Abstract:Objective：To investigate the effects of Atractylodes macrocephala decoction pieces on enteric neurotransmitter and stem cell factor（SCF）/stem cell growth factor receptor（c-kit） signaling pathway of interstitial cells of Cajal（ICC） in mice with slow transit constipation（STC）. Methods：The STC mouse model was replicated by subcutaneous injection of 2.5 mg/（kg·d） morphine hydrochloride injection. A total of 48 Kunming mice were randomly divided into normal group，model group，Atractylodes macrocephala decoction pieces low，medium，high-dose groups（25，50，100 mg/kg） and mosapride group（1.5 mg/kg），each with 8 mice. After each group was treated with different drugs for 1 week，the stool characteristics and intestinal propulsion rate of mice in each group were tested after modeling and administration. Enzyme-linked immunosorbent assay（ELISA） was conducted to determine neurotransmitter substance P（SP），acetylcholine（Ach），vasoactive intestinal peptide（VIP），nitric oxide（NO） and 5-hydroxytrytamine（5-HT） contents in the colon of the mice in each group. And Western blot was used to detect the expression of SCF and c-kit protein in mouse colon tissue. Results：ELISA results showed that compared with the normal group，the SP and Ach contents of the model group were significantly reduced（SP：230.41±16.41 vs. 146.69±15.59；Ach：577.68±39.35 vs. 281.50±39.40）. Compared with the model group，the SP content in the low-dose group of Atractylodes macrocephala，the middle-dose group and the high-dose group of Atractylodes macro－cephala fragments were significantly increased（146.69±15.59 vs. 188.25±4.60；146.69±15.59 vs. 201.78±4.44；146.69±15.59 vs. 307.51±16.94）；Ach content in high-dose Atractylodes macrocephala and mosapride group were significantly increased（281.50±39.40 vs. 400.93±12.21；281.50±39.40 vs. 422.22±22.70 ）. Compared with the high-dose group of Atractylodes macrocephala decoction pieces，the SP and Ach contents of the low-dose group of Atractylodes macrocephalus decoction pieces and the middle-dose group of Atractylodes macrocephalus decoction pieces were significantly reduced（SP：307.51±16.94 vs. 188.25±4.60；307.51±16.94 vs. 201.78±4.44；Ach：400.93±12.21 vs. 283.79±11.53；400.93±12.21 vs. 327.81±14.77）；SP content in the mosapride group was significantly reduced（307.51±16.94 vs. 164.08±12.54）. Compared with the normal group，the contents of VIP，NO and 5-HT in the model group were significantly increased（VIP：64.47±2.69 vs. 87.74±2.93；NO：38.21±1.76 vs. 42.78±1.69；5-HT：219.58±11.60 vs. 276.08±7.97）. Compared with the model group，the VIP content of the low-dose Atractylodes macrocephala decoction pieces，the medium-dose Atractylodes macrocephalus decoction pieces，the high-dose Atractylodes macrocephalus decoction pieces，and the mosapride group were significantly reduced（87.74±2.93 vs. 75.58±2.08；87.74±2.93 vs. 69.34±2.23；87.74±2.93 vs. 66.37±1.93；87.74±2.93 vs. 65.31±3.32）；Atractylodes macrocephala medium dose group，Atractylodes macrocephala fragment high dose group and mosapride group NO and The content of 5-HT was significantly reduced（NO：42.78±1.69 vs. 39.27±1.90；42.78±1.69 vs. 37.43±1.30；42.78±1.69 vs. 35.65±2.01；5-HT：276.08±7.97 vs. 257.89±6.16；276.08±7.97 vs. 226.79±10.49；276.08±7.97 vs. 242.05±12.15）. Compared with the high-dose Atractylodes macrocephala decoction pieces group，the contents of VIP，NO and 5-HT in the low-dose Atractylodes macrocephalus decoction pieces group were significantly increased（VIP：66.37±1.93 vs. 75.58±2.08；NO：37.43±1.30 vs. 42.73±2.19；5-HT：226.79±10.49 vs. 269.87±10.91）；VIP，5-HT in the middle-dose group of Atractylodes macrocephala and 5-HT in the mosapride group were significantly increased. Western blot results showed that compared with the model group，the c-kit protein content in the low-dose group of Atractylodes macrocephala was significantly increased（0.22±0.10 vs. 0.37±0.08）；the middle-dose group of Atractylodes macrocephala and the high-dose group of Atractylodes macrocephala both the SCF and c-kit protein content in the mosapride group and the mosapride group were significantly increased（SCF：0.60±0.19 vs. 0.99±0.28；0.60±0.19 vs. 1.17±0.34；0.60±0.19 vs. 1.02±0.30；c-kit：0.22±0.10 vs. 0.47±0.10；0.22±0.10 vs. 0.58±0.13；0.22±0.10 vs. 0.49±0.13）. Conclusion：Atractylodes macrocephala decoction pieces can increase the contents of SP，Ach and SCF/c-kit in the colon tissue of STC model mice，and reduce the contents of VIP，NO and 5-HT，thereby regulating the gastrointestinal function of mice，promoting the intestinal tract peristalsis，improving constipation symptoms，and then achieving the effect of treating STC.

