Abstract:Objective Delayed diabetic wound healing is characterized by dysfunction of multiple cells and formation of neutrophil extracellular traps（NETs）. Previous studies demonstrated that human amniotic epithelial cells（hAECs） can promote diabetic wound healing through regulating fibroblasts，macrophages，and vascular endothelial cells. Whether hAECs have any impact on NETs remains unknown. Our research aims to clarify the effect of hAECs on NETs.Methods Wounds were created on the dorsa of streptozotocin（STZ）-induced diabetic rat models. The wounds were treated with hAECs suspension or PBS and then observed for healing rates on days 5 and 10. Meanwhile，NETs-related biomarkers in the wound tissue were measured. Human neutrophils were extracted and stimulated with phorbol 12-myristate 13-acetate to establish an in vitro model of NETs. The neutrophils were then treated with conditioned medium for human amniotic epithelial cells（CM-hAECs） at 24 and 48 h to observe its impact on NETs production.Results The wound healing rates were significantly higher for wounds treated with hAECs suspension than for those treated with PBS on days 5［（32.61±2.48）% vs.（20.80±2.70）%，P=0.028］ and 10 ［（86.35±0.92）% vs.（61.52±3.70）%，P<0.001］，with an optimal concentration of 5×10⁵/mL. The hAECs suspension-treated group had reduced expression of NETs-related proteins Cit-H₃ and NE in the wound tissue on days 5 and 10. The in vitro study showed that CM-hAECs can inhibit NETs production.Conclusion This study suggests that hAECs may promote diabetic wound healing by inhibiting NETs production.

Abstract:Objective To investigate the effect of XL413，a drug inhibiting cell division cycle 7（CDC7），on colorectal cancer cells and its mechanism of action.Methods Multiple colorectal cancer cell lines were treated with normal saline and XL413 at different concentrations for 72 hours. CCK-8 assay was used to measure the absorbance of cells at 450 nm and calculate cell viability and the half-maximal inhibitory concentration（IC₅₀） of XL413；Annexin V-FITC/PI double staining was used to measure cell apoptosis；colony formation assay was used to observe the regenerative ability of cells；RNA-seq was used to observe the effect of XL413 on cell transcriptome；qRT-PCR was used to measure the relative mRNA expression levels of apoptosis-related genes；Western blot was used to measure the phosphorylation level of minichromosome maintenance protein 2（MCM2），a member of the DNA unwinding minichromosome maintenance protein complex family，and the expression levels of apoptosis-related proteins.Results Compared with the control group，the XL413 treatment group had significant reductions in the abilities of colorectal cancer cell proliferation，migration，and colony formation（P=0.001 5） and a significant increase in the proportion of cells in the early and late stages of apoptosis（P=0.000 6）. As for the transcriptional expression level of genes，the XL413 treatment group had a significant reduction in the ratio of the cell apoptosis markers B-cell lymphoma-2（Bcl-2） to Bcl-2-associated X（P=0.008 7），and at the protein level，XL413 inhibited the phosphorylation of the DNA unwinding-related protein MCM2 and thus DNA replication and promoted the increase in the expression of apoptosis-related proteins.Conclusion XL413 can inhibit DNA replication by reducing the phosphorylation of the DNA unwinding-related protein MCM2 and induce the apoptosis of colorectal cancer cells. The study provides a theoretical basis for the application of XL413 in the treatment of colorectal cancer in the future.

