05）. High-expressing TM4SF1 in lung cancer A549 cell line was successfully constructed，which was able to significantly enhance the migration ability of A549 cells and up-regulate the expression level of Sox2 in the downstream of AK2-STAT3 signaling pathway. Conclusion：TM4SF1 is highly expressed in NSCLC and overexpression of this gene can promote the migration of A549 cells and up-regulate the expression level of Sox2 in the downstream of JAK2-STAT3 signaling pathway. TM4SF1 may promote the development and distant metastasis of NSCLC through this pathway，and TM4SF1 may become a potential therapeutic target for NSCLC.

Abstract:Objective：To screen the gene that may be associated with diagnosis and prognosis of colon cancer through bioinformatics. Methods：The cRNA expression profiles data set of GSE37364 and GSE41328 were obtained from the expression omnbus（GEO） and the differentially expressed genes（DEGs） between colon adenocarcinoma tissues and normal colon tissues were screened. STRING online database，Cytoscape，GEPIA online tool and Metascape online software were used to perform the DEG-associated protein-pro－tein-interactions（PPI） network analysis，to screen the hub gene，to perform the prognosis analysis and to perform the pathway analysis，respectively. Results：A total of 18 hub genes were identified（THY1，BMP4，CD44，CXCL8，GNG4，COL1A2，IL1B，SERPINE1，IGF1，SPP1，COL1A1，HGF，POSTN，TIMP1，CXCL12，GCG，IL6 and PTGS2），among which high expression of GNG4 and TIMP1 was pos－sible to have association with poor survival and participate in the regulation of PI3K-AKT，HIF-1 and chemokine. Conclusion：Our study provides some potential target genes for the diagnosis and prognosis of colon cancer and gives a clue to molecular regulation mechanism.

Abstract:Objective：To explore the possible factor of postoperative recovery room retention in gastrointestinal cancer patients with enhanced recovery after surgery（ERAS）. Methods：Consecutive cases of gastrointestinal cancer surgery with ERAS in gastrointestinal surgery department of our hospital from March 1，2019 to September 30，2019 were retrospectively analyzed and were divided into the delayed extubation group（extubation time more than 15 minutes） and the normal extubation group（extubation time less than or equal to 15 minutes）（n=75）. The gender，age，operation time，the time of the last analgesic administration to the end of the operation，and the retention time in the recovery room in two groups were analyzed. Results：The age of patients in the delayed extubation group [（66.70±9.25） years old] was significantly higher than that in the normal extubation group[（53.42±17.12） years old]（P=0.000）. The operation time of the delayed extubation group[（172.93±75.31） min] was significantly longer than that in the normal extubation group（147.80±70.62） min]（P=0.035）. The time of the last analgesic administration to the end of the operation was significantly shorter in the delayed extubation group[（32.30±22.17） min] than that in the normal extubation group[（46.09±34.64） min]（P=0.004）. The retention time in the recovery room in the delayed extubation group[（90.99±25.95） min] was significantly longer than that in the control group[（66.97±15.77） min]（P=0.000）. Conclusion：Optimizing the surgical procedure and strengthening the man－agement of intraoperative anesthesia can reduce the postoperative recovery room retention time of gastrointestinal cancer patients with ERAS.

Abstract:Objective：To investigate the feature of c-kit and platelet-derived growth factor receptor α（PDGFRA） gene mutation in 270 patients with gastrointestinal stromal tumor（GIST），as well as its association with clinicopathological features. Methods：The gene detection result and clinicopathological data of 270 GIST patients，who underwent treatment in our hospital from June 2016 to January 2019，were collected retrospectively. Results：Among 270 patients，220 patients had c-kit mutations which were mainly oc－curred in exon 11，and then exon 9. Multimutation of A502-Y503 was the unique genotype for exon 9. Deletion mutation and point mutation were the most common mutations in c-kit exon 11，which mainly occurred in location of 557-560. Among seven patients with PDGFRA mutation，D842V mutation in exon 18 was the most common type. Among all c-kit 11 mutations，the rate of point mutation or mixed mutations occurred in the stomach was lower than that of other mutation types（P=0.022）. In patients with c-kit 11 mutations，the frequency of patients who were older than 50 years was more than other c-kit exon mutations（P=0.040）. The rate of c-kit exon 9 mutation in the small intestine was higher than other mutations（P=0.000）. The rate of mitotic count<5/50 HPF in patients with PDGFRA mutation and wild type was higher than that in c-kit mutation patients（P=0.027）. CD117 positive rate in c-kit mutation patients was significantly higher than that in PDGFRA mutation and wild type patients（P=0.000）. SMA positive rate in wild type patients was higher than that in mutation patients（P=0.002）. Conclusion：GIST patients have high frequency to expe－rience c-kit/PDGFRA；mutation locations and types are closely correlated with clinicopathological features，which also are impor－tant judgements for individual treatment.

