Abstract:Objective To screen for differentially expressed genes and proteins by RNA sequencing（RNA-Seq） and tandem mass tag（TMT） proteomics analysis，and to explore the complex pathological mechanism of spinal cord injury（SCI）.Methods Fifty female C57BL/6 mice were equally randomized into two groups： sham group and model group. The SCI model was established by compressing the 1st lumbar vertebra with an aneurysm clip，and spinal cord tissues were harvested 14 days later. The hindlimb locomotor function was assessed by basso mouse scale（BMS）； pathomorphological changes of the injured area of the spinal cord were determined by hematoxylin-eosin（HE） staining； RNA-Seq was used to screen for differentially expressed genes，and TMT proteomics analysis was used to screen for differentially expressed proteins. The two sequencing techniques were combined to screen for the messenger RNAs（mRNAs） and proteins with consistent change trends and perform bioinformatics analysis.Results Compared with the sham group，the model group had significantly decreased BMS scores（P<0.05）. HE staining showed loose and disordered structure，cavitation，karyopyknosis，serious inflammatory infiltration，and neuronal necrosis in the injured area of the spinal cord. A total of 565 differentially expressed mRNAs（including 545 up-regulated mRNAs and 20 down-regulated mRNAs） were screened out by RNA-Seq，and 339 differentially expressed proteins（including 278 up-regulated proteins and 61 down-regulated proteins） were screened out by TMT proteomics analysis. The cluster heat maps of the two sequencing methods showed that the expression patterns of the two groups of samples were very different. A total of 83 up-regulated mRNAs or proteins were obtained by the Venn diagram. Eleven core targets were obtained by the protein-protein interaction network analysis. Gene ontology enrichment analysis showed that molecular functions or biological processes were mainly found in immune response，lysosome pathway，bacterial reaction，and vacuole lysis. Kyoto encyclopedia of genes and genomes analysis showed that enrichment pathways were mainly tuberculosis，lysosome，and phagosome pathways.Conclusion In this study，the 11 mRNAs or proteins identified may be core targets for regulating the pathological process of SCI，and the pathological mechanism may be closely related to immune response，lysosome，and phagosome pathways.

Abstract:Objective To investigate the effects of miR-149-5p in regulating the migration and invasion ability of gastric cancer（GC） cells and the molecular mechanism.Methods The expression of miR-149-5p in GC cell lines and tissue specimens was measured by qRT-PCR to analyze the relationship between miR-149-5p expression and the clinicopathological parameters and prognosis of patients with GC. We separately overexpressed and knocked down miR-149-5p in GC cells to investigate the expression level of miR-149-5p on the migration and invasion ability of GC cells using Transwell assay. The target genes of miR-149-5p were predicted using bioinformatic tools，which were verified using luciferase reporter gene assay.Results The expression of miR-149-5p was significantly down-regulated in both GC tissues and cell lines（P<0.05）. The expression level of miR-149-5p was significantly correlated with the depth of invasion（P=0.016），lymph node metastasis（P=0.001），and TNM stage（P=0.023） of patients with GC. Low expression of miR-149-5p was an independent risk factor for the overall survival of patients with GC. Compared with the control group，miR-149-5p mimics significantly inhibited the migration and invasion ability of gastric cancer cells（P<0.01），while transfection with miR-149-5p inhibitors produced the opposite effects（P<0.05）. miR-149-5p targeted the expression of adipocyte enhancer-binding protein 1（AEBP1）. The luciferase reporter gene assay showed that miR-149-5p significantly inhibited the luciferase activity of the wild-type AEBP1 vector（P<0.01），with no effects on the luciferase activity of the mutant type. Knockdown of AEBP1 could partly reverse the effects of downregulating miR-149-5p on the migration and invasion ability of GC cells.Conclusion miR-149-5p is lowly expressed in GC tissues，which can negatively regulate the migration and invasion of GC cells by targeting AEBP1 protein expression.

Abstract:Objective To explore the feasibility of photoacoustic tomography（PAT） and multispectral optoacoustic tomography（MSOT） for testicular structure and function imaging in a varicocele rat model.Methods Eighteen SD rats were randomly divided into blank group，model group，and Aescuven forte group，with 6 rats in each group. In the model group and Aescuven forte group，a varicocele rat model was established by narrowing the left renal vein and ligating the left spermatic vein. After 4 weeks of intervention，the PAT/MSOT image，oxygen metabolism，and oxidative stress levels of rat left testicle were compared to investigate the testicular imaging characteristics of rats with different interventions.Results PAT/MSOT provided a clear image of rat testicular structure and reflected the level of oxygen metabolism in the testes.Conclusion PAT/MSOT can be used to detect the structural and functional changes in the testis caused by varicocele. PAT/MSOT is a novel，simple，and safe method for non-invasive evaluation of testes.