Abstract:Objective：To investigate the value of conventional MRI signs combined with minimum apparent diffusion coefficient value（ADCmin） in the diagnosis and differentiation of primary central nervous system lymphoma（PCNSL） under logistic regression model. Methods：The conventional MRI characteristics and ADCmin of 34 cases of PCNSL confirmed by clinical and pathology were observed and analyzed. Another 36 cases of high-grade glioma（HGG） were selected as the control group. The differences in MRI characteristics and ADCmin between the two groups were statistically analyzed and compared，and logistic regression analysis was performed. The receiver operating characteristics（ROC） curve was used to analyze and compare the efficacy of single diagnosis and combined diagnosis for the two groups of tumors. Results：The proportions of cystic degeneration，necrosis and hemorrhage in PCNSL group were significantly lower than those in HGG group（?字2=25.2，P<0.001）. The proportion of PCNSL group in clear boundary，involving midline structure or（and） ventricle，uniform enhancement，existence of “notch sign” and（or） “sharp angle sign” and high signal signs on diffusion-weighted imaging（DWI） was higher than that of HGG group（all P<0.05）. There was no significant difference between the two groups in the degree of peritumoral edema and the degree of mass occupation（P >0.05）. The ADCmin of PCNSL group was significantly lower than that of HGG group（t=-7.962，P<0.001），while there was no statistical significance in the ADC value of the control side between the two groups（t=1.208，P=0.231）. When ADCmin was diagnosed separately，the optimal diagnostic cut-off point for the two groups was ADCmin=0.727×10-3 mm2/s，and the sensitivity and specificity of the two groups for differential diagnosis were 97.1% and 80.6%，respectively. The area under the curve was the largest（AUC=0.922） and the diagnostic efficiency was the highest. Logistic regression equation model was：Logistic（P）=15.269+3.963×uniform enhancement -24.695×ADCmin. Both ADCmin and homogeneous enhancement were influential factors for the diagnosis of PCNSL. When the optimal threshold of combined diagnosis was 0.46，the AUC of PCNSL was 0.977. The sensitivity and specificity of the two groups for differential diagnosis were 97.1% and 94.4%（P<0.001），with the highest efficacy of combined diagnosis. Conclusion：Routine MRI feature analysis combined with ADCmin can provide morphological and molecular imaging basis for non-invasive differentiation of PCNSL and HGG. Especially for the two tumors with overlapping manifestations of conventional MRI，the combined diagnosis under Logistic regression model can effectively improve the diagnostic efficiency，thus providing early diagnosis for patients with different tumors in the two groups，so as to provide reliable basis for early intervention，timely adjustment of treatment plan and judgment of prognosis value.

Abstract:Objective：To analyze the clinical characteristics of neuropsychiatric systemic lupus erythematosus（NPSLE） and explore the hazardous factor of this disease. Methods：A total of 20 NPSLE patients hospitalized in The Second Affiliated Hospital of Chongqing Medical University from January 2016 to December 2020 were taken as the research group. The neuropsychiatric symptoms，cerebrospinal fluid，brain MRI，treatment protocols and prognosis of 20 NPSLE patients were statistically analyzed. A total of 20 patients with systemic lupus erythematosus（SLE） during the same period were taken as the control group. Two sets of laboratory indicators as well as laboratory indicators before and after treatment of the research group were compared. Logistic regression analysis was performed on the related risk factors of NPSLE. Results：Compared with the control group，the anti-C1q antibody and the anti-ds-DNA antibody of the research group increased（P=0.001，0.005）；C3，C4 and serum albumin/globumin（A/G） reduced（P=0.000，0.016，0.001）. Compared with anti-C1q antibody，anti-ds-DNA antibody，immune globulin IgA and immune globulin IgG of the research group before treatment and these laboratory indicators after treatment reduced（P=0.000，0.001，0.006，0.000）；C3，C4 and A/G increased（P=0.000，0.000，0.000）. The univariate logistics regression analysis indicated that A/G，anti-ds-DNA antibody，C3 and C4 were related to NPSLE. The multivariate logistics regression analysis indicated that C3 was related to NPSLE（OR=0.000，95%CI=0.000-0.417，P=0.034）. Conclusion：Compared with SLE，C1q antibody and anti-ds-DNA antibody of NPSLE patients are increased，and their titer decreases after treatment；C3，C4 and A/G are decreased，and their indexes increase after treatment. The indexes of immunoglobulin IgA and immunoglobulin IgG in NPSLE patients decrease after treatment. Anti-C1q antibody，anti-ds-DNA antibody，C3，C4，A/G，immune globulin IgA and immune globulin IgG are closely correlated with NPSLE，and they are key indicators reflecting the activity of NPSLE. Decline in C3 is an independent risk factor for NPSLE.

Abstract:Objective：To explore the effect of exercise-walking dual-task training on walking function in patients with stroke. Methods：A total of 56 hemiplegic patients with stroke were randomized into control group（n=27） and experimental group（n=29）. The control group was given routine walking training，and the experimental group was given exercise-walking dual-task training as the same time and frequency as the control group. The integrated electromyography（IEMG） of maximal isometric contraction of rectus femoris，biceps femoris，tibialis anterior muscle and gastrocnemius muscle，and the co-contraction rate（CR） of knee flexion，knee extension，ankle dorsiflexion and ankle plantarflexion were recorded before intervention and after 4 weeks of intervention. The single-task walking speed，dual-task walking speed and dual-task walking speed cost（DTC） during 10 m walking test were calculated. Results：A total of 26 cases in the control group and 27 cases in the experimental group completed the study. After 4 weeks of intervention，the IEMG，CR and walking speed of patients in both groups were significantly improved as compared with those before intervention（P<0.01）. During knee extension and ankle dorsiflexion，the IEMG of rectus femoris and tibialis anterior muscle in the experimental group was higher than that in the control group（P<0.05），while CR was lower than that in the control group（P<0.05）. The walking speed of the experimental group under dual-task was higher than that of the control group（P<0.001），and the DTC was lower than that of the control group（P<0.001）. Conclusion：Exercise-walking dual-task training can improve the walking function of patients，and compared with routine walking training，it’s more helpful to the coordination between agonistic muscle and antagonistic muscle of hemiplegic lower limbs.

Abstract:Objective：To analyze the related risk factors of residual headache in patients with cerebral venous sinus thrombosis（CVST）. Methods：The study retrospectively analyzed the clinical data of CVST patients who met the inclusion criteria and had complete medical records admitted to The Second Hospital of Bazhou from January 2016 to June 2019，and analyzed the related risk factors of residual headache in CVST patients using univariate and multivariate logistic regression methods. Results：A total of 111 CVST patients met the inclusion criteria，of which 38（34.23%） patients had residual headache. The univariate analysis showed that there were significant differences in the past history of headache（Z=-3.723，P=0.000），delayed anticoagulation（Z=-3.519，P=0.000），and the failure of venous sinus recanalization（Z=-3.071，P=0.002） between the residual headache group and the non-residual headache group. Multivariate regression analysis found that the failure of venous sinus recanalization was a risk factor for residual headache（OR=6.419，95%CI=1.922-21.438，P=0.003）. Secondly，delayed anticoagulation（delayed for more than 7 days） increased the risk of residual headache（OR=5.248，95%CI=1.710-16.105，P=0.004），and past headache history was also an independent risk factor for residual headache（OR=11.98，95%CI=2.451-53.931，P=0.002）. Conclusion：Venous sinus recanalization，delayed anticoagulation and past headache history are independent risk factors of residual headache in CVST patients. Timely anticoagulation and recanalization of cerebral veins in patients with CVST may be of great significance to reduce the occurrence of residual headache.

Abstract:As a potential target that affects the proliferation of neural stem cells，non-coding RNA（ncRNA） has become a key influ－encing factor to improve the damage of central nervous system in recent years. Although neural stem cells are not easy to proliferate in adult neural tissues，ncRNA has the effect of regulating cell renewal，and the interaction between microRNA（miRNA） and long non-coding RNA（lncRNA） is complex and diverse. Therefore，in recent years，the two have become the hot spots of the study of regulating the proliferation of neural stem cells. This article summarizes how miRNA，lncRNA，and the combined effects of miRNA and lncRNA affect the proliferation of neural stem cells in recent years，and conducts an in-depth analysis of the limitations of current research，focusing on the studies of physical agents mediated neural stem cell proliferation in the field of rehabilitation medicine，providing a direction for future research in related fields.

Abstract:Trace amine receptor 1（TAAR1） is a new member of the G protein-coupled receptor（GPCR） family which was discov－ered in 2001. Related studies have found that trace amine（TA） is highly sensitive and can mediate TA regulation of the activity of dopamine（DA） neurons. TAAR1 itself is also a regulator of the DA system，which is closely related to the normal function of the DA system. The disorder of DA energy system can also lead to Gilles de la Tourette syndrome（TS）. Therefore，TAAR1 provides a new target and direction for exploring the pathogenesis of TS.

Abstract:Remyelination disorder after spinal cord injury（SCI） is the main cause of neurological dysfunction in patients. As the glial cells of the peripheral nervous system（PNS），Schwann cells（SC） are involved in the formation and repair of myelin sheath. However，more and more studies have shown that SC can be observed at the injured site after SCI. In this paper，we have reviewed the cell source，repair mechanism and regulatory factors of SC in the process of SCI repair.

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Abstract:Objective：To compare the therapeutic effects of transthoracic minimally invasive closure and transcatheter closure of atrial septal defect（ASD） guided by echocardiography. Methods：In the study，80 patients treated with transthoracic minimally invasive clo－sure of atrial septal defect were selected as the study group，and 112 with transcatheter closure of atrial septal defect were selected as control group. Statistical methods were used for the comparison between the two groups. Results：In the study group，79 cases were successfully occluded and 1 case failed，with a success rate of 98.8%；in the control group，109 cases were successfully occluded and 3 cases failed，with a success rate of 97.3%. In the study group，there were 3 cases of residual pericardial effusion after operation，and the rest had no other complications；1 cases of femoral vein thrombosis，1 cases of puncture hematoma and 5 cases of pericardial effusion were found in the control group after operation. All patients were followed up for one year. The transverse diameter of right atrium，right ventricle and pulmonary artery in the study group and the control group were both significantly smaller than those before operation，and the area of tricuspid regurgitation was significantly reduced（P<0.01）. The type of occluder used in the study group was positively correlated with the maximum diameter of ASD screened by echocardiography（r=0.940，P<0.01）. The type of occluder used in the control group was also positively correlated with the maximum diameter of ASD screened by echocardiography（r=0.928，P<0.01），and the correlation in the study group was slightly higher than that in the control group. Conclusion：Transthoracic minimally invasive closure of atrial septal defect has a high success rate and few complications. The therapeutic effect is better than that of transcatheter closure，and it is of important clinical value.

Abstract:Objective：To investigate the effect of coated pitavastatin nanoparticles on re-endothelialization of injured blood vessels and its mechanism of action. Methods：Endothelial progenitor cells were cultured with pitavastatin nanoparticles and transplanted into a rat carotid artery injury model. CCK-8 method was used to observe the proliferation of smooth muscle cells in the injured vascular media. One week later，Evan’s blue staining was used to observe the re-endothelialization，and hematoxylin-eosin staining was used to observe the neointimal hyperplasia of the injured blood vessels. Results：Three days after transplantation of endothelial progenitor cells that endocytosed pitavastatin nanoparticles，fluorescence microscopy showed that endothelial progenitor cells accumulated at the injury site，and the number of homing endothelial progenitor cells in the pitavastatin nanoparticle group was higher than that in the pitavastatin drug group（P=0.025）. Compared with the pitavastatin drug group，the pitavastatin nanoparticle group could significantly inhibit the proliferation of injured vascular smooth muscle cells（P=0.013）. Endothelial progenitor cell transplantation could inhibit intimal proliferation and promote the re-endothelialization of injured blood vessels. The effect of pitavastatin nanoparticles group was more significant than that of the pitavastatin drug group（P=0.043，0.024，respectively）. Conclusion：Pitavastatin nanoparticles can be taken up by endothelial progenitor cells，which can promote their homing to damaged blood vessels，and inhibit smooth muscle cell proliferation at the same time，thereby effectively inhibiting intimal proliferation and promoting re-endothelialization of damaged blood vessels.

Abstract:Objective：To investigate the effects of different doses of rosuvastatin on ventricular remodeling in elderly patients with hypertension and chronic heart failure with preserved ejection fraction（HFpEF）. Methods：A total of 128 elderly patients with hyper－tension and HFpEF were randomized into two groups，the low-dose group（64 cases，10 mg/d） and the high-dose group（64 cases，20 mg/d），based on conventional treatment for heart failure，both groups were given rosuvastatin at night，and the treatment period of both groups was 6 months. Before and 6 months after treatment，we monitored changes in the levels of left ventricular remodeling related indexes in patients including left ventricular end-diastolic posterior wall thickness（LVPWT），interventricular septum thickness （IVST），and left ventricular mass index（LVMI），left ventricular diastolic function related indicators including isovolumetric relaxation time（IVRT），deceleration time（DT），left atrial volume index（LAVI），and E/E'，cardiac function related indicators including Tei index，N-terminal-pro-B-type natriuretic peptide（NT-proBNP），and 6 minute walking test（6-MWT），myocardial fibrosis related indicators including galectin-3（Gal-3），soluble ST2（sST2），connective tissue growth factor（CTGF），and transforming growth factor-β1（TGF-β1） and inflammatory response indicators including hyper-sensitive C-creative protein（hs-CRP），matrix metallopro－teinase-9（MMP-9），interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α）. Major cardiac adverse events and adverse drug reactions were recorded within 6 months of treatment. The number of patients with atrial fibrillation at each time point before and after treatment was recorded in both groups. Results：Six months after treatment，left ventricular remodeling in patients（LVPWT，IVST，LVMI），left ventricular diastolic function related indicators（IVRT，DT，LAVI，E/E’），cardiac function related indicators（Tei index，NT-proBNP，6-MWT），myocardial fibrosis related indicators（Gal-3，sST2，CTGF，TGF-β1） and inflammatory response indicators（hs-CRP，MMP-9，IL-6，TNF-α） were significantly better than those before treatment（all P＜0.01），the improvement was more significant in the high-dose group（all P＜0.05）. The incidence of major adverse cardiac events in the high-dose group was lower than that in the low-dose group（χ2=4.195，P=0.041）. There was no significant difference in the incidence of adverse drug reactions between two groups（χ2=1.888，P=0.169），and in the existence rate of atrial fibrillation between two groups at each time point after treatment（all P＞0.05）. Conclusion：Compared with the low dose，the high dose of rosuvastatin can effectively improve the ventricular remodeling，ventricular diastolic function and cardiac function in HFpEF patients，and reduce the incidence of major adverse cardiac events without increasing adverse reactions. The anti-inflammatory and anti-myocardial fibrosis of rosuvastatin may be one of its mechanisms.

Abstract:Objective：To explore the association of triglycerides total cholesterol body weight index（TCBI） with coronary artery disease（CAD）and coronary artery calcified plaque burden. Methods：A total of 561 inpatients meeting the inclusion criteria with suspected stable CAD were consecutively enrolled from September 2015 to June 2017 in the Affiliated Hospital of Chengde Medical University. All of them were divided into the CAD group（n=402） and the non-CAD group（n=159） according to coronary computed tomographic angiography results. We collected clinical data，measured coronary artery calcification（CAC），and assessed CAC scores of all the subjects. A binary logistic multivariate regression model was established on the risk factors of CAD，CAC，and CAD with CAC. Results：The area under the curve of TCBI≥1 818.7 was 0.557（P=0.036），and that of TCBI≥1 818.7 combined with CAD classic risk factors，such as male，smoking，hypertension，type 2 diabetes，and ischemic stroke，was 0.726（P＜0.001）. The prevalence of TCBI increased in the CAD group was higher than that in the non-CAD group（P=0.008）. The prevalence of CAD，type 2 diabetes，dyslipi－demia，and CAC incidence in the high TCBI group was higher than that in the low TCBI group（all P<0.05）. TCBI was positively correlated with epicardial adipose tissue volume，pericardial adipose tissue volume，triglyceride-glucose index，and plasma atheroscle－rosis index（all P<0.05）. Besides classic CAD risk factors，TCBI ≥1 818.7 was a newly discovered independent risk factor for CAD，CAC，and CAD with CAC（all P<0.05）. Conclusion：TCBI≥1 818.7 is an independent risk factor for CAD，CAC，and CAD with CAC，which may be a novel marker for the diagnosis and evaluation of CAD.

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Abstract:Objective：To screen potential hub genes and miRNA in the process of heart failure caused by idiopathic dilated cardiomyopathy（IDCM）. Methods：Two gene expression profiling chips from GEO database were included. After eliminating batch effects and normalizing the data，common differentially expressed genes（DEGs） were screened out. Then，gene ontology（GO） analysis and KEGG pathway enrichment analysis were performed. Protein-protein interaction（PPI） network was constructed by STRING online tool. miRNet was used to obtain miRNA targeting DEGs，and miRNA-DEGs network was constructed. And Cytoscape was used to analyze hub genes and miRNA. Results：According to ｜logFC｜ > 0.4 and P < 0.05，a total of 210 DEGs were screened out. These DEGs were mainly enriched in PI3K signaling pathway，MAPK signaling pathway and tumor-related pathways. Hub genes including STAT3，MYC and CCND1 as well as key miRNAs such as miR-34a-5p，miR-17-5p and miR-20a-5p were selected. Conclusion：Based on bioinformatics methods，the hub genes and miRNA in the process of heart failure caused by IDCM were screened and analyzed，a total of 210 differentially expressed genes and 7 functional modules were screened，and 3 key miRNAs were analyzed，which provided ideas for further research on the mechanism and therapeutic targets of IDCM heart failure.

Abstract:Objective：To identify differentially expressed m6A associated genes of oral squamous cell carcinoma（OSCC） and their related signaling pathways，providing candidate biomarkers for diagnosis and potential target genes for therapy of OSCC. Methods：After downloading data on OSCC from TCGA database，m6A related gene expression profiles were obtained. R software was used to select differentially expressed m6A genes between normal samples and tumor ones. Cluster analysis was performed for all m6A associated genes and the interactions among these genes were studied. Gene set enrichment analysis（GSEA） was done for signaling pathways of OSCC due to differentially expressed m6A genes. Results：Eight differentially expressed m6A genes were identified（P<0.05），including HNRNPC，KIAA1429，METTL3，METTL14，RBM15，WTAP，YTHDF1 and YTHDF2. They showed significantly higher expressions in tumor samples compared with normal ones（P<0.05）. There was a complex correlation between m6A related genes. GSEA revealed that the differentially expressed m6A genes were mainly involved in such signaling pathways as cell cycle，base excision repairing，homologous recombination and spliceosome. Conclusion：HNRNPC，KIAA1429，METTL3，METTL14，RBM15，WTAP，YTHDF1 and YTHDF2 are expected to be potential diagnostic markers and molecular therapeutic targets of OSCC patients.

Abstract:Objective：To investigate the expression of Rho GTPase activating protein 44（ARHGAP44） in colorectal tissue and its relationship with the clinicopathological characteristics and prognosis of colorectal cancer in multiple data sets. Methods：With the use of the gene expression omnibus（GEO） and the cancer genome atlas（TCGA） cohorts，we summarized the expression of ARHGAP44 and its relationship with clinicopathological characteristics of colorectal cancer，evaluated the prognostic value by the Cox regression model and Kaplan-Meier curve. The mRNA expression of ARHGAP44 was validated in clinical samples by RT-qPCR. And gene set enrichment analysis（GSEA） was used to predict the pathway. Using the ssGSEA（single-sample gene-set enrichment analysis） algo－rithm，the association between ARHGAP44 and immune cell infiltration was calculated. Results：In TCGA，GEO datasets and clinical samples，the ARHGAP44 expressions were reduced in tumor tissues（P<0.001），and related to T stage and TNM stage（P<0.05）. The low expression of ARHGAP44 showed an independent risk factor for OS（overall survival）of colorectal cancer patients（HR=0.44，P=0.02）. GSEA results showed that the high expression samples of ARHGAP44 were rich in colorectal cancer pathway，Notch pathway，T cell receptor pathway，B cell receptor pathway and other gene sets. The expression of ARHGAP44 was negatively correlated with the infiltration levels of macrophages，T helper cells，TIL（tumor-infiltrating lymphocytes） and typeⅠIFNresponse（cor=-0.35，-0.37，-0.33，-0.35，-0.23 and -0.32，P=0.008，0.006，0.027，0.021，0.041 and 0.021）. Conclusion：The expression of ARHGAP44 is down-regulated in colorectal cancer，which can be used as an independent prognostic biomarker for survival，and has a potential role in tumor immunology.

