Abstract:Chondroitin sulfate proteoglycans（CSPGs） are components of the extracellular matrix of the central nervous system，and play key roles in the development，function maintenance，and pathological process of the central nervous system. After spinal cord injury，the expression of CSPGs at the injury site is significantly upregulated，which is mainly due to activated astrocytes at the lesion site. Upregulation of CSPGs can restrict axon regeneration，conduction，and re-myelination at the site of spinal cord injury，and can promote the inflammatory response in spinal cord injury，which impedes the recovery of nerve function after spinal cord injury. Therefore，inhibition of CSPGs may be an effective treatment to promote axonal regeneration and functional recovery after spinal cord injury. This study reviews the current status of research on CSPGs in spinal cord injury.

Abstract:Plasma exchange is an important treatment method in clinical practice and is widely used in the treatment of various clinical diseases such as neurological diseases，hematological diseases，and metabolic disorders，while it has not been widely used in China for the treatment of neuromyelitis optica-related optic neuritis. This article reviews the pathogenesis and clinical manifestations of neuromyelitis optica-related optic neuritis，the mechanism of plasma exchange in the treatment of neuromyelitis optica-related optic neuritis，and related precautions and adverse reactions，in order to provide a reference for clinical diagnosis，treatment，and prognostic evaluation.

Abstract:Monotherapy for immune checkpoint blockade（ICB） lacks efficacy in pancreatic ductal adenocarcinoma（PDAC），and rational combination therapy is an effective strategy to overcome ICB resistance in PDAC. Currently，combination therapies to overcome ICB resistance in PDAC mainly include the following：enhanced expression of programmed death receptor-ligand 1 or histocompatibility complex Ⅰ on the surface of PDAC cells；targeting key effectors in immune cells that play an immunosuppressive function to improve the immunosuppressive microenvironment of PDAC；combining the methods such as energy ablation，photodynamic therapy，and nano-material encapsulation to promote the release of tumor-associated antigens and stimulate immune activation. This article reviews the targets for ICB resistance and emerging methods in PDAC in recent years，so as to provide new ideas to address ICB resistance in PDAC.

Abstract:Objective To explore the effects of environmental enrichment（EE） on neural function，myelin-related protein expression of periventricular white matter and hippocampus in rats with hypoxic-ischemic brain injury（HIBD）.Method Seven-day-old SD rats were randomly divided into sham surgery group，model+standard environment group（HIBD+SE group） and model + environmental enrichment group（HIBD+EE group），and the HIBD model was established with the Rice-Vannucci method. The sham surgery group and the standard environment group were raised in the standard environment without any intervention，and the environmental enrichment group was treated with enriched environment from 8 to 18 days after surgery. At day18 after the surgery，Morris water maze was used to determine the escape latency of finding the hidden platform. Western blot was used to detect the expression of UGT8，a myelination-related protein，in periventricular white matter and hippocampus，and expression of oligodendrocyte differentiation gene OLIG2. RT-qPCR was used to detect the mRNA expression levels of OLIG2、GLTP、MYRF、UGT8 in periventricular white matter and hippocampus. Immunohistochemistry was used to detect the expression levels of NeuN，and immunofluorescence was used to detect the expression of myelin basic protein（MBP） in the corpus callosum.Result At 18 days after surgery，compared with HIBD+SE group，platform crossing times of the HIBD+EE group increased（P<0.01）. The HIBD+EE group showed higher expression of UGT8 mRNA and protein in the periventricular white matter and hippocampus compared to HIBD+SE group（P<0.05）. The expression of OLIG2，GLTP，MYRF mRNA in the periventricular white matter and hippocampus increased（P<0.05），and the expression of NeuN in CA1 region was higher than that of HIBD+SE group（P<0.01），the fluorescent intensity of MBP in corpus callosum region was higher than that in standard environment group（P<0.05）.Conclusion Environmental enrichment promotes the differentiation of oligodendrocyte lineages and myelin development in neonatal rats with HIBD，and improves learning and memory ability，which may be related to up-regulating UGT8 protein expression in periventricular white matter and hippocampus and increasing the number of neuronal mature cells.