Abstract:Objective To observe the protective effect of remifentanil on intervertebral disc of rats with intervertebral disc degeneration （IDD），and to explore its mechanism of action.Methods A total of 40 healthy SD rats were randomly divided into sham-operation group，model group，low-dose，middle-dose and high-dose remifentanil groups，8 cases in each group. IDD models were constructed by fibrous annulus puncturing. After modeling，low-dose，medium-dose and high-dose remifentanil groups were given intravenous injection of remifentanil through tail vein at rates of 0.2，0.6 and 1.2 μg/（kg·min） for 30 minutes，while model group and sham operation group were given the same volume of normal saline. The pathological changes of intervertebral disc tissues were observed by hematoxylin-eosin（HE） staining. The levels of serum tumor necrosis factor α（TNF-α），interleukin 6（IL-10） and interleukin 1β（IL-1β） were detected by enzyme linked immunosorbent assay（ELISA）. The expressions of peroxisome proliferator-activated receptor-1α（PGC-1α），mitochondrial transcription factor A（TFAM），cysteine aspartic protease-1（Caspase-1），gasdermin D（GSDMD），NOD-like receptor family pyrin domain containing 3（NLRP3），hypoxia-inducible factor 1α（HIF-1α） and vascular endothelial growth factor（VEGF） in intervertebral disc tissues were detected by Western blot.Results Compared with sham operation group，nucleus pulposus was shrunk，arrangement of fibrous annulus was disordered and the number of nucleus pulposus cells was decreased in model group. The levels of serum TNF-α，IL-6 and IL-1β were increased（P<0.05）. The expression levels of PGC-1α and TFAM in intervertebral disc tissues were decreased，and expression levels of Caspase-1，GSDMD，NLRP3，HIF-1α and VEGF were increased（P<0.05）. Compared with model group，disorders of fibrous annulus were significantly improved in medium-dose and high-dose remifentanil groups，and number of nucleus pulposus cells was significantly increased. The levels of serum TNF-α，IL-6 and IL-1β were decreased（P<0.05）. The expression levels of PGC-1α and TFAM in intervertebral disc tissues were increased，and expression levels of Caspase-1，GSDMD，NLRP3，HIF-1α and VEGF were decreased（P<0.05）.Conclusion Remifentanil can improve IDD in rats，and its mechanism is related to inhibiting inflammatory response and pyroptosis of intervertebral disc，and regulating HIF-1α/VEGF signaling pathways.

Abstract:Objective To investigate the role of sodium aescinate in improving and protecting against hydronephrosis injury in mice after unilateral ureteral obstruction（UUO）.Methods A total of 24 healthy male WTC57BL/6 mice，aged 8 weeks，were divided into sham-operation group（Sham group），model group（UUO group），and sodium aescinate group（UUO+sodium aescinate group），with 8 mice in each group. The UUO model was the most commonly used model for the study of hydronephrosis，and the ureter at one side was ligated with silk thread through a lumbar incision（the ureter at one side was ligated and the kidney at the contralateral side was removed to ensure unilateral hydronephrosis and rule out the influence of normal compensation of the kidney at the contralateral side） to establish a mouse model of hydronephrosis after UUO. After corresponding treatment was given for each group，an automatic biochemical analyzer was used to measure the levels of serum creatinine（Scr） and blood urea nitrogen（BUN）；related kits were used to measure the content of tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β），superoxide dismutase（SOD），and malondialdehyde（MDA）；histopathological sections were prepared and then HE staining was used to observe renal histopathological changes under a light microscope and determine the corresponding pathological injury score；the TUNEL method was used to measure the level of cell apoptosis.Results Compared with the Sham group，the UUO group had significant increases in the levels of Scr（21.49±2.87 vs. 11.21±1.79），BUN（19.80±2.55 vs. 9.40±1.78），TNF-α（20.06±1.32 vs. 10.18±0.56），IL-1β（9.65±0.79 vs. 3.46±0.59），and MDA（26.01±1.02 vs. 15.12±1.15），the level of cell apoptosis（150.02±14.78 vs. 86.82±5.66），and renal pathological injury score（3.00±0.46 vs. 0.00±0.00）（P=0.000），as well as a significant reduction in the level of SOD（95.63±3.13 vs. 143.66±5.44，P=0.000）. Compared with the UUO group，the UUO+sodium aescinate group had significant reductions in the levels of Scr（15.94±0.74 vs. 21.49±2.87），BUN（13.08±0.85 vs. 19.80±2.55），TNF-α（15.01±0.90 vs. 20.06±1.32），IL-1β（5.62±0.65 vs. 9.65±0.79），and MDA（19.12±1.08 vs. 26.01±1.02），the level of cell apoptosis（103.97±10.33 vs. 150.02±14.78），and renal pathological injury score（1.57±0.36 vs. 3.00±0.46）（P=0.000），as well as a significant increase in the level of SOD（121.04±3.61 vs. 95.63±3.13，P=0.000）.Conclusion Sodium aescinate exerts a protective effect against hydronephrosis injury after UUO by exerting an anti-inflammatory effect，inhibiting oxidative stress response，and showing an anti-exudation effect to increase vascular tension，and therefore，it becomes a potential treatment option.