Abstract:Objective To investigate the effect of Glycyrrhiza uralensis Fisch. endophyte JTZB55 combined with cisplatin on proliferation and apoptosis of human lung cancer A549 cells and the possible mechanism.Methods MTT assay was used to determine the effect of JTZB55 and/or cisplatin on the survival rate of A549 cells. According to the synergistic index，the subsequent experimental concentration was screened out，and A549 cells were divided into normal control group，2 μg/mL cisplatin group，800 μg/mL JTZB55 group，and 2 μg/mL cisplatin + 800 μg/mL JTZB55 group. Flow cytometry was used to determine the effect of drugs in each group on the apoptosis of A549 cells. Western blot was used to measure the expression levels of mitochondrial apoptosis and endoplasmic reticulum stress pathway-related proteins in cells treated with drugs in each group.Results MTT results showed that the survival rate of A549 cells was decreased significantly after cisplatin and JTZB55 were administrated. Compared with the cisplatin group，the cell survival rate in the combination group was significantly decreased（P<0.05），and the synergistic index of 800 μg/mL JTZB55 combined with 2 μg/mL cisplatin was the highest. The results of flow cytometry showed that both JTZB55 and cisplatin could promote the apoptosis of A549 cells（P<0.01），and the apoptosis rate of A549 cells was significantly increased in the combination group（P<0.01）. Western blot results showed that cisplatin combined with JTZB55 could significantly decrease the expression of B cell lymphoma/leukemia-2（Bcl-2） protein and up-regulate the expression of Bcl-2 associated X（Bax），cytochrome C（CytC），cysteine aspartate protease-3（Caspase-3），cysteine aspartate protease-8（Caspase-8），cysteine aspartate protease-9（Caspase-9），glucose regulated protein 78（GRP78），and CCAAT/enhancer-binding protein-homologous protein（CHOP）（P<0.05）.Conclusion JTZB55 can enhance the inhibitory effect of cisplatin on the proliferation of A549 cells and promote the apoptosis of A549 cells. The mechanism may be related to the down-regulation of Bcl-2 and up-regulation of Bax，CytC，Caspase-3，Caspase-8，Caspase-9，GRP78，and CHOP.

Abstract:Objective To investigate the level of milk fat globule-EGF factor Ⅷ（MFG-E8） in plasma and the expression of MFG-E8 protein in the small intestine in rats with obstructive jaundice，and to determine whether supplementation of MFG-E8 can reduce apoptosis and permeability damage of the intestinal mucosa in rats with obstructive jaundice.Methods The rat model of obstructive jaundice was established by common bile duct ligation（BDL）. Thirty-two male adult SD rats were randomly divided into sham operation group（sham group），obstructive jaundice group（BDL group），MFG-E8 treatment group（MFG-E8 group），and treatment control group（vehicle group）. The MFG-E8 group and vehicle group were intraperitoneally injected with MFG-E8（20 μg/kg ） or an equal volume of normal saline on the 1st，3rd，and 5th days after BDL. Blood and tissue samples were collected on the 7th day after modeling. Western blot was used to determine plasma MFG-E8 levels，intestinal MFG-E8 protein expression，and intestinal cleaved caspase-3 levels. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay was used to observe the apoptosis of intestinal cells. Fluorescein isothiocyanate-dextran（FITC-D） was used to assess intestinal mucosal permeability. Enzyme-linked immunosorbent assay was used to determine plasma tumor necrosis factor alpha（TNF-α） levels.Results Compared with the sham group，the BDL group had significantly decreased plasma MFG-E8 level and MFG-E8 protein expression level in the small intestine（5.675±0.638 vs. 3.087±0.272，P=0.001；1.24±0.157 vs. 0.484±0.090，P=0.000）. Compared with the vehicle group，the MFG-E8 group had significantly decreased apoptosis ratio of small intestinal cells and expression of cleaved caspase-3 protein（22.333±5.085 vs. 14.467±3.722，P=0.048； 0.887±0.132 vs. 0.582±0.145，P=0.024）. The FITC-D and plasma TNF-α levels in the vehicle group were significantly higher than those in the sham group（4.515±0.601 vs. 1.519±0.346，P=0.000；233.84±21.21 vs. 78.24±13.75，P=0.000）. The FITC-D level in the MFG-E8 group（2.826±0.588） was significantly lower than that in the vehicle group（P=0.000）. Compared with the vehicle group，the plasma level of TNF-α（109.90±24.06） in the MFG-E8 group was significantly decreased by 53%（P=0.000）.Conclusion MFG-E8 supplementation significantly attenuates apoptosis and permeability damage of the intestinal mucosa，as well as systemic inflammatory responses in rats with obstructive jaundice.

