Abstract:Objective: To analyze the clinical features, pathological changes and treatment of acute interstitial nephritis (AIN), improve the understanding and clinical diagnosis of AIN. Methods: 199 cases of children AIN in our hospital during the period of January, 2010 to December, 2017 were analyzed about the clinical features, auxiliary examination and treatment outcome. Results: There were 180 cases of infection-induced AIN in199 cases, accounting for 90.4%, respiratory infections were the most common (66.8%), followed by digestive infection. The main pathogens are virus infection, including EB virus, Coxsackie virus, Respiratory syncytial virus and so on. 125 cases of DAIN, Non-steroidal anti-inflammatory drugs (52.5%) and antibiotics (30.3%) were the most common. 19 cases (9.5%) of Non-infectious diseases, including bee stings, poisoning, Surgery and so on. The main clinical manifestations included 117 cases of fever (58.8%), 60 cases of vomiting (39.2%), 46 cases of abdominal pain (30.2%), 35 cases of diarrhea (17.6%), 9 cases of eosinophilia (4.5%), and 9 cases (4.5%) of rash. renal damage include 147 cases (73.9%) of hematuria, 86 cases (43.2%) of edema, 82 cases (41.2%) of oliguria or anuria. Typical triads are not seen in this study. 69 patients (34.7%) had ARF, 50 patients (26.1%) had an increase in 24h urinary protein (mean 0.49 ± 0.45g/24 h). Urine routine include hematuria (73.9%), proteinuria (64.3%), leukocyturia (28.6%) . Four cases of renal biopsy were performed with tubulointerstitial lesions, and the glomerulus and renal vessels were basically normal. All patients were treated with removal cause and symptomatic treatment, 46 patients were treated with blood purification because of ARF or poisoning, 6 of them were combined with glucocorticoid, renal function and Urine tests returned to normal in the short term. Conclusion: The main cause of AIN in children is infection, mainly viral infection, followed by drugs. The clinical manifestations is unexplained renal function decline and abnormal urine test. Acute renal failure may occur in severe cases .Timely diagnosis and remove the cause of the key to treatment, blood purification and short-term glucocorticoid therapy if it is necessary, most of them have a good prognosis.

Abstract:#$NLObjective：To detect the expression of microRNA-496(miR-496) in ovarian serous carcinoma, analyze its clinical significance, analyze its correlation with homologous heteroprotein SIX1(SIX1). Methods：75 cases of ovarian serous carcinoma were selected as the observation group, 75 cases of ovarian serous cystic adenoma were selected as the control group. Expression of miR-496 was detected by real-time fluorescence quantitative PCR in two groups. Expression of PCNA and BAX was detected by immunohistochemical method in the observation group. Expression of SIX1 was detected by Western Blot method in the observation group. Results：Expression of miR-496 was lower in the observation group than that in the control group(P<0.05). The expression of miR-496 was statistically significant in different tumor maximum diameter, histological grade, vascular recidivism, bilateral occurrence, lymph node metastasis and different TNM stages in the observation group(P<0.05). Expression of miR-496 is related to survival time(P<0.05). Negative correlation were found between miR-496 and PCNA(r=-0.54,P=0.018), miR-496 and SIX1(r=-0.58, P=0.013), positive correlation were found between miR-496 and BAX(r=0.52,P=0.011). Conclusion：Expression of miR-496 of ovarian serous carcinoma is decreased, which has a certain regulatory effect on the formation and development of the tumor. Expression of miR-496 has a certain significance for judging the prognosis. MiR-496 not only regulates cell proliferation and apoptosis, but also may play a role in the regulation of SIX1.

Abstract:objective To identify pathogenic mutation of the TSC1 and TSC2 genes in a family with tuberous sclerosis, and to analyze clinical data of this mutation. Methods Peripheral venous blood samples and clinical data of the patients and her parents were collected. Genomic DNA was extracted. All coding exons of the TSC1 and TSC2 genes were amplified by polymerase chain reaction and subjected to direct sequencing. The clinical data of all patients with this mutation in internal reports were concluded. Result The patient has presented angiofibroma of the right waists, left popliteal fossa and face for more than twenty years. She also had epilepsy but no mental retardation. A nosense mutation was c.1513C>T was detected in exon 14 of the TSC2 gene, which had led to a premature stop eodon TAG after the 505th amino acids.The same mutation was not found in her parents and 25 unrelated healthy controls. Conclusion The nosense mutation c.1513C>T in the TSC2 gene may be responsible for the disease in the patients and the clinical phenotype of this mutation was very diverse.

Abstract:Objective: To investigate the expression of matrix metalloproteinases 9 (MMP-9) in osteosarcoma tissues and the effect of MMP-9 on the progression of osteosarcoma and its clinical significance. Methods: The expression of MMPs’ family in 5 osteosarcoma specimens and 5 normal bone tissues was detected by real-time PCR. shRNA was used to knockdown the expression of MMP-9 in Saos2 cells. The proliferation, invasion and migration of the cells after transfection were detected for the effects of MMP-9 down-regulation. According to the expression of MMP-9 in osteosarcoma, 64 follow-up cases were grouped and analyzed for survival rate. Results: Screening of MMPs’ family revealed that the expression of MMP-9 in osteosarcoma tissues was significantly higher than that in normal bone tissues (t=12.040, P=0.000). In cell proliferation, invasion and scratch test, there was no significant difference between blank control group and negative control group. But compared with negative control group, down-regulation of MMP-9 in Saos2 cells markedly repressed cell proliferation，decreased invasion and migration (t=4.954, P=0.008; t=6.360, P=0.003; t=3.965, P=0.017) . In addition, a survival analysis in 64 follow-up cases showed that the overall survival rate of the high-MMP-9 group was significantly lower than that of the low-MMP-9 group (χ2=4.169，P=0.041). Conclusion: The high expression of MMP-9 in osteosarcoma tissue is closely related to the proliferation, invasion and migration of osteosarcoma, which has certain implication for the clinical prognosis of patients.