Abstract:Objective To investigate the mechanism of carvacrol alleviating oxidative stress damage in rats with diabetic nephropathy （DN） via the SIRT1/FOXO1 pathway. Methods A rat model of DN was established by high-fat diet and intraperitoneal injection of streptozotocin，and then DN rats were divided into control group，model group，L-XQF group（5 mg/kg），M-XQF group（10 mg/kg），and L-XQF group（20 mg/kg）. Fasting blood glucose（FBG），blood urea nitrogen（BUN），serum creatinine（SCr），and urinary microprotein （U-mAlb） were measured. After the rats were sacrificed and renal tissue samples were collected，HE staining and Masson staining were used to observe the pathological changes and fibrosis of renal tissue；ELISA was used to measure the levels of malondialdehyde （MDA），superoxide dismutase（SOD），and glutathione peroxidase（GSH-Px） in renal tissue； Western blot was used to measure the expression levels of SIRT1/FOXO1 pathway-related proteins. Results Carvacrol reduced the levels of FBG，BUN，SCr，U-mAlb，and MDA，improved the pathological injury and fibrosis of renal tissue，and increased the content of SOD and GSH-Px and the levels of SIRT1 and FOXO1 proteins. Conclusion Carvacrol may inhibit oxidative stress in renal tissue of DN rats via the SIRT1/FOXO1 pathway，thereby alleviating renal injury and improving renal fibrosis.

Abstract:Objective To investigate the effect and mechanism of andrographolide（ANDRO） on intervertebral disc nucleus pulposus cell（NPC） apoptosis induced by tert-butyl hydroperoxide（TBHP）.Methods The cell model of NPCs induced by TBHP was established. NPCs were divided into five groups： control group，model group（100 μmol/L TBHP），low-dose ANDRO group（100 μmol/L TBHP+9 μmol/L ANDRO），middle-dose ANDRO group（100 μmol/L TBHP+18 μmol/L ANDRO），and high-dose ANDRO group（100 μmol/L TBHP+36 μmol/L ANDRO）. Cell apoptosis and reactive oxygen species（ROS） levels were determined by flow cytometry. The levels of oxidative stress related indicators，such as superoxide dismutase（SOD），glutathione peroxidase（GSH-PX），and catalase（CAT），were determined by an enzyme-linked immunosorbent assay kit. Mitochondrial membrane potential was measured by a JC-1 probe. Western blot was used to determine the expression levels of dynamin-related protein 1（Drp1），phosphorylated Drp1（p-Drp1），and mitofusin 2（Mfn2）.Results Compared with the control group，the model group had significantly increased apoptosis rate of NPCs，ROS level，and proportion of cells with decreased mitochondrial membrane potential（all P=0.000），significantly decreased SOD，GSH-PX，and CAT levels（all P=0.000），a significantly up-regulated p-Drp1/Drp1 level（P=0.000），and significantly down-regulated protein expression of Mfn2（P=0.000）. Compared with the model group，the high-dose ANDRO group had significantly decreased apoptosis rate of NPCs［（41.37±0.84）% vs.（26.36±1.33）%］，ROS level［（42.62±0.71）% vs.（22.50±0.37）%］，and proportion of cells with decreased mitochondrial membrane potential［（43.85±0.71）% vs.（24.96±1.04）%］（all P=0.000），significantly increased SOD［（288.16±14.59） ng/mL vs.（658.87±13.22） ng/mL］，GSH-PX［（6.66±0.37） ng/mL vs. （15.66±0.56） ng/mL］，and CAT［（40.89±1.04） pg/mL vs. （87.91±1.56） pg/mL］ levels（all P=0.000），a significantly down-regulated p-Drp1/Drp1 level（0.96±0.05 vs. 0.63±0.01）（P=0.001，0.000），and significantly up-regulated protein expression of Mfn2（0.39±0.01 vs. 0.64±0.02）（all P=0.000）.Conclusion ANDRO can inhibit TBHP-induced NPC apoptosis，and the mechanism may be related to the regulation of mitochondrial function and the relief of oxidative stress.