Abstract:Objective To investigate the effect of picroside Ⅱ（PicrⅡ） on learning and memory functions in Alzheimer’s disease（AD） model in vivo and in vitro and autophagy-related mechanisms.Methods Western blot was used to observe the effect of PicrⅡ（12.5-400 μmol/L） on the expression of β-amyloid precursor protein（APP），β-site amyloid precursor protein cleaving enzyme 1（BACE1），presenilin 1（PS1），and carboxyl-terminal fragment of β-amyloid precursor protein（CTF） in the cell model of AD；after co-treatment with PicrⅡ and chloroquine（CQ）（50 μmol/L） or bafilomycin（BafA1）（10 μmol/L），its effect on the expression of APP，BACE1，PS1，C99，sequestosome 1（P62），microtubule-associated protein light chain 3（LC3），and ubiquitinated proteins in cells was observed；immunofluorescence assay was used to analyze autophagic flux. Then a mouse model of Aβ was established，and the mice were divided into WT+PBS group，WT+PicrⅡ group，Aβ+PBS group，and Aβ+PicrⅡ group（20 mg/kg）. The Morris water maze test was used to verify the learning and memory functions of Aβ model mice，and the protein expression levels of P62 and LC3 in the hippocampus of mice were measured.Results Western blot showed that compared with the control group，PicrⅡ（50 μmol/L） significantly reduced the protein expression levels of APP[（60.46±7.97）%，P=0.049] and related metabolites，and it also reduced the expression level of P62[（65.31±3.51）%，P=0.041] and increased the expression level of LC3Ⅱ[（162.01±12.24）%，P<0.001]；with the action of CQ，PicrⅡ could reduce the expression levels of P62[（147.24±10.69）%，P<0.001] and LC3Ⅱ[（826.23±39.18）%，P<0.001]. The analysis of autophagic flux showed that compared with the control group，PicrⅡ increased the total number of fluorescent dots（23.24±1.50，P<0.001） and the number of red dots（3.52±0.33，P<0.001）. The behavioral analysis showed that compared with the WT+Aβ group，the Aβ+PicrⅡ group had significant increases in learning ability（F=17.25，P<0.001） and memory ability（F=6.627，P<0.001），as well as a significant reduction in P62[（176.66±11.47）%，P=0.023] and a significant increase in LC3 Ⅱ[（81.20±3.69）%，P<0.001].Conclusion PicrⅡ can enhance autophagy by promoting autophagosome-lysosome fusion，thereby promoting the clearance of Aβ and improving Aβ-induced learning and memory deficits.