Abstract:Objective：To investigate the correlation between lymph node metastasis and microRNA expression in gastric cancer，so as to find the potential target of gastric cancer treatment. Methods：The clinical pathological information and miRNA expression of gastric cancer patients from the public database TCGA were downloaded. Patients were divided into the lymph node metastasis positive group and the lymph node metastasis negative group in accordance with the lymph node metastasis. Independent sample t-test was used to evaluate the continuous variable data of the two groups，chi square test was used to evaluate the difference between the two groups，and factors with single factor analysis P≤0.01 were included into the logistic multiple factor regression analysis. Results：Single factor analysis indicated that the expression level of 71 miRNAs had statistically significant difference between the two groups，and tumor T stage and tumor differentiation had significant difference between the two groups. Logistic regression analysis showed that：hsa-mir-3940（OR=0.713，95%CI=0.524-0.970），hsa-mir-495（OR=1.479，95%CI=1.115-1.963），hsa-mir-580（OR=0.646，95%CI=0.441-0.946），hsa-mir-6720（OR=1.366，95%CI=1.018-1.834），hsa-mir-935（OR=0.862，95%CI=0.747-0.994），T stage（OR=2.831，95%CI=1.728-4.639）. Conclusion：The expression of hsa-mir-3940，hsa-mir-495，hsa-mir-580，hsa-mir-6720，hsa-mir-935 and T stage in the gastric cancer tissue are independent influencing factors of lymph node metastasis.

Abstract:Objective：To investigate the feasibility and diagnostic value of iodixanol-carbon nanoparticles（CNPs） suspension to double tracing lymph nodes through preoperative CT lymphography（CT-LG） combined with intraoperative staining. Methods：Human col－orectal cancer cell suspension was injected under the rectal mucosa of nude mice to establish an orthotopic transplantation model of rectal cancer lymphatic metastasis. Thirty model mice were randomly divided into the iodixanol group（n=9） and the double tracer group（n=21）. The iodixanol solution under the rectal mucosa was injected in the iodixanol group，and the CNPs suspension was injected in the double tracer group. CT-LG was performed，followed by histopathological examination. Results：In the iodixanol group，the enhanced lymph nodes were observed at 5 min and 30 min. In the double tracer group，the enhanced lymph nodes were observed at 2 h，3 h，and 5 h. The best angiographic effect was obtained at 3 h. A total of 41 enhanced lymph nodes were detected by indirect CT-LG，of which 18 were in the iodixanol group and 23 in the double tracer group，consistent with the location of the biopsy lymph nodes. The sensitivity and positive predictive value of the cau－dal mesenteric lymph nodes（CLNs） metastasis in both groups were 100% when the uneven density，the irregular shape，and the filling defect were used as the diagnostic criteria. The sen－sitivity，specificity and accuracy of lymph node metastasis in the double tracer group and the iodixanol group were 78.6% vs. 81.8%，77.8% vs. 85.7%，and 78.3% vs. 83.3%，respectively. Conclusion：Indirect CT-LG with iodixanol-CNPs suspension can not only locate the lymph nodes and evaluate the metastasis before operation，but also assist in intraoperative biopsy or dissection with black-stained lymph nodes.