Abstract:Objective To investigate whether aerobic exercise combined with resistance exercise can improve the diversity，abundance and structure of intestinal beneficial flora and its effect on the function of bone marrow endothelial progenitor cells（EPCs） in type 2 diabetic mice.Methods Forty 8-week-old male db/db mice were randomly divided into control group（DZ group）. Diabetic model + aerobic and resistance exercise group（combined exercise）（L group），diabetic model + fecal transplantation group（SY group），and diabetic model group（TJ group），with 10 rats in each group. Group L carried out aerobic exercise combined with resistance exercise for 8 weeks，aerobic exercise on Monday，Wednesday and Friday，and resistance exercise on Tuesday，Thursday and Saturday，6 days/W. In SY and TJ groups，the feces of mice in group L and DZ after 8 weeks of exercise were made into suspension，respectively，and then enema was given twice a day for 14 days. After the end of enema，the proliferation，migration，adhesion and angiogenesis ability of bone marrow EPCs in each group were detected. The EPCs phenotypes CD34 and CD31 were identified by flow cytometry，and the abundance and diversity of intestinal flora in feces were detected by 16SrRNA. ELISA was used to detect serum glucagon-like peptide1（GLP-1） in each group.Results At the class level，the abundance of Clostridia（54.60% vs. 32.21%） and Bacteroidia（47.63% vs. 36.39%） in the SY group was higher than that in the TJ group. The abundance of Gammaproteobacteria（0.30% vs. 12.57%） was significantly decreased（P=0.019，P=0.028，P=0.002）. Compared with DZ group，the abundance of Bacteroidia in SY group increased（47.63% vs. 38.5%），and the abundance of Bacilli decreased（2.56% vs. 12.36%），and the difference was statistically significant（P=0.021，P=0.039）. At the family level，the abundance of Lachnospiraceae（43.86% vs. 26.44%） and Oscillospiraceae（7.8% vs. 2.15%） in the SY group was significantly higher than that in DZ group（P=0.004，P=0.001）. Compared with the TJ group，The abundance of Lachnospiraceae（43.86% vs. 32.89%），Lactobacillaceae（9.89% vs. 3.8%） and Oscillospiraceae（7.8% vs. 2.21%） in the intestinal tract of SY group was significantly increased（P=0.045，P=0.006，P=0.001）. The proliferation，migration，adhesion and angiogenesis of EPCs in SY group were significantly higher than those in DZ group and TJ group（P=0.002，P=0.000，P=0.000，P=0.000，P=0.016，P=0.049，P=0.019，P=0.026）. The content of serum glucagon peptide-1（GLP-1）（pmmol/L） in DZ group SY and TJ group（1.90±0.22，2.82±0.40 and 2.24±0.49） was significantly different（F=31.082，P=0.000）. Compared with DZ group，the serum GLP-1 level in SY group was significantly increased（P=0.000）. Compared with TJ group，the serum GLP-1 level in SY group was significantly increased（P=0.000）. The correlation analysis between the number of intestinal flora and the function of endothelial cells showed that：Clostridia（r=0.696，P=0.041），Oscillospiraceae（r=0.697，P=0.037） Muribaculaceae was positively correlated with cell migration（r=0.753，P=0.019） and angiogenesis（r=0.810，P=0.008） in the endothelial group.Conclusion Aerobic exercise combined with resistance exercise may improve the function of EPCs in bone marrow of type 2 diabetic mice by increasing the abundance，diversity and structure of beneficial intestinal bacteria and increasing the expression of GLP-1.

Abstract:Objective To observe the effects of resveratrol on the proliferation and apoptosis of ovarian granulosa cells（OGCs） and the changes in the morphology and function of mitochondria in a rat model of polycystic ovary syndrome（PCOS）， and explore its possible protective mechanism.Methods Thirty-two young female Sprague-Dawley rats were randomly divided into two groups：polycystic ovary syndrome model group（n=24） and model control group（n=8）. Granulosa cells were extracted from the PCOS group and cultured in vitro，and treated with different concentrations of resveratrol. CCK-8 assay was used to measure the changes in cell activity； Mito Tracker Deep Red staining was used to observe the morphological changes in cell mitochondria；JC-1 was used to measure the changes in mitochondrial membrane potential；ATP kits were used to determine the changes in ATP content；spectrophotometry was used to measure the changes in enzymatic activity of caspase-3 and caspase-9，which are closely associated with apoptosis；finally，Western blot was used to measure the changes in the levels of caspase-3 and caspase-9 proteins.Results After treatment with resveratrol， the OGCs in the rat model of PCOS showed a significant increase in proliferation activity（P=0.000）. Mito Tracker Deep Red staining showed a significant increase in mitochondrial mass， with tubular or elongated shapes observed. ATP production and mitochondrial membrane potential in granulosa cells also significantly increased（both P=0.000）. In addition， resveratrol reduced the enzymatic activity and protein expression of caspase-3 and caspase-9（both P=0.000）.Conclusion Resveratrol inhibits the apoptosis of OGCs and promotes cell proliferation in the rat model of PCOS，which is associated with the improvement of mitochondrial morphology and function of granulosa cells by resveratrol.