Abstract:Objective To screen drugs that can initiate cellular autophagy among 36 selected monomers of traditional Chinese medicine， and to explore the effects of Bakuchiol on mouse vascular smooth muscle cell autophagy and calcification.Methods The human brain glioma cells（U87） were treated at different concentrations by flavonoids extracted from Chinese herbal medicine for 72 hours， the semi-inhibition concentration（IC₅₀） of U87 cells were treated by MTT method，and U87 cells were treated at different concentration gradients under conditions less than IC₅₀ concentration. The activation of cellular autophagy activity was observed by observing the fluorescent signals of U87 cell GFP-LC3［microtube-related protein 1 light chain 3（LC3）］ and the method of Western blots（WB） to detect autophagy activity and to screen for drugs that can initiate autophagy. Further experiments were conducted to explore the effects of Bakuchiol initiation on vascular smooth muscle cell calcification after autophagy. The calcification model of vascular smooth muscle cells（VSMCs） in mice was constructed using β-GP，involving control group（CTR） and calcification group（CAL），and the expression of Runx2，BMP2 and LC3 was detected by WB. After intervention with Bakuchiol， they were divided into 4 groups：CTR，CAL，calcification with Bakuchiol group（CAL + Administration），and control with Bakuchiol group （CTR + Administration），respectively，with WB detection for LC3，Runx2 and BMP2，and with Alizarin red to observe the formation of cell calcification nodules. In animal experiments，a high-fat diet was used to build a calcification model of the aorta in mice using vitamin D through intraperitoneal injection，and interventions were made in mice using intraperitoneal injection of Bakuchiol. The mice were divided into four groups：CTR，CAL，CTR + Administration and CTR + Administration. The deposition calcium in the artery medium membrane was observed via Von Kossa staining，and the thickness of the membrane in the aorta in mice was observed using the hematoxylin-eosin（HE） staining method.Results The observed fluorescence intensity and WB cues of LC3 showed that：Asarinim，Bavachin，Juncusol，Mullberrin，Ziyuglycoside 2，Bakuchiol，Corylifolinin，Schisandrin A，and Schisandrin B could significantly increase the expression of autophagy（P<0.05）. WB results suggested a significant increase in the expression of BMP2，Runx2，and LC3 in calcified vascular smooth muscle cells（P<0.05）. WB results after intervention with Bakuchiol suggested a decrease in BMP2 and Runx2 expression（P<0.05），and an increase in expression of LC3-Ⅱ（P<0.05）. The results showed a significant decrease in calcium nodule deposition（P<0.05）. In animal experiments，the results of HE staining after the intervention of Bakuchiol showed a significant increase in the thickness of the medium membrane than the CAL group，and the Von Kossa results hinted that the Bakuchiol could significantly reduce calcium deposition.Conclusion Bakuchiol can activate the autophagy of VSMCs and affect the process of aortic calcification in mice.

Abstract:Objective To explore the diagnostic role of flow cytometry（FCM） immunophenotyping and the clinical and laboratory characteristics of aggressive NK-cell leukemia（ANKL）.Methods A retrospective analysis was performed on clinical data of 11 patients diagnosed of ANKL in West China Hospital from January 2019 to December 2019. Bone marrow cytomorphology，immunophenotype and pathological findings were collected and analyzed.Results All patients suffered from fever accompanied with hemophagocytic lymphohistiocytosis（HLH）. Hemophagocytic cells were found in bone marrow smears of 8 cases. FCM revealed an immunophenotype of CD2⁺sCD3^-CD4^-CD8^-CD56⁺CD19^- in all 11 cases of ANKL. The expression of CD45 and CD56 increased（P=0.048），and the volume of cells was relatively large（P=0.000）. CD16^- was found in 5 patients，and partial or complete loss of CD7 expression was identified in 6 patients. Immunohistochemical tests were performed on 8 patients，and the results were CD3ε⁺，granzyme B⁺，and EBER1/2-ISH⁺.Conclusion The immunophenotype of ANKL has unique characteristics and is of great significance in the diagnosis and differential diagnosis of ANKL. Timely and differential diagnosis of ANKL can be achieved by comprehensive analysis of clinical characteristics， bone marrow cell morphology，immunophenotype and immunohistochemistry.