Abstract:Objective To construct a dynamic incubation system of biomimetic urinary flow stress bacteria biofilm by simulating human bladder in vitro，and to explore the application effect of this system.Methods For the dynamic incubation system to construct bacterial biofilm in vitro，we used Escherichia coli as the experimental bacteria and silica gel membrane as the carrier of membrane formation to establish five bionic effects of urinary flow stress： physiological secretion during the day and night，physiological urination during the day， and pathological urination during the day and night. In addition， we set up two circulation scheme groups： physiological group and pathological group to observe the characteristics of biofilm formation of Escherichia coli under the stimulation of bionic urinary flow stress. The detection methods included： counting the living bacteria of biofilm bacteria， fluorescence staining combined with laser confocal microscope observation，determining the average light density value of the fluorescence image of biofilm bacteria，and constructing biofilm stereogram to measure the thickness of biofilm. The average values among multiple groups were detected by using one-way analysis of variance（ANOVA），and LSD-t method was used to compare the average values within groups in pairs.Results Through the comparison of data in each group，with the prolongation of incubation time，the number of viable bacteria of biofilm bacteria increased gradually，and the number of viable bacteria in pathological group at 48 h，static group and physiological group at 60 h increased significantly and reached the peak（^cP=0.000）；The measured values of average light density and biofilm thickness in the fluorescence image of biofilm bacteria increased gradually，and the measured values in the pathological group at 48 h，the static group and the physiological group at 72 h increased significantly and reached the peak（^cP=0.000）；There was no significant difference in other measured values in each group（P>0.01）. Through the comparison of data between groups，compared with the static group，the viable counts of biofilm bacteria in the physiological group and the pathological group at 36 h，48 h，60 h，84 h and 96 h were significantly increased（^aP=0.000）. Compared with the physiological group， the increase in the pathological group was more significant（^bP=0.000）. Compared with the static group， the fluorescence image average light density of biofilm bacteria in the physiological group at 96 h and the pathological group at 48 h and 96 h increased significantly（^aP=0.000），and the measured values of biofilm thickness in the physiological group at 72 h， pathological group at 48 h and 96 h increased significantly（^aP=0.000）. Compared with the physiological group， the measured values of the fluorescence image average light density and biofilm thickness of bacteria in the pathological group at 48 h and 96 h were significantly increased（^bP=0.000）. There was no significant difference in other measured values among the groups（P>0.01）.Conclusion In this study，a dynamic incubation system of bacterial biofilm has been successfully constructed in vitro to simulate human bladder， which has been verified that the biomimetic urinary flow stress can significantly promote the formation of Escherichia coli biofilm.

Abstract:Objective To explore the pathogenesis of intrahepatic cholestasis of pregnancy（ICP） in rats induced by either estradiol or progesterone，through determining the levels of liver damage，bile acid profiles，inflammatory factors，and oxidative stress.Methods ICP models were established by subcutaneous injection of either estradiol or progesterone in the neck of Sprague-Dawley（SD） rats during pregnancy. The models were evaluated by measuring the levels of TBA（serum total bile acid），ALT（alanine aminotransferase），AST（aspartate aminotransferase），and ALP（alkaline phosphatase）. Liver injury was evaluated through pathological section observation. The bile acid profiles of the liver were analyzed by liquid chromatography-mass spectrometry. The transcription levels of inflammatory cytokines（tumor necrosis factor-α[TNF-α]，interleukin[IL]-1β，and IL-6） in the liver were measured using RT-qPCR. The levels of oxidative and antioxidative markers（malondialdehyde[MDA]，glutathione[GSH]，and superoxide dismutase[SOD]） were determined using commercial kits. The mRNA and protein levels of antioxidative stress markers（nuclear factor erythroid 2-related factor 2[NRF2]，glutamate-cysteine ligase modifier subunit[GCLM]，glutamate-cysteine ligase catalytic subunit[GCLC]，NAD（P）H quinone oxidoreductase-1[NQO1]，and heme oxygenase-1[HO-1]） in the liver were determined using RT-qPCR and Western blotting，respectively.Results The levels of serum total bile acid，ALT，AST，and ALP in the estradiol group and progesterone group were significantly higher than those in the control group. HE staining showed narrower hepatic sinusoids and cholestasis in the estradiol group and hepatocyte swelling and mild hepatic steatosis in the progesterone group. The bile acid profiles were significantly different between the groups. In the estradiol group，the levels of TNF-α，IL-1β，IL-6，and MDA in the liver were significantly increased，while the levels of GSH and SOD as well as the mRNA and protein levels of NRF2，GCLM，GCLC and NQO1 were significantly decreased，with no significant changes in the progesterone group.Conclusion Both estrogen and progesterone induce ICP in rats. Estrogen can inhibit the NRF2-GCLM and NRF2-GCLC pathways to aggravate liver injury，while progesterone has no such effect.