Abstract:Objective To observe the effects of curcumin on the proliferation and apoptosis of colon cancer cell line HCT-116 as well as on the changes in mitochondrial morphology and function，and to explore the potential mechanism.Methods Colon cancer cell line HCT-116 was cultured in vitro and treated with different concentrations of curcumin（5，10，20，and 30 μmol/L）. Cell count kit-8 was used to observe the changes in cell proliferation，and the optimal concentration was identified. Flow cytometry was used for determination of the changes in cell apoptosis. Mitochondrial membrane potential assay kit JC-1 was applied to determine the changes in intracellular mitochondrial membrane potential. Electron microscopy and Mito Tracker Deep Red staining were used for observing the changes in mitochondrial morphology and structure. The changes in cellular adenosine triphosphate（ATP） and reactive oxygen species（ROS） were assessed by the corresponding assay kits. The changes in the activities and protein levels of apoptosis-related caspase-3 and caspase-9 enzymes were measured by spectrophotometry and western blotting，respectively.Results After curcumin treatment，the proliferation of HCT-116 cells was significantly inhibited in a concentration-time dependent manner，and the half maximal inhibitory concentration was 13 μmol/L. Curcumin not only remarkably increased the proportion of cells in early apoptosis，but also significantly reduced the intracellular mitochondrial membrane potential level in a concentration dependent manner. The electron microscopy results showed that curcumin caused obvious mitochondrial swelling in cells，including crista swelling，disappearance，membrane rupture，and vacuoles. Mito Tracker Deep Red staining showed a significant decrease in mitochondria number and fragmentation appearance. The intracellular ATP production was significantly decreased. In addition，curcumin also increased the activities and protein expression of caspase-3 and caspase-9 enzymes in cells.Conclusion Curcumin inhibits the proliferation of colon cancer HCT-116 cells and promotes cell apoptosis. The mechanism is related to the aggravation of mitochondrial morphology and function damage by curcumin.

Abstract:Objective To investigate the impacts of long non-coding RNA（LncRNA） myosin light chain kinase antisense RNA1 （MYLK-AS1） on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/stathmin 1（STMN1） axis.Methods HGC27 cells were grouped into negative control（NC） group，si-NC group，si-MYLK-AS1 group，si-MYLK-AS1+inhibitor NC group，and si-MYLK-AS1+miR-141-3p inhibitor group. The relationship among LncRNA MYLK-AS1，miR-141-3p，and STMN1 was determined by the dual-luciferase reporter gene assay；the expression of LncRNA MYLK-AS1 and miR-141-3p in HGC27 cells was measured by qRT-PCR；the CCK-8 method and flow cytometry were usde to determined the proliferation and apoptosis of HGC27 cells，respectively；the numbers of invading and migrating HGC27 cells were counted by Transwell；the protein levels of STMN1，E-cadherin，Vimentin，and N-cadherin in HGC27 cells were measured by Western blot.Results Compared with GES-1 cells，the levels of LncRNA MYLK-AS1 and STMN1 in HGC27 cells were significantly up-regulated（P<0.05），and the level of miR-141-3p was significantly down-regulated（P<0.05）. Compared with the NC group and si-NC group，the si-MYLK-AS1 group showed significant decreases in the optical density at 450 nm，numbers of migrating and invading cells，expression level of LncRNA MYLK-AS1，and protein levels of STMN1，N-cadherin，and Vimentin（P<0.05），as well as significant increases in the apoptosis rate，expression level of miR-141-3p，and protein level of E-cadherin（P<0.05）. However，the low expression of miR-141-3p attenuated the inhibition of HGC27 cell development by silencing LncRNA MYLK-AS1；LncRNA MYLK-AS1 regulated the miR-141-3p/STMN1 axis in a targeted manner.Conclusion Silencing LncRNA MYLK-AS1 may inhibit the expression of STMN1 by up-regulating miR-141-3p，thus affecting the proliferation，apoptosis，and invasion of gastric cancer cells.