Abstract:Objective To develop a quantitative test kit for determining hepatitis B core-related antigen（HBcrAg），and to evaluate its performance.Methods A chemiluminescent immunoassay for HBcrAg was established by studying the raw antibody，optimizing the preparation process，and adjusting the reaction system，and after the kit was assembled，recombinant HBcrAg and clinical serum were used to evaluate its detection performance. IBM SPSS Statistics 20 software was used for data processing.Results The standard curve of the kit was y=143.37x+344.91 （R²=0.999 8），with a detection sensitivity of 1.43 pg/mL and a reference interval of ≤25.595 pg/mL for healthy people. Serum samples were tested for 208 patients with chronic hepatitis B，and serum HBcrAg level was above this critical value for all 18 serum samples with an HBV DNA level of 10⁸-10⁴ IU/mL； for the 26 serum samples with an HBV DNA level of 10³-10² IU/mL，24（92.31%） tested positive for serum HBcrAg； for the 164 serum samples with HBV DNA below the limit of detection，73 （44.51%） tested positive for serum HBcrAg.Conclusion This kit can determine the serum level of HBcrAg in patients with chronic hepatitis B and thus has an important application value in monitoring the treatment outcome of patients with chronic hepatitis B and correctly judging the endpoint of treatment.

Abstract:Objective To investigate the survival status of patients living with human immunodeficiency virus（HIV）/acquired immunodeficiency syndrome（AIDS） receiving antiretroviral therapy in the integrated HIV prevention and treatment demonstration zone of Chongqing，China，and to explore the factors influencing patients’ survival.Methods A retrospective cohort study was performed to analyze the survival of patients aged ≥18 years who received antiretroviral treatment for the first time in the demonstration zone from 2016 to 2021. The survival rate was calculated by using the life-table method. The risk factors affecting death were analyzed using a Cox proportional hazards model.Results A total of 21 506 subjects were included in the study. A total of 54 919.63 person-years were followed up. The all-cause mortality rate was 3.67/100 person-years. The cumulative survival rates of 12，36，and ≥72 months since the initiation of antiretroviral therapy were 94.09%，88.65%，and 82.98%，respectively. The multivariable Cox proportional hazards regression analysis showed that the death risk of women was 0.54 times that of men. The risk of death for patients with treatment initiated at age of ≥60 years was 4.38 times that of those with treatment initiated at age of 18-30 years. The death risk of homosexually transmitted infection was 0.65 times that of heterosexually transmitted infection. In terms of the baseline number of CD4⁺ T cells，the risks of death in the groups of 200-349 cells/μL and ≥350 cells/μL were 0.61 and 0.49 times that of the group of 0-199 cells/μL，respectively. In terms of the interval between diagnosis and treatment，compared with the group of <30 days，the groups of 31-90 days，91-365 days，and >365 days were 1.25，1.56，and 1.74 times more likely to die of HIV/AIDS，respectively. The death risks with other first-line treatments and second-line treatments were 1.47 and 1.65 times that with standard first-line treatments，respectively.Conclusion The mortality rate is decreased in patients with HIV/AIDS receiving antiretroviral therapy in the Chongqing demonstration zone. Early detection and treatment of HIV/AIDS should be strengthened for patients with a high risk of death，those with treatment beginning at age of ≥60 years，a low baseline CD4⁺ T cell count，and a long interval between diagnosis and first treatment.