Abstract:Objective To explore whether the water extract of Dendrobium nobile Lindl.（DNL） can alleviate learning and memory impairment through synaptic plasticity modulation in APP/PS1 Alzheimer’s disease（AD） model in mice.Methods Twelve wild-type（WT） C57BL/6 male mice were randomly divided into two groups（n=6）：WT control group（WT） and DNL treatment alone group（WT+DNL，DNL was intragastrically administered at 10 mg/kg，once a day，for 2 months）. Twelve APP/PS1 mice were divided into two groups：APP/PS1 AD model group（APP/PS1） and APP/PS1 AD model treatment group（APP/PS1+DNL，APP/PS1 mice aged seven months received DNL intragastrically administered at 10 mg/kg，once a day，for two months）. Morris water maze was used to test the memory ability of the mice；Gorky staining was used to determine the number of dendritic spines in the hippocampus of the brain in the mice；electroencephalography was used to measure the long-term potentiation（LTP） in the hippocampus of the brain； immunohistochemical techniques were used to measure the expression of glial fibrillary acidic protein and ionized calcium-binding adaptor molecule 1 in the hippocampus and evaluate the number of glial cells. The protein expression levels of Synapsin I，Synaptophysin，interleukin-6（IL-6），tumor necrosis factor-α（TNF-α），and interleukin-1β（IL-1β） in the hippocampus of the mice were measured by western blot.Results Compared with the WT group，the APP/PS1 group had a significantly increased escape latency on days 2-6，showing learning and memory dysfunction. In addition，compared with the WT group，the APP/PS1 group showed reductions in the number of dendritic spines and the expression levels of synapse-related proteins Synapsin I and Synaptophysin but noticeable increases in the number of astrocytes and microglial cells and the expression levels of inflammatory factors in the brain tissue. However，DNL treatment improved learning and memory function，inhibited the loss of dendritic spines and the decreases in synapse-related proteins Synapsin I and Synaptophysin in the APP/PS1 mice，alleviated the LTP injury of neurons in the hippocampal CA1 area，increased the numbers of astrocytes and microglial cells，and significantly downregulated the protein expression levels of inflammatory factors IL-6，TNF-α，and IL-1β.Conclusion The water extract of DNL may alleviate learning and memory dysfunction by inhibiting synaptic LTP in APP/PS1 mice.

Abstract:Objective To investigate the regulatory effect of dexmedetomidine（DEX） on lipid metabolism in hepatocytes and the underlying mechanism.Methods Primary mouse hepatocytes were isolated and then treated with free fatty acids for 24 hours to establish a hepatocyte lipid accumulation model. Primary hepatocytes were treated with DEX separately at concentrations of 200 ng/mL，500 ng/mL，and 1 000 ng/mL for 24 hours，and then were collected for Oil Red O staining to observe the level of lipid accumulation. The expression levels of key lipid metabolism genes in hepatocytes were determined by real-time quantitative PCR and Western blot. The expression of α_2A-adrenoceptors at different time points of DEX treatment was measured by real-time quantitative PCR. The protein expression of associated signaling pathways was determined by Western blot.Results The level of lipid accumulation in primary hepatocytes was significantly reduced after treatment with DEX in a dose-dependent manner. The expression levels of key genes related to fatty acid and triglyceride synthesis（including fatty acid synthase，stearoyl-coenzyme A desaturase 1，and peroxisome proliferator-activated receptor γ） were significantly inhibited（P<0.05）. The expression of α_2A-adrenoceptors was firstly increased and then inhibited. In addition，DEX significantly decreased the phosphorylation levels of P65 and P38 in primary hepatocytes（P<0.05）.Conclusion DEX can reduce lipid accumulation in the liver，which may be related to the activation of α_2A-adrenoceptors and the MAPK/NF-κB signaling pathway.

Abstract:Objective To investigate the role of C-X-C motif chemokine ligand 10（CXCL10）/C-C motif chemokine ligand 2（CCL2） signaling pathway in the development of cervical cancer（CC） in tumor microenvironment.Methods In vitro cervical cancer Hela cells were transfected with shRNA（sh-CXCL10） and CXCL10 cDNA（CXCL10） targeting CXCL10 gene，respectively.Cell proliferation was evaluated by EdU analysis and cell invasion was detected by Transwell assay.THP-1 cell differentiation was induced with phorbol 12-myristic acid 13-acetate（PMA）.The cells were co-cultured with Hela cells（5×10⁶ cells） transfected with the lentivirus or plasmid at a ratio of 1∶4 for 48 h.The number of CD206+ macrophages was determined by flow cytometry and STAT3/NF-κB signal expression was measured by Western blot.Results Compared with the NC group，Hela cells with CXCL10 over-expression co-cultured with THP-1 cells showed increased proliferation，migration，and invasion（P<0.05），and the ratio of CD206+ macrophages increased significantly（P<0.05）.Hela cells with CXCL10 knock-down co-cultured with THP-1 cells showed the opposite results.Compared with NC group，the CXCL10 co-culture system showed significant increases in the expression of p-STAT3，p-NF-κB，and CCL2（P<0.05）.Compared with the sh-NC group，the sh-CXCL10 co-culture system showed significant decreases in the expression of p-STAT3，p-NF-κB，and CCL2（P<0.05）.Conclusion Up-regulation of CXCL10 in CC cells promotes M2 polarization of macrophages in tumor microenvironment.The mechanism may be related to activation of STAT3/NF-κB/CCL2 signal transduction pathway in macrophages，which leads to the enhancement of proliferation，migration，and invasion of CC cells.