Abstract:Objective：To detect the expression of eukaryotic translation initiation factor 2c（eIF2c） in colorectal adenocarcinoma and to analyze its relationship with apoptosis. Methods：A total of 69 patients with colorectal adenocarcinoma were taken as the study ob－ject. Tumor tissue was taken as the observation group and normal colonic mucosa tissue was taken as the control group. The expres－sions of eIF2c and Caspase-3 were detected by immunohistochemistry method. Expression of Bcl-2 associated X protein（BAX） was detected by Western blot. Colon cancer SW480 cell line of overexpression eIF2c was constructed and expression of BAX was detected by Western blot. Results：Differences of positive rate in two groups was statistically significant（56.5% vs. 13.0%， χ2=28.750，P=0.000）. Expression of eIF2c was significantly different in maximum diameter（68.1% vs. 31.8%， χ2=8.021，P=0.005），invasion depth（81.2% vs. 35.1%， χ2=14.849，P=0.001），necrosis（48.0% vs. 78.9%， χ2=5.366，P=0.021），lymph node metastasis（83.3% vs. 42.2%， χ2=10.765，P=0.001） and tumor thrombus（87.5% vs. 47.2%， χ2=8.134，P=0.004）. Negative correlation was found between eIF2c and Caspase-3（r=-0.69，P=0.000），and eIF2c and Bax（r=-0.66，P=0.000）. According to survival analysis，eIF2c was related with survival time（ χ2=14.00，P=0.000）. Expression of BAX was decreased in colon cancer cell line of overexpressed eIF2c. Conclusion：Higher expression of eIF2c is related with clinicopathological features and prognosis in colorectal adenocarcinoma. Abnormal expression of eIF2c is re－lated with apoptosis factors.

Abstract:Objective：To investigate the correlation between expression of microRNA（mRNA） and early recurrence of hepatocellular carcinoma（HCC）. Methods：The clinical information，follow-up results and the expression of miRNAs in TCGA were downloaded from the public database. According to the recurrence time，patients were divided into the early-stage recurrence group（≤2 years recur－rence） and the late-stage recurrence group（>2 years recurrence）. Independent sample t test was used to assess the difference of con－tinuous variable data in two groups，and chi-square test was used to evaluate the difference of categorical variable data in two groups. Factors（P<0.01） was included in logistic multivariate regression analysis. Results：T-test analysis showed that the miRNA（P≤0.01） was hsa-mir-550a-1，hsa-mir-550a-2，hsa-mir-550a-3，hsa-mir-331，hsa-mir-514a-1，hsa-mir-210，hsa-mir-155，hsa-mir-mir-3170，hsa-mir-4677，hsa-mir-4677，hsa-mir-508，hsa-mir-514a-3，hsa-mir-514a-2 and hsa-mir-624. The chi-square test showed that the indicator（P≤0.01） was tumor T stage. Logistic multivariate regression analysis showed that hsa-mir-155（OR=0.660，95%CI=0.519-0.841），hsa-mir-331（OR=1.475，95%CI=1.004-2.168），hsa-mir-514a-1（OR=0.603，95%CI=0.413-0.879），hsa-mir-550a-1（OR=1.680，95%CI=1.034-2.703） and tumor T stage（OR=1.640，95%CI=1.126-2.389） were independent influence factors of early recurrence，with statistically significant differences（P<0.05）. Conclusion：Expression levels of hsa-mir-155，hsa-mir-331，hsa-mir-514a-1 and hsa-mir-550a-1 in HCC tissues and tumor T stage are independent factors influencing early recurrence of postop－erative HCC.