Abstract:Objective To investigate the clinical effect of plasma exchange（PE） combined with double plasma molecular adsorption system（DPMAS） in patients with liver failure and hyperbilirubinemia after the first and second times of treatment.Methods A retrospective analysis was performed for 57 patients with liver failure who attended our hospital from January 2020 to May 2022，and according to artificial liver support therapy，24 patients who received PE were enrolled as control group，while 33 patients who received PE+DPMAS were enrolled as experimental group. Clinical data were collected from the two groups of patients with liver failure and hyperbilirubinemia before and after two times of treatment，and the two groups were observed and compared in terms of the changes in liver function，coagulation function，nutritional status，and other indicators after treatment.Results Both the experimental group and the control group had significant changes in total bilirubin（TBil），alanine aminotransferase（ALT），aspartate aminotransferase（AST），globulin（Glb），and fibrinogen after the first and second times of treatment（P<0.05），but the experimental group showed better clearance of TBil，ALT，and AST compared with the control group，and there was no significant difference in TBil between the experimental group and the control group before treatment［（388.37±180.26） μmol/L vs. （382.37±151.39） μmol/L，P>0.05］，after the first time of treatment［（296.22±137.81） μmol/L vs. （310.76±113.57） μmol/L，P>0.05］，and after the second time of treatment［（244.63±143.40） μmol/L vs. （293.91±149.77） μmol/L，P>0.05］. There was no significant change in albumin in the experimental group before and after two times of treatment（P>0.05），while there was a significant change in albumin in the control group（P<0.05）；there was no significant change in international normalized ratio（INR） in the experimental group before and after two times of treatment（P>0.05），while there was a significant change in INR in the control group（P<0.05）. There were significant differences between the two groups in prealbumin，albumin，and fibrinogen after the first time of treatment（P<0.05），and there were no significant differences in TBil，platelet count，Glb，ALT，and AST between the experimental group and the control group at different time points of treatment（P>0.05）.Conclusion The therapeutic paradigm of PE+DPMAS for two times is more effective in adsorbing and reducing bilirubin and aminotransferases in patients with liver failure，with little impact on coagulation function and albumin，and therefore，it is an effective method for the treatment of patients with hyperbilirubinemia.

Abstract:Objective To investigate the feasibility and safety of hysteroscopic tissue removal system in the treatment of interstitial ectopic pregnancy.Methods Related clinical data were collected from 16 patients with unruptured interstitial ectopic pregnancy who were admitted to Department of Gynecology，Jiangxi Maternal and Child Health Hospital，from April 2019 to April 2022 and were treated by the hysteroscopic tissue resection system，and a preoperative analysis was performed for preoperative blood β-human chorionic gonadotropin（β-HCG），ultrasound findings，time of operation，intraoperative blood loss，and time for blood β-HCG to return to normal after surgery.Results The 16 patients with unruptured interstitial ectopic pregnancy had a preoperative blood β-HCG level of 830-31 153 mIU/mL，with a mean value of （8 643.561±1 752.000） mIU/mL，and pregnancy tissue on ultrasound had a maximum size of 33 cm×28 cm and a minimum size of 11 cm×10 cm. All patients had a confirmed diagnosis of interstitial ectopic pregnancy after microscopic examination，and hysteroscopic tissue resection system was used to remove the lesions of interstitial pregnancy，among whom 2 patients were converted to laparoscopy. The mean time of operation was 34（20，92） minutes，and the mean volume of intraoperative blood loss was 46.6（10，250） mL. The surgical process was smooth with no obvious complications. The patients had a mean hospital stay of 2 days and a time of （22.00±5.11） days for blood β-HCG to return to normal after surgery. Ultrasound examination at 1 month after surgery showed no residue of pregnancy tissue，and 5 patients became pregnant successfully during follow-up for half a year.Conclusion The hysteroscopic tissue resection system can preserve organ integrity in the treatment of unruptured interstitial ectopic pregnancy.

Abstract:Objective To investigate the electrophysiological features of patients with segmental zoster paresis.Methods A retrospective analysis was performed for the neural electrophysiological data of 11 patients who attended Electromyography Examination Room from October 2017 to October 2022.Results All 11 patients had abnormal electrophysiological results，and the abnormal rate of nerve conduction was 20.9%（41/196）. The abnormal rates of motor nerve conduction and sensory nerve conduction were 26.0%（26/100） and 15.6%（15/96），respectively，mainly reduction in the amplitude of the nerve involved. Compared with the contralateral side，the ipsilateral side had an abnormal rate of nerve conduction amplitude of 18.4%（36/196），with an abnormal rate of 23.0%（23/100） for motor nerve conduction amplitude and 13.5%（13/96） for sensory nerve conduction amplitude. INCAT score was positively correlated with the maximum reduction ratio of CMAP amplitude（P<0.05）. Needle electromyography showed neurogenic damage in the affected muscles.Conclusion The electrophysiological results of patients with segmental zoster paresis can be classified into nerve plexus type， nerve root type，and single nerve type， and patients mainly have axonal damage. Nerve conduction combined with needle electromyography has an important value in the early diagnosis，muscle localization， and severity assessment of segmental zoster paresis.