Abstract:Objective To investigate the effect of exogenous injection of chemokine C-X3-C-motif ligand 1（CX3CL1） on neuronal function in mice with Alzheimer’s disease（AD） and its mechanism.Methods APP/PS1 double transgenic mice were used to establish a model of AD，and the mice were randomly divided into control group，AD model group，AD+PBS group，and AD+CX3CL1 group. The mice in the AD+CX3CL1 group were given intracranial administration of CX3CL1，and those in the AD+PBS group were injected with an equal volume of PBS solvent. The open field test and the Morris water maze test were used to assess the cognitive behavior of mice，and immunofluorescent staining，TUNEL staining，Nissl staining，Western blotting，and RT-PCR were used to analyze hippocampal tissue.Results The AD mice receiving CX3CL1 injection showed an increase in movement distance，but without a significant difference（P=0.189）；however，in the water maze test，the AD+CX3CL1 group showed a significant reduction in the movement distance to reach the submerged platform（P=0.001）. The AD model group had significant increases in the expression levels of gasdermin D and inflammation-related proteins（P<0.05），while CX3CL1 injection significantly reduced the expression levels of these proteins（P<0.05）. The AD model group had significant reductions in the number of Nissl bodies and the expression levels of （synaptosomal-associated protein of 25 kDa（SNAP25），post synaptic density protein 95（PSD95），and vesicle-associated membrane protein 1（VAMP1），while the CX3CL1 group showed significant increases in the number of Nissl bodies（P=0.001） and the expression levels of SNAP25，PSD95，and VAMP1（P=0.043，0.026，and 0.003）.Conclusion Exogenous CX3CL1 injection can improve neuronal function and protect neurons from damage in AD mice by regulating inflammatory factors and reducing pyroptosis.

Abstract:Objective To identify the risk factors for cognitive dysfunction in patients with brain infarction and type 2 diabetes（T2D），and to offer insights for the diagnosis and treatment of cognitive dysfunction.Methods In this retrospective study，data were collected from patients with brain infarction complicated with T2D，who were admitted to Xijing Hospital，Air Force Medical University from January 2010 to December 2021. A total of 191 eligible patients were divided into cognitive dysfunction group（CD group） and non-cognitive dysfunction group（Non-CD group） according to mini-mental state examination. Data were collected on demographic characteristics，clinical features，and biochemical markers.Results The univariate analysis showed significant correlations of cognitive dysfunction with age，education level，degree of dependence，duration of T2D，D-dimer，and fibrinogen degradation products（P<0.05）. The multivariate logistic regression and receiver operating characteristic curve showed that age（OR=2.249，95%CI=1.172-4.319，P=0.015） and education level（OR=0.587，95%CI=0.352-0.978，P=0.041） were significant risk factors for cognitive dysfunction in patients with brain infarction and T2D.Conclusion Age and education level are tightly related to brain infarction and T2D，and are risk factors for cognitive dysfunction.

Abstract:Objective To perform a retrospective analysis of the clinical outcome of patients with endometrial stromal sarcoma（ESS） and to identify prognostic factors.Methods A total of 82 ESS patients were recruited from June 2009 to March 2020 in The First Affiliated Hospital of Chongqing Medical University. Their clinical data were collected and analyzed，including age，body mass index，menopausal status，tumor size，lymph node resection，ovarian preservation，histological type，stage，adjuvant therapy，and CD10. All patients were followed up. Statistical analysis was performed using SPSS 25.0，the Kaplan-Meier method and log-rank test were used for survival analysis，and Cox regression was used to analyze the prognostic factors.Results A total of 82 patients with ESS were enrolled in this study. The follow-up time was 2-132 months，and the 5-year cumulative overall survival rate was 71.8%. Univariate analysis showed that menopausal status，ovarian preservation，histological type，stage，and indices（ER，PR，and Ki-67） were significantly correlated with progression-free survival. While menopause status，tumor size，histological type，stage，and indices（ER，PR，and Ki-67） were significantly correlated with overall survival. Histological type and ovarian preservation were independent prognostic factors for progression-free survival（P=0.010，0.013），while histological type was an independent prognostic factor for overall survival（P=0.033）.Conclusion There are many factors that affect the prognosis of ESS，and histological type and ovarian preservation are independent prognostic factors for ESS.