Abstract:Objective To investigate the longitudinal association between hearing loss and depressive symptoms among the middle-aged and elderly people in China.Methods Related data were collected from the subjects who participated in at least two waves of China Health and Retirement Longitudinal Study in 2011-2020. Hearing loss was assessed based on self-reported data，while depressive symptoms were assessed using the Center for Epidemiologic Studies Depression Scale. The Kaplan-Meier curves and the log-rank test were used to compare the risk of depressive symptoms between groups. The Cox proportional hazards model was used to investigate the longitudinal association between hearing loss and depressive symptoms.Results A total of 12 952 subjects were enrolled in this study. At baseline，1 416 subjects（10.9%） reported hearing loss. During the median follow-up time of 5.42 years，5 498 subjects were found to have depressive symptoms. In the unadjusted model，the risk of depressive symptoms in the group with hearing loss at baseline was 1.34 times that in the group without hearing loss（P<0.05），and after adjustment for demographic variables，behavior and life styles，and health-related factors，hearing loss at baseline was still a risk factor for depressive symptoms（hazard ratio=1.21，95%CI=1.10-1.34）.Conclusion Hearing loss may increase the risk of depressive symptoms，and intervention for hearing loss can effectively reduce depression symptoms.

Abstract:Objective To analyze the distribution and drug resistance of pathogenic bacteria in 171 children with septic arthritis，and to provide a basis for clinical diagnosis and treatment.Methods Based on bacterial culture and drug sensitivity test results，we analyzed the distribution and drug resistance of pathogenic bacteria in patients with septic arthritis in Children’s Hospital of Chongqing Medical University from January 2012 to December 2022.Results A total of 82 strains of pathogenic bacteria were detected in 171 patients，including 74 Gram-positive （G⁺） and 8 Gram-negative strains（G^-）. The top three bacteria were Staphylococcus aureus（54 strains，65.9%），Streptococcus pyogenes（7 strains，8.5%），and Streptococcus pneumoniae（5 strains，6.1%）. The predominant pathogens were Staphylococcus aureus and Streptococcus pneumoniae in patients under two years of age （15 cases，71.4%），Staphylococcus aureus in patients between two and five years of age（9 cases，81.8%），and Staphylococcus aureus and Streptococcus pyogenes in patients over five years of age（40 cases，88.9%）. For all G⁺ bacteria，the rates of resistance to vancomycin and linezolid were 0%. The resistance rates of Staphylococcus aureus to benzoxacillin，amoxicillin/clavulanic acid，cefaclor，and flucloxacillin were <30%. The resistance rate of Streptococcus pyogenes to penicillin was 0%. Streptococcus pneumoniae was 20% resistant to penicillin and ceftriaxone. All G^- bacteria were 0% resistant to ceftazidime，cefepime，levofloxacin，ciprofloxacin，and imipenem.Conclusion Clinicians should choose appropriate antimicrobial drugs to treat septic arthritis according to the main pathogens and their resistance characteristics in local regions as well as the age distribution of patients.

Abstract:Objective To comprehensively analyze the influencing factors for the prognosis of patients with acute myocardial infarction（AMI） after percutaneous coronary intervention（PCI），to construct a prediction model and a prognosis scoring system，and to provide a reference for individualized vascular treatment in clinical practice.Methods A retrospective analysis was performed for all AMI patients who underwent PCI in The Second Affiliated Hospital of Nanchang University from January 2018 to June 2022，with the follow-up outcome of the onset of major adverse cardiovascular events（MACE） for the first time after surgery. The ten-fold cross-validated lasso regression analysis was used to determine the variables to be included in the model，and a random survival forest（RSF） model and a Cox proportional hazards model were constructed. The area under the ROC curve（AUC） and calibration curves were used to evaluate the performance of the model，and a risk calculator was developed according to the fitting results of RSF model.Results A total of 3 880 patients with AMI were finally included in the study，among whom 473（12.2%） experienced MACE within one year after surgery. Lasso regression obtained 15 variables including sex，type of AMI，and hypertension，and the multivariate Cox regression analysis showed that diabetes，low left ventricular ejection fraction（30%~40%），and degree of vascular stenosis were the risk factors for postoperative MACE. In the validation set，the RSF and Cox models had an AUC of 0.774（95%CI=0.761~0.787） and 0.597（95%CI=0.581~0.613），respectively. The calibration curves showed that the model had a relatively high accuracy in predicting the risk of MACE within one year，and RSF score with the optimal cut-off value of 133 could also accurately distinguish the cumulative risk of MACE（P<0.001）.Conclusion The RSF model and the scoring system constructed based on the above factors can effectively predict the risk of postoperative MACE and perform risk stratification，thereby helping cardiovascular physicians to formulate individualized treatment regimens in clinical practice.