Abstract:With the aggravation of population aging, Alzheimer"s disease (AD), as an irreversible neurodegenerative disease, plagues people all over the world and brings heavy burden to families and society.Acupuncture play an increasingly important role in the clinical treatment of AD with its advantages of safety, simplicity and good effect.This paper summarizes the molecular biology research of acupuncture in the treatment of AD in the past five years.It summarizes the effects of acupuncture on AD through regulating the level of abnormal proteins, regulating the central cholinergic system, protecting neurons, regulating energy metabolism, inhibiting inflammatory response, and increasing the level of autophagic activity. It provides a theoretical basis for the clinical application of acupuncture in the treatment of AD.

Abstract:Objective:To study the feasibility and regulation mechanism of recombinant human parathyroid hormone(rhPTH) to promote orthodontic tooth movement after maxillary anterior osteotomy. Methods: Forty-eight rabbits were used to a model of anterior maxillary osteotomy and the orthodontical movement of right maxillary first molars. They were randomly divided into experimental group and control group. The group received subcutaneous injection of rhPTH 20 μg/kg or normal saline every other day. On the 5th, 7th, 14th, and 21th days, the animals were sacrificed respectively. Then the mesial movement distance of the right maxillary first molars was measured. The TRAP staining, immunohistochemistry and fluorescence quantitative PCR were performed on the proximal periodontal tissues of the moving tooth. Results: At the same stage, the moving distance and speed of the first molars in the experimental group were faster than those in the control group (P<0.05). In the mesial periodontal tissue of right maxillary first molars, the osteoclast counts of the experimental group was significantly larger than that of the control group (P<0.05). Through immunohistochemistry and fluorescence quantitative PCR Testing, In the mesial periodontal tissue of right maxillary first molars, the expression of Sclerostin (SOST) mRNA and protein in the experimental group was significantly higher than those in the control group (P<0.05), while the bone morphogenetic protein-2(BMP-2) mRNA and protein in the experimental group were lower than that of the control group (P<0.05). Conclusion: In the proximal pressure side of the orthodontic tooth after maxillary anterior osteotomy, subcutaneous injection of rhPTH may increase the alveolar bone remodeling to accelerate the movement speed of orthodontic tooth by up-regulating the expression of SOST and reducing the expression of BMP-2.

Abstract:Objective: To analyze the clinical features of pancreatic dermoid cysts. Methods: The clinical and imaging data of a patient with dermoid cyst of pancreatic head were reported and reviewed with related literature. Results: The patient was found to have a head position on the head of the pancreas and no clinical symptoms were admitted to the hospital. After the relevant examination, the solid pseudopapillary tumor was considered. After the surgical resection and pathological examination, the pancreatic dermoid cyst was diagnosed. There was no recurrence after follow-up. Conclusion: Dermoid cyst of Pancreatic head is a very rare benign tumor in clinical practice. The imaging findings are not specific and the diagnosis is difficult.

Abstract:Objective This study aimed to assess the correlation of plasma miR-126 with the risk of acute respiratory distress syndrome (ARDS), severity, inflammation level and prognosis in sepsis patients. Methods One hundred and seventy-two sepsis patients were enrolled in this study. The peripheral blood samples of patients were collected within 24 hours after hospital admission, and plasma was separated for miR-126 expression detection by quantitive polymerase chain reaction. The expressions of inflammatory cytokines (including TNF-α, IL-1β, IL-6 and IL-17) levels in plasma were evaluated by enzyme-linked immuno sorbent assay (ELISA). Meanwhile, the 28-day mortality was recorded as well. Results There were 50 (30.8%) patients who had ARDS during hospitalization, and plasma miR-126 expression was elevated in ARDS patients compared with non-ARDS patients (P<0.001), and it could differentiate ARDS patients form non-ARDS patients with AUC of 0.741 (95%CI: 0.666-0.815) according to the receiver operating characteristic curve (ROC) analysis. The multivariate logistic regression model analysis revealed that plasma miR-126 (high vs. low) independently predicted higher risk of ARDS (P<0.001). In addition, in the total sepsis patients, plasma miR-126 expression was positively correlated with levels of Scr (P=0.004), CRP (P<0.001), PCT (P<0.001), APACHE II score (P<0.001), SOFA score (P<0.001), and TNF-α (P=0.001), IL-1β (P=0.002), IL-6 (P=0.011) as well as IL-17 (P=0.002) expressions in plasma. Moreover, the plasma miR-126 was upregulated in non-survivors compared with that in survivors (P<0.001), ROC curve analysis revealed that it could distinguish survivors form the non-survivors with AUC of 0.686 (95%CI: 0.601-0.771). Conclusion Higher circulating miR-126 expression is correlated with higher risk of ARDS and could serve as a biomarker for disease surveillance and prognosis in sepsis.

Abstract:回顾性分析我院确诊的1例脂质沉积性肌病，结合文献报道总结脂质沉积性肌病的临床表现和病理特点, 并分析容易误诊为多发性肌炎的原因。