Abstract:Objective：To investigate the expression of circular non-coding RNA hsa_circ_001842 as an oncogene in renal cancer tissues and renal cells of patients with renal cell carcinoma（RCC），and to investigate its effects on the proliferation，apoptosis，invasion and tumorigenicity. Methods：A total of 64 cases of renal cancer specimens and adjacent tissue specimens resected from September 2018 to October 2019 in Mianyang Central Hospital were collected. The qRT-PCR was used to detect the expression of all RCC tissues and adjacent normal tissues. Caki-1 kidney cancer cells were divided into ox-NC group（transfected with over-express－ing empty plasmid），ox-circ_001842 group（transfected with circ_001842 over-expression plasmid），sh-NC group（transfected with silent empty plasmid），and sh-circ_001842 group（trans－fected with circ_001842 silencing plasmid）. Cell clone formation experiment，flow cytometry，cell scratch test and cell invasion experiment were used to detect the effect of hsa_circ_001842 on the proliferation，apoptosis，migration and invasion of RCCs. Western blot was used to detect the expression of epithelial mesenchymal transition（EMT）-related protein and MMP-9 protein in RCCs. Caki-1 cells transfected with sh-NC and sh-circ_001842 were inoculated subcutaneously in nude mice，and tumor volume and weight were measured every 7 days. Mice in each group were executed after 4 weeks and immunohistochemical techniques and qRT-PCR were used to detect the expression of EMT-related proteins in tumor tissues. Results：The expression of hsa_circ_001842 in renal cancer tissues of renal cell carcinoma patients was significantly higher than that in normal adjacent tissues（P<0.05）. Compared with the ox-NC group and the sh-circ_001842 group，the Caki-1 renal cell’s colony formation rate，wound healing ratio，cell invasion ability and E-cadherin protein in the ox-circ_001842 group and the sh-NC group were significantly increased（P<0.05），while the apoptosis rate，N-cadherin and MMP-9 protein expression were significantly reduced（P<0.05）. Nude tumor formation experiments showed that the tumor volume and weight in the sh-circ_001842 group were smaller than those in the sh-NC group（P<0.05），and the expression level of E-cadherin protein increased significantly，while expression level of N-cadherin protein was decreased significantly（P<0.05）. Conclusion：Expression of hsa_circ_001842 in renal cancer tissues and cells is up-regulated，inhibiting the proliferation，migration，invasion and tumorigenicity by inhibiting the expression of hsa_circ_001842.

Abstract:Objective：To screen key genes in the progression of bladder cancer（BC） and explore their biological effects，providing a basis for finding potential therapeutic targets. Methods：The data of BC patients were retrieved from the GEO database. Differentially expressed genes（DEGs） of muscular invasive bladder cancer（MIBC） and non-muscle invasive bladder cancer（NMIBC） were screened by R software；the cluster Profiler software package was used for DEGs function annotation and path analysis. The STRING online database combined with Cytoscape software was used to analyze protein-protein interaction（PPI） network and screen central genes and key templates. TCGA database of GEPIA online analysis tool was used for prognostic analysis and screening key genes. The expression level of key genes was validated in Oncomine Tumor Database and imported into SPSS 22.0 for statistical analysis. The expression level of key genes in different invasive BC were verified based on the immunohistochemical results of The HUMAN PROTEIN ATLAS. Results：A total of 193 DEGs were screened，among which 116 were up-regulated and 77 were down-regulated，which were mainly involved in the biological processes of extracellular matrix tissues，cellular component composition，molecular functions，and signaling pathways such as MAPK. A total of 65 central genes were obtained after PPI analysis，and the prognostic analysis of central genes showed that expression levels of six key genes were significantly correlated with the overall survival time of BC patients，with statistically significant differences（P<0.01）. Oncomine database validation showed that five key genes of CXCL12，ANXA5，ACTA2，TAGLN and COL6A2，were significantly up-regulated in MIBC，while PPARG was significantly down-regulated in MIBC（P<0.05）. Conclusion：Changes of biological effects of extracellular matrix，MAPK pathway and six key genes（CXCL12，ANXA5，ACTA2，TAGLN，COL6A2 and PPARG） on tumor invasion may become potential therapeutic targets to inhibit the progression of BC.

Abstract:Objective：To investigate the effect of PJ34，poly（ADP-ribose） polymerase-1（PARP-1） inhibitor，on the biological behavior of human prostate cancer cell line PC-3，including cells proliferation，apoptosis，migration and invasion. Methods：PJ34 with different concentration was used to treat PC-3 cells，CCK-8 assay was used to detect cell proloferation，Western blot was used to detect the expression of PARP-1，annexin V-FITC/IP double-staining method was used to detect cell apoptosis，Transwell method was used to detect changes of cell biological behavior including migration and invasion. Results：After treatment for 72 h，48 h and 24 h，PRAP-1 levels in the PJ34 group were lower than those in the control group（t=4.647，P=0.009 7；t=4.816，P=0.008 5；t=17.28，P<0.000 1）. Apoptosis rate in the PJ34 group was （17.64±4.49）%，while in the control group was （3.83±0.74）%，with statistically signifi－cant differences（t=5.261，P=0.006）. Differences in migration had statistical significance in two groups（t=23.67，P<0.0001）. In addi－tion，cell invasion had statistically significant differences（t=29.31，P<0.000 1）. Conclusion：PJ34 can inhibit PC-3 cells' prolifera－tion，apoptosis and invasion by inhibiting expression of PRAP-1.