Abstract:Objective To explore the potential categories of behavioral characteristics of children with acute leukemia using latent class analysis and associated influencing factors，and to provide evidence for developing precise intervention strategies for behavioral problems in children with leukemia.Methods We selected 202 children with acute leukemia and their caregivers by convenience sampling in the Department of Hematology of Children’s Hospital of Chongqing Medical University for a survey using the General Information Questionnaire， Rutter Child Behavior Questionnaire，General Family Functioning Scale， and Social Support Rating Scale. The behavioral characteristics of children with acute leukemia were categorized using a latent class analysis， and the effects of various factors on different categories were analyzed using a multinomial logistic regression analysis.Results TThe behavioral characteristics of children with acute leukemia were classified into three types： impulsive-unfriendly type（50%），emotional-somatic disorder type （33.66%），and well-behaved type（16.34%）. The multinomial logistic regression analysis showed that unhealthy family functioning and male were more frequently associated with the impulsive-unfriendly type compared with the well-behaved type； unhealthy family functioning，male，and being in the treatment period were more frequently associated with the emotional-somatic disorder type compared with the well-behaved type；and children in the treatment period were prone to developing the impulsive-unfriendly type compared with the emotional-somatic disorder type.Conclusion Children with acute leukemia have distinct types of behavioral features， which are closely related to family functioning， sex， and disease stage. Therefore， for different behavioral types， targeted individualized interventions should be developed to prevent or reduce the occurrence of behavioral problems in children with acute leukemia， improving their social adaptability and quality of life.

Abstract:Objective To investigate the different characteristics of urinary tract microbiome in simple acute or recurrent lower urinary tract infection，and to explore the new directions for the diagnosis and treatment of recurrent urinary tract infection.Methods A total of 53 female patients with simple lower urinary tract infection who were treated in The First Affiliated Hospital of Chongqing Medical University and People’s Hospital of Fengjie from May 2020 to September 2021 were enrolled and divided into acute group with 27 patients and recurrent group with 26 patients. Urine samples were collected for culture，and 16s ribosomal ribonucleic acid next-generation sequencing was used to detect the microbiome in urine.Results At the phylum level，the mean value of OD1 was 0.004 9±0.002 6 in the acute group and 0.002 8±0.001 8 in the recurrent group，and the mean value of Planctomycetota was 0.003 5±0.001 9 in the acute group and 0.002 3±0.002 0 in the recurrent group，with significant differences between the two groups（t=3.444 and 2.160，P=0.001 and 0.035）；at the genus level，as for the genera with a relatively high mean value of relative abundance in the acute group，the mean value of Ochrobactrum was 0.026 9±0.010 9 in the acute group and 0.017 1±0.010 3 in the recurrent group，with a significant difference between the two groups（t=3.372，P=0.001）. There was no significant difference in the median of α diversity between the acute group and the recurrent group，and there was also no significant difference in the median of matrix distance for β diversity between the acute group and the recurrent group［0.277 （0.225，0.361） vs. 0.319（0.240，0.519），F=2.259，R=0.042，P=0.027］；α-Proteobacteria was one of the classification units with a significant difference in median relative abundance between the two groups.Conclusion There are significant differences in the diversity，relative abundance，and dominant flora of the microbiome between the acute group and the recurrent group.

Abstract:Objective To investigate the adverse pregnancy outcomes of pregnant women with a singleton or twin pregnancy diagnosed with intrahepatic cholestasis of pregnancy（ICP）.Methods Clinical data were collected from 2886 pregnant women with ICP who delivered in The First Affiliated Hospital of Chongqing Medical University from 2018 to 2021，and based on the inclusion and exclusion criteria，1992 cases were selected for subsequent analysis. The pregnant women with ICP were divided into singleton pregnancy group and twin pregnancy group based on the number of fetuses delivered. Baseline characteristics were analyzed for both groups，and propensity score matching（PSM） was used to adjust any baseline differences between the two groups. The chi-square test was used to analyze the adverse pregnancy outcomes of pregnant women with ICP.Results Before PSM，there were significant differences between the two groups in all baseline characteristics except pre-pregnancy body weight（P=0.064） and smoking（P=0.129），while there were no significant differences between the two groups after PSM. As for maternal outcomes，compared with the singleton ICP group，the twin ICP group had significantly higher incidence rates of gestational hypertension（8.40%；P=0.004），preeclampsia（10.9%；P=0.035），placenta accreta spectrum（15.1%；P=0.004），and polyhydramnios（5.30%；P=0.010）； compared with the twin ICP group，the singleton ICP group had significantly higher incidence rates of preterm rupture of membranes（27.0%；P=0.002） and oligohydramnios（12.3%；P=0.022）. In terms of neonatal outcomes，compared with the singleton ICP group，the twin ICP group had significantly higher incidence rates of preterm birth（38.9%；P<0.001），fetal anomaly（3.90%；P=0.004），low birth weight（44.6%；P<0.001），and admission to the neonatal intensive care unit（24.91%；P<0.001）；compared with the twin ICP group，the singleton ICP group had a significantly higher incidence rate of fetal macrosomia（4.91%；P=0.007）.Conclusion Compared with the pregnant women with ICP and a singleton pregnancy，the pregnant women with ICP and twin pregnancy tend to face more severe adverse pregnancy outcomes.