Abstract:Objective To investigate the moderate or high risk of atherosclerotic cardiovascular disease（ASCVD） in the health check-up population based on body composition and physical fitness indicators，to develop a risk assessment tool for ASCVD，and to provide reasonable suggestions for the prevention and intervention of ASCVD.Methods Physical examination data were collected from 1 620 individuals，aged 35-59 years，who underwent physical examination in The Second Affiliated Hospital of Chongqing Medical University from July 2020 to January 2022，among whom 1200 were included in the modeling set and 420 were included in the validation set. The risk of ASCVD in the respondents was calculated according to the color diagram of the 10-year risk assessment process of ASCVD. A risk assessment model for ASCVD was constructed based on body composition and physical fitness indicators，and then internal validation was performed for the model. Finally the model was assessed in terms of discriminatory ability，consistency，and clinical practicability.Results In this study，the risk assessment model for ASCVD was established as Logit（P）=-0.298+2.151×sex+0.046×mean liver fat - 0.016×mean bone mineral density + 0.005×abdominal muscle area +0.330×visceral adipose tissue/subcutaneous adipose tissue ratio -0.027×grip strength values of body mass index -0.025×balance force. This model had a specificity of 74.9%，a sensitivity of 81.2%，and an area under the ROC curve of 0.845（95%CI：0.820-0.865）.Conclusion Although the risk assessment model for ASCVD established in this study has good discriminatory ability and clinical practicability，it lacks a good calibration degree in different datasets，and therefore，it is necessary to expand the sample size in the future to further improve the model.

Abstract:Objective To investigate the value of vestibular evoked myogenic potentials（VEMPs） and electrocochleography（EcochG） in predicting the prognosis of acute low-frequency sensorineural hearing loss（ALFHL）.Methods Medical records were collected from 60 patients with first-time unilateral ALFHL who were hospitalized in Department of Otolaryngology，The First Affiliated Hospital of Chongqing Medical University from January 2020 to June 2022. All patients underwent the examinations of EcochG，ocular vestibular evoked myogenic potentials（oVEMP），and cervical vestibular evoked myogenic potentials（cVEMP） after admission，and pure tone audiometry was performed before treatment and at 1 week，2 weeks，and 1 month after treatment. An EcochG SP/AP ratio of >0.4 and an SP/AP area ratio of >1.92 were considered abnormal. If cVEMP and oVEMP were not elicited or if the amplitude ratio was ≤1.61 and the asymmetry ratio was ≤0.29，it was considered abnormal. The chi-square test，the McNemar test，and the Mann-Whitney test were used for comparison of rates between groups，and univariate and multivariate binary logistic regression analyses were used to identify the risk factors for the prognosis of ALFHL.Results The abnormal rates of EcochG，cVEMP，and oVEMP were 26.7%，23.3%，and 35.0%，respectively，in the 60 patients with ALFHL. The abnormal rates of EcochG and oVEMP showed no correlation with age，sex，hearing loss level，starting time of treatment，aural fullness，and dizziness. The abnormal rate of cVEMP in the ALFHL patients with hearing loss >40 dB HL was significantly higher than that in the patients with hearing loss ≤40 dB HL（P<0.05）. The patients with abnormal EcochG，cVEMP，and oVEMP had a significantly lower degree of hearing improvement than those with normal results（P<0.05）. The univariate binary logistic regression analysis showed that abnormal EcochG，cVEMP，and oVEMP were risk factors for non-recovery of ALFHL（all P<0.05），while the multivariate binary logistic regression analysis showed that cVEMP and oVEMP were independent risk factors for non-recovery of ALFHL（both P<0.05）.Conclusion Abnormal cVEMP and oVEMP are independent risk factors for non-recovery of ALFHL，which may be associated with the difficulty in the regression of hydrops in the saccule and utricle of ALFHL patients. The detection of abnormal cVEMP and oVEMP indicates a high risk of non-recovery in patients with ALFHL.