Abstract:Objective：To study the correlation between apparent diffusion coefficient（ADC） values obtained with diffusion weighted imaging（DWI） and Gleason scores of prostate cancer，and to evaluate the predictive value of ADC for prostate cancer risk. Methods：A retrospective study was performed to 115 patients with prostate cancer confirmed by pathology in Xijing Hospital of Air Force Military Medical University from June 2015 to January 2018. All patients underwent 3.0T MRI scan and DWI examination，with b values of 0 and 2 000 s/mm2，and they were divided into three groups according to Gleason scores，low risk group（Gleason score≤6），intermediate risk group（Gleason score=7） and high risk group（Gleason score≥8）. The ADC values of prostate cancer in each group were analyzed by one-way variance and compared in pairs between groups. Pearson correlation analysis was used to explore the correlation between ADC values and Gleason scores. Results：There were 129 regions of interest（ROI） selected from 115 patients，with 29 ROIs of low risk group，48 ROIs of intermediate risk group，and 52 ROIs of high risk group. The mean and standard deviation of the ADC values of the three groups were （1.066±0.111）×10-3 mm2/s，（0.782±0.129）×10-3 mm2/s and （0.667±0.108）×10-3 mm2/s，with statistical significance（F=108.764，P=0.000） between the three groups. Multiple comparisons showed that the differences between any two groups were statistically significant（P=0.000），and the correlation analysis between ADC value and Gleason score showed a significant negative correlation（r=-0.768，P=0.000）. Conclusion：ADC values show a negative correlation with Gleason scores of prostate can－cer，which is expected to predict Gleason scores according to the ADC values.

Abstract:Objective：To investigate the diagnostic value of serum extracellular matrix metalloprotein inductor（CD147） in ovarian cancer and its effect on prognosis and recurrence. Methods：A total of 110 ovarian cancer patients，60 ovarian benign diseases patients and 50 healthy people were collected. Levels of serum CD147 were tested by ELSIA，and were compared among three groups；Serum CD147，CA125 and HE4 were compared；correlation of serum CD147 with clinical course，histology，pathologic stage，clinical stage，recurrence rate and progression-free survival（PFS） was analyzed. Results：The level of serum CD147[（137.61±86.06） pg/mL] in the ovarian cancer group was higher than that in the benign disease group and the healthy group. The sensitivity，specificity and AUC of serum CD147（72.3%，93.8% and 0.882） were similar to those of CA125 and HE4，without significantly statistical differences（P>0.05）. Levels of serum CD147，CA125 and HE4 after six chemotherapy courses were significantly lower than those before the therapy （P>0.05），and showed significant positive correlation both before and after six chemotherapy courses（r=0.321，r=0.415，P<0.01）. Levels of serum CD147 before and after six chemotherapy courses had no statistical difference in age，tumor size，menopausal status，production time，histological type，menophania age of ovarian cancer patients（P>0.05），but had significant differences in FIGO staging，pathologic stage，infiltration，lymphatic metastasisand numbers of metastases（P<0.05）. Levels of serum CD147 before therapy and after six chemotherapy courses were significantly correlated with recurrence rate and progression-free survival（χ2=22.275，log-rank=20.400，P<0.01）. Conclusion：The serum CD147 has certain significance for diagnosing ovarian cancer and its expression level is closely related to the malignancy degree，invasion，metastasis，prognosis and recurrence of the tumor. Dynamic observation of the expression level of serum CD147 can be a reference index for diagnosing ovarian cancer，selecting surgical methods，evalu－ating therapeutic efficiency and monitoring prognosis.

Abstract:Objective：To investigate the efficacy and safety of fulvestrant for treating patients with hormone receptor（HR） positive advanced breast cancer. Methods：Clinical data of 80 patients with HR positive advanced breast cancer who were treated with fulves－trant in our hospital from January 2015 to June 2016 were collected. The clinical efficacy of fulvestrant，influencing factors and ad－verse effect were evaluated. Results：The median follow-up was 6.7 months（2.0，35.6 months），median treatment cycle was 7 cycles （2，28 cycles），median progression free survival（PFS） was 9.3 months（95%CI=4.64-13.96），objective response rate（ORR） was 2.5% and clinical benefit rate（CBR） was 40.0%. COX multivariate analysis indicated that PFS was correlated with previous lines of chem-otherapy[HR=2.18（1.55，3.08），P=0.000]. Conclusion：Fulvestrant of 500 mg has a favorable efficacy in patients with HR positive advanced breast cancer，with good safety.

Abstract:Objective：To detect the expression of microRNA-496（miR-496） in ovarian serous carcinoma，so as to observe its expres－sion significance，and to analyze its correlation with homologous heteroprotein sine oculis homeobox homolog 1（SIX1）. Methods：A total of 75 patients with ovarian serous carcinoma were selected as the observation group，and another 75 patients with ovarian serous cystic adenoma were selected as the control group. Expression of miR-496 in two groups was detected by real-time fluorescence quantitative PCR. Expression of proliferating cell nuclear antigen（PCNA） and Bcl-2 associated X protein（BAX） in the observation group was detected by immunohistochemical method. Expression of SIX1 in the observation group was detected by Western blot. Ovarian serous cystic adenoma cell lines were selected；blank control group，micR-496 transfection group，and miR-496 and SIX1 co-transfection group were set up. Relationship between miR-496 and target gene SIX1 was verified by applying double luciferase gene experiment. Results：Expression of miR-496 in the observation group was significantly lower than that in the control group（1.52±0.36 vs. 2.03±0.25，t=7.56，P=0.001）. The expression of miR-496 was statistically significant in different groups of tumor maximum diameter（1.65±0.36 vs. 1.42±0.33，t=5.32，P=0.012），histological grade（1.64±0.35 vs. 1.43±0.40，t=5.11，P=0.010），vascular in－volvement（1.60±0.44 vs. 1.35±0.43，t=5.11，P=0.011），bilat－eral occurrence（1.61±0.36 vs. 1.40±0.32，t=5.11，P=0.010），lymph node metastasis（1.62±0.42 vs. 1.35±0.41，t=5.66，P=0.008） and different TNM stages（1.70±0.37 vs. 1.42±0.39，t=5.65，P=0.009）. Expression of miR-496 was related with survival time（ χ2=4.13，P=0.010）；it had negative correlation with PCNA（r=-0.54，P=0.019） and SIX1（r=-0.58，P=0.013）；it had positive correlation with BAX（r=0.52，P=0.011）. Double luciferase gene experiment results showed that miR-496 was able to significantly reduce the luciferase activity in pGL3-SIX1-WT transfected cell lines. Conclusion：Expression of miR-496 in ovarian serous carcinoma is decreased，which is the molecular factor for accelerating tumors’ formation and development. Detection of miR-496 has a certain significance for judging the prognosis. MiR-496 may regu－late cell proliferation and apoptosis by negatively regulating the expression of SIX1.

Abstract:Breast cancer is one of the most common malignant tumors in Chinese women，and is a hormone dependent tumor. In recent years，endocrine hormone pathway drugs for breast cancer have been developed and applied，and some of them have been demonstrated that can improve the prognosis，achieving good clinical results in breast cancer patients. The treatment plan for breast cancer patients is changing rapidly and endocrine hormone therapy has become the first-line treatment for the hormone receptor positive patients in breast cancer. This study has reviewed the mechanism，side effects，drug resistance and ongoing clinical research of endocrine hormone therapy in breast cancer patients.

Abstract:Glioma is the most common primary malignant tumor in the central nervous system，with poor prognosis and high morbidity and mortality. It has been confirmed that the maximum excision of tumors within a safe range is beneficial to extend progression-free survival and overall survival，and improve patients’ prognosis. Fluorescence guided technique（FGT） can realize the visualization of the edge of brain glioma during operation by using fluorescent imaging reagent，so as to assist the surgeon in more accurate tumor re－section within the safe range. At present，the fluorescence imaging reagents used in clinic include sodium fluorescein（SF），5-aminolevulinic acid（5-ALA），indocyanine green（ICG），hypericin，talaporfin sodium（TPS），etc. In addition，a large number of new fluorescent agents are actively used in clinical or preclinical trials. This paper reviewed various intraoperative fluorescence imaging reagents for gliomas.

Abstract:Objective：To evaluate the short-term clinical efficacy of transcatheter arterial chemoembolization（TACE） combined with high intensity focused ultrasound（HIFU） in the treatment of massive hepatocellular carcinoma（MHCC） complicated with portal vein tumor thrombosis（PVTT）. Methods：A total of 81 patients with MHCC complicated with PVTT in the Second Affiliated Hospital of Chongqing Medical University were enrolled in this retrospective study. According to the treatment methods，they were divided into the TACE+HIFU group（41 patients，received TACE combined with HIFU） and the TACE group（40 patients，only received TACE）. The effective treatment rate was evaluated by comparing the imaging data of tumor lesions and PVTT in two groups，and the survival quality Karnofsky score，postoperative adverse reactions and median survival time were analyzed. Pearson correlation was used to analyze the relationship between time interval of HIFU treatment after TACE and Karnofsky score one month after op－eration. Results：The tumor lesion and PVTT effective rate in the TACE+HIFU group（58.5%；48.8%） was significantly higher than that in the TACE group（20.0%；17.5%），and the difference between the two groups was statistically significant（P=0.000，0.003）. The median survival time in the TACE+HIFU group was 8 months，which was longer than that in the TACE group（4 months）（P=0.028）. The Karnofsky score one month after operation in the TACE+HIFU group was higher than that before operation；the quality of life was improved，which was better than that in the TACE group（P=0.009）. There were no serious complications in both groups during the postoperative and follow-up period. The time interval of HIFU treatment after TACE showed a low negative correlation with Karnofsky score one month after operation（r=-0.381，P=0.014）. Conclusion：For patients with MHCC and PVTT，the pattern of TACE+HIFU is a safe and effective combination therapy，enhancing the short-term curative effect. It not only can prolong survival time，but also control the progression of the tumor lesion and PVTT at the same time.

Abstract:Objective：To investigate the feasibility of using patient controlled analgesia（PCA） combined with intrathecal drug delivery system（IDDS） in patients with bone metastasis cancer pain. Methods：From November 2017 to November 2018，patients with bone metastasis cancer pain in our department were included. Thirty patients were selected in accordance with inclusive criteria and ran－domly divided into two groups according to random digits table. Observation group：IDDS was implanted and PCA was externally con－nected；morphine was continuously injected，with speed（mL/h）= daily initial dose（mg）/（concentration*24），not lower than 0.1 mL/h. Control group：oral morphine sustained release tablets were mainly used and morphine injection was subcutaneously injected to con－trol breakthrough pain. The numerical rating scale（NRS） score，the daily morphine dose and the salivary α-amylase（SAA） activity were recorded before treatment（T1），three days after treatment（T2），and one week after treatment（T3） were recorded，and adverse reaction of two kinds of analgesia were observed. Results：According to repeated measurement，NRS and SAA activity were gradually decreased with the progress of treatment，and daily morphine dosage in the observation group was gradually decreased，with significant difference between the two adjacent time points（P<0.05），and with significant difference between different treatment methods（P<0.05）. Usage of intrathecal sustained medication had fewer adverse reactions（P<0.01）. Conclusion：Compared with oral morphine，IDDS combined with PCA can make bone metastasis cancer pain alleviate more rapidly to a greater extent，which can significantly improve patients’ anxiety and stress. In addition，the dosage of morphine is small and the adverse reaction is less.

Abstract:Objective：To develop the risk prediction model of psychological distress in lung cancer patients by using structural equa－tion model. Methods：A total of 441 hospitalized patients with lung cancer were randomly selected and questionnaires or scales were used to investigate the general information，psychological distress，stigma，illness perception，social support，perceived stress，coping style，mindfulness and self-esteem. Structural equation model was used to analyze the association between those factors and psycho－logical distress. Results：The structural equation model for psychological distress in lung cancer patients had a good fitting degree and its results showed that social support had a direct negative effect（β=-0.540，P=0.001） and an indirect negative effect（β=-0.003） on psychological distress，perceived stress had a direct negative effect（β=0.181，P=0.001） and an indirect negative effect（β=0.004） on psychological distress，coping style had a direct positive effect（β=0.114，P=0.005），mindfulness（β=-0.021） and stigma（β=-0.201） had indirect negative effect on psychological distress，educational level had a direct（β=0.147，P=0.003） and an indirect positive effect（β=0.026） on psychological distress，family income had a direct negative effect（β=-0.105，P=0.027） and an indirect positive effect（β=0.108） on psychological distress，age had a direct negative effect（β=-0.101，P=0.019） on psychological distress，occupational status had a positive effect（β=0.094，P=0.032） on psychological distress，and drinking history（β=0.031） and cancer stage（β=0.031） had an indirect effect on psychological distress. Conclusion：The risk prediction model which was con－structed in the present study is acceptable for patients. Path analysis of all risk factors suggests that the psychological dis－tress of lung cancer patients is influenced by many different factors in different ways and he social support，coping style，mindfulness，stigma，age，family income，educational level，occupational status，drinking history and cancer stage are key predictors

Abstract:Objective：To analyze the domestic and foreign literatures on psychological problems and interventions of tumor patients in recent 10 years，so as to provide a basis for further improving the research level in this field. Methods：Based on the core database of Web of Science，VOSviewer software was used for the bibliometrics analysis of research on psychological problems and interventions of cancer patients. Results：A total of 2 644 articles were included，and the number of publications were increased year by year. The top one country was the United States，and China ranked fourth. The main direction of research fields involved：①psychological symptoms；②psychological studies related to quality of life；③studies related to caregivers；④studies on parents of adolescent cancer patients；⑤research on psychological assessment tools；⑥psychological research related to female cancer patients；⑦psychological research related to male cancer patients. The main intervention modes were mindfulness-based stress reduction therapy，music therapy，exercise therapy，supporting treatment，dignity therapy，complementary and alternative therapy and network intervention，etc. Conclusion：The academic research on psychological problems and interventions of cancer patients has increased. However，domestic research in this field needs to be further developed and improved. In the future，the psychological problems，resilience and decompression intervention of children，young and middle-aged patients，and terminally-ill cancer patients should be taken as the focus of research.

Abstract:Objective：To explore effectiveness of IIFAR（initial check，information exchange，final accuracy check，reaction） information care model on distress，anxiety and depression in lung cancer patients. Methods：Randomly 132 inpatients with lung cancer were enrolled and assigned to two groups. Patients in the intervention group received IIFAR information care model in addition to routine health education. And the control group received routine health education. The scores of distress thermometer（DT），hospital anxiety and depression Scale（HADS） and the causes of distress were compared between the two groups. Results：There was no significant difference between two groups before the intervention（P>0.05）. The results of inter-group analysis showed that the scores of DT，anxiety subscale and depression subscale in intervention group（1.81±1.16，1.26±1.41，2.74±2.53） was lower than those in control group（2.70±1.57，3.12±2.16，5.72±4.08） after intervention（t=3.521，P=0.001；t=5.519，P=0.000；t=-4.779，P=0.000）. The results of intra-group analysis showed that after intervention the scores of DT，anxiety subscale and depression subscale was lower than before in intervention group（3.33±1.67，3.82±2.19，6.21±4.01）（t=9.397，P=0.000；t=10.131，P=0.000；t=9.148，P=0.000），and no significant difference in control group（P>0.05）. Compared with control group，worry，sleeping，fear，tension and financial problems were alleviated in intervention group（P<0.05）. Conclusion：Information care can relieve distress，anxiety and depression in lung cancer patients.