Abstract:Objective To analyze the characteristics of computed tomography（CT） parameters in patients with advanced Kashin-Beck disease（KBD） knee arthritis and to provide a reference for the diagnosis of this disease and the preoperative planning of knee arthroplasty.Methods Patients diagnosed with KBD knee arthritis in our hospital from January 2018 to December 2022 were retrospectively analyzed，and the patients with advanced knee osteoarthritis in the same period were selected as controls. The CT parameters of patients’ knee joint were analyzed by Mimics software for measurement of knee-joint osteophyte volume，the number of loose bodies within the articular cavity，femoral mediolateral（FML） diameter，femoral medial anteroposterior（FMAP） diameter，femoral lateral anteroposterior（FLAP） diameter，tibial mediolateral（TML） diameter，tibial anteroposterior（TAP） diameter，tibial medial anteroposterior（TMAP） diameter，and tibial lateral anteroposterior（TLAP） diameter，and the FML/FMAP，FML/FLAP，TML/TAP，and TMAP/TLAP ratios were calculated.Results A total of 70 patients were included，with 32 patients in the KBD group and 38 patients in the osteoarthritis group. There were no significant differences in sex distribution，average age，and body mass index between the two groups （P>0.05）. In the KBD group，patients often developed multi-compartment arthritis in the knee joint with a large number of osteophytes around the joint，and significant subchondral osteosclerosis. The KBD group had significantly higher volume of osteophyte，number of loose bodies within the articular cavity，and FML/FLAP and TMAP/TLAP ratios than the control group（P<0.05）.Conclusion Patients with advanced KBD knee arthritis have more significant degenerative signs and smaller FLAP and TLAP diameters. These imaging characteristics can be useful for the preoperative planning of knee arthroplasty in patients with advanced KBD knee arthritis.

Abstract:Objective To compare the therapeutic effects of different medication regimens in patients with hypertension with different blood pressure levels based on patient reported outcomes（PROs），and to provide a reference for clinicians to choose medication regimens.Methods A total of 625 community patients with hypertension who were taking antihypertensive medications underwent a baseline PRO measure and three repeated-measures of PRO（once every three months）. Repeated-measures analysis of variance （ANOVA） was used to compare the PRO of six combinations generated with two medication regimens（single medications and combined medications） and three blood pressure levels（grade-1 hypertension，grade-2 hypertension，and grade-3 hypertension） at different time points.Results Repeated-measures ANOVA results showed that patients with hypertension having different medication regimens and blood pressure levels had varying degrees of fluctuations in PRO scores across three repeated measures. For patients with grade-1 hypertension on single medications，PRO scores in physical health，mental health，social health，spiritual/faith-related health，and the specific module fluctuated downward. For both patients with grade-2 hypertension and those with grade-3 hypertension on single medications，the scores in mental health，social health，and spiritual/faith-related health fluctuated upward. There were no significant differences in PRO scores in physical health among grade-1，-2，and -3 hypertensive patients on combined medications. There was a downward trend in mental health-related PRO score in patients with grade-2 hypertension on combined medications. The PRO score in social health showed a fluctuating upward trend in patients with grade-3 hypertension on combined medications. For patients with grade-1 hypertension and those with grade-2 hypertension on combined medications，the PRO score in spiritual/faith-related health fluctuated downward and upward，respectively. There was a downward trend in the specific module score of patients with grade-2 hypertension on combined medications. The total PRO score declined in patients with grade-1 hypertension and fluctuated upward in patients with grade-2 and grade-3 hypertension，regardless of single or combined medications.Conclusion Patients with different grades of hypertension have varying PRO scores with single and combined medications，and clinicians can refer to patients’ PROs when choosing medication regimens.