Abstract:Objective To explore the mediating effect of fear of cancer recurrence on the family function and post-traumatic growth of young and middle-aged lymphoma patients.Methods The self-designed general information questionnaire，Post-traumatic Growth Inventory，simplified scale for fear of disease progression for cancer patients，and Family Assessment Device were used to investigate 280 lymphoma patients.Results The total post-traumatic growth score of lymphoma patients was （69.69±17.75），which was consistent with the previous survey results，and the average score of personal strength items was the highest； the total average score of the fear of disease progression scale for lymphoma patients was （40.01±9.08），the score was higher than that of patients with colorectal cancer and breast cancer；the total family function score of patients with lymphoma was （134.61±22.11），indicating that family function was at a normal level. There was a correlation among post-traumatic growth，fear of cancer recurrence，and family function. Fear of cancer recurrence played a mediating role between post-traumatic growth and family function，and the mediating effect accounted for 32.69% of the total effect.Conclusion In clinical nursing work，it is necessary to strengthen the role of family visits and home care，increase the enthusiasm and investment of family members in the treatment and rehabilitation of patients，improve the family function of patients，strengthen health education，and share successful anti-cancer experiences，thud reducing the patient's fear of cancer recurrence and improving the level of patient's post-traumatic growth.

Abstract:Type 2 diabetes mellitus（T2DM） is a common metabolic disease characterized by absolute or relative insulin deficiency clinically. Pancreatic β-cells are the core of insulin secretion and blood glucose control. In recent years，researches have found that palmitic acid can directly or indirectly induce pancreatic β-cell dysfunction，which is closely related to the occurrence and development of T2DM. However，the specific mechanisms are complex and have not been fully elucidated. Therefore，exploring the molecular mechanism of pancreatic β-cell dysfunction induced by palmitic acid has always been an urgent need in clinical research. This article reviews the research progress of the relationship between palmitic acid and pancreatic β-cells on molecular mechanisms related to oxidative stress，endoplasmic reticulum stress，inflammatory response，autophagy，proliferation，dedifferentiation，and mitochondria-associated endoplasmic reticulum membranes，and discusses new targets and ideas for improving pancreatic β-cell function clinically，in order to provide references for further researches on the mechanism and clinical treatment of T2DM.

Abstract:Cerebral ischemia-reperfusion injury（CIRI） is brain tissue damage secondary to ischemic stroke after blood flow recanalization and has extremely high fatality and disability rates. Exosomes are microvesicles with a double-layer lipid membrane structure released from cells into the extracellular space and play the roles of information transmission，targeted transportation，and regulation of various pathological and physiological functions through the membrane proteins，cytoplasmic proteins，and genetic materials they carry. Most cells inside the body can secrete exosomes，and exosomes and the microRNAs they carry play a positive role in alleviating neurological deficits and promoting cerebral angiogenesis after CIRI. Further studies on the protective effect of exosomes and their microRNAs on CIRI and its mechanism can provide new ideas for the bench-to-bedside translation of exosomes and the treatment of CIRI.

Abstract:Pulmonary hypertension（PH） is a disease characterized by progressive pulmonary vasoconstriction， pulmonary vascular remodeling，and right ventricular hypertrophy，finally leading to right heart failure. At present，there are still insufficiencies in the treatment methods for PH， and it is needed to develop new treatment strategies. In recent years，studies have been conducted on the therapeutic effect of mesenchymal stem cells（MSC） on PH and related mechanisms by scholars in China and globally，which has become one of the research hotspots of cell therapy for PH，and among them，the paracrine mechanism of MSC is considered to have an important therapeutic effect. Exosomes（EXO） isolated from MSC have the potential to inhibit pulmonary vascular remodeling and are considered as a new potential treatment method for PH. This article reviews the role and possible mechanism of MSC EXO in the treatment of PH，so as to provide a theoretical basis for the treatment of PH by MSC EXO in the future.

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