Abstract:Objective To explore the surgical method for papillary thyroid carcinoma（PTC） with 1-2 central lymph node metastases during surgery.Methods A total of 290 patients with PTC and 1-2 central lymph node metastases during the primary surgery in the Department of Breast and Thyroid Surgery of The First Affiliated Hospital of Chongqing Medical University were collected from January 2013 to December 2018，and their clinicopathological features were analyzed.Results The incidence of surgical complications in the hemithyroidectomy（HT） group was significantly lower than that in the total thyroidectomy group（P<0.001）. The surgical method did not affect the occurrence of structurally persistent/recurrent diseases（HR=2.848，95%CI=0.321-25.294，P=0.348）. The independent risk factors for total number of lymph node metastases >5 were tumor diameter >14.5 mm（OR=5.838，95%CI=2.295-14.850，P<0.001），tumor location in the upper part（OR=2.765，95%CI=1.154-6.624，P=0.023），and metastasis to zone Ⅲ lymph nodes（OR=17.310，95%CI=7.053-42.481，P<0.001）.Conclusion The current results support that the preferred surgical method for patients with PTC and 1-2 central lymph node metastases during surgery is HT. If the tumor is located in the upper part and has a diameter of more than 14.5 mm，it is recommended to undergo prophylactic ipsilateral neck lymph node dissection in zone Ⅲ.

Abstract:Objective To improve clinicians’ knowledge of ovarian cysts in children by analyzing the clinical data of 158 children with ovarian cysts.Methods The clinical data of 158 children with ovarian cysts admitted to the outpatient department of Chengdu Women’s and Children’s Central Hospital from September 2019 to September 2022 were collected and divided into three groups according to age（0-1 year，>1-8 years，and >8-16 years）. The clinical characteristics of these patients were analyzed.Results Most of the neonatal and infant ovarian cysts were identified incidentally. Most of the clinical symptoms，including breast development（81.3%） and vaginal bleeding（25.0%），were found in girls aged >1-8 years. Irregular menstruation（45.6%） and abdominal pain（33.3%） were the most common symptoms in girls aged above 8 years. Most of the patients were diagnosed with simple ovarian cysts，but six patients were found to have ovarian tumors. Ovarian torsion was found in 7 cases. Most of the ovarian cysts resolved spontaneously during the follow-up，but some recurred in the children. Central precocious puberty was observed in two children during the follow-up. The surgical intervention rate was 8.9%.Conclusion For most of the girls，no intervention is needed due to their diagnosis with simple cysts； however，some of them may have underlying disease causes or complications such as ovarian tumors，McCune-Albright syndrome，Van Wyk-Grumbach syndrome，central precocious puberty，and ovarian torsion. Hence，these patients should be evaluated carefully and treated with multidisciplinary approaches.

Abstract:Objective To explore the intervention effects of intensive care unit（ICU） diaries in patients with acute stress disorder（ASD） in ICU.Methods Fifty-two patients in ICU were selected and randomly divided into experimental group and control group. The control group received conventional nursing approaches，while the experimental group had ICU diary intervention in addition to the nursing approaches of the control group. The two groups were compared in the incidence of ASD，the Stanford Acute Stress Reaction Questionnaire（SASRQ） score，and the scores of SASRQ dimensions on day 3，7，and 21 after intervention.Results On day 3，there were no significant differences between the experimental group and the control group in the SASRQ score and the incidence of ASD（P=0.075，P=0.471）；the experimental group showed significantly lower scores of dissociation and functional impairment dimensions than the control group（P=0.035，P=0.016），with no significant differences in the three dimensions of re-experience，avoidance，and irritation（P=0.320，P=0.140，P=0.137）. On day 7，significant differences were observed between the two groups in the SASRQ score（49.77±12.16 vs. 76.88±17.56），the incidence of ASD（69.23% vs. 96.15%），and the score of each SASRQ dimension（P=0.000，P=0.028，P=0.000）. On day 21，the two groups also showed significant differences in the SASRQ score（35.92±5.54 vs. 75.23±17.28），the incidence of ASD（38.46% vs. 76.92%），and each SASRQ dimension score（P=0.000，P=0.005，P=0.000）. On days 7 and 21，the SASRQ score，the incidence of ASD，and SASRQ dimension scores were all significantly lower in the experimental group than in the control group.Conclusion ICU diaries can help reduce the symptoms and incidence of ASD in ICU patients.