Abstract:Objective To investigate the efficacy and safety of teniposide superselective intra-arterial chemotherapy combined with bevacizumab in the treatment of recurrent high-grade glioma.Methods According to the treatment method，79 patients with recurrent high-grade glioma were divided into experimental group with 37 patients （teniposide intra-arterial chemotherapy combined with bevacizumab） and control group with 42 patients （bevacizumab monotherapy）. The two groups were compared in terms of objective response rate，disease control rate，and the incidence rate of adverse reactions，and the impact of the gene promoter methylation status of O⁶-methylguanine-DNA methytransferase on disease control rate was analyzed.Results The objective response rate was 43.2% in the experimental group and 21.4% in the control group（P=0.038），and the disease control rate was 78.4% in the experimental group and 54.8% in the control group（P=0.027）. Compared with the control group，the experimental group had significantly higher incidence rates of the adverse reactions such as mild gastrointestinal reactions（P=0.044），headache（P=0.044），and leucopenia（P=0.026）. Compared with the control group，the experimental group had a significantly higher disease control rate in the population aged <60 years（P<0.05）.Conclusion Teniposide superselective intra-arterial chemotherapy combined with bevacizumab has better efficacy than bevacizumab monotherapy in the treatment of recurrent high-grade glioma and thus holds promise for further clinical application.

Abstract:Objective To analyze and summarize the clinical effectiveness of total hip arthroplasty（THA） with structural femoral head autografting for acetabular reconstruction in the treatment of adult patients with revised Crowe type ⅣB developmental dysplasia of the hip（DDH）.Methods We included 26 patients（25 males and 1 female） who were diagnosed with type ⅣB DDH according to the revised Crowe classification and underwent THA with structural femoral head autografting for acetabular reconstruction in the Department of Joint Surgery，Qilu hospital of Shandong University Dezhou hospital from August 2015 to March 2023. The surgical time，intraoperative blood loss，intraoperative blood transfusion volume，postoperative blood transfusion volume，postoperative hemoglobin content，surgical complications，and bone healing time were recorded. Anteroposterior pelvic X-rays were taken to examine prosthetic position，bone ingrowth，prosthetic loosening，and bone healing. The visual analogue scale（VAS） score was used to assess the intensity of pain in the hip. The Harris hip score and the Western Ontario and McMaster Universities Osteoarthritis Index（WOMAC） were used to evaluate hip function and clinical effectiveness.Results For all the cases，the mean follow-up time was （9.73±8.35） months，the mean intraoperative blood loss was （715.38±143.37） mL，the mean intraoperative blood transfusion volume was （415.38±282.41） mL，the mean surgical time was （118.62±18.27） min，and the mean postoperative blood transfusion volume was （192.31±236.51） mL. All the patients had good healing between the subtrochanteric region，femoral head autograft，and false acetabulum. The hip VAS score showed a significant decline from （6.73±0.45） points before operation to （1.73±0.53） points at the last follow-up after operation（P<0.001）. The ranges of motion of the hip joint were all significantly improved. The Harris hip score was increased significantly from （24.27±1.66） points before surgery to （74.77±2.89） points at the last follow-up after surgery（P<0.001）. The WOMAC score showed a significant decrease from （130.08±5.72） points before surgery to （67.85±3.23） points after surgery（P<0.001）.Conclusion THA with structural femoral head autografting for acetabular reconstruction is a relatively simple procedure to perform，with good fixation，safety，and effectiveness for the treatment of revised Crowe type ⅣB DDH.

Abstract:Objective To investigate the clinical effect of suspension fixation combined with Sigmoid colon folding in the treatment of complete rectal prolapse.Methods A total of 50 patients with complete rectal prolapse who were admitted to The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from February 2017 to May 2023 were enrolled，and according to the surgical procedure，they were divided into control group with 20 patients and observation group with 30 patients. The patients in the control group underwent Delorme surgery，and those in the observation group underwent suspension fixation combined with Sigmoid colon folding for rectal prolapse. The two groups were compared in terms of clinical outcome，clinical recovery on day 30 after surgery，complications，recurrence rate at 1 year after surgery，and anorectal pressure before and after treatment，and functional efficacy was evaluated based on Wexner fecal incontinence score.Results The observation group had a higher overall cure rate than the control group（P=0.012）. On day 30 after surgery，compared with the control group，the observation group had significantly lower scores of anal pendant expansion（t=2.300，P=0.026），stool control ability（t=2.299，P=0.026），and anal moisture（t=2.205，P=0.032）. At 1 month after surgery，the observation group had a significantly lower overall incidence rate of complications than the control group（χ²=6.255，P=0.012）. On day 90 after surgery，both groups had a significant reduction in rectal sensory volume（t=20.634，P<0.001） and significant increases in maximum resting pressure（MRP） and maximum squeeze pressure（MSP），and the observation group had significantly higher MRP（t=-11.486，P<0.001） and MSP（t=-7.358，P<0.001） than the control group. After 1 year of follow-up，the observation group had a lower recurrence rate than the control group（P=0.033）. At 3 months after surgery，there was no significant difference between the two groups in the total score of passing of flatus，liquid stool，solid stool，pad wearing，and lifestyle change（t=0.197，P=0.845）.Conclusion Suspension fixation combined with Sigmoid colon folding has a better clinical effect than Delorme surgery in the treatment of complete rectal prolapse，with less intraoperative trauma，fewer complications，faster recovery，and a lower long-term recurrence rate.