Abstract:Objective：To explore the expression of SLC11A1 in multiple colon cancer data sets and its relationship with the clinical prognosis，and its potential role in the tumor microenvironment（TME） in colon cancer. Methods：With the use of the tumor genome atlas（TCGA） and the gene expression omnibus（GEO），the expression of SLC11A1 and its relationship with the clinical prognosis of colon cancer were analyzed. At the same time，we used the algorithm of ESTIMATE and the single-sample gene-set enrichment analysis（ssGSEA） algorithm to explore the relationship between SLC11A1 and immune cell infiltration within the TME. Results：In the TCGA and GEO datasets，the expression of SLC11A1 increased in tumor tissues（P<0.001） compared with normal tissues. High SLC11A1 expression was an independent risk factor for the overall survival（OS） of colon cancer patients. In addition，SLC11A1 was strongly linked to the infiltration and aggregation of tumor associated macrophages（TAMs） and M2-type macrophages，and further analysis indicated that it was strongly correlated with increased CD8+ T cell inhibitors TGFB1 and CXCL12 and their receptors. Conclusion：In summary，the expression of SLC11A1 is associated with the function regulation of cells in TME，especially TAMs and other immune cells，and it might have potential as a prognostic biomarker of colon cancer.

Abstract:Objective：To predict the effective components and active mechanism of Artemisiae annuae herba against rheumatism based on network pharmacology. Methods：The main active components of the Artemisiae annuae herba were screened by TCMSP database. Furthermore，the target genes were searched by Gene Cards and OMIM database. Meanwhile，the anti-rheumatic target of active components of Artemisiae annuae herba was obtained by mapping it with the active components targets，and the protein-protein interaction network was constructed through the STRING platform. The anti-rheumatic targets of active constituents of Artemisiae annuae herba were classified by GO and analyzed by KEGG pathway enrichment using DAVID 6.7 database，and the prediction model of artemisia annua herba active constituents-target-signal pathway network was constructed by using Cytoscape 3.6.1 software. Results：In this study，a total of 18 effective components and 84 effective targets were predicted，and its mechanism may be related to B cell receptor signaling pathway，NOD-like receptor signaling pathway，T-cell receptor signaling pathway，Toll-like receptor signaling pathway，etc. Among these pathways，six target genes，PTGS2，RELA，AR，NOS2，AKT1，MAPK1，may play crucial roles. Conclusion：The active ingredients such as kaempferol，quercetin and luteolin may regulate multiple signaling pathways through targets such as PTGS2，RELA，AR，NOS2，AKT1 and MAPK1，to play an anti-rheumatic role.

Abstract:Objective：To find the plasma biomarkers of Acinetobacter baumannii（A. baumannii） infection based on proteomics tech－niques. Methods：Plasma of patients with blood stream infection was collected，and was divided into A.baumannii infection group（n=16），other G- bacteriainfection group（8 cases of K. pneumoniae infection and 8 cases of E. coli infection），and plasma of other 16 healthy people（control group） was collected. The plasma proteins of the above subjects were analyzed by mass spectrometry and bioinformatics to find the specific proteins related to A. baumannii infection. Results：There were 13 proteins in A. baumannii infection which were different from other G- bacteria infections，52 proteins were different from health controls，and 3 differential proteins in the same time（CNDP1，IGLL5，PEDF，all P<0.05）. Data from the GO analysis showed that most differential proteins were in the extracellular region，which were mostly related to coagulation，inflammation and immune response，while KEGG analysis showed that most proteins were involved in the complement and clotting cascade pathway. Conclusion：Changes in plasma proteome of A. baumannii infection were closely related to inflammation，coagulation and immune response. CNDP1，IGLL5 and PEDF may be candidate plasma biomarkers for A. baumannii infection.