Abstract:Objective To investigate the risk factors of fever in hospitalized patients with acute gout.Methods The data of 185 hospitalized patients with acute gout meeting the inclusion criteria were retrospectively analyzed and divided into moderate and high fever group（n=25），low fever group（n=25） and no fever group（n=135） according to body temperature. The clinical data，inflammatory indicators and medication data were compared. Then the two groups were divided into knee pain group（n=69） and pain free group（n=116） according to the presence or absence of knee pain.The risk factors of hospitalized gout fever were analyzed by binary Logistic regression.Results The white blood cell count in 3 groups was（9.2±2.3 vs. 8.6±3.3 vs. 7.7±2.5，P=0.022），neutrophil percentage（77.7±4.0 vs. 70.5±10.6 vs. 67.7±12.1，P=0.001），pain VAS score［3（3.0，4.0） vs. 2（2.0，3.0） vs. 2（1.5，3.0），P=0.001］，c-reactive protein（102（49，137） vs. 36（22，83） vs. 15（5，53），P=0.001） and ESR levels50（41，66） vs. 28（16，45） vs. 27（14，46），P=0.001］ were statistically significant. The white blood cell count and neutrophil percentage in the high fever group were higher than those in the no-fever group（P=0.012，P=0.001）. The pain VAS score，CRP and ESR in moderate and high fever group were higher than those in no fever group and low fever group（P=0.001，P=0.001，P=0.001 and P=0.014，P=0.033，P=0.011）.The proportion of NSAIDS used in low-fever group was higher than that in no-fever group（92% vs. 68.1%，P=0.015） and medium-high fever group（92% vs. 60.0%，P=0.008）. In the three groups，knee pain（56.0% vs 48.0% vs. 31.9%，P=0.036），first toe joint pain（6.0% vs. 0.0% vs. 25.9%，P=0.007），upper limb joint pain（40.0% vs. 16.0% vs. 17.8%，P=0.034） and multiple joint involvement（56.0% vs. 24.0% vs. 25.9%，P=0.008） were statistically significant. Proportion of ESR［43（21，56） vs. 25（14，41），P=0.001］，CRP［45（11，115） vs. 17（5，49），P=0.001］ and local treatment of joint cavity in patients with knee pain［（58.0% vs. 6.9%），P=0.001］ were higher than those without knee joint involvement. Logistic regression analysis showed that leukocytes（OR=1.171，95%CI=1.037-1.323，P=0.011），neutrophil percentage（OR=1.053，95%CI=1.020-1.087，P=0.002），CRP（OR=1.015，95%CI=1.008~1.021，P=0.001），ESR（OR=1.023，95%CI=1.008-1.039，P=0.003），pain VAS score（OR=1.674，95%CI=1.228-2.282，P=0.001），knee pain（2.428，95%CI=1.252-4.709，P=0.009） was a risk factor for hospitalized gout fever. First toe joint pain（OR=0.233，95%CI=0.068-0.804，P=0.021） was a protective factor for hospitalized gout fever. The increase of CRP level（OR=1.011，95%CI=1.005-1.018，P=0.001） and pain VAS score（OR=1.446，95%CI=1.035-2.019，P=0.031） were independent risk factors for hospitalized gout fever.Conclusion The proportion of hospitalized acute gout patients with fever was 27%（50/185），and patients with fever had higher inflammation levels and pain scores，a higher proportion of knee pain and multi-joint pain，and lower rates of first toe joint pain，which required stronger anti-inflammatory and analgesic therapy. In particular，high CRP level and high pain VAS score were independent risk factors for hospitalized